LOCUS       CP088854                6647 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain A16EC0554 plasmid pA16EC0554-3, complete
            sequence.
ACCESSION   CP088854
VERSION     CP088854.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391544
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 301x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:51:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,342
            CDSs (total)                      :: 5,224
            Genes (coding)                    :: 5,000
            CDSs (with protein)               :: 5,000
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 86 of 224
            Pseudo Genes (incomplete)         :: 173 of 224
            Pseudo Genes (internal stop)      :: 40 of 224
            Pseudo Genes (multiple problems)  :: 63 of 224
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="A16EC0554"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pA16EC0554-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.57 N 126.98 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(9..478)
                     /locus_tag="LQT70_26640"
                     /pseudo=""
     CDS             complement(9..478)
                     /locus_tag="LQT70_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing N-terminus and C-terminus; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     gene            complement(504..1201)
                     /locus_tag="LQT70_26645"
     CDS             complement(join(504..952,952..1201))
                     /locus_tag="LQT70_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="UGK29770.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(1257..1340)
                     /locus_tag="LQT70_26650"
                     /pseudo=""
     CDS             complement(1257..1340)
                     /locus_tag="LQT70_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     gene            complement(1392..2000)
                     /locus_tag="LQT70_26655"
     CDS             complement(1392..2000)
                     /locus_tag="LQT70_26655"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29771.1"
                     /translation="MLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGHGEMVV
                     SVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAACFNRWDLPLSDM
                     YTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWL
                     NIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
                     /name="hypothetical protein"
     gene            complement(1888..2151)
                     /locus_tag="LQT70_26660"
     CDS             complement(1888..2151)
                     /locus_tag="LQT70_26660"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29772.1"
                     /translation="MKNKLLTRCAIQFTRAPLDNDIGVSEATRIDPNAWVETLEGGWPL
                     PGRSGVVAVHGRYTCRRGADYDRPRVAASGENLIYQPENLPD"
                     /name="hypothetical protein"
     gene            complement(2243..2362)
                     /locus_tag="LQT70_26665"
     CDS             complement(2243..2362)
                     /locus_tag="LQT70_26665"
                     /inference="COORDINATES: protein motif:HMM:NF027672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29773.1"
                     /translation="MQPNATAWSEAGHISAWQQWRLAENLSVTLPSASTPSLN"
                     /name="hypothetical protein"
     gene            complement(2457..2501)
                     /locus_tag="LQT70_26670"
                     /pseudo=""
     CDS             complement(2457..2501)
                     /locus_tag="LQT70_26670"
                     /inference="COORDINATES: protein motif:HMM:NF027672.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2640..3460)
                     /gene="lacZ"
                     /locus_tag="LQT70_26675"
                     /pseudo=""
