LOCUS       CP088877               10119 bp    DNA     circular BCT 05-JAN-2022
DEFINITION  Escherichia coli strain F17EC0245 plasmid pF17EC0245-3, complete
            sequence.
ACCESSION   CP088877
VERSION     CP088877.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391709
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of medicine, Gangnam Severance Hospital,
            Yonsei, Seoul, Select One 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/03/2021 09:36:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,180
            CDSs (total)                      :: 5,056
            Genes (coding)                    :: 4,814
            CDSs (with protein)               :: 4,814
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 107 of 242
            Pseudo Genes (incomplete)         :: 156 of 242
            Pseudo Genes (internal stop)      :: 40 of 242
            Pseudo Genes (multiple problems)  :: 54 of 242
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F17EC0245"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF17EC0245-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            200..424
                     /locus_tag="LQQ71_25830"
     CDS             200..424
                     /locus_tag="LQQ71_25830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIB47921.1"
                     /translation="MNADQVCAILAWRNAEREKLEAEKERDSIIDLKTTLKEALAERDN
                     MPPTLDELGAELKAFVESSFDELNEVMQL"
                     /name="hypothetical protein"
     gene            648..1364
                     /locus_tag="LQQ71_25835"
     CDS             648..1364
                     /locus_tag="LQQ71_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIB47922.1"
                     /translation="MQLDEHSRKIAALVAYNEKQIKEINRIITLLNERVNDLNTFTDTL
                     EREGKVVSGISYSLRDSVSHVDFYLNKGMEEVSCKLQKSVSDTLTSLLREKLSFTLNDV
                     RQEWTDFEEQTSKEIKESLKQTTRDAEVATGIIRKTMEKYTVTRIVEKVLVLLVLFLLL
                     MIAPSFYAEYLKMNIGSLMEEQKKHESMLKEWKEKAPLAEFSKCGEYRDVCVLVDDKSG
                     YYSNEQTPNKKYAILK"
                     /name="hypothetical protein"
     gene            complement(1422..1994)
                     /locus_tag="LQQ71_25840"
     CDS             complement(1422..1994)
                     /locus_tag="LQQ71_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="UIB47923.1"
                     /translation="MEYSTLFSFAIVTLSQTISIGPGVALVINNAFSQGLKSSIKTSIY
                     IRAGEAIVMVISLFTLSSTSSIEQHFYIIKILGGSYLIYIGVMGLINKKNKKSKVQKPL
                     LIPLLNPKAYLFFAALIPAFIDDNTNIALNFFILGVLFIFISFLTDIIYIAISLTIRDK
                     LTPSFSRYISICSSIFILGTGIYFILT"
                     /name="LysE family translocator"
     gene            2594..3613
                     /locus_tag="LQQ71_25845"
     CDS             2594..3613
                     /locus_tag="LQQ71_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UIB47924.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARFIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKF"
                     /name="replication initiation protein"
     gene            complement(3874..4320)
                     /locus_tag="LQQ71_25850"
