LOCUS       CP089864               10060 bp    DNA     circular BCT 22-DEC-2021
DEFINITION  Klebsiella pneumoniae strain KPN23 plasmid pKPN23_2, complete
            sequence.
ACCESSION   CP089864
VERSION     CP089864.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN24039392
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Kochan,T.J., Ozer,E.A. and Hauser,A.R.
  TITLE     Klebsiella bloodstream isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Kochan,T.J., Ozer,E.A. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2021) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 175x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/16/2021 10:02:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,399
            CDSs (total)                      :: 5,278
            Genes (coding)                    :: 5,135
            CDSs (with protein)               :: 5,135
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 70 of 143
            Pseudo Genes (incomplete)         :: 78 of 143
            Pseudo Genes (internal stop)      :: 34 of 143
            Pseudo Genes (multiple problems)  :: 30 of 143
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN23"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN23_2"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.87 N 87.63 W"
                     /collected_by="Fiorella Krapp"
     gene            join(8828..10060,1..453)
                     /locus_tag="LVJ63_26935"
     CDS             join(8828..10060,1..453)
                     /locus_tag="LVJ63_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UHL81616.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            463..720
                     /locus_tag="LVJ63_26940"
     CDS             463..720
                     /locus_tag="LVJ63_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="UHL81617.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            805..954
                     /locus_tag="LVJ63_26945"
     CDS             805..954
                     /locus_tag="LVJ63_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UHL81618.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2438..4402
                     /locus_tag="LVJ63_26950"
     CDS             2438..4402
                     /locus_tag="LVJ63_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UHL81619.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4402..5133
                     /locus_tag="LVJ63_26955"
     CDS             4402..5133
                     /locus_tag="LVJ63_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="UHL81620.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            5140..5670
                     /locus_tag="LVJ63_26960"
     CDS             5140..5670
                     /locus_tag="LVJ63_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UHL81608.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5697..5876)
                     /locus_tag="LVJ63_26965"
     CDS             complement(5697..5876)
                     /locus_tag="LVJ63_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UHL81609.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5902..6330)
                     /locus_tag="LVJ63_26970"
     CDS             complement(5902..6330)
                     /locus_tag="LVJ63_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UHL81610.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6494..6808)
                     /locus_tag="LVJ63_26975"
     CDS             complement(6494..6808)
