LOCUS       CP088814                5164 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0262 plasmid pB16EC0262-2, complete
            sequence.
ACCESSION   CP088814
VERSION     CP088814.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391557
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:25:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,091
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,766
            CDSs (with protein)               :: 4,766
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 76 of 201
            Pseudo Genes (incomplete)         :: 147 of 201
            Pseudo Genes (internal stop)      :: 42 of 201
            Pseudo Genes (multiple problems)  :: 55 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0262"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0262-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            1057..1971
                     /locus_tag="LQQ73_25375"
     CDS             1057..1971
                     /locus_tag="LQQ73_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGJ75997.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(2175..2573)
                     /locus_tag="LQQ73_25380"
     CDS             complement(2175..2573)
                     /locus_tag="LQQ73_25380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ75998.1"
                     /translation="MRISNIEWLKKRIRFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            2750..3676
                     /locus_tag="LQQ73_25385"
     CDS             2750..3676
                     /locus_tag="LQQ73_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGJ75994.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3679..4212
                     /locus_tag="LQQ73_25390"
     CDS             3679..4212
                     /locus_tag="LQQ73_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ75995.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     GNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4278..4487)
                     /locus_tag="LQQ73_25395"
     CDS             complement(4278..4487)
                     /locus_tag="LQQ73_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ75996.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          1058..1971
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2751..3673
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.675"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctaatggaac tatagagtgg aggttgttac agtcgctttt ataaaacagc aaccagtttt
       61 tttcaatagt ctgatgatgg ttgtgacagt tttgtgatat tgttcaaaat gtttcttgtt
      121 gaatgtttgt tttttggtct attggtgtct ggttttattt tttctgctat tacatatcaa
      181 gaaatgtgat tggtgtagca agagtggaat gcgatgtgga taaactaatt ggttgtcgaa
      241 tctaatttac ttagaaaact acacttgttt ttgtgtatga atttagtgaa tttgtctgtc
      301 tgtgtgtttt cgtgcattta taaatccttc gtcaggatct taaatttagt gttgaatatg
      361 tagggcctgt acggtaatta ttatttagcg tagctacttc atcgttgttc gaacacaacc
      421 aacaacctgt aattgttgtt tctcttggtt ccccactttt gtggggtttt ttatctcttc
      481 cgtgcatcgt aaagcgcgga ctccttctgg aaaggttgtt ttagggcatc actgaggcat
      541 tttttgcgtt tgtgaatatt gagcatccga taactaaaat atttcccata gactctgatt
      601 cttgccaccg accccaggtc ggtttttttt tgtcctggcg agatgtgaag tttggaaaca
      661 ggaaaccccg tcagagcgcc acgcaccagg ggagaaaact tgtcgttttg agccgttcgg
      721 tgttcagaac gcacgaaacc gatcgcacat cgctttcgtg catagttatg cagccctcta
      781 aaaacgattc tggcgcgttt ttctggtttg gcctggtgtt ttcttgtctt tttgcgtttt
      841 ttgcgccaga aagcgactga gggcgtttta aggggtacgt acaacgggag ttatggtaaa
      901 tggatcggtt tttcgggaag gatcgacagg atttgccgtt gggtgtagtg taagcgactg
      961 aaaaacaaac gcccctgaaa ttcgtgtcta tccgccaaga ctttcacgaa aatacagggg
     1021 cgatgtcggt cgataacctg gaaggaatcg taacacatga gcgccgcgct tcaatacttc
     1081 gaagaaaatt taccccaccg tccctatcac acggatgatc tcgctttcgg ccttcgtatc
     1141 tccggcaaag ggcgcgcgct tcttgcacgg tatatccagc agaaccagcc tcatgcacag
     1201 ttctggctgg tttttgacgt tgaccgcgag ggggctgcga ttgactggag cgaccggaac
     1261 gctcccgcgc cgaacatcac cgttaaaaat cctgtgaacg gacacgctca cctgctctac
     1321 gcactcaaca tcgccgtgag aaccgcgcct gatgcctcgg ttaaggcgct gaaatatgcc
     1381 gcagcggttg aacgttcgct gtgtgagaaa ctttgtgcgg atgtgaacta cagcggcctg
     1441 atttgcaaaa atccgttcca cctggaatgg ctggtgatgg agtggcgcga ggaagcctat
     1501 accctcgatg aactggctga ttatctcgat ttgagcgcct cagagcgtcg tagcatcgat
     1561 aaacattacg ggatggggcg aaactgccac ctgttcgaaa tgacgcgcaa atgggcttac