     CDS             complement(3874..4320)
                     /locus_tag="LQQ71_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="UIB47925.1"
                     /translation="MTNREDWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVARLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWERRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            4492..5586
                     /locus_tag="LQQ71_25855"
     CDS             4492..5586
                     /locus_tag="LQQ71_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UIB47926.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKARF
                     GETQQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGPVRVA
                     VMNADQVCAILAWRNAEREKLEAEKERDSIIDLKTTLKEALAERDNMPPTLDELGAELK
                     AFVESSFDELNEVMQLNSLIDGALDTMMKQFQVEEERKLQQERLIQAARDEAEKQRELE
                     RERVRRRAYSSPAPSRSRDYGMSR"
                     /name="MobA/MobL family protein"
     gene            5608..6324
                     /locus_tag="LQQ71_25860"
     CDS             5608..6324
                     /locus_tag="LQQ71_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIB47918.1"
                     /translation="MQLDEHSRKIAALVAYNEKQIKEINRIITLLNERVNDLNTFTDTL
                     EREGKVVSGISYSLRDSVSHVDFYLNKGMEEVSCKLQKSVSDTLTSLLREKLSFTLNDV
                     RQEWTDFEEQTSKEIKESLKQTTRDAEVATGIIRKTMEKYTVTRIVEKVLVLLVLFLLL
                     MIAPSFYAEYLKMNIGSLMEEQKKHESMLKEWKEKAPLAEFSKCGEYRDVCVLVDDKSG
                     YYSNEQTPNKKYAILK"
                     /name="hypothetical protein"
     gene            complement(6382..6954)
                     /locus_tag="LQQ71_25865"
     CDS             complement(6382..6954)
                     /locus_tag="LQQ71_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="UIB47919.1"
                     /translation="MEYSTLFSFAIVTLSQTISIGPGVALVINNAFSQGLKSSIKTSIY
                     IRAGEAIVMVISLFTLSSTSSIEQHFYIIKILGGSYLIYIGVMGLINKKNKKSKVQKPL
                     LIPLLNPKAYLFFAALIPAFIDDNTNIALNFFILGVLFIFISFLTDIIYIAISLTIRDK
                     LTPSFSRYISICSSIFILGTGIYFILT"
                     /name="LysE family translocator"
     gene            7555..8574
                     /locus_tag="LQQ71_25870"
     CDS             7555..8574
                     /locus_tag="LQQ71_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UIB47920.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARFIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKF"
                     /name="replication initiation protein"
     gene            9456..10082
                     /locus_tag="LQQ71_25875"
                     /pseudo=""
     CDS             9456..10082
                     /locus_tag="LQQ71_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     Typing          2595..3369
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.969"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          7556..8330
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.969"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          4493..5583
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.055"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaaggca cgctttggcg agacacaaac aagagcgaaa atcagccctg gttgcgctac
