LOCUS       CP088817                3137 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0262 plasmid pB16EC0262-5, complete
            sequence.
ACCESSION   CP088817
VERSION     CP088817.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391557
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:25:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,091
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,766
            CDSs (with protein)               :: 4,766
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 76 of 201
            Pseudo Genes (incomplete)         :: 147 of 201
            Pseudo Genes (internal stop)      :: 42 of 201
            Pseudo Genes (multiple problems)  :: 55 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0262"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0262-5"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(2571..3137,1..361)
                     /locus_tag="LQQ73_25445"
                     /pseudo=""
     CDS             join(2571..3137,1..361)
                     /locus_tag="LQQ73_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            364..897
                     /locus_tag="LQQ73_25450"
     CDS             364..897
                     /locus_tag="LQQ73_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ76009.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGWVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPATGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2000..2395)
                     /locus_tag="LQQ73_25455"
     CDS             complement(2000..2395)
                     /locus_tag="LQQ73_25455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ76008.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     Typing          39..358
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="96.0"
                     /evalue="1.73e-61"
     Typing          2572..3137
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.354"
                     /coverage="96.0"
                     /evalue="3.99e-124"
ORIGIN
        1 ggctttgaaa ggggcttcat tgactgccgg acgctgaaaa gaacagggca ttgaacgacg
       61 tcccgaagtg catctgggtt ttgaagctgc cggacggctt gatgatgccc agcgtcatga
      121 catcatgcaa aaacggtctg aaccggacta ttcccgccat cttgaacagg cgcgaacggt
      181 cgggcatgac atcagggaat ggctggacac tccggttaag caacatgaac tggtcagaag
      241 gctgcaacat gatgttgcgc gggagcatgg ctttgacatg agagacagag tgagcagaca
      301 gaaccgaact gaacctgaac gggttaaatc accgtcacgt ggattttcta tgggattctg
      361 aacatggcca gcacagaaga aaaattactc acgctcctgt tatcggtgga gcatctgcaa
      421 cataccgcaa tggagcaaca gaaagctgtt gctgattctc tggctgtggc aagggaaaca
      481 cttcgcggat atcgggatat ttctgacggt gtggcaaaac aggtcaggga tgaagtcaga
      541 aacgaggtgc agaagctgga tgttgcaggg ctgataggtg agcgcatcag ccaggatttt
      601 cagcggctgg aagagtcagt cgcaaacatg aaggcaaacg cggatgccct gaacagggat
      661 attgcgacaa ttcacagcaa tatcgtgcag gaatacaaca cccttaaagg gtatagctgg
      721 aaatggctgg gttgggtgtc agtggctttt ctggttatgt tctgtgtgct tggatgggga
      781 atgaaaacaa tgctcgacag gaactacaac gccacagcgg cggtgtatca gaaagttgtg
      841 acactggaag ataaactggc ggagaaacca gcaacaggga agagccggaa aaactgacgc
      901 gcttcgcttg tccaacgcct cacgggacgc gaaaaaattc cgcgtcccgt tcaccgtttt
      961 ctgatgagaa agtttgccgt tttgacccgt tcggtgctca gatttggcga aattgattcc
     1021 gaatcacttt tgtgaatagt tatgcaagcc cctggaaacg tttctgacgc gttttttgag
     1081 gtaggggtgg gggaaagcat ggttttttga tgttttgagc gtaaaagcgt gaaaaatcgc
     1141 gttattgtgc ttgcgatctt tcgtcaggga gcgtagaacg aaaaaagccc gccgttaggc
     1201 gagcttcttc gggttcattg aggttttcga cctcctcaat gttcctgtgg agtctgttgc
     1261 ggtgacaacg gaaaccagtt cattcagttt gcatgaacct tctgtattct tcaagcatta
     1321 ccgaccgcac agcctcccca gaatcccgaa aaaatcctgt ttttgcagat gattgcgtgt
     1381 ttgcaaagac cgtttgcggt tgggggagct gtgacgacgg caaccgcacg cgtgagagtt
     1441 ccgctaccgg cgcagagagt ttcatcgaca gcggacacct gatgagttgc cacaaaaacc
     1501 gtatgtagcg cggctagatt ggttgagtta gaccttggcg gcagttcccg gtatgtggtg
     1561 gcgtgacggg tgagatggtg cggcgaagac gcatacgtca cgcaggcgcg ggttcttcca
     1621 ccgacgcgcc ccgtgatcga tggccttggc tccgtccggc atatcgagag cggcaaacta
     1681 cccgtccctt cactgggagg ggtgagttgt tgccgttccc tccgaccttc caccatccag
     1741 catatcgcac gatcccatga gcaccgcaac aactgataac tccagagcaa aaaaaaaaaa
     1801 ggatctgcat catgtgagag tgatcccacc gctcgccgca gccccagcga ccgaacgtag
     1861 tgagcgagtg ggcgaggaag cggaagtgaa ccggaaacca cattgagcac gtacgcacct
     1921 atgccagatc cgcgcgtagc gcgtcgcgaa atcgaaaaca ttgaacaccg gcaggcaaac
     1981 gcaccaccac gcagcggaat taatgatgat ctgggcttgt ttcaagatcg agcctgattg
     2041 ccttaagttt ctgtaatata ttgtcttttg ggtatctcag ttcgcctgtt tttgtgtcca
     2101 ctagtccggc ctcgatcagt aaccccgccg caagaaacag gcggtgattg cgttcccgtc
     2161 gttgtgtttt cccctccatc cgtttttggt tggcctgttt gcgtgcgttc tcctgtgcct
     2221 gtagctcgtt tttttctgcg gttgccagga cgtggagtag tttgcgctcc tgttctgtta
     2281 gtccggcttc gttatcgagc aggtcaatga tctgcctctg tcgtgcggtc tgttctccga
     2341 gcttcctgat aaagccgatt cgtttcttca accagtcgat gttgctgatg cgcatgtttg
     2401 ggatgctccc tggtgttttt tgcggtgtgt tggggttgtg cagtatatcg cttgcggtcg
     2461 gtgggtgcag ggggtatctt ccgtgctaat tcgtaaaatt agaaggaagc gcacttatat
     2521 gtttctctcc ttcgtcgaga aacagtgcgc tcctgcggag ggctgaaacc gtggcaacct
     2581 atcacctcag cgttaaattt ggtggcaagg ggcaggctgc aaaccacgcc gattacatcg
     2641 agcgcaaaga gaaataccgc gaccgtcagg atttggaata ttccgcacac ggaaacatgc
     2701 cggaatgggc gcgagataat ccctcacatt tctggcaggc ggctgaccag tttgaacgtg
     2761 cgaacggttc gacctatcgg gaacttgaaa ttgcattacc ccgtgaactg acagcggaac
     2821 agcgccttga actggtgcag gattttgtac gtcaggaagc tggcgaacgg cacgcctgga
     2881 gttttgccat tcacaatccg aaagcgagca ttgacggcgg cgaacagcct catgcgcata
     2941 tcatgatgag ccagcgtgtg aatgatggca ttgaacgtac accggaacag tatttccggc
     3001 gctataacgc caggtatccc gagcggggag gtgcgaaaaa ggattccgga agcctgactc
     3061 tcactcagca aaaagagcag ttgcgggaat tgcgaaaacg ctgggaggtg aagcacaacg
     3121 aacatatgag aaagcat
//
