LOCUS       CP088783                4237 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0698 plasmid pB16EC0698-2, complete
            sequence.
ACCESSION   CP088783
VERSION     CP088783.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391565
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:48:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,101
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,797
            CDSs (with protein)               :: 4,797
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 79 of 187
            Pseudo Genes (incomplete)         :: 131 of 187
            Pseudo Genes (internal stop)      :: 40 of 187
            Pseudo Genes (multiple problems)  :: 51 of 187
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0698"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0698-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4087..4237,1..365))
                     /locus_tag="LQT75_24560"
     CDS             complement(join(4087..4237,1..365))
                     /locus_tag="LQT75_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="UGJ27543.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNTG
                     EKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQVLL
                     DSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDRIAF
                     TTLIERDC"
                     /name="replication/maintenance protein RepL"
     gene            complement(1552..2265)
                     /locus_tag="LQT75_24565"
     CDS             complement(1552..2265)
                     /locus_tag="LQT75_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="UGJ27544.1"
                     /translation="MNKKLIFSVLLCLFSFGSVAGIPVAIDASPEWAVEATRWTERLKQ
                     WSETARHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELANIYKQGNSYISDFTSN
                     PEGALSSQAKNLFSKYGAFNMCSTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAMS
                     QIQSLSNRIESSKDMKESQDLANALQAQSLKIQAIKMQYDVWDSKNRADAEMLKEQKYA
                     EYRLRQKNAKIPSFE"
                     /name="type IV secretion system protein"
     gene            complement(2262..3260)
                     /locus_tag="LQT75_24570"
     CDS             complement(2262..3260)
                     /locus_tag="LQT75_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="UGJ27540.1"
                     /translation="MSGFFEKFNSEVVSKIEEMTKPLQSQLGQDMLSLLSAGISVYILW
                     VGYQILAGKRQTPLQDLVWDLSKFAIILTFVSNADGYLTAAMDAIKSMKDGFLGKPVWA
                     TLDTMWSTTQLLADKVYAMDQSKYVSVEGGIGSMLVWGGSILIMSVAAVIYMLADVTMQ
                     LAILVAPIFIGCYMFGFTRVMFNNWLGILFSSVFTVIFAGVLVKIGTEFQTDMLSQISR
                     DANSTNILTMGAMAFVIGVLIAVFVWKSSSFAQQLAGAGVEGTLQGMALLGIGAAGMAV
                     EKTRNQMKRGTRTAAEWAGEKGEQKRASIGALGMEARRRASLEKARARNSA"
                     /name="type IV secretion system protein"
     gene            complement(3265..3528)
                     /locus_tag="LQT75_24575"
     CDS             complement(3265..3528)
                     /locus_tag="LQT75_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="UGJ27541.1"
