LOCUS       CP088816                4014 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0262 plasmid pB16EC0262-4, complete
            sequence.
ACCESSION   CP088816
VERSION     CP088816.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391557
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:25:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,091
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,766
            CDSs (with protein)               :: 4,766
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 76 of 201
            Pseudo Genes (incomplete)         :: 147 of 201
            Pseudo Genes (internal stop)      :: 42 of 201
            Pseudo Genes (multiple problems)  :: 55 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0262"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0262-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(3049..4014,1..234))
                     /locus_tag="LQQ73_25415"
     CDS             complement(join(3049..4014,1..234))
                     /locus_tag="LQQ73_25415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGJ76002.1"
                     /translation="MALYRLEMQNVSRANGVSSVAKAAYRHRSVMVDHRTGEIHGEKSA
                     NRDDLVYAEILAPDNTPDFLIKSSNDLWGFVEQTERRKDARTAKEFKITLPHELSNEQN
                     IALMKDFLLNHFVDKGIICDFVLHNDKDNKNPHAHVMITTREITPTGFGKKVRSWDEEK
                     TLHEWRKDWSKVQNRHLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNMSRKKWRSKEGQEQRKRDQAIRDRAKQDARAVRFLRGVAVVDVASFT
                     VHPLEKKEHSNNNDFSVKKSWWRSTVNFIKNIKAKFSTKKDIKNDVHASDDLMTDPVTG
                     LIITKQKWQEMSDRASQKAPEATISYQVKQSHAESEKTPQNALQSDLDSVAVRRRRPKI
                     "
                     /name="MobA/MobL family protein"
     gene            530..925
                     /locus_tag="LQQ73_25420"
     CDS             530..925
                     /locus_tag="LQQ73_25420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ76003.1"
                     /translation="MDKKEIEKRLLIFTSEIDIEDIKNDFINNEINEDAFTGYLKLYYA
                     IDNAIHNNKRSLFEVLIYLINKDRENKDHNEPIITDYDLVYLTLNNYINIYELKRLFLL
                     QKNINDFDFKSMMSEIEKIKREVENGK"
                     /name="hypothetical protein"
     gene            915..1193
                     /locus_tag="LQQ73_25425"
     CDS             915..1193
                     /locus_tag="LQQ73_25425"
                     /inference="COORDINATES: protein motif:HMM:NF018152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="UGJ76004.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASQKERKARDHKKFIAGGEVT
                     RVFGLDYDEAVLLGFLAIYDKLKPEQKAQIKIDGENIIKSRLENADK"
                     /name="conjugal transfer protein TraD"
     gene            1326..1592
                     /locus_tag="LQQ73_25430"
     CDS             1326..1592
                     /locus_tag="LQQ73_25430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ76005.1"
                     /translation="MSQKKEQKTAEKPTAGPLRKHRTRTEKIDDLKKQISELRKAEREE
                     RQRARDEKLIILAKAVYEKVGKPKDIDALIEFVKSEDFKIEYK"
                     /name="hypothetical protein"
     gene            complement(1606..1878)
