LOCUS       CP088781                5093 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0698 plasmid pB16EC0698-1, complete
            sequence.
ACCESSION   CP088781
VERSION     CP088781.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391565
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:48:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,101
            CDSs (total)                      :: 4,984
            Genes (coding)                    :: 4,797
            CDSs (with protein)               :: 4,797
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 79 of 187
            Pseudo Genes (incomplete)         :: 131 of 187
            Pseudo Genes (internal stop)      :: 40 of 187
            Pseudo Genes (multiple problems)  :: 51 of 187
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0698"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0698-1"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            72..2231
                     /locus_tag="LQT75_00005"
     CDS             72..2231
                     /locus_tag="LQT75_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGJ22901.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAEA
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVANLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWAASGLAALRAQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNWEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPTADNSQLVINALTDKG
                     LRFAGYGEAVNHDADSPARPAPELMLFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWQREEYARTRVSDRKHERGWLL"
                     /name="MobA/MobL family protein"
     gene            2484..2975
                     /locus_tag="LQT75_00010"
     CDS             2484..2975
                     /locus_tag="LQT75_00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGJ22900.1"
                     /translation="MAIGDDAIRDAFYLFTQQVAEMDNESLPKDVWGTPCFTYLMTRKQ
                     FNQMKVICQRNGWDVPTSPAIPITWSMFKHVLSARKSKDKLSWQECAEILATAFSVQSN
                     VYVSRDYSEQTIVLNASCRISVAGAGFFAMAIIDVSENNLAPVTAYHVTEAKCKAISRG
                     "
                     /name="hypothetical protein"
     gene            complement(3508..4200)
                     /locus_tag="LQT75_00015"
     CDS             complement(3508..4200)
                     /locus_tag="LQT75_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGJ22898.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYELLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCITELNKKSDLVVTVETIKKGRTVAALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(4732..4986)
                     /locus_tag="LQT75_00020"
     CDS             complement(4732..4986)
                     /locus_tag="LQT75_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UGJ22899.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLPFLRREVTRQADLNRIQPLIDELEALPER"
                     /name="mobilization protein"
     Typing          72..430
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.333"
                     /coverage="100.0"
                     /evalue="8.460000000000002e-179"
     Typing          complement(4991..5093)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.038"
                     /coverage="98.0"
                     /evalue="3.58e-45"