                     /translation="MKKICTLLMSIFILSACGEDTKSSDWWLNHPKEATEKYKECKKSG
                     EDSVNCQNVKKVAGIIGRTYGPMLEILKAESAEYDKQHGLNR"
                     /name="EexN family lipoprotein"
     gene            complement(3616..3906)
                     /locus_tag="LQT75_24580"
     CDS             complement(3616..3906)
                     /locus_tag="LQT75_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="UGJ27542.1"
                     /translation="MTKHDAIRISQLHQIFYDDDGLIDSEKNVTILYSIGFTVDEIAHF
                     RNSTPNTVAAQLLNARVKLGCASVSSLKPMILLRLLLNIKEIRFGFETDCK"
                     /name="transcriptional regulator"
     Typing          complement(2458..3260)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="89.552"
                     /coverage="91.0"
                     /evalue="1.15e-160"
ORIGIN
        1 ctaagtttta agctatcata gctttttaaa aagtcatcaa gagcccttga atccaaaaga
       61 acctgatcct ttgcaaaggc ttgtttttga acagcccata aaagaacatt aaaagcttta
      121 attcctgccg atgataaatc aaacgttaac ccaatattcg cagtaaacaa cttaacaaat
      181 tgctcactat ctacacgctt aaaagtggtt acatgtgttc caaacttttc tcccgtgttt
      241 tgattaacca aaacattgtc atcccgccca agacgagata cttgtatccg tttccctttc
      301 atcgggatcg tcataagctc taaaaacggg tttgtttcgt atctctgtaa gcttagcgag
      361 ctcattattg catccttttt tgggctttgg agattcacaa taaatcctca taagctcaaa
      421 atcaagcaat ttttgagcct gcaaggtacc ggatctgagc ttacaaggta cagatcctga
      481 tcaccacttt ctaggattca tgcgggttcc gagaatttcc cttcttagtt cttaactaag
      541 gagcgtaggt gctcaattat ggctatcaat tctcttccgc ttcctcgccc actcgctcac
      601 tacgttcggt cgctggggct gcggcgagcg ggatcacctc tcgcatgttg tcagaaatgc
      661 cgttgctgtt ctgttcgaaa atgcgttctg tgggccatcc agagcaccag aacgcgattt
      721 tcaggtgttg gtaatgtttt agtcgtctta gctcttaaaa cgcgctcaga cgcacgaaac
      781 aggctttaga tgcccgtcag ggcatggaag gcgcgaaaaa tgcttacaga gggtttcagg
      841 aggtcaggcg cgccgatacc cctaaccagg ggctttcagg tcgccaggaa ccgcgccagc
      901 cccctgcaaa gcccattagc acggaacagg tagccacacg gttggtaggt ggtcgtagac
      961 cgtcgatgtt acagcgatgg gggtctgggg ttctccccag acggtttccg gttgcgctca
     1021 aggcgctgcc ggaaccgtgg cggctcggtg gttctagaga tcgggaaaca aaaacacgct
     1081 agaggttttg aggtcggcaa aagagcaatg caggatagac aatccgatgt caaatgttaa
     1141 catttgaatc agattgtcca aaaacgatgt ttttgcctgt gcctgccgca ggctctatat
     1201 ttagaattta gaatagaaaa ggccatctat tcgatgacct ttatttttta cttcattgaa
     1261 aatcgcatta ctattaataa cagaagtaaa atacaaaaca gctttaacgt aaaaaggagt
     1321 ctttctttta taccaccgct gggaacaaac ctcttttcaa ttttatcagt gataaattgc
     1381 aatgtaccat aatacattaa agcattaacc agctttaacg taagctccac acttaaaaca
     1441 ataactatga ttggtagcga gattaacagt ggcggcggtg tagaaccggt actattcaat
     1501 ctagaccaat gattatctac ccattctttt agcttattta gctttatcat atcattcgaa
     1561 actcggtatc tttgcatttt tctgacgcag gcgatactca gcatactttt gctctttcaa
     1621 catttccgca tcagccctgt ttttactatc ccatacatca tactgcattt taattgcctg
     1681 tatttttaac gactgcgcct gcaatgcgtt agccaaatcc tgggattctt tcatatcctt
     1741 tgaagactca attcggttac ttaaactctg aatctgagac attgcacttg atagctgctt