     CDS             complement(2640..3460)
                     /gene="lacZ"
                     /locus_tag="LQT70_26675"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="forms a homotetramer; hydrolyzes lactose
                     disaccharide to galactose and glucose; converts lactose to
                     allolactose which is the natural inducer of the lac operon;
                     frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /name="lacZ"
     gene            complement(3430..3684)
                     /locus_tag="LQT70_26680"
     CDS             complement(3430..3684)
                     /locus_tag="LQT70_26680"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29765.1"
                     /translation="MWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPELWSAE
                     IPNLYRAVVKLHTADGTLIEAEACDVVSSRCGLKMVCCC"
                     /name="hypothetical protein"
     gene            complement(3766..4294)
                     /locus_tag="LQT70_26685"
                     /pseudo=""
     CDS             complement(3766..4294)
                     /locus_tag="LQT70_26685"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4218..4460)
                     /locus_tag="LQT70_26690"
     CDS             complement(4218..4460)
                     /locus_tag="LQT70_26690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29766.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEACAGKLAGVRSS"
                     /name="hypothetical protein"
     gene            complement(4581..4820)
                     /locus_tag="LQT70_26695"
     CDS             complement(4581..4820)
                     /locus_tag="LQT70_26695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29767.1"
                     /translation="MLYPAVTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVK
                     EKPPFRPIRKPPLPARCRFIMQLARQVSRLESGQ"
                     /name="hypothetical protein"
     gene            complement(4802..5254)
                     /locus_tag="LQT70_26700"
     CDS             complement(4802..5254)
                     /locus_tag="LQT70_26700"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="UGK29768.1"
                     /translation="MEAACANVPAYFLMSLTRLPSTVLFSPMKTVRAGRVEHWSHWGHQ
                     QIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITERKATGVPCPVFQQTMQMLNEGIVP
                     TAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPP"
                     /name="substrate-binding domain-containing protein"
     gene            complement(5286..5534)
                     /locus_tag="LQT70_26705"
     CDS             complement(5286..5534)
                     /locus_tag="LQT70_26705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGK29769.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERAASKPVKRRCTIFSRKRQWADH"
                     /name="hypothetical protein"
     gene            complement(5734..6560)
                     /locus_tag="LQT70_26710"
                     /pseudo=""
     CDS             complement(5734..6560)
                     /locus_tag="LQT70_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /name="DNA-binding transcriptional regulator"
ORIGIN
        1 catccgtttt tggcgaaaaa gagtaaaacg gcgagatgag tgcacagcca gaacccagcc
       61 agaaaacaac tgattattga tggtgacatg atgccgacaa tcgaggccac agcgcccagc
      121 caacacagcc aaacacagcg cgcgaccaaa ttcgaatgac tgcgacggct gactttctcg
      181 ataaatgcct ctactgctgg cgcaccggcg tttaaaacaa aagccaagat aaataccacc
      241 aacaatcgat cctactaaaa tgttgtattg taacagtggt ccgaagataa aaataaagac
      301 ggcgcaaaac atcactaaca tgccggtaat aatccacagc aggtatttgc gcagcccgag
      361 ttttcagaaa cagacccaaa cagcggttgg aataatagcg agaacagaga atagcggcaa
      421 aaataatacc cgtatcactt ttgctgatat ggttgatgtc atgtagccaa atcgggaaaa
      481 cggaagtagg taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg
      541 accttgtcat gcagctccac cgattttgag aacgacagtg acttccgtcc cagccttgcc
      601 agatgttgtc tcagattcag gttatgccgc tcaattcgct gagtgtaacg cttgctgatt
      661 acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt catccatacc
      721 acgacctcaa aggccgacag caggcttaga agacgctcca gtgtggccag agtgcgttca
      781 ccgaatacgt gcgcaacaac cgtcttccgg agcctgtcat acgcgtaaaa cagccagcgc
      841 tggcgcgatt tagccccgac gtatccccac tgttcgtcca tttccgcgca gacgatgacg
      901 tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt aaatggcgga
      961 aaatcgtgtt gaggccaacg cccataatgc gggcggttgc ccggcatcca acgccattca
     1021 tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca
     1081 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc
     1141 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
     1201 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcacccggg
     1261 aagtaggctc ccatgataaa aaagtaaaag aaaaagaata aaccgaacat ccaaaagttt
     1321 gtgtttttta aatagtacat aatggatttc cttacgcgaa atacgggcag acatggcctg
     1381 cccggttatt attatttttg acaccagacc aactggtaat ggtagcgacc ggcgctcagc
     1441 tggaattccg ccgatactga cgggctccag gagtcgtcgc caccaatccc catatggaaa
     1501 ccgtcgatat tcagccatgt gccttcttcc gcgtgcagca gatggcgatg gctggtttcc
     1561 atcagttgtt gttggctgta gcggctgatg ttgaactgga agtcgccgcg ccactggtgt
     1621 gggccataat tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg gaagacgtac
     1681 ggggtataca tgtctgacaa tggcagatcc cagcggttaa aacaggctgc agtaaggcgg
     1741 tcgggatagt tttcttgcgg ccccaggccg agccagttta cccgctctgc gacctgcgcc
     1801 agctggcagg tcaggccaat ccgcgccgga tgcggtgtat cgctcgctac ttcaacacct
     1861 acactgacga ccatctcgcc gtgtccatca atccggtagg ttttccggct gataaataag
     1921 gttttcccct gatgctgcca cgcgtgggcg gtcgtaatca gcaccgcgtc ggcaagtgta
     1981 tctgccgtgc actgcaacaa cgccgcttcg gcctggtaat ggccagccgc cttccagcgt
     2041 ttcgacccag gcgttagggt caatgcgggt cgcttcactt acgccaatgt cgttatccag
     2101 cggcgcacgg gtgaactgaa tcgcgcagcg ggtcagcagt ttgtttttca tcgccaatcc
     2161 acactgtgaa agaaagcctg actggcggtt aaattgccaa cgcttattac ccactcgatg
     2221 caaaaatccg tttcgctggt ggtcagttga gggatggcgt ggacgcggag gggagtgtca
     2281 cgctgaggtt ttccgccaga cgccattgct gccaggcgct gatgtgtccg gcttctgacc
     2341 atgcggtcgc gtttggttgc actacgcgta ccgttagcca gagttgtccg gcgctctccg
     2401 gctgcggcag tttcaggcag ttcaatcaac tgcttacctt gcgggccaac atccagaggc
     2461 acttcaccgc ttgccagcgg cttgccatcc agcgccacca tccagtgcag gaactcgtta
     2521 tcgctatgac ggaacaggta ttcgctggtc acttcgatgg ttcgcccgga taaacggaac
     2581 tggaaatact gctgttggtg ttttgcttcc gtcagcgccg gatgcggcgt gcggtcggca
     2641 aagaccagac cgttcataca gaactggcga tcgttcggcg tatcgccaaa atcaccgccg
     2701 taagccgacc acgggttgcc gttttcatca tatttaatca gcgactgatc cacccagtcc
     2761 cagacgaagc cgccctgtaa aacgggggta ctgacgaaac gcctgccagt atttagcgaa
     2821 gccgccagac tgttacccat cgcgtgggca tattcgcaaa ggatcagcgg gcgcatttct
     2881 ccaggcagcg aaagccattt tttgatggac catttcggca ccgccggaag ggctggtctt
     2941 catccacgcg cgcgtacatc gggcaaataa tatcggttgc ggaggtgtcg gctccgccgc
     3001 cttcatactg taccgggcgg gaaggatcga cagatttgat ccagcgatag agtgcgtcgt
     3061 gattagcgcc gtggcctgac tcattcccca gcgaccagat gatcacactc ggtgattacg
     3121 atcgcgctgc accatccgcg ttacgcgttc gctcatcgcg gtagccagcg cggatcatcg
     3181 gtcagacgat tcattggcac catgccgtgg gtttcaatat tggcttcatc caccacatac
     3241 aggccgtaaa cggtcgcaca gcgtgtacca cagcggatgg ttcggataat gcgaacagcg
     3301 cacggcatta aagttgttct gcttccatca gcaggatatc ctgcaccatc gtctgctcat
     3361 ccatgacctg accatgcaga ggatgatgct cgtgacggtt aacgccgcga atcagcaacg
     3421 gcttgccgtt cagcagcagc agaccatttt caatccgcac ctcgaggaaa cgacatcgca
     3481 ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt ttaaccaccg cacgatagag
     3541 attcgggatt tcggcgctcc acagttccgg attttcaacg ttcaggcgta gcgtgacgcg