                     /locus_tag="LVJ63_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UHL81611.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6796..7074)
                     /locus_tag="LVJ63_26980"
     CDS             complement(6796..7074)
                     /locus_tag="LVJ63_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="UHL81612.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7249..7614)
                     /locus_tag="LVJ63_26985"
     CDS             complement(7249..7614)
                     /locus_tag="LVJ63_26985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="UHL81613.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7611..7982)
                     /gene="tolA"
                     /locus_tag="LVJ63_26990"
     CDS             complement(7611..7982)
                     /gene="tolA"
                     /locus_tag="LVJ63_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="UHL81614.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8256..8501)
                     /locus_tag="LVJ63_26995"
     CDS             complement(8256..8501)
                     /locus_tag="LVJ63_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UHL81615.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             3828..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1058..1636)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4403..5130
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gagaaattaa aagcggctca ggcatcttta aacgcaatga atgaatcctt atccagagat
       61 aaggctgcac ttaccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat
      121 gcagaaaata agttaaatga ggaaaagaac aagcctcgca aggggactaa agactacggc
      181 catgattatc atccagtacc taaaacagaa gacataaagg gacttggaga gttaaagcga
      241 ggtgatccta aaacacctaa acaaggtggt ggtggtaagc gggcccgatg gtatggcgat
      301 aaaaaacgta aaatttatga atgggattcc cagcacggtg agcttgaagg gtatcgtgcc
      361 agtgatggcg aacacctagg cgcatttgat cccaaaacgg gtaagcaggt taaagggcca
      421 gatccaaaac ggaacattaa aaaatatctt tgagaggtaa atatggggct taaattacgt
      481 ttgacatggt ttgataagaa aactgaagag tttaaagggg aagagtattc taaagacttt
      541 ggtgacgatg gttctgtcat tgaaagcttg ggaatgcctt taaaggataa tattaataat
      601 ggtgagtttg atttgaagaa agaatgggtt cctttactac agccattttt taaaaatcaa
      661 ataaaacctg atgtggataa ttgttatata gcttttgatt atcgggacgg taactggtaa
      721 caaaaaatta tcaaggatga tttgaaatac tgacttccgg tgttcaggga tgaatgccgg
      781 agtctttaaa tacagggaaa gtatatgaaa aaggtaaaga ctatttttct ttttatattg
      841 attgttgctg gctttcttct ggccgcatgt caggcaaatt atatccggga tgttcagggc
      901 ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga
      961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa
     1021 aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt
     1081 ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt
     1141 ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aactcccagt
     1201 taccaatggc tgctgccagt ggcgttttgc gtgctttccc gggttggact caagtgaaca
     1261 gttaccggat aaggcgcagc agtcgggctg aacggggagt tcttgcttac agcccagctt
     1321 ggagcgaacg acctacaccg agccgagata ccagtgtgtg agctatgaga aagcgccaca
     1381 cttcccgcaa gggagaacgg cggacaggta tccggtaaac ggcagggtcg gaacaggaga
     1441 gcgcaagagg gagcgaccta ccggaaacgg tggggatctt taagtcctgt cgggtttcgc
     1501 ccgtactgtc agattcatgg ttgagcctca cggctcccac agatgcaccg aaaaagcgtc
     1561 tgtttatgtg aactctggca ggagggcgga gcctatggaa aaacgccacc ggcgcggccc
     1621 tgccgttttg cctcacatgt tagtcccctg cttatccacg gaatctgtgg gtaactttgt
     1681 atgtgtccgc agcgcccgcc gcagtctcac gcccggagcg tagcgaccga gtgagcgagg
