LOCUS       CP088815                4082 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0262 plasmid pB16EC0262-3, complete
            sequence.
ACCESSION   CP088815
VERSION     CP088815.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391557
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:25:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,091
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,766
            CDSs (with protein)               :: 4,766
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 76 of 201
            Pseudo Genes (incomplete)         :: 147 of 201
            Pseudo Genes (internal stop)      :: 42 of 201
            Pseudo Genes (multiple problems)  :: 55 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0262"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0262-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            47..2206
                     /locus_tag="LQQ73_25400"
     CDS             47..2206
                     /locus_tag="LQQ73_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGJ76001.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQRRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(2462..3163)
                     /locus_tag="LQQ73_25405"
     CDS             complement(2462..3163)
                     /locus_tag="LQQ73_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGJ75999.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3696..3950)
                     /locus_tag="LQQ73_25410"
     CDS             complement(3696..3950)
                     /locus_tag="LQQ73_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UGJ76000.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          47..405
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3952..4082)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.09e-62"
     Typing          complement(2463..3163)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..2203
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.864"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 gccctgcggg gctgaaagaa aaccacaaaa aggcattacg gcagaaatgg cgatttatca
       61 tctcagcatg aaaatcatct ccagaaacag cggctacagc gccgttgctt ctgctgccta
      121 tcgttccggt tcactgatgc ttgatgagcg taccggactg actcacgact acacccgcaa
      181 aagcggtgtg gctgaagctg tgattctgac tcccgcgaca gcccctgcct ggtgtacgaa
      241 ccgtgccgaa ctctggaatg ccgttgagaa ggccgaacgc cggaagaact cacaacttgc
      301 gcgggaaatt gaactcgcca tcccccgcga actaccgcag gatgccgcac gggaaaccgt
      361 tctcgccttt gtcagagaaa actttgtcag tcagggcatg attgctgatg tggcgttcca
      421 tcacatggat aaaacaaatc cccatgcgca catcatgctg accacgagag ctgtcggacc
      481 tgctggattc ggtggaaagg tcagggactg gaacgaccgg acacatgccg agacgtggcg
      541 tgcatcatgg gctgaccatg cgaacagggc actggcgaac gccggttatc aggaagagat
      601 agaccatcgt tcatacgagc gacaggggct tgagaaaacg cctggtattc acctcggaaa
      661 gtctgcctgt gcgatggaaa cacgcggtat cgaaacagaa cgcggcgaac agaatcgtct
      721 gattaacaga cttaacctgg aaatacagat ttcccgtacc gaactcagaa acagcgggct
      781 gacagagcct gcccgccgtg tggctgacct tctgaatatc gtgcttcctg acaacgcaac
      841 ttcgtacaca ctacgtgaca tcatcgaagc gttgccgaag gacagtaatg cggcatggaa
      901 actcaccagt aatgccatgt cgatgatggc agatatgcag gcgacgcgca gacgctggga
      961 agagctgaac accgaacgaa aggaagcgat gagccatgct gccagcctga aaaaatcatc
     1021 gccgttttcg tccggttttt cccgtattcc cctgatgcaa tgggcggcac cggaataccg
     1081 tagcgaacag ggaaaaatcc agtcgctgag ccagcaaatg gaaaaattgc gacagcatta
     1141 ccggaaggtg gagaaacagg acattcccgc gagtcagacg gcatttgaaa aacagtggca
     1201 gcagtggggg gcatccggcc ttgcagctct cagagtccag ttgcttaaac gtgaagagga
