LOCUS       CP088878                5165 bp    DNA     circular BCT 05-JAN-2022
DEFINITION  Escherichia coli strain F17EC0245 plasmid pF17EC0245-4, complete
            sequence.
ACCESSION   CP088878
VERSION     CP088878.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391709
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of medicine, Gangnam Severance Hospital,
            Yonsei, Seoul, Select One 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/03/2021 09:36:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,180
            CDSs (total)                      :: 5,056
            Genes (coding)                    :: 4,814
            CDSs (with protein)               :: 4,814
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 107 of 242
            Pseudo Genes (incomplete)         :: 156 of 242
            Pseudo Genes (internal stop)      :: 40 of 242
            Pseudo Genes (multiple problems)  :: 54 of 242
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F17EC0245"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF17EC0245-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(4854..5165,1..87)
                     /locus_tag="LQQ71_25880"
     CDS             join(4854..5165,1..87)
                     /locus_tag="LQQ71_25880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIB47930.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(291..1205)
                     /locus_tag="LQQ71_25885"
     CDS             complement(291..1205)
                     /locus_tag="LQQ71_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UIB47931.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            2940..3149
                     /locus_tag="LQQ71_25890"
     CDS             2940..3149
                     /locus_tag="LQQ71_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIB47927.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(3215..3748)
                     /locus_tag="LQQ71_25895"
     CDS             complement(3215..3748)
                     /locus_tag="LQQ71_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIB47928.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3751..4677)
                     /locus_tag="LQQ71_25900"
     CDS             complement(3751..4677)
                     /locus_tag="LQQ71_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UIB47929.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     FTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(292..1205)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.802"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3755..4677)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagctatgct ataaaaaaga caggatatta caagcactta aggaacttaa gtacgatctg
       61 gagacatccc caaacccaga cgcgtaagtg cctccatccg tgttccgatt ccctggtgct
      121 tcctcgctca ttcgctcgct acgctcggtc atgagactgc ggcgagcact accaccacaa
      181 tcacgcggaa agccgcgcca ttgcagggct gaacggggaa aaacggcaaa aaacctgctg
      241 ctcaaaaaga cgccagaaag gcgctgttat ctgatatagg cttatttggt ctactctacg
      301 agacctcgtt ttttcagttg gtaataccag gcgcgactga tccctaatgc ctcccacggt
      361 ttcagcgtgc gtgcagatgt agctactgtg ctgcgcttag acttagaacc gcccttgcga
      421 ccgcgtgcag cctgaatttc tggcgtgtgc gtatcagcca catactgtgc gaaagtttcc
      481 ggcgtgaagt tcctgtgcgt gtatttcgca atgctcttgc caatagcccg acattcagcc
      541 agtgaaagcg gaacgggaag cgatgcattg tacatttcga cacgctggat caccgcttca
      601 agccactgtg agaacgctgg ccagccctga cgaatcgccc tgtaagccca tttgcgcgtc
      661 atttcgaaca ggtggcagtt tcgccccatc ccgtaatgtt tatcgatgct acgacgctct
      721 gaggcgctca aatcgagata atcagccagt tcatcgaggg tataggcttc ctcgcgccac
      781 tccatcacca gccattccag gtggaacgga tttttgcaaa tcaggccgct gtagttcaca
      841 tccgcacaaa gtttctcaca cagcgaacgt tcaaccgctg cggcatattt cagcgcctta
      901 accgaggcat caggcgcggt tctcacggcg atgttgagtg cgtagagcag gtgagcgtgt
      961 ccgttcacag gatttttaac ggtgatattc ggcgcgggag cgttccggtc gctccagtca
     1021 atcgcagccc cctcgcggtc aacgtcaaaa accagccaga actgtgcatg aggctggttc
     1081 tgctggatat accgtgcaag aagcgcgcgc cctttgccgg agatacgaag gccgaaagcg
     1141 agatcatccg tgtgataggg acggtggggt aaattttctt cgaagtattg aagcgcggcg
     1201 ctcatgtgtt acgattcctt ccaggttatc gaccgacatc gcccctgtat tttcgtgaaa
     1261 gtcttggcgg atagacacga atttcagggg cgtttgtttt tcagtcgctt acactacacc
     1321 caacggcaaa tcctgtcgat ccttcccgaa aaaccgatcc atttaccata actcccgttg
     1381 tacgtaccat gtaaaacgcc atcaatcgct ttctggcgca aaaaacgcaa aaagacaaga
     1441 aaacaccagg ccaaaccaga aaaacgcgtc agaatcgttt tcagggcgtt gcataactat
     1501 gcacgaaagc gatgtgcgat cggtttcgtg cgttctgaac accgaacggc tcaaaacgac
     1561 aagttttctc ccctggtgcg tggcgctctg acggggtttc ctgtttccaa acttcacatc
     1621 tcgccaggac aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa
     1681 tattttagtt atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta
     1741 aaacaacctt tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca
     1801 caaaagtggg gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga
     1861 tgaagtagct acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt
     1921 aagatcctga cgaaggattt ataaatgcac gaaaacacac agacagacaa attcactaaa
     1981 ttcatacaca aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt
     2041 atccacatcg cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa
     2101 aaaataaaac cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac
     2161 aatatcacaa aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta
     2221 taaaagcgac tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac
     2281 tgagcatcca tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact
     2341 taaagattag cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc
     2401 agtactatcc atacagctaa ttacatataa atactattaa accatctgga cattaaaaat
     2461 aaactcttat aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa
     2521 atggaggtat cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc
     2581 gagcgcagag ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat
     2641 acagaacaag aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct
     2701 taaagctcac ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt
     2761 acggcagact tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg
     2821 aagttatttc tccttacata tccccctatg ccaacggggg attttttctg gttcactaac
     2881 gcataaataa tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca
     2941 tggcaaaatt aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat
     3001 caagccaggc atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag
     3061 ctcttactga tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt
     3121 atcgaccaaa gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaatccttt
     3181 cgcgggtcgt gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga
     3241 caggtttctc caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg
     3301 cgttgtagtt cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca
     3361 gaaaagccac tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt
     3421 cctgcacgat attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct
     3481 tcatgtttgc gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca
     3541 gtcctgccac gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca
     3601 caccgtcaga aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag
     3661 caacagcttt ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg
     3721 tgagtaattt ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg
     3781 tgatttaacc cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa
     3841 gccatgctcc cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg
     3901 agtgtccagc cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga
     3961 atagtccggt tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc
     4021 ggcagcttca aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt
     4081 ccggcagtca atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac
     4141 ctcccagcgt tttcgcaatt cccgcaactg ctctttttgc tgagtgaaag tcaggcttcc
     4201 ggaatccttt ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg
     4261 ttccggtgta cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg
     4321 ctgttcgccg ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg
     4381 ttcgccagct tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag
     4441 ttcacggggt aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg
     4501 gtcagccgcc tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg
     4561 tgcggaatat tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc
     4621 gtggtttgca gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt
     4681 ttcagccctc tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct
     4741 tccttctaat tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga
     4801 tatactgcac gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca
     4861 tcagcaacat cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga
     4921 ccgcacgaca gagacagatt attgacctga tcgacaacga agccggactc acagaacagg
     4981 aacgcaaact tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga
     5041 gcgaacgaaa acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca
     5101 atcaccggct ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa
     5161 caggc
//