     1741 aggcgcaatt tccccggcac ctgcaagacg gtcaccggac gcgctgatgt gggatattgt
     1801 cccctgatta agtctttcta ttccctgacg atcaattatc aggataggtg aaccatgtcg
     1861 gtttttcgct gaacatttta cgttaaccta tctctgttgc ttttttctcc ttgcggagaa
     1921 atgcaagtca tccccgctgg ggcgtggctg acttcagggg aaaagacctt gcttacgctg
     1981 gctggacgcc accgacgcac ggtcttttcc cctctttctg gctctgtccg gctggacgcc
     2041 ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc cacaaaaatc
     2101 cacggtgctg gacgcacctg acgaagcagc aaagctgctc ctgattttgc gggtggtcag
     2161 ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg cactggggtg
     2221 ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct gcgccttttc
     2281 cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct gaacgcaatc accacggacg
     2341 tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg gctgcgcatc
     2401 acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg attcgcttgc
     2461 ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca gctggctgta
     2521 ccgggagtat tacagtctgg ccggtttttt tctggttatc ggcctgtttt tcggtctggc
     2581 tgaactctgg gccggccgaa tgcgttttgt ggggctgagc cataaggcgc ggcgggagta
     2641 tgccgatcgc tataagcgcg ataataaccg gactgtgcca ttttcggtgc tggcctcgct
     2701 ggattcaccc gggcggatgg tcagggcacg gcgacatgtc tggaactacg cgtactggct
     2761 gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt ttatcgagag
     2821 ctggggagaa accgtcctgc gcccggacct ctatgcgaac gcagattatt acctgctgca
     2881 gccggctaac ctgttcggga tagcctggtg tgccggaggg cttgtcgccg gcgtccttct
     2941 ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt gcggccgctg acgaggcggt
     3001 gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggaccggag aaatgacaga
     3061 cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg atttttccac
     3121 ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag gatgagcagg gccagccggt
     3181 gctggtccct cgtgatacct ggcgcaagac caatattcag atcctcggcc tgcccgggag
     3241 cggcaagagc gcgatggcca ccaacgcgct cattcgctgc gtacgcgatt tcggggacgc
     3301 tgtggtgtac ttcgacccca aaggggacgc gtgggcgccg cacgtcttcc gggcgcactg
     3361 cccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga atctgttccg
     3421 cgatctcgac cagtacgccc tgaagaatct gctggttgcc gggtttaacc tgagtgagac
     3481 cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc tgattgcgga
     3541 gcagttcccg cagggggcaa acattcagca ggtgctggcg gcggcgtacg cgttgccgga
     3601 ggccctgaaa aaggacgtga aggggctgat caccaagctg gagaacgtgg ccgacctgag
     3661 cgtcctgcag acggacagcg gtatcgatgt ggcggggata atcaacggcg gcggctgcct
     3721 gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg gtgcagaaga tgctgtttgc
     3781 ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg aggtggccgc acgcgagcat
     3841 catgctcgat gagattaaat atctgctgtc gaagtacgtg ctgaacgcgc tggggacgct
     3901 gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg ctgggcgact tcggtcagtg
     3961 cgggcaggat ctgcccgctg attttgtgaa aaccacggta ctggataaca cgccgatccg
     4021 ctggttctat cgcgcggcca gccaggagtc cgcgcagtgg gcggcgggcc agaccgggga
     4081 gatccgggtg gacgtggagc gccgccgggc cagccgggag gcggggaacg tggagcacat
     4141 cagcggcgac agcttcatcc agaaggaggc gcggccgctg tttgacgtga acaccctgca
     4201 acacctgcct gacgggttcg cggtgatgac cgggctgggc gtggcgcggc tggcgttcag
     4261 cagcccgctg cgggttgagc gccgggaaat ttcgctgaaa accttcccgg tgctggcgaa
     4321 aacggacccg ctggcggagt acctgcctcc ggccgacggc ggcggaccaa aagcggatga
     4381 tgattttgcg gggctttact gatgacactg gagcagtaca acgtcagcca cgcgaagcgg