     1801 atttatctca tttccctgtt caatgctagc ggccgttgaa actattctgg ccttacaaag
     1861 gttatcagaa ctttcaatgc ctgtagaaca catattaaaa gcaccatact tgctgaacag
     1921 atttttcgcc tgactactta atgcaccttc aggattactg gtaaaatcag aaatataact
     1981 gttcccctgc ttataaatgt ttgccagctc tgattgtagg ttacttagtt catttgtgaa
     2041 tgccgcaata ttccttatac ctgttgccgt ggctaactgg tctttatatg cctgaatctg
     2101 actttgataa tgccgcgctg tttcagacca ttgtttgagt cgttctgtcc atcttgtggc
     2161 ttcaacagcc cattccggac tggcatcaat agcaaccgga atacctgcga cagaaccaaa
     2221 tgaaaataag cacaaaagta ctgaaaaaat tagttttttg ttcatgctga attcctcgca
     2281 cgtgcttttt ccagggatgc tcgtcttctt gcttccattc ccaatgcgcc tattgaagcc
     2341 cgtttctgct cccctttttc acctgcccat tcggcggctg ttcgcgttcc acgtttcatc
     2401 tggtttctcg ttttctctac ggccattcca gcagccccaa tccctaaaag agccattccc
     2461 tgaagagtac cttcaacacc tgcaccggca agctgctgcg cgaaactcga tgatttccag
     2521 acaaagacag caatcagaac tccaattaca aatgccattg cgcccattgt taaaatattt
     2581 gttgagttag catctctcga tatctgacta agcatatcag tttgaaactc ggtaccaatt
     2641 ttgacgagaa ctccagcaaa aataacagta aatacactag aaaataatat accaagccag
     2701 ttattaaaca ttactctagt gaatccaaac atataacaac cgataaatat cggagctaca
     2761 agaatagcta actgcattgt tacatcggca agcatgtaaa taacagccgc tacagacata
     2821 atcaggatac ttcctcccca aaccaacatt gacccgattc caccttcaac tgacacatat
     2881 ttggattgat ccatagcata aactttgtca gccaaaagct gagttgtact ccacatggta
     2941 tctagcgttg cccatacagg tttaccaagg aacccatcct tcatagattt aatagcatcc
     3001 attgctgccg tcagatagcc atcagcattg cttacgaatg ttaaaataat ggcaaatttt
     3061 gatagatccc agacgagatc ttgtaatggg gtctggcgtt tccctgccaa tatttgatag
     3121 ccaacccaga ggatataaac gctaatacct gcacttagaa gagataacat atcctgacct
     3181 aattgacttt gaagtggttt ggtcatctct tcgattttac taacaacttc gctattaaac
     3241 ttttcaaaga aaccagacat gttatcacct gttcaatcca tgttgcttat catattcagc
     3301 tgactcagcc ttaagtattt caagcatagg gccataggtt ctaccaataa ttccggcaac
     3361 ttttttaaca ttttgacaat taacagaatc ttcccctgac tttttacact ccttatattt
     3421 ctccgtcgct tctttgggat gattaagcca ccaatctgat gattttgtat cttcaccaca
     3481 tgcggaaaga atgaaaatag acattaagag cgtacaaatt tttttcatct ttgattcctc
     3541 gtatggacat tacaattgct gatacaatca gcaattgtaa ttctagtcaa gagcaaaaat
     3601 acctattgaa ctatattatt tgcaatcagt ttcgaatcca aaacgaatct cttttatgtt
     3661 caaaagcaat cttaataaga tcattggctt taaagatgaa accgaggcgc aacctaattt
     3721 aacccttgca tttaatagct gagccgcaac tgtgtttggt gttgaatttc taaaatgagc
     3781 tatttcatca actgtaaatc ctatactgta taaaatagtc acgttctttt ctgaatcaat
     3841 taatccatca tcatcgtaaa aaatttgatg aagttgagaa atgcgtatag catcatgttt
     3901 agtcataaaa acctctagcc aggttgttgt gattagcagg cgaacaaaac accacttttg
     3961 ttcgcctgcg actacttcta gtagcctgcg gcaggcacag gcaaaaacat cgtttttgga
     4021 caatcatgga taaaatgtta acattttgac catgattgtc tatcctgcat tgtattgttc
     4081 tatcgattaa caatccctct caattagggt tgtaaatgct attcgatctc cattaaatac
     4141 aaaattgggg tttataaaat aaaatccttt acgcattgtc tttgctataa tctgagcttt
     4201 ttctaactcg ttaattcctc tctttaacgt agcataa
//