     Typing          complement(3509..4200)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7..2222
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.482"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcttcactcc gtttcgattg tgtgcgccct gcggggctga aagaaaactg caaaaaggca
       61 ttacggcaga aatggcgatt tatcatctca gcatgaaaat catctccaga aacagcggct
      121 acagcgccgt cgcttctgct gcctatcgtt ccggttcact gatgcttgat gagcgtacag
      181 gactgactca tgactacacc cgcaaaagcg gtgtggctga agctgtgatt ctgacccccg
      241 cgacagcccc tgcctggtgt acgaaccgtg ccgaactctg gaatgccgtt gagaaagccg
      301 aacgccggaa gaactcacaa cttgcgcggg aaattgaact cgccatcccc cgcgaactgc
      361 cgcaggatgc cgcacgggaa accgttctcg cctttgtcag agaaaacttt gtcagtcagg
      421 gcatgattgc tgatgtggcg ttccatcaca tggataaaac aaatccccat gcgcacatca
      481 tgctgaccac gagggctgtc ggacctgctg gattcggcgg aaaggtcagg gactggaacg
      541 accggacaca tgctgaggcg tggcgggcat catgggctga ccatgcaaac agggcactgg
      601 cgaacgccgg ttatcaggaa gagatagacc atcgttcata cgagcgacag gggcttgaga
      661 aaacgccggg tattcacctc ggaaagtctg cctgtgcgat ggaaacacgt ggtatcgaaa
      721 cagaacgcgg cgaacagaat cgtctgatta acaggcttaa cctggaaata cagatttccc
      781 gcaccgaact caggaacggc gggctgacag agccagcccg ccgtgtggct aaccttctga
      841 atatcgggct tcctgacaac gcaacttcgt acacactacg tgacatcatc gaagcgttgc
      901 cgaaggacag taatgcggca tggaaactca ccagtaatgc catgtcgatg atggcagata
      961 tgcaggcgac gcgcagacgc tgggaagagc tgaacaccga acgaaaggaa gcgatgagcc
     1021 atgctgccag cctgaaaaaa tcatcgccgt tttcgtccgg tttttcccgt attcccctga
     1081 tgcaatgggc ggcaccggaa taccgtagtg agcagggaaa aatccagtct ctgagccagc
     1141 aaatggaaaa attgcgacag cattaccgga aggtggagaa acaggacatt cccgcgagtc
     1201 agacggcatt tgaaaaacag tggcagcagt gggccgcatc cggccttgca gctctcagag
     1261 cccagttgct taaacgtgaa gaggaattac gcaggaagga gcaggaggat acagaacgta
     1321 gtcgcgcgga gcgacgagcg gaacagctca ggaaaaatga caatgccgtt atggattcag
     1381 gggtactgag cgcagtcgtt acggggtatg gcgaggcccc tatgcctggt aactgggaaa
     1441 tgacctgtta ccttctgctt cacaaccgga acggagaata tacgctctgg gggaacgagt
     1501 tggagaaata ccgcaccagg atctttgagc gtgttgattt aatgcgtgac aggagtggtt
     1561 atatctgtga ccgaagcgaa atcggtcaac atccaccact gcaacgtgtt tattcctctg
     1621 cgacctttga gcaacttctg acgcaggttt gccagacatg gccgcaacat acccgcaatc
     1681 tccgccagcc gaagacatgg ccggagtctt tttgtcttgg agaagaccgc cagccagcca
     1741 tgccatcact ggcagcaagg aaggtggatt ttacgcaggg acgtctgcca ccgacgctga
     1801 tgccggtaat gagttctgtc gaccgggaaa cccgccagct tcagcttttg ctggtcatgg
     1861 gcgtggatga ctctctgggc ggtgttgtcc ggcttaatgg tacgctgtat cctgcgtttg
     1921 ccgtccccac agccgacaac agccagctgg taatcaatgc tctgaccgac aaaggtttac
     1981 gatttgccgg ttatggtgaa gcggtaaacc acgatgctga cagtccggca cgccctgcgc
     2041 cagaacttat gctgtttcat ctgaaaaccc gtgaggcacc gctttttgct gccgtcaata
     2101 caccagaaaa acagccggat catttgttcc gctcgctggg ttttaatcgc acctgggacg
     2161 aatggcagcg cgaggaatat gcacgaacac gcgtctccga caggaaacat gagcgcggct
     2221 ggttacttta aagcactttc cgcatacata ttttttatat gggcgaaaac agaataattc
     2281 gttttgagat agaaaatttc aactctagta ggtagcgcaa cgaccgtgat tcggcaaacg
     2341 ggagaaagac cgcgcaatag ccgtttcagg tatgataata aagcgattgc tcagacattg
     2401 aattatgctg agctgtcggc agtggaataa cgacgcgtga agaccgtcat aactaaaaaa