     3601 atcggcataa ccgccacgct catcgataat ttcaccgccg aaaggcgcgg tgccgctggc
     3661 gacctgcgtt tcaccctgcc acaaaaaacc gtcacccgca gttcatcgcg cagctcgccg
     3721 tacatctgaa cttctgcctc cagtaccgcg cggctgaaat catcattaaa gcgtggtaac
     3781 ttggaaatcg ctgatttgcg tggtcggttt atgcagcaac gagacgtcac ggaaaatgcc
     3841 gctcatccgc cacatatcct gatcttccag ataactgccg tcactccagc gcagcaccat
     3901 caccgcgagg cggtttctcc ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa
     3961 cggctgtcct ggccgtaacc gacccagcgc cgttgcacca cagatgaaac gccgagttaa
     4021 cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca acattaaatg
     4081 tgagcgagta acaacctgtc ggattctccg cgggaacaaa cggcggattg accgtaatgg
     4141 gataggttac gttggtgtag atgggcgcgt cgtaaccgtg catctgccag tttgagggga
     4201 cgacgacagt atcggcgtca ggaagatcgc actccagcca gctttccggc acacgcttct
     4261 ggtgccggaa accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     4321 cgatcggtgc gggcctcttc gctatttcgc cagctggcga aagggggatg tgccgcaagg
     4381 cgattaagtt gtgtaacgcc agggttttcc cagtcacgac gttgtaatac gacggccaga
     4441 gaatccgtaa tcatagtcat agctgtatcc tgtgtgaaat tgttatccgc tcacaatttc
     4501 acacacatac gagccggaca catagagtgt aaagactggg tgcctaatga gtgagctgac
     4561 tcacattaat tgtgttgcgc tcactgcccg ctttccagtc gggaaacttg tcgtgccagc
     4621 tgcattatga atcggcaacg cgcggggaga ggcggtttgc gtattgggcg gaagggtggt
     4681 ttttctttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     4741 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     4801 gttacggcgg gatataacac gagctgtctt cggtatcgtc gtatcccact accgagatat
     4861 ccgccccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
     4921 cgttggcaac cagcatcgca gtgggaacga tgccctcatt tagcatttgc atggtttgtt
     4981 gaaaaaccgg acatggcact ccagtcgcct tccgttctgt tatcggctga atttgattgc
     5041 gtgtgagata tttatgccag cccgccagac gcagacgcgc cgagacagaa cttaatgggc
     5101 ctgctaacag cgcgatttgc tggtgacccc aatgcgacca gtgctccaca cgcccagctc
     5161 gtaccgtctt catgggagaa aataatactg ttgatgggag tctggtcaga gacatcaaga
     5221 aataagccgg aacattagcg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
     5281 gatagttaat gatcagccca ctgacgcttg cgcgagaaga ttgtgcaccg ccgctttaca
     5341 ggcttcgacg ccgctcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     5401 cgagatttta tcgccgcaac aatttgcgac ggcgcgtgca gggccagact ggaagtggca
     5461 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     5521 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt ttcgctgaaa cgtggcaggc
     5581 ctggttcaca cacgcgggaa acggtctgat aagagacacc ggcatactcg cgacatcgta
     5641 tagcgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     5701 cataccacga aaggttttgc gccattcgat gtgtcagcgt aaatgcacgc cgcttcgcct
     5761 tcccgctacc agaatagcct gcgattcaac cccttcttcg atctgttttg ccacccgttg
     5821 cagcgccgga agatgctttt ccgctgcctg ttcgatggtc attgcgctcg ccatatacac
     5881 cagattcaga cagccaatca cccgttgttc actgcgcagc ggtacggcga tagaggcgat
     5941 cttctcctcc tgatcccagc cgcggtagtt ctgtccgtac ccctctttgc gcgcgcgcgc
     6001 gaatggcttc cagctttaac ggttctcgtg ccagttgata gtcatcaccg ggcgggaggc
     6061 taacatctcg attaattcct tgcgttcttg ttccgggcaa aaggccagcc aggtcaggcc
     6121 cgaggcggtt ttcagaagcg gtaaccgtcg cccgaccatt gcccggtgaa aagataagcg
     6181 gctgaaacga tgggtggttt cgcgtaccac cattgctcaa catccagcgt ggacacatct
     6241 gtcggccata cacttcgcgc aacagatcgc ccagcagcgg ggccgccagt gcagaaatcc
     6301 actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcaga cgaaaactgt
     6361 catcggaggt actgcgccgg acatatccct cttccagtaa cgtctccagc agtcgccgca
     6421 cagtggtgcg atgcaggcgg ctgagttccg cagcagcccg acgctgcacc gccatcaagt
     6481 ttatttaaca tatttaataa cattagaccg cgggttaagc cgcgcacggt tttgtattcc
     6541 gtctgctcat tgttctgcat attaattgac attatatagt taaaacaacg ttgtgcacct
     6601 ggtgcacatt cgggcatgtt tgattgtagc cgaaaacacc cttccta
//