                     /locus_tag="LQQ73_25435"
     CDS             complement(1606..1878)
                     /locus_tag="LQQ73_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UGJ76006.1"
                     /translation="MAAIRATKRNKTAKDLAKKLGVHERTVRRYIALPRSDYESEAESR
                     RKTAYNLHFKDGLKWRDVAEKMNATEHAVKALAKRYKQIDQEKRG"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1884..2738)
                     /locus_tag="LQQ73_25440"
     CDS             complement(1884..2738)
                     /locus_tag="LQQ73_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGJ76007.1"
                     /translation="MLKQEEYNRDKYINQIQSWTLCADDFENGTYRKPKEKALNKKYIG
                     YNNNYFINGFVFDIDQPQGAIAWDVCGLPKPNIIIQNTDNGHAHLLYALKAPVCKTDNA
                     RIKPLKLASLIQKGFTDRLKADRAYADVLMKNPINEAEWRTTWGNIEAYDLSYLSEFIP
                     DDIQEQKRLPADGLGRNVNLFEDLRHYAYKSVLKFKEKSNYENWEKELILKADGLNTFC
                     NSSNLLSYNEIKATAKSVAKWTWRNFNKKQFSCIQSYRGKKNTGKTKKSSRMLDMIMKA
                     DAE"
                     /name="replication initiation protein"
     Typing          complement(3086..4014)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.806"
                     /coverage="92.0"
                     /evalue="1.15e-144"
ORIGIN
        1 ttgttcgaca aaaccccata aatcgttact cgatttaatg agaaaatcgg gcgtgttatc
       61 tggtgctaat atttcagcat aaactagatc gtcacgatta gctgatttct cgccgtgtat
      121 ttctcccgtt ctatggtcaa ccataaccga acgatgacga taagccgcct ttgcaactga
      181 gctgacacca tttgcacggc ttacgttctg catttctaac ctatataaag ccatatatta
      241 acacctcata aattgaaccc cttttgtttt ttgggttggg tttaaagttt gacgcaagga
      301 aaacttggtt tttagctccg caggacccgg cattgtttac aatgcgtaac cgggcattcc
      361 ttatctttta ttttttcgct gacgctcaaa ctaaaagagg aatgaaaacc aaactagctt
      421 ttctttcgct gatatatata aattaactct gattaaatta aagtcaaatt tgacaatatt
      481 taaaagttac gatattgaat aaaatataat ctatattttg ggtgataaaa tggataaaaa
      541 agaaatagaa aaaagactat tgatttttac aagcgagatc gacattgaag atattaaaaa
      601 cgatttcatc aataacgaaa ttaatgaaga tgcttttact ggctatttaa agctgtatta
      661 cgctatcgat aatgcaatac acaacaataa aagatcattg tttgaagtgt tgatatattt
      721 gataaataaa gatcgtgaaa ataaagacca taatgaacca attataactg attatgatct
      781 cgtgtattta acactaaata attacataaa tatctatgag ttgaaaaggt tgtttttatt
      841 acaaaaaaat atcaatgatt ttgattttaa aagtatgatg tcagaaatag aaaaaattaa
      901 acgagaggtt gaaaatggga agtaaaaaaa cgttagaaca aagaatggca cttgcacaag
      961 atagattatc taaacttaaa aaagaagcat cgcagaaaga aagaaaagct agagatcata
     1021 aaaaatttat tgccggagga gaagttacta gagtcttcgg attagattat gatgaagccg
     1081 ttttgctggg ttttctagct atctatgaca agctgaaacc tgagcaaaaa gcacagataa
     1141 aaattgatgg tgaaaacatc ataaaatcac gtctagaaaa cgctgacaag tgacagattt
     1201 ctaatctgac acttggaagg gttgaacaag caagaacccg ctacaagcgg ggcttgtgcg
     1261 ttagtcttca cgatagcctg acgttgtgat atgttgtaaa aaacatatca gatcaggagt
     1321 gaaaaatgtc tcaaaaaaaa gagcaaaaaa cagcagaaaa accaactgca gggccactga
     1381 gaaaacacag aactagaact gaaaaaatag acgatttaaa aaagcagatt agcgaactaa
     1441 gaaaagcaga aagagaagaa cgacaacgag caagagacga aaaattaatt attcttgcaa