       61 gctctcactg ggctgatgtg cagaatgaac accttgcgcg acacggccac cagttcgcgg
      121 cgttgatcat cgttcgctga aagaacaggg tatcaagcgt acgccggagg tgcatcttgg
      181 tcccgttcgt gttgctgtta tgaacgctga tcaggtttgt gcaattctgg catggagaaa
      241 tgcggaacgg gagaagctgg aggccgagaa ggaacgggac agcatcattg accttaaaac
      301 gaccttgaag gaagcgttag ctgaacggga taacatgcca ccaaccctgg atgagcttgg
      361 ggcagaattg aaagcgtttg ttgaaagctc gtttgatgaa ctcaatgagg taatgcagct
      421 ttaattcatt gattgacggt gcgctggaca caatgatgaa gcaattccag tggaagagga
      481 gcggaagttt gcaacaggaa cgactaattc aggctgcgcg agatgaagcc gaaaagcaaa
      541 gagagctgga gagggaaaga gtgcgccggc gtgcgtattc ttcaccagca ccatcacgtt
      601 cacgcgatta tggtatgagc cgttaagcac tggaggtttt ttattttatg caattggatg
      661 aacattcaag aaaaattgct gcccttgttg cgtataatga aaagcaaata aaagaaataa
      721 acaggataat tactctgctt aatgaaagag tgaatgactt aaataccttt acagatactc
      781 ttgaacgtga aggtaaagtt gtatctggaa tttcttattc cttgagggat agtgtgtctc
      841 atgttgattt ttatcttaat aaaggaatgg aagaagtatc ctgtaaatta cagaaatctg
      901 tctcagacac actaacatca ttactccgtg aaaaattatc tttcacactt aatgatgtaa
      961 ggcaagagtg gactgatttt gaagagcaaa caagtaaaga gataaaggaa tcattaaagc
     1021 agacgaccag agatgctgaa gttgcgacag gtataattag aaaaacaatg gagaagtata
     1081 cagtaacaag gatagtcgaa aaggtacttg tgttgcttgt attattttta ttgttaatga
     1141 ttgctcctag tttttatgct gaatatctca aaatgaatat cggttctcta atggaagaac
     1201 agaaaaaaca tgaatctatg ttaaaagagt ggaaggaaaa agcaccattg gcagagttct
     1261 ctaagtgtgg agagtatagg gacgtatgcg tgttagttga tgataaaagc ggatattatt
     1321 ccaatgaaca aaccccaaat aaaaaatatg ccatactgaa atagtgttaa gtaactcatg
     1381 ccgtcttatg actaaggctg catgagtata aaaacggcgt ttcacgtcaa gataaaataa
     1441 atacctgtac ctaatataaa aatggagcta catatactta tgtaccttga gaatgacgga
     1501 gttaatttat cccttatcgt caagctaata gctatgtaaa taatatctgt caggaatgat
     1561 ataaatataa acaaaacacc caatataaag aaatttaatg cgatatttgt attatcatct
     1621 ataaatgctg gtattaatgc cgcaaagaat aaatacgcct ttgggtttaa taatggtata
     1681 agtaaaggtt tttgtacttt gctttttttg ttttttttat taattaaacc cattacccct
     1741 atgtatatta aatagcttcc acctaagatc tttataatgt aaaagtgttg ttctatgctg
     1801 gatgttgaag ataacgtgaa aagagatatt accatgacga ttgcttcgcc agctcttatg
     1861 tatatacttg tttttattga tgatttaagt ccttgtgaaa aggcgttgtt tataaccaat
     1921 gcaacaccag gtcctattga tatagtttga gataaagtta cgatagcaaa tgaaaataat
     1981 gttgaatatt ccatatttac ttaccatgat atatttcgcg ggagcttttc atatacatat
     2041 tccataggaa ttgcggcttt cggtaataaa tttataaatg tggatatgtg ctttagtgaa
     2101 ttgctggcgg cgattgagta aaaatttccg ctattccaat atattgtcga tatttcgttg
     2161 tccagactgt atatgtttgg aaatttgttt gcatacgggt tcgttccgtt tagctggctt
     2221 tttggatgag aaagtttgct gttttgaccc gttcgcgctt ctgaaggggg gaaaccgatc
     2281 atgaatcgct tttgtgcata gttatgcagg cccataaaaa cgattctgac gcgtttttat
     2341 ggtttaccct agtgttttct tgtctttttg agttttttgc gtcagaaagc gattgagagc
     2401 gttttagagg gtacgtacaa caggagttat ggtaaatgga tcgggtttgc gggaagactc
     2461 gacaggtttt gtcgttgggg gtagtgtaag cgactgaaaa acaaacgccc ctgaaattcg