     1621 agggcgattc gtcagggctg gccagcgttc tcacagtggc ttgaagcggt gatccagcgt
     1681 gtcgaaatgt acaatgcatc gcttcccgtt ccgctttcac tggctgaatg tcgggctatt
     1741 ggcaagagca ttgcgaaata cacgcacagg aacttcacgc cggaaacttt cgcacagtat
     1801 gtggctgata cgcacacgcc agaaattcag gctgcacgcg gtcgcaaggg cggttctaag
     1861 tctaagcgca gcacagtagc tacatctgca cgcacgctga aaccgtggga ggcattaggg
     1921 atcagtcgcg cctggtatta ccaactgaaa aaacgaggtc tcgtagagta gaccaaataa
     1981 gcctatatca gataacagcg cctttctggc gtctttttga gcagcaggtt ttttgccgtt
     2041 tttccccgtt cagccctgca atggcgcggc tttccgcgtg attgtggtgg tagtgctcgc
     2101 cgcagtctca tgaccgagcg tagcgagcga atgagcgagg aagcaccagg gaatcggaac
     2161 acggatggag gcacttacgc gtctgggttt ggggatgtct ccagatcgta cttaagttcc
     2221 ttaagtgctt gtaatatcct gtctttttta tagcatagct cgcctgtttt tgtgtctacc
     2281 agtcctgctt cgatcagtag cccagccgcg agaaagagcc ggtgattgcg ttcccgtcgt
     2341 tgtttttttc cctcgattcg tttttggacc gcctgttttc gttcgctctc ctgtgcctgt
     2401 aagtcgtttt tttccgctgt tgccagtacg tggagaagtt tgcgttcctg ttctgtgagt
     2461 ccggcttcgt tgtcgatcag gtcaataatc tgtctctgtc gtgcggtctg ttctccgagc
     2521 ttcctgatga atctgatccg tttcttcagc cattcgatgt tgctgatgcg catgttcgtg
     2581 atgctccctg gtgttttttt gcggtgtgtt gtggtcgtgc agtatatcgc ttgcggtcgg
     2641 cgggtgcagg gggtatcttc cgtgctaatt cgtaaaatta gaaggaagcg cacttatatg
     2701 tttctctcct tcgtcgagaa acagtgcgct cctgcagagg gctgaaaccg tggcaaccta
     2761 tcacctcagc gttaaatttg gtggcaaggg gcaggctgca aaccacgccg attacatcga
     2821 gcgcaaagag aaataccgcg accgtcagga tttggaatat tccgcacacg gaaacatgcc
     2881 ggaatgggcc agggataatc cttcccattt ctggcaggcg gctgaccagt ttgaacgtgc
     2941 gaacggttcg acctataggg aacttgaaat tgcattaccc cgtgaactga caccggaaca
     3001 gcgccttgaa ctggtgcagg attttgtacg tcaggaagct ggcgaacggc acgcctggag
     3061 ttttgccatt cacaatccga aagcgagcat tgacggcggc gaacagcctc atgcgcatat
     3121 catgatgagt cagcgtgtga atgatggcat tgaacgtaca ccggaacagt atttccggcg
     3181 ctataacgcc aggtatcccg accggggagg tgcgaaaaag gattccggaa acctgacccc
     3241 cactcagcaa aaagagcagt tgcgggaatt gcgaaaacgc tgggaggtga agcacaacga
     3301 acatatgaga aagcatggct ttgaaagggg cttcattgac tgccggacgc tgaaagaaca
     3361 gggcattgaa cgacgtcccg aagtgcatct gggttttgaa gctgctggac ggcttgatga
     3421 tgcccagcgt catgacatca tgcaaaaacg gtctgaaccg gactattccc gccatcttga
     3481 acaggcgcga acggtcgggc atgacatcag ggaatggctg gacactccgg ttaagcaaca
     3541 tgaactggtc agaaggcttc aacatgatgt tgcgcgggag catggctttg acatgagaga
     3601 cagagtgagc agacaggacc gaactgaacc tgaacgggtt aaatcaccgt cacgtggatt
     3661 ttctatggga ttctgaacat ggccagcaca gaagaaaaat tactcacgct cctgttatcg
     3721 gtggagcatc tgcaacatac cgcaatggag caacagaaag ctgttgctga ttctctggct
     3781 gtggcaaggg aaacacttcg cggatatcgg gatatttctg acggtgtggc aaaacaggtc
     3841 agggatgaag tcagaaatga ggtgcagaag ctggacgtgg caggactgat aggtgagcgc
     3901 atcagccagg attttcagcg gctggaagag tcagtcgcaa acatgaaggc aaacgcggat
     3961 gccctgaaca gggatattgc gacaattcac ggcaatatcg tgcaggaata caacaccctt
     4021 aaagggtata gctggaaatg gctgggtggg gtgtcagtgg cttttctggt tatgttctgt
     4081 gtgctcggat ggggaatgaa aacaatactc gacaggaact acaacgccac agcggcggtg
     4141 tatcagaaag ttgtgacact ggaagataaa ctggtggaga aacctgtcac ggggaagagc
     4201 cggaaaaact gacgcgcttc gcttgtccaa cgcctcacga cccgcgaaag gatttcgcga
     4261 accgttcacc gtttttttca atatgattct gtctgctttg gtcgataaag cagtcgatag
     4321 tattccagcc ttcgtatata taacggtacg tgctcatcag taagagctgg cggtattgtg
     4381 ttatcagggg gaagtttttt cagatactca cgaaatgcct ggcttgatgc cataaaatct
     4441 acagtagcat cgataatatg tggggtgttt tcacctaatt ttgccatgat gataatcctt
     4501 atattatcac ggtttgtgaa tgatagggtg tcactattat ttatgcgtta gtgaaccaga
     4561 aaaaatcccc cgttggcata gggggatatg taaggagaaa taacttcgca ggtgttcttg
     4621 tttgtataac gtgaaaagac tgcacgcaac ttaataagtc tgccgtactt actttcagtt
     4681 ttcaacataa ggtttataac aatgtatgga atggaagtga gctttaaggt ttcagctcta
     4741 aaaaataaaa tatttattag ttggctggtt gttgttcttg ttctgtatgt atataaacac
     4801 tatcgacata ttatgaccgg gaacattcct taccccctct gcgctcgcag aggttttttt
     4861 tgtcattaat taataatgaa ctgaaatgca gttccgatac ctccatttgc acattatccc
     4921 tgacatatgg taccggaacc gcattaattt tatgttataa gagtttattt ttaatgtcca
     4981 gatggtttaa tagtatttat atgtaattag ctgtatggat agtactggtt ttattgtata
     5041 acttgtcgtt ttttttattg tttttgtgtg gtgtgctaat ctttaagtta taaaaaaacc
     5101 ccttggaata caggggcaaa agtaaggaaa acaaatggat gctcagtttg tgtgacactt
     5161 taaa
//