     2461 tgccggataa ataaggaaaa tcagtggcta ttggcgatga tgctatccgt gatgcttttt
     2521 atctttttac acagcaagtc gctgaaatgg ataatgaatc actccctaaa gacgtctggg
     2581 gaacaccttg tttcacttat ttgatgacta gaaaacaatt taaccaaatg aaggtcattt
     2641 gtcagaggaa tggttgggat gtgcccacat caccggcgat acctatcact tggagtatgt
     2701 tcaagcatgt gctctctgct cgaaaatcga aggataaatt aagctggcaa gaatgcgcag
     2761 aaatcttggc aactgctttt tctgtgcaga gtaatgtata cgttagcaga gattattcag
     2821 agcagacgat tgtacttaat gcttcttgtc gcatcagcgt tgcaggggca ggatttttcg
     2881 caatggcgat tattgatgtc tctgaaaata atttggcccc cgtaactgca tatcatgtga
     2941 cggaggccaa atgtaaagct atatcgcgag ggtgatagga aggacgccca tttaagcagc
     3001 tctcctcaat ttaatgagaa tgaaccataa cgcctaaata tttcccttca ttgtttcctt
     3061 tcggatctag cagcatcggc ggttttctgc tgtctatcaa atcgctaatt gttcaggaac
     3121 atcaatgctc ccactgccga acattagagg atttctccgt ttatggaatg ttcttgcagt
     3181 acattcaatc tacatcaatt gtggctatag tacaatattt attgagcgca gctaaggcta
     3241 aactccccgc gaacaggggc ttaagatagg gtgtggaatg tcgcattttg tgacttaggg
     3301 gcctcatctg cctcaattag ttttatccga ttggtactgg ttgttagcgt agagtaatga
     3361 ctatggtcca actggaaccc aaacgctaaa gatgctttaa agctctctaa agttattttt
     3421 acgcagtttt cccctctctt ccatcgccac cattaccaga tgtaaaaaaa tgcgccagcg
     3481 cgcgttttag atgccttaaa atcgatttta gatcgtcatc ttgatctgct tgtcttcctt
     3541 gaatcgaaaa tgcagtgctg caaccgtgcg ccccttcttg atcgtctcaa ccgtcacaac
     3601 cagatcgctt tttttattca gttccgtgat acagggtttt attagtatct tatttaagtc
     3661 tctgaattgt ttgtatttat tttcaatacc taacattgaa cgaaagtcat taagagcaat
     3721 aattctctct cccgtactgc ggaattgctg gagaagttcg tatattctta tactgtaact
     3781 gcttgataaa cctgatatgt tttttataac aacgcgcgtg aattgcccct tcagttgcgt
     3841 cagataaggc attaccgcgt cagagaaggt tattcgagcc ttggcctcac ctttagcgta
     3901 ctgtgcgcga tactggatcc agcggaactc ctcacgcttc tcgtcatcct tgagaatgat
     3961 cgacctgtcc cataaacgat cgatcgcttc ctgtaactga acctcggcat ttttccgccc
     4021 catgtcaggg aatgaatcaa aatattccgc ggcggtaatg gtcattgttt tttgcaattc
     4081 agcatcagca ccagatttca atcgactgat acacagcaac agaaaacgtt gttcctgtag
     4141 ggtcagtttg tagctggcct caactagagc attcgacttg taggcaatca agtccgacat
     4201 acaaagctcc cgttcaaatg tggtgatact ttaatgtgcc ccacaaataa gaaatgtgga
     4261 atattttttt ttatatcctc acatttctgt gaataacacc ttaaattcct ccacctttac
     4321 cttaaatccc tccacctaaa caccttaaat tcctccacct ttaccttaaa tccctccact
     4381 tatacaactg taagccattg attttaataa tgaatttcac ccctaaaaca agtattaaaa
     4441 acaagatctt aaaaacaaga tctaaaaact taacggcgag cctgtggaaa acttaggatg
     4501 ccaaaaagaa aaactcagta aaaacaatgc gttgcaaaaa agcagaattg caaattccga
     4561 atactccccc tccagccctg tggaaatcca gtattcatgc gggttcagcg cgtatcacct
     4621 gccgaaaaac cgtgcgtatg tgctcaacct ggctacgggt tcacttccgc ttcctcgctc
     4681 actcgctcgc taacgcttgg tcgctggagc tgcggcgagc ggacggtgta atcaccgttc
     4741 tggcagtgcc tccagctcgt caatcagtgg ctgtatcctg ttcaagtcag cctgccgtgt
     4801 aacttctctc cgcaggaacg ggagcaacca ggcccgcaac tgcacttcct ccggctttct
     4861 gacctttgcc agcaatgctg cacccacaac gaccttctgc gctgtgtcca gactcctgct
     4921 cttcgccttc aggcgctgca atctggcctc agcttcggca atcttctgtt cgagtgttct
     4981 gctcattccg taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca ctggcagttt
     5041 aaaaactgag ctggcataat gcacggaaaa tcacgaagtg cgcacttata caa
//