     2521 tgcctctccg ccaagattgt cacgaagatt caaggggcgt gtgggtcgta acacacggag
     2581 ggaatcctaa cacatgagcg ccgcgcttca atacttcgaa gaaaatttac cccaccgtcc
     2641 ctatcacacg gatgatctcg ctttcggcct tcgcatctcc ggcaaagggc gtgcgcttct
     2701 tgcgcggttc atccagcaga accagcctca tgcgcagttc tggctggttt ttgacgttga
     2761 ccgcgagggg gcagcgattg actggagcga ccggaacgcc cccgcgccga acatcaccgt
     2821 taaaaatcct gtgaacggac acgctcacct gctctatgca ctcaatatcg ccgtgagaac
     2881 cgcgcctgat gcgtcggtta aggcgctgaa atatgccgca gcggttgaac gttcgctgtg
     2941 tgagaaactt tgtgctgatg tgaactacag cggactgatt tgcaaaaatc cgttccacct
     3001 ggaatggcag gtgatggagt ggcgcgagga agcctatacc ctcgatgaac tggctgatta
     3061 tctcgatttg agcgcctcag cgcgtcgtag catcgataaa cattacggga tggggcgaaa
     3121 ctgccacctg ttcgaaatga cgcgcaaatg ggcttacagg gcgattcgtc agggctggcc
     3181 agcattctca cagtggcttg atgccgtgat tcagcgtgtc gaaatgtaca acgcatcgct
     3241 tcccgttccg ctttctccgc ctgaatgtcg ggctattggc aagagcattg cgaaatacac
     3301 gcacaggaac ttcacgccgg aaactttcgc acagtatgtg gctgatacgc acacgccaga
     3361 aattcaggca gcacgcggtc gcaagggcgg gaaggccaat tcgtcagaaa atcagtcgga
     3421 caaagggaaa aaaagcgccg ctgttcggtg gactgctaac gacgacaaac gtcgtagggc
     3481 gcttgatatg tatatcttgg gagcgtcaac ggaagatatt gcagtggcgg ttggtgttag
     3541 cagccggact attcgacgct ggatggataa ttctggagaa tggttaacca agaaacaaat
     3601 aattaaattt taattaacag aaaaatgaca attaccccct gtttttgtta atgggtggtt
     3661 aaattcgcgg acatgaacaa gcctatatca gataacagcg cctttcggcg tctttttgag
     3721 cagcgggctt tccggcggtt ttctcctgtc agcccagcaa tggtgcggct taccgcatga
     3781 ttgaggtggt tgtgctcgcc gcagtctcat gaccgagcgt agcgagcgaa tgagcgagga
     3841 agcaccaggg aaacggaacg tgaacggagg ctcttacgca ctgtctttgg ttagtagctc
     3901 cttcccgcgt ctttcccatt cctgccattt tgggtggttg cgcggtagct ctgcgattcc
     3961 tgcgagtgcg cctaccagtt cggcagcact aaactttggt tttccggttt tgctgtccac
     4021 cagtcgtgcc acaatcatga ggcctgcggc cttgtagagt tcatgatctc gtgcctttcg
     4081 ttccgccctt gcggctgcct tccgttctgc cgctatgatt gccgttactc tggcttttgc
     4141 tgcggctgct ttctctgcgg ttttttctgc ccgtatcagt gcggacaacg ttttttcatc
     4201 cgtcgttgtt cggttttttt tctctgccag taacacaagt aatctctgct gctctgacgg
     4261 tgatttcagt ccgttgatgt aggcaatttt tgcacttaac cagtcttcac gatttgtcat
     4321 ccttacctct gcacttttgc cgttaatgtg agttgttatt gtgcataata tcaccttgtc
     4381 tgcctgcgtg ttagaccgta aaatcatccc tgattaattc agggatgcgc acttagacgt
     4441 ttctctcctt tgtcgagaaa cagtgcgctc ctgcggaggg cttaacggca gatggcaagc
     4501 taccattgca cagttaaaac tggagcaaaa ggcgtagcct taaaacatgc tgattacatt
     4561 tccagatccg gcgaatataa aaactataaa tcgcgggagg atctggaatt ttcttcatcc
     4621 ggtaacatgc catcatgggc gaagaaaaat cccgcagaac tgtggaaagc tgctgatgaa
     4681 tttgaacgaa aaaacgggac ggcataccgt gaaattgaga tcgcattacc gcgagaactg
     4741 acacgtgaac aacggataga gcttgttgaa gatttcgtgc aaaaggaact tggtgatcgc
     4801 catgcctatc agtacgcgat ccacaatcca ccaggtgcaa ttgacgggaa ggaacagcca
     4861 cacgcgcata tcatgttttg cgaacggatt aatgatggta ttgaacgcga tccgcagcag
     4921 ttttttaaac gtgccaacag caaatcacca gagcgggggg gcgcaaaaaa ggcacgcttt
     4981 ggcgagacac aacaagagcg aaaatcagcc ctggttgcgc tacgctctca ctgggctgat
     5041 gtgcagaatg aacaccttgc gcgacacggc caccagtcgc gcgttgatca tcgttcgctg