     1261 attgcgccgg aaggagcagg aggatacaga acgtagtcgc gcggagcgac gagcggaaca
     1321 gctcaggaaa aatgacaatg ccgttatgga ttcaggggta ctgagcgcag tcgttacggg
     1381 gtatggcgag gcccccatgc ctggtaacgg ggaaatgacc tgttaccttc tgcttcacaa
     1441 ccggaacgga gaatacacgc tctgggggaa cgagctggag aaataccgca ccagaatctt
     1501 tgagcgtgtt gatttaatgc gtgacaggag tggttatatc tgtgaccgaa gcgaaatcgg
     1561 tcaacatcca ccactgcaac gtgtttattc ctctgcgacc tttgagcaac ttctgacgca
     1621 ggtttgccag acatggccgc aatatacccg caatctccgc cagccgaaga catggccgga
     1681 gtctttctgt cttggagaag accgccagcc agccatgcca tcactggcag caaggaaggt
     1741 ggattttacg cagagacgtc tgctaccgac gctgatgccg gtaatgagtt ctgtcgaccg
     1801 ggaaacccgc cagcttcagc ttttgctggt catgggcgtg gatgactctc tgggcggtgt
     1861 tgtccggctt aatggtacgc tgtatcccgc gtttgccgtt ccctcagccg ataacagcca
     1921 gctggtcatc agtgcactga ccgataaggg gttacggtat gcagggtatg gtgttgccgt
     1981 aaatcacgat gcagacagcc acatatcacc tgcgccggaa cttatggagt ttcatctgaa
     2041 aacccgtgag gcaccgcttt ttgctgccgt caacacacca gaaaaacagc cggatcatct
     2101 gttccgctcg ctgggtttta atcgtacctg ggacgaatgg cggcgcgagg aagatgcacg
     2161 aacacacgcc accgagagga gacatgatcg cggttggtca cagtgaatgt gaacaatatt
     2221 ttgttttgtt taacatcgtg ttatgttcgt cgatgttctt cgatgttcat cgacagggat
     2281 gaatgcaaga agcacacaca taatgcaagc atagttttgt ttttccgccc ccaaaaaatg
     2341 ggggttcttt tttcgctgca ttagagtgct tttgacgcaa tttttcactt caatagtacc
     2401 ttcgcactac taacacaaaa aaaacgcagc agggagcgtt ttaggtgcct taaaatcgat
     2461 tttagatcgt catcttgatt tgtttgtctt ccttgaagcg aaaatgcagg gctacaaccg
     2521 tgcgcccctt tttgatcgtc tcaaccgtta cagccaggtc gctttttttg ttcagttcgt
     2581 caatgcaggg tcttaataat tgctggttaa gtgatttata tgtttgatat ttatgctcta
     2641 tatcaagcat tgagcgaaag tcatcgagag cgataatacg ttcgccagta ctgcggaact
     2701 gctggaggag ttcgtatatc cttatactgt aacttcttga taatctagat atattcttga
     2761 ttacaactct agtaaattgc ccttgtagtt gtgtcaaata aggcattact gcgtcagaaa
     2821 atgttatttg tgctctggcc tcgcctttag cgtactgtgc ccgatactgg atccagcgga
     2881 actcctcacg tttctcgtca tccttgagaa tgattgacct gtcccacaaa cgatcaattg
     2941 cttcctgtaa ctgaacctcg gcatttttcc gccccatgtc agggaatgaa tcgaaatatt
     3001 ccgcagctgt aatggtcatt gtcttttgca attttggaga ttctgcatca gccccagact
     3061 tcaatcgacc gatgcaaagt aacaaaaatc gctgttcctg tagggtcaat ttataactgg
     3121 cttcaaccag cgcattggat ttataggcaa ttagatccga catagagcat ccctccccaa
     3181 aaggtaatga ataaaagata aacataccct caacgaaagg caatgccttt ttattttaat
     3241 atcccttttt ctgtgaatat taccttaaat catattgcct ttaccttaaa ttcccccacc
     3301 taaacacctt aaatcatatt acctttacct taaatcacat tacctatagc attgtaacat
     3361 tttgttttct ataaggaatt ttggcgctaa aacaagtatt aaaaacaaga tcttaaaaac
     3421 aagatctaaa aacttaacga cgagcctgtg gataacttgg agtgctgaaa acaaaaaacc
     3481 aataaaaaca atgcgttgca aaaaagcaga attgcaagtt ccgaatacac cccctccaac
     3541 cctgtggaaa tccagtattc atgcgggttc agcgcatatc acctcccgaa aaaccgtgcg
     3601 tatgtgctca atcaggcgac ggattcactt ccgcttcctc gcccactcgc tcgctaacgc
     3661 tcggtcgctg ggactgcggc gagcggacga tacaatcact gtcctggcag cgcttccagc
     3721 tcgttaatca gcggcagtat gcgcgtcaca tcagcctgtc gtgtcacctc tgccttcagg
     3781 aactggagta accaggcacg caactgcacc tcctccggct ttctgacctt tgccagcaat
     3841 gctgcaccca caatgacctt ctgcgctgtg tccagactcc tgctcttcgc cttcaggcgc
     3901 tgtaatctgg cctcagcatc agcaatcttc tgttcgagtg ttctgctcat gatgtaactc
     3961 cgtgcgcgat gaaaaatcgc attttagcgc gtcactggta gtttaaaaac tgagctggca
     4021 taatgcacgg aaaatcacga agtgcgcact tatacaatct ccactccgtt tcgattgtgt
     4081 gc
//
