LOCUS       CP090253                1308 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E305-2 plasmid punnamed5, complete sequence.
ACCESSION   CP090253
VERSION     CP090253.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394354
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Chen,J., Liu,C. and Zhang,R.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Chen,J., Liu,C. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:24:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,144
            Genes (coding)                    :: 4,944
            CDSs (with protein)               :: 4,944
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 73 of 200
            Pseudo Genes (incomplete)         :: 143 of 200
            Pseudo Genes (internal stop)      :: 35 of 200
            Pseudo Genes (multiple problems)  :: 42 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E305-2"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed5"
                     /geo_loc_name="China"
                     /collection_date="2018"
     gene            complement(join(1004..1308,1..232))
                     /locus_tag="LZT36_01275"
     CDS             complement(join(1004..1308,1..232))
                     /locus_tag="LZT36_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UIL83989.1"
                     /translation="MQTQNVVTDKEREIRKRDLEDKDRKNASERRQEQKNQNFTQVYPL
                     GWKRLRELFRKNPGAAELYSMLAENIDGSCGAVVADQTHLASLLGVGRQTISRYVKWLE
                     EQGVLVKIPVAGKVCAYALNPHEVWKGYDNAKPYAAFLTKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(56..232)
                     /name="Col(KPHS6)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
ORIGIN
        1 ccgcaccaca agagccatca atgttttctg caagcattga ataaagctct gctgcccccg
       61 gattcttcct gaaaagctct ctgagacgct tccagcctaa agggtatact tgggtgaagt
      121 tttggttttt ttgctcctgt cgacgctctg aggcgttttt acggtcttta tcttcaagat
      181 ctctttttct gatttcccgt tctttgtccg tcacaacatt ttgagtttgc atagactatc
      241 tctcctgcga caccatgtcg tttgaaagta acatacagtt gtcatttttt tgtgtcaaca
      301 tgtcgcattt gcacgacatg ttgggtatat ctgtcgcatc ctatagatgc gacaacgttg
      361 tcgcccctag gatgcgacaa cacatttata aacccataat aaaaacaaca acatacaatt
      421 ttttccttct tatctaaaaa gatctcacag cgagctgcga gatctccagg tagcaccctc
      481 ctttcagtcg ggcaaaaaac ggtgaaaatt taaccattgg caaccgaaaa atcgtggggg
      541 ccccttcagg ggttccgatt tttttgttgc caacgcaccg atcaaaaaaa attggggaag
      601 gtggttgcgt tcacgcagtg agcgcaacca cctgccagac gggttttagc gccgcaggcc
      661 gcacatgttt ctgagccaac ggcgaggaaa catataagtg cgcatctctc taatttcacg
      721 aatttgagag atgagatcag tctggataat cggagtactc cgcctctagc cgtcctggtg
      781 aaccagtccg gcaagaccca cgaaatcacc cgtaaaacgc aataaaacgc gttttgacgc
      841 gttttttgac gttctacggg ctcacgtggg ctttgggcgg aaaaatggct tacgtgcgtt
      901 ctgagagcgt tttagggctt tgcagggtgg agtggtgaag cagctccttg tggaacctgt
      961 accgcccttg cgggcgggct tcggcgattt ggcgaacggg aatctattcg gcttctggcg
     1021 tgtcgccttc catgccctga cgggcatcgt ttccgtggaa catggccatg attcggcgct
     1081 gaatatcgcc gtctttgttg actagggttt tcgtcaaaaa tgcggcatac ggtttggcat
     1141 tatcgtagcc tttccagact tcatgcgggt tgagagcata agcgcatacc tttccagcta
     1201 ctgggatttt tactaaaaca ccttgctctt ctagccactt aacgtaccta gatattgttt
     1261 gtctaccaac accaagcaac gatgctaaat gggtttgatc ggcaacaa
//