     5101 aaagaacagg gtatcaagcg tacgccggag gtgcatcttg gtcccgttcg tgttgctgtt
     5161 atgaacgctg atcaggtttg tgcaattctg gcatggagaa atgcggaacg ggagaagctg
     5221 gaggccgaga aggaacggga cagcatcatt gaccttaaaa cgaccttgaa ggaagcgtta
     5281 gctgaacggg ataacatgcc accaaccctg gatgagcttg gggcagaatt gaaagcgttt
     5341 gttgaaagct cgtttgatga actcaatgag gtaatgcagc ttaattcatt gattgacggt
     5401 gcgctggaca caatgatgaa gcaattccag gtggaagagg agcggaagtt gcaacaggaa
     5461 cgactaattc aggctgcgcg agatgaagcc gaaaagcaaa gagagctgga gagggaaaga
     5521 gtgcgccggc gtgcgtattc ttcaccagca ccatcacgtt cacgcgatta tggtatgagc
     5581 cgttaagcac tggaggtttt ttattttatg caattggatg aacattcaag aaaaattgct
     5641 gcccttgttg cgtataatga aaagcaaata aaagaaataa acaggataat tactctgctt
     5701 aatgaaagag tgaatgactt aaataccttt acagatactc ttgaacgtga aggtaaagtt
     5761 gtatctggaa tttcttattc cttgagggat agtgtgtctc atgttgattt ttatcttaat
     5821 aaaggaatgg aagaagtatc ctgtaaatta cagaaatctg tctcagacac actaacatca
     5881 ttactccgtg aaaaattatc tttcacactt aatgatgtaa ggcaagagtg gactgatttt
     5941 gaagagcaaa caagtaaaga gataaaggaa tcattaaagc agacgaccag agatgctgaa
     6001 gttgcgacag gtataattag aaaaacaatg gagaagtata cagtaacaag gatagtcgaa
     6061 aaggtacttg tgttgcttgt attattttta ttgttaatga ttgctcctag tttttatgct
     6121 gaatatctca aaatgaatat cggttctcta atggaagaac agaaaaaaca tgaatctatg
     6181 ttaaaagagt ggaaggaaaa agcaccattg gcagagttct ctaagtgtgg agagtatagg
     6241 gacgtatgcg tgttagttga tgataaaagc ggatattatt ccaatgaaca aaccccaaat
     6301 aaaaaatatg ccatactgaa atagtgttaa gtaactcatg ccgtcttatg actaaggctg
     6361 catgagtata aaaacggcgt ttcacgtcaa gataaaataa atacctgtac ctaatataaa
     6421 aatggagcta catatactta tgtaccttga gaatgacgga gttaatttat cccttatcgt
     6481 caagctaata gctatgtaaa taatatctgt caggaatgat ataaatataa acaaaacacc
     6541 caatataaag aaatttaatg cgatatttgt attatcatct ataaatgctg gtattaatgc
     6601 cgcaaagaat aaatacgcct ttgggtttaa taatggtata agtaaaggtt tttgtacttt
     6661 gctttttttg ttttttttat taattaaacc cattacccct atgtatatta aatagcttcc
     6721 acctaagatc tttataatgt aaaagtgttg ttctatgctg gatgttgaag ataacgtgaa
     6781 aagagatatt accatgacga ttgcttcgcc agctcttatg tatatacttg tttttattga
     6841 tgatttaagt ccttgtgaaa aggcgttgtt tataaccaat gcaacaccag gtcctattga
     6901 tatagtttga gataaagtta cgatagcaaa tgaaaataat gttgaatatt ccatatttac
     6961 ttaccatgat atatttcgcg ggagcttttc atatacatat tccataggaa ttgcggcttt
     7021 cggtaataaa tttataaatg tggatatgtg ctttagtgaa ttgctggcgg cgattgagta
     7081 aaaatttccg ctattccaat atattgtcga tatttcgttg tccagactgt atatgtttgg
     7141 aaatttgttt gcatacgggt tcgttccgtt tagctggctt tttggatgag aaagtttgct
     7201 gttttgaccc gttcgcgctt ctgaaggggg gaaaccgatc atgaatcgct tttgtgcata
     7261 gttatgcagg cccataaaaa cgattctgac gcgtttttat ggtttaccct agtgttttct
     7321 tgtctttttg agtttttttg cgtcagaaag cgattgagag cgttttagag ggtacgtaca
     7381 acaggagtta tggtaaatgg atcgggtttg cgggaagact cgacaggttt tgtcgttggg
     7441 ggtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc gtgcctctcc gccaagattg
     7501 tcacgaagat tcaaggggcg tgtgggtcgt aacacacgga gggaatccta acacatgagc
     7561 gccgcgcttc aatacttcga agaaaattta ccccaccgtc cctatcacac ggatgatctc
