LOCUS       CP090260                3555 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E-R791-1 plasmid punnamed2, complete
            sequence.
ACCESSION   CP090260
VERSION     CP090260.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394356
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Chen,J., Liu,C. and Zhang,R.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Chen,J., Liu,C. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:27:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,005
            CDSs (total)                      :: 4,886
            Genes (coding)                    :: 4,745
            CDSs (with protein)               :: 4,745
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 90 of 141
            Pseudo Genes (internal stop)      :: 22 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E-R791-1"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed2"
                     /geo_loc_name="China"
                     /collection_date="2020"
     gene            complement(43..366)
                     /locus_tag="LZT32_00005"
     CDS             complement(43..366)
                     /locus_tag="LZT32_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="UIL79025.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            650..844
                     /locus_tag="LZT32_00010"
     CDS             650..844
                     /locus_tag="LZT32_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIL79026.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1650..2375)
                     /locus_tag="LZT32_00015"
     CDS             complement(1650..2375)
                     /locus_tag="LZT32_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="UIL79027.1"
                     /translation="MASMETEQQPQQTTEQIEVKTKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKNASIDNVFWFSAKPSHLSRAINENYQNYTNALTECGVDVIGGRF
                     KKKNVTCEADNGCGGVFHKHEEKESDVNLAISLVSGACNDEYDIAIIITADSDLCPPIK
                     YVRERFPNKELWLVAPPGRIRRATDLCSLSSRSMEIKNDALRACCMRDEFYKDGVLVAR
                     RPQEWRAVANTRQRRARSR"
                     /name="NYN domain-containing protein"
     gene            2696..3310
                     /locus_tag="LZT32_00020"
     CDS             2696..3310
                     /locus_tag="LZT32_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UIL79024.1"
                     /translation="MSTKNAQKEQKKESLITTHWGDDATIHGWTAIPNSLLMLQGDLGI
                     GSTEMCILLNILMHQWPESGESISFPSIGTIASRIGVSKRTIQRGVSNLEALGILTRYQ
                     STRNDPLTNGANIFDSTPLKEYLNKKAKGITISNSNKKKERKKTGITPNIKLPRICPKC
                     LKTKATSHEEIVKFFGVRKTMAGEIINSYCKECRASEKDLF"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(376..615)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          880..1401
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.437"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(47..366)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.86e-62"
ORIGIN
        1 ggcgttcata ccgtctgttt tcgacagttt ccctccggga agctaatctg ccataagcct
       61 ggataacagg gcccggtgat actccgtaat agctatcagc acctccgcat actctctgtc
      121 cccgacacgc ttgccgtcga taaagagttt tttctgcaac gcccccagtc gcatgacttc
      181 tttcagcact cggtcatcag tcagtgaatt aactttctta ccgagagctg ccgcccgtaa
      241 aaaaccagaa acggtcttgc cgcattcagc agcccttttt ctgatttcct ggtcttcttc
      301 tgccgtcaga cgaacagggc gactgatagc ccgcctgcgc gtattacttc cacttcgttt
      361 tgtcataaaa catggctccg tatctgacat gggtgtcggg gcaaagccct gaccagggta
      421 attgtaatag cgtgcatgta tgcgcggtat aacaattgca catcctgtcc tgtcagcaag
