LOCUS       CP090251                2096 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E305-2 plasmid punnamed6, complete sequence.
ACCESSION   CP090251
VERSION     CP090251.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394354
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Chen,J., Liu,C. and Zhang,R.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Chen,J., Liu,C. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:24:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,144
            Genes (coding)                    :: 4,944
            CDSs (with protein)               :: 4,944
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 73 of 200
            Pseudo Genes (incomplete)         :: 143 of 200
            Pseudo Genes (internal stop)      :: 35 of 200
            Pseudo Genes (multiple problems)  :: 42 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E305-2"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed6"
                     /geo_loc_name="China"
                     /collection_date="2018"
     gene            complement(934..1923)
                     /locus_tag="LZT36_00510"
     CDS             complement(934..1923)
                     /locus_tag="LZT36_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UIL83865.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKGG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          complement(851..932)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="90.0"
                     /evalue="3.4000000000000004e-27"
     Typing          1017..1208
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 aaccattttc cgacaaatcc cccctcaggc cttgcggcac aagggctgga ggcgaatttg
       61 aatcctagag ttatccctta agtataaata caaggatcgc gcgcgagatt tgcttgaatt
      121 ttgagcggga ttttcagtaa cccaaaacca cccaacccca cccacttaca cccatctcat
      181 ccacagactt tcaccccgat tttgtgaaca acaaaaaaac cgtcttacag gtggttggcc
      241 tcactgcgtg acaccaacca ccatccccag tgtttttccc gtgccttcgt caatcacaaa
      301 atcggaaccc acgccggagg cgcgggcccc cgcgattttc tgtttgacga atcagggaac
      361 ggtgaacggt acgcggaatt ttttcgcgta ccgtgaggcg ttggcggggt gtcggggcgc
      421 agtcctgacc aggttcgtag aaccgcacag ggttgcaaag gggtgtcccc tttggcacaa
      481 gcgacggagc gaaacgcccc gttttgtgta cgccgcttat cgtgcctgtg ttttaggaga
      541 acaaaaaaat gccgtgcata cagcccacac aatgacccac ataatcaccc acaactcaaa
      601 acacacagcc cacacaatca cccacacttt aacccacaaa aaagaccaca accccattga
      661 caccccacaa aacaacccac acaaacggcg agtaaatcat tttgccgttt tcgtgccgca
      721 gccgttccgc gcaatcgaca aactggcagt cagggaatct cacgaagtgc gcacttatac
      781 aaactcacct taggttcgtt tgtcgtgcgg tctgcgacgc tcaaaaaacc gagaaacggc
      841 gggaattttc gggacaggat gtgtaattgt aataccgtca cacgcgacgc tatcacaatt
      901 gccatctggt caggggtgca ccccgacacc ccgctagcct gattcagttt tatctttcgt
      961 cggtgagcga cggtactttc tgacctcggt tttccactcg aacgcgatgc gtgaaccgtc
     1021 atcgtcgccc tcggaaatat catcaccgat caccatgtcg gcgtcagttt cctgatcgag
     1081 tttcagcacg tctttgagtg cgccgccggt cgccacaaag cggcgcttgt gcgtctggcg
     1141 ggtcagttcg aggaaccatt caggatcggc caccatgtca gccggcttcg tggaatattt
     1201 cagggtttcg gccaccgcac cgcgcaccag ttcggcggtc gcgcaggcca gcgattcgcc
     1261 gcccttcggc ttacgaggtt tcactgcccg gacatcaaca ttcgggtcat aatcgacccg
     1321 cagacactcc cgccagagtt caacccagcg ttcatgcctg acgtaacttt tcccatttag
     1381 catgccaggc ggcaccatca tcagggtgtg gaagtgcggg tgtgcgctgc cgtcgctccc
     1441 gcgggtgact tccgtggtac gtatccagcc ctgcacaggc cggaactcct tgcgaacctt
     1501 catgcgctga aaagctgcat tcatccggtt cagcatctcg cccagctccc cgatcgcaca
     1561 gttgcgcacg gtcagagtca ggaacatcca gcgggcatcg ggataatccg ccacgatacg
     1621 cggcagggac tggtagaaac gggcttgcca catcagggag cgcctccact ggcagacagg
     1681 acaatgccgg acacggcaga aatgcgcctc acgcagcctc agacgcgttt ctccggtttc
     1741 cttgagggta gaccacccga aacgcagtaa accaccacag agggccatac gcgccccgta
     1801 gcgctcatat tcggctgcaa gcaggtaaat ccctcccacg tcatcagaaa cgcttctgtg
     1861 agcgtcccag ggcgcatctg agggggaata gtccgtgagg ttctggagat cagggttttt
     1921 catgcgcacc acccgatagt gggcgatggc tcatctgacg tgttatcatt gccatatcac
     1981 ctgttgtgtt agtcgtatca ccaattcgaa ttcctacagg tggtcacacc cccgaacgat
     2041 tgccctcgtt gcgggggtcg cttttaatgc gaccactccc gacagaataa ctccaa
//