     4441 caggcgcgga acgcggagaa aacacggctg accctgcgct ggctgcggga agagctgtgt
     4501 tccacggcgg agctggttgc gcgacggctg gggattgcgg cggtacagcc ggtttaccgc
     4561 ttcctggaca gtctggtggc gaaagggctg ctggtccggg cgaaataccc ggttgacggc
     4621 cgtcaggtca gcgtgtgggg gctgacgccg cacggggtgg cgttctcgtt cgacgaggac
     4681 gagccgctga cggacgttat tccgtttcag ccgtcaaggg tgtcggcggc gcagctgccg
     4741 caccggctgg ccgtgcagtc cctgcgcctc tccatggagg gccggggggc caccggctgg
     4801 cggtacctgc accgtatggc cctgaaaggg atgaaagtgc cggacgcgct ggcggagctg
     4861 gacggcagaa cggtggcatt tgaggtagag cgcaccgtca agagccgcag gcggtatcag
     4921 gaagtggtgt ccgggtatct gttcaaccgc cgggcgaacg gcatcgatga aatctggtac
     4981 atctgccctg accggccgac gcttgtccgg gtacagcggg cgattctggc ggtggatgaa
     5041 atcgtgaatc cacagaccgg cgaggcccgc aaaacggtgg agctggaccg ggagaggctg
     5101 ttcgcgtgct ttaaatttat gacaacggag tgagggatta tggcgctgta catggtattt
     5161 attgaggacg tggaagacaa agacggcgag cgctgcatta tgccaaccag cgagcatctg
     5221 gaagacatgg tggtgctcgc ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg
     5281 gcccagcatc tggcgatgat gaaatccatc gggatgtatg acgagcggct caacagcgac
     5341 gaaaaagcca cgtacttttt caccggtgaa aatccgcaat gggaacggga caaacccaca
     5401 ggaatgaagc tgtgtgatta cctctatcga aaggtggagc ggttcgccat cgagaacggt
     5461 gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga ttaacgcgat gattgaggtg
     5521 gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg
     5581 gaatataaca gccgctattt taacgtgccg gtgcctgaac tgccggagga caaacccgcc
     5641 cgccggtggt ggccgttcgg caaaaaataa cgccagcacc ggcatggccg gtgctgttat
     5701 tcttccccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc gtgaagccgt
     5761 tcgcacatgt cggccggggt atccatgtcc agctcgttca gcttttccag caacagcagc
     5821 gactgatcct taatgaattt agccatattg agggcagttt tttcctgctt cgtcatgttc
     5881 tccattccct tcggtaagtt ctcagctaaa ccagccggac cagaagcgtt ttttcttctg
     5941 tgtcagttct gttgtcaatc tgtccacttc ggcctggagt ttccggcgct catcatcgtg
     6001 ctggcggcgt tcttcttccc gggatgtttt atcttccagc atcagggtga cagcctgccg
     6061 gagatcggcc agttcctttt gcattgccag aagctgttcc tgtgggaaat ttttttgtgt
     6121 attttcacgg tgtgaaatgc cgggaatttg tttctcaggg atattcacat ttccgtatac
     6181 acggatcagt tctgacacat ccacgacagg gttgtttttc ccgtcacgtg acacggtaac
     6241 ctttccctgt tttacatgat tgtatagcgt tcttctggta atgcccgcgg cttgtgctgc
     6301 ctgtgaaagg ttgagcaatg ttttcgccac tgtatacccc tgattttatc gggtgtgaaa
     6361 ggtgtgtaat ttctcacggt aaatgtatcg ctgaatttta cccgtttttc cgttcaggat
     6421 gtggttttct gacgcgctgc gcttgtcaaa ccggcgacgg ccacgcaatg aattgcgccc
     6481 ccgtcatcgg ttattagagg ctgtctgccc ccggataccc ggattctttc tgatgcctga
     6541 cggtgacgat cacaatctgg tccatttccc ggtcgtggcg gtagagcatc acatagccac
     6601 tgtcgccaaa cccgatcacc agttccttat attccagcgg cagaaacggc accgggcggc
     6661 caatgtctgg caatgttttc agttgctgga tagcccggac gatcacctca cctccttttc
     6721 tggctgccag ccgatttttg gttttgagaa agtcctgaag gcgttgtaaa tcgcgctgtg
     6781 ccagcgcgga aatcgttacc tgtggcatgg cggagcatcc tgctcgttct cagttcccca
     6841 ggtacttatc caggcttccg cttcttccgc agtaaggtgc agacccgttt cctgatagtg
     6901 ctgccatgcg gcctgtccgt cccgcaggta ctggctgcgc ttctcttccc ggtcgatgta
     6961 ttcggtgatt gcctccagca ttagcgcgtg cgctgagcgg tgccggtcat cggccagcgt
     7021 tttcagccgg tccttaagtt cttcatcgag cctgatggat gttgctgatg acataaaagc
     7081 ccctttgtag tcatatgtat tacacatgac tactttaatc cgtggcggcg ctatttgcca
     7141 gaaatacggc ttttgctgcc catttttcag cttcgcttcc actaatcagg gctgctacag
     7201 gctcactcat catccaggac tattgcagcc tgcgggttaa aggacctgtt actggcggca