     7621 gctttcggcc ttcgcatctc cggcaaaggg cgtgcgcttc ttgcgcggtt catccagcag
     7681 aaccagcctc atgcgcagtt ctggctggtt tttgacgttg accgcgaggg ggcagcgatt
     7741 gactggagcg accggaacgc ccccgcgccg aacatcaccg ttaaaaatcc tgtgaacgga
     7801 cacgctcacc tgctctatgc actcaatatc gccgtgagaa ccgcgcctga tgcgtcggtt
     7861 aaggcgctga aatatgccgc agcggttgaa cgttcgctgt gtgagaaact ttgtgctgat
     7921 gtgaactaca gcggactgat ttgcaaaaat ccgttccacc tggaatggca ggtgatggag
     7981 tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt gagcgcctca
     8041 gcgcgtcgta gcatcgataa acattacggg atggggcgaa actgccacct gttcgaaatg
     8101 acgcgcaaat gggcttacag ggcgattcgt cagggctggc cagcattctc acagtggctt
     8161 gatgccgtga ttcagcgtgt cgaaatgtac aacgcatcgc ttcccgttcc gctttctccg
     8221 cctgaatgtc gggctattgg caagagcatt gcgaaataca cgcacaggaa cttcacgccg
     8281 gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc agcacgcggt
     8341 cgcaagggcg ggaaggccaa ttcgtcagaa aatcagtcgg acaaagggaa aaaaagcgcc
     8401 gctgttcggt ggactgctaa cgacgacaaa cgtcgtaggg cgcttgatat gtatatcttg
     8461 ggagcgtcaa cggaagatat tgcagtggcg gttggtgtta gcagccggac tattcgacgc
     8521 tggatggata attctggaga atggttaacc aagaaacaaa taattaaatt ttaattaaca
     8581 gaaaaatgac aattaccccc tgtttttgtt aatgggtggt taaattcgcg gacatgaaca
     8641 agcctatatc agataacagc gcctttcggc gtctttttga gcagcgggct ttccggcggt
     8701 tttctcctgt cagcccagca atggtgcggc ttaccgcatg attgaggtgg ttgtgctcgc
     8761 cgcagtctca tgaccgagcg tagcgagcga atgagcgagg atagcaccag ggaaacggaa
     8821 cgtgaacgga ggctcttacg cactgtcttt ggttagtagc tccttcccgc gtctttccca
     8881 ttcctgccat tttgggtggt tgcgcggtag ctctgcgatt cctgcgagtg cgcctaccag
     8941 ttcggcagca ctaaactttg gttttccggt tttgctgtcc accagtcgtg cacaatcatg
     9001 aggcctgcgg ccttgtagag ttcatgatct cgtgcctttc gttccgccct tgcggctgcc
     9061 ttccgttctg ccgctatgat tgccgttact ctggcttttg ctgcggctgc tttctctgcg
     9121 gttttttctg cccgtatcag tagcggacaa cgttttttct atccgtcgtt gttcggtttt
     9181 ttttctctgc cagtaacaca aggtaatctc tgctgctctg acggtgattt cagtccgttg
     9241 atgtaggcaa tttttgcact taaccagtct tcacgatttg tcatccttac ctctgcactt
     9301 ttgccgttaa tgtgagttgt tattgtgcat aatatcacct tgtctgcctg cgtgttagac
     9361 cgtaaaatca tccctgatta attcagggat gcgcacttag acgtttctct cctttgtcga
     9421 gaaacagtgc gctcctgcgg agggcttaac ggcagatggc aagctaccat tgcacagtta
     9481 aaactggagc aaaaggcgta gccttaaaac atgctgatta catttccaga tccggcgaat
     9541 ataaaaacta taaatcgcgg gaggatctgg aatttcttca tccggtaaca tgccatcatg
     9601 ggcgaagaaa aatcccgcag aactgtggaa agctgctgat gaatttgaac gaaaaaacgg
     9661 gacggcatac cgtgaaattg agatcgcatt acgcgagaac tgacacgtga acaacggata
     9721 gagcttgttg aagatttcgt gcaaaaagga acttggtgat cgccatgcct atcagtacgc
     9781 gatccacaat caccaggtgc aattgacggg aaggaacagc cacacgcgca tatcatgttt
     9841 tgcgaacgga ttaatgatgg tattgaacgc gatccgcagc agtttttaaa cgtgccaaca
     9901 gcaaatcacc agagcggggg gggccgcaaa aaaggcacgc tttggcgaga cacaacaaga
     9961 gcgaaaatca gccctggttg cgctacgctc tcactgggct gatgtgcaga atgaacacct
    10021 tgcgcgacac ggccaccagt cgcgcgtgat catcgttcgc tgaaagacag ggtatcaagc
    10081 gtacgccgga ggtgcatctt gtcccgtccc tgttcgtgt
//