      481 ctcgaattac cgacaaaagc acggcagttt tttggcagtg acagtgttgt ctgacagaaa
      541 tgcccgtcaa agagtcagac agcagaatac cggcggtaaa acagtcgaaa agtggtactc
      601 gatataatca cacaacgcag atgacagaaa gaacggagaa aacagaaaga tgaacaagca
      661 gcagcaaacc gtactcaaca tggcgggatt cataaaaagc cagagcctga cgctgctcga
      721 aaaactggac gcacttgatg ctgacgaaca ggccgccatg tgtgagaagc tgcacgaact
      781 cgcggaagaa cttcagaaca gcatacagac acgctttgaa gcggaaaatc gcaccgggat
      841 ataacgcacc cggacaggat gcagaacggc atagcggagc gtaaggagaa aatgccacgt
      901 cagacgctct tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
      961 agcggtgtca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1021 cggaaagaac atgtgagcaa aaagcaaagc accggaacaa gccagcgccg atgccgtttt
     1081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1261 tggcgctttc tcatagctca cgctgttggt atctcagttc ggtgtaggtc gttcgctcca
     1321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1381 atcgtcttga gtccaacccg gaaagacacg acttaacgcc actggcagca gccattggta
     1441 actgatttag aggagttgag tcttgaagtt atgcacctga taaggctaaa ctgaaagaac
     1501 aagttttggc gactgtgctc ctcaaagcca gttacctcgg ttcaaagagc gccaactcta
     1561 tgaaccttcg aaaaaccgcc cgccaaggcg gttttttcgt tttcaaccta gttagattaa
     1621 tctccttgcg ctagctgcgt caacaaagcc taacgacttc tagcccttct ttgcctggta
     1681 ttggcaacag ctcgccattc ctgaggcctt ctggctacaa gtactccatc tttgtagaac
     1741 tcatcacgca tacagcaggc tctcaaagca tcattcttga tctccatact tctcgatgac
     1801 agagagcaga gatctgtcgc tcgcctgatt ctccccggag gagctacaag ccaaagctct
     1861 ttattaggga atcgctctct tacgtattta ataggggggc ataagtcaga atccgcagtg
     1921 ataataatgg ctatatcata ctcatcatta cacgcaccag acactagact tatagccaaa
     1981 ttaacatcgg actctttttc ttcatgctta tggaaaactc caccacatcc attatcagct
     2041 tcgcacgtta cattcttttt tttaaaacgc ccaccgataa catcaacacc acattcggtt
     2101 aaagcgttgg tgtaattttg ataattttca tttatggctc ttgataaatg tgatggctta
     2161 gcggaaaacc aaaaaacatt atcaatagat gcatttttag gaaggtatct cttgcataaa
     2221 gatttaagat caacccaatg aagagcttta ttaccagtct cttcaattgc atgatataaa
     2281 ttatacccat caataaagca ctgcactcgc tttgttttta cttcgatttg ctcagtagtt
     2341 tgttgtggtt gctgctctgt ttccatactt gccatttttg acctctagat agcaaaaagg
     2401 cctgcatatg caggcctttt tgcgtctctt aaccgcagtt aacagaccac tgtatggacg
     2461 catccaatac aggaagaatt ttgtacaatg cgcttccaag cctcgacttg gatagccact
     2521 aagagcataa ctcttaggta gtggactcaa cttaagttct atataaaaaa agagcaaatg
     2581 gatgacattt atgtcatcct aaaagataca tgttactacc taactactct tatattaaga
     2641 gtagttaggt agtaactgtt cacattttga tttttttttc gcgagaaaag catctatgag
     2701 cacaaaaaac gcacagaaag aacaaaaaaa agagtcatta atcactactc actggggaga
     2761 tgatgcaacc atacatgggt ggacggctat accaaatagc ctccttatgc tgcaaggtga
     2821 tcttggtatt gggagtactg agatgtgtat tctgctgaat atattgatgc atcaatggcc
     2881 tgagtcaggg gagtcaatat cttttccgtc aattggtacc attgcatcaa gaataggtgt
     2941 atctaaaaga acaattcaac gcggggttag taacctagag gcattgggga tattaactag
     3001 ataccaatca acaaggaatg accctctaac caatggcgct aatatattcg actcaactcc
     3061 tctaaaagaa tatttaaaca aaaaagctaa aggcataaca attagcaaca gcaacaaaaa
     3121 gaaagaaaga aagaaaaccg gaattacacc caacataaaa ttaccacgca tttgcccaaa
     3181 atgtttgaaa actaaagcaa ccagccacga agaaattgtt aaattttttg gcgtgcgaaa
     3241 aacaatggct ggggaaataa taaattcata ttgcaaagaa tgccgagctt ctgaaaaaga
     3301 tctattttaa acagttgacc attttttata tggccaactg ccccccactc aagtaaatgg
     3361 gactgtttct aacgctccca tttaccataa cgtctcatta cgcgcaccgc taaaacgcgc
     3421 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
     3481 aaatgcgtca ggattgcttt cagctcactg catagctatg catgaaagtg aatggcgatc
     3541 ggtttgggga cttac
//