     7261 accatcatca ttgagctttt gggtggtgac tggtcgcgga tcctgtaagc cgtcattttt
     7321 cagactgaat tcaaaattta taacctgtcg cattgccggg tgccctgcgg gatatagcca
     7381 gtgcctcatt gccgtcagcg tttcccgaaa aaaaaggtga cagccggtaa gcgacacgtt
     7441 gtccactcgt ccatcgtcca gaatatcaaa cgtggcggtc acctgacctt cgatgcgttt
     7501 tgctgcggcc tgtaccgggt aggttggctg cagtgtggtt acgggtttta gtggtatcgc
     7561 atgtaccgga gtgaggaaca tggccgccag cactgtcaga gtaaaagctt tcatggtctg
     7621 aaatccagag gggcgttttt gaatatttgc caggtatttt catccggtgc cggcggaatt
     7681 ttggcgtggg taacggctga tattgctgct ttacagagtt tcggatctcc tccttcagca
     7741 atggcgctgt tcagagtgcc atcacgttgt atggatatcc gcacggtgca catttttccc
     7801 gcataggcct ctggttcaaa catctgcatt tcaacagcgg tcttgatggc cgcagcgtag
     7861 ctactgattt tagatgactt atccagagta taaattttcg gcggctgggt ggtggaatgc
     7921 ttacaggccg tcaggagtaa ggggctgcac aatgccagca ggataaaggg tattaaacgc
     7981 atcgggttga tgacctgtca gtaaaaaatg ttacatgtat atttgtcact ctttacggcc
     8041 acgttcattt acataaacac ggcttatcgt atcgttttta caccaaacgg cccgccatgc
     8101 agttgcgtgc ataggcatgc agcgggttac aatttaccgg gcgcgtttcc ggcggttttc
     8161 cgggtggttt gttgcctgtt ttatcccgta gccgccggaa acgccctcag accgtctgag
     8221 cggtgcgggc aatgcgacgt tatggtaaat accccttacc ggcggcgctg tggctgccag
     8281 gtgaaggtgg tttccgtagc ctcgtatttc tcccgacgca tttcctgcgc ccgcctggct
     8341 tcacgttcgt cctgaatggc acgaagcacc ctttcccgca cctgttgttt taccgccttt
     8401 ggcagcgttt tgttcccttt caggcccatg gccagtgcct gagcgacaaa ggcgtccgcc
     8461 aggcgctttt cttcagcggg agtcagtttc aggtgcgtca tagtggcctc cgggcgtctc
     8521 aggtttcccg aacagtttaa ccgcaggtcc tctcctcatg ccagttagct ttcagatgtg
     8581 tctataccat ctgaaatgta agtgtgatga atgtcttaaa taataaaatt tgttgccatg
     8641 agaaattttc gggaaaagac gcgatctgct tacaggtggc tccgggcgct tttttgtgcc
     8701 tgaagatgag aactaatctc atatattgac acctgaaaac gggaggagta aggtaataat
     8761 tatactgtgt atatgtacag tatgttggct ttaatttatt gttttaaatg tcaaagagga
     8821 aaacggtatg agtggtggag acggtcgagg tccgggtaat tcaggtctgg gacataatgg
     8881 tggtcaggcc agtgggaatg tgaacggtac gtctggtaaa ggtggccctt catcaggtgg
     8941 tggtacggat ccaaacagcg ggccgggctg gggtacgacg catacgccga atggagatat
     9001 tcataactat aatccggggg agtttggtaa cggcgggagt aaacccggtg gtaatggtgg
     9061 taacagcggc aatcatagcg gtagctctgg tggcggacag tcttcggcca ctgcgatggc
     9121 cttcggtctg cctgccctgg ctactccggg ggctgaagga ctggctttat cagtttccgg
     9181 cgatgcgttg tcggccgccg ttgctgatgt gctggctgcc ctgaaggggc cgtttaagtt
     9241 tggtctgtgg gggattgcga tctacggtgt gctgccttct gagattgcaa aagatgatcc
     9301 gaaaatgatg tcaaaaatta tgacgtcatt accggccgat gcggtgacgg agactccggc
     9361 aagtacttta ccactggacc aggcgacggt tcgtgtcaga caacgggttg tggatgtggt
     9421 gaaggatgag cggcagcata ttgcggttgt cgcaggtcgg ccaatgagtg tccctgtggt
     9481 ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg gtctcccgtc
     9541 tctgcaggtg agcgtaccga aaggtgttcc gacagcgaaa gccccgccaa aaggcattgt
     9601 tgctgaaaaa ggtgattcac gtccggctgg ttttacagcc ggtggtaact cccgtgaggc
     9661 cgttattcgt ttcccgaaag agaccggaca gaagccggtt tatgtgtcgg tgacagatgt
     9721 tcttaccccg gcacaggtaa aacagcgtca ggaggaagaa aagcgtcgcc agcaggcatg
     9781 ggacgccgct catccggaag aggggctgaa aagagactat gataaagcga aagctgagct
     9841 ggatgccgaa gataaaaata ttgcgacctt aaacagccgc attgcatcga cagagaaggc
     9901 gctccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag aggcagaggc
     9961 aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg ggtggcagga
    10021 tcaggttcgc tatcttgata aggatattca gaatcagaat
//