     1501 aagctgttta tgaaaaagta ggaaagccga aggatatcga tgcattgatt gagtttgtaa
     1561 aatcagaaga tttcaagatt gaatataaat aataataggg gcattttagc cccttttttc
     1621 ttggtcgatt tgtttgtatc tttttgctaa tgctttaact gcatgttctg ttgcattcat
     1681 tttttcagca acatcacgcc atttcaatcc atccttaaaa tgtagattgt atgctgtttt
     1741 tctacggctt tcggcttctg attcataatc agaacgtggt aatgcaatat aacgccttac
     1801 cgttctttca tgaacaccta gtttctttgc taaatcttta gctgttttat tcctttttgt
     1861 tgctcttatt gctgccatag taattactcc gcatctgctt tcatgatcat gtctaacata
     1921 cgactgcttt tttttgtctt tcctgtgttc ttttttcctc tgtatgattg tatgcatgaa
     1981 aactgttttt tattgaagtt tcgccatgtc catttggcta ctgattttgc tgttgctttt
     2041 atctcattat agcttaatag attactacta ttacagaatg tatttaatcc atcagctttt
     2101 aatatcagtt ctttttccca attttcatag ttactttttt ctttaaattt taaaacggat
     2161 ttatatgcat aatgtctaag atcttcaaac agattaacat tgcgtcctag accatctgca
     2221 ggcaatcttt tttgttcttg aatgtcatca ggtataaact cgcttagata gctgagatca
     2281 tacgcttcaa tatttcccca tgttgtacgc cattcagctt cattaattgg gtttttcatt
     2341 agcacatcag catatgcacg atctgctttt aatctatctg taaatccttt ttgaatcagg
     2401 cttgcaagtt taagcggctt tattcttgca ttatcagttt tacatacagg agcttttaaa
     2461 gcataaagca aatgagcatg accattatca gtattttgaa taattatatt gggcttaggt
     2521 aatccgcaaa catcccacgc aattgcacct tgaggttggt ctatatcaaa cacaaaacca
     2581 ttaataaaat agttgttgtt gtaacctata tattttttgt ttaacgcttt ttcttttggc
     2641 tttctgtatg ttccattctc gaaatcatct gcacacaaag tccaactttg aatttgattt
     2701 atatacttat ctctgttata ttcttcttgt tttaacatat tttgttttcc cttttttata
     2761 tgcccccggt tttttttcct gtcgccaaac atcgaaaaaa ccgggggttt ttgtttctgt
     2821 tactgttcta acacttttta caagtgtgtc aagtcgggca gttaaccata tcagatagtg
     2881 atcggttttt agggcttttt ggcgaagcac aggccggagg cttccaataa tcaccagtaa
     2941 atctaagtcc taactctcaa tgcaggatct ggctgtgctg gttagctgag aaccgcaccg
     3001 gctctatggt atgtgcacat cacttcctga aactcgacca ccctgcagct agatcttcgg
     3061 tctgcgtctt ctaactgcaa cactgtctaa atcgctctgt aacgcattct gcggcgtttt
     3121 ctcactttca gcgtgtgatt gcttcacttg atacgagatc gtggcctctg gtgctttctg
     3181 tgaagctctg tcactcatct cttgccattt ttgcttcgta ataatcaaac cagtgacggg
     3241 atcagtcatt aaatcgtctg atgcatggac atcgttttta atatcttttt tagttgagaa
     3301 cttagcttta atatttttta taaaattaac cgtagaacgc caccacgatt ttttaactga
     3361 aaaatcatta ttatttgaat gctctttttt ctctaacgga tgaactgtga atgaagcaac
     3421 atcaacaact gcaacacctc tcaaaaacct gacagccctt gcatcctgct tcgctctatc
     3481 tctgatcgct tggtcacgtt tgcgttgctc ttgcccctct ttagaacgcc actttttacg
     3541 gctcatgttc tgcattggtg gtcgatctag ttcaatagct tttgctaaat ataaaataga
     3601 cttgtcaaaa tcctttgcat cttctgcttt tttagctaaa tcgatcatta tgtttttttg
     3661 ttcttctagc gttctatgac ttacacgaga ttttaagcca gcatttttta aatgtctgtt
     3721 ttgaactttt gaccaatctt tacgccattc atgcaaggtt ttttcttcat cccatgatct
     3781 tactttttta ccaaatcccg ttggcgttat ttctcttgtg gtgatcatca catgagcatg
     3841 aggattttta ttatctttgt cattatgtaa aacaaaatca catataatgc ctttatcaac
     3901 gaaatgattt aataaaaaat ctttcatcaa tgcgatgttt tgttcattcg ataactcatg
     3961 cggcaaagtg attttgaatt cttttgcagt tctagcatcc tttctcctct ctgt
//
