LOCUS       CP088759                5168 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0899 plasmid pB16EC0899-5, complete
            sequence.
ACCESSION   CP088759
VERSION     CP088759.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391573
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 222x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:49:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,213
            CDSs (total)                      :: 5,094
            Genes (coding)                    :: 4,922
            CDSs (with protein)               :: 4,922
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 55 of 172
            Pseudo Genes (incomplete)         :: 122 of 172
            Pseudo Genes (internal stop)      :: 29 of 172
            Pseudo Genes (multiple problems)  :: 31 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0899"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0899-5"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(5052..5168,1..282)
                     /locus_tag="LQT62_26045"
     CDS             join(5052..5168,1..282)
                     /locus_tag="LQT62_26045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI88807.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHQLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(486..1400)
                     /locus_tag="LQT62_26050"
     CDS             complement(486..1400)
                     /locus_tag="LQT62_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGI88808.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            3138..3347
                     /locus_tag="LQT62_26055"
     CDS             3138..3347
                     /locus_tag="LQT62_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI88804.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(3413..3946)
                     /locus_tag="LQT62_26060"
     CDS             complement(3413..3946)
                     /locus_tag="LQT62_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI88805.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3949..4875)
                     /locus_tag="LQT62_26065"
     CDS             complement(3949..4875)
                     /locus_tag="LQT62_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGI88806.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(626..1400)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3953..4875)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acagaacagg aacgcaaact tctccacgta ctggcaacag cggaaaaaaa cgacttacag
       61 gcacaggaga gcgaacgaaa acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga
      121 cgggaacgca atcaccagct ctttctcgcg gctgggctac tgatcgaagc aggactggta
      181 gacacaaaaa caggcgagct atgctataaa aaagacagga tattacaagc acttaaggaa
      241 cttaagtacg atctggagac atccccaaac ccagacgcgt aagtgcctcc atccgtgttc
      301 cgattccctg gtgcttcctc gctcattcgc tcgctacgct cggtcatgag actgcggcga
      361 gcactaccac cacaatcacg cggaaagccg cgccattgca gggctgaacg gggaaaaacg
      421 gcaaaaaacc tgctgctcaa aaagacgcca gaaaggcgct gttatctgat ataggcttat
      481 ttggtctact ctacgagacc tcgttttttc agttggtaat accaggcgcg actgatccct
      541 aatgcctccc acggtttcag cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta
      601 gaaccgccct tgcgaccgcg tgcagcctga atttctggcg tgtgcgtatc agccacatac
      661 tgtgcgaaag tttccggcgt gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata
      721 gcccgacatt cagccagtga aagcggaacg ggaagcgatg cattgtacat ttcgacacgc
      781 tggatcaccg cttcaagcca ctgtgagaac gctggccagc cctgacgaat cgccctgtaa
      841 gcccatttgc gcgtcatttc gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg
      901 atgctacgac gcgctgaggc gctcaaatcg agataatcag ccagttcatc gagggtatag
      961 gcttcctcgc gccactccat caccagccat tccaggtgga acggattttt gcaaatcagg
     1021 ccgctgtagt tcacatccgc acaaagtttc tcacacagcg aacgttcaac cgctgcggca
     1081 tatttcagcg ccttaaccga ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag
     1141 agcaggtgag cgtgtccgtt cacaggattt ttaacggtga tgttcggcgc gggagcgttc
     1201 cggtcgctcc agtcaatcgc agccccctcg cggtcaacgt caaaaaccag ccagaactgc
     1261 gcatgaggct ggttctgctg tatgtaccgc gcaagaagcg cacgcccttt gccggagatg
     1321 cgaagaccaa aagcgagatc atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag
     1381 tattgaagcg cggcgctcat gtgttacgat tctctccggg ttatgcgacc tacacgccct
     1441 ctgtatttca tgttgaatct tgccggatct ggacatgatt tcaggggcgt ttgtttttca
     1501 gtcgcttaca ctacacccaa cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt
     1561 taccataact cccgttgtac gtacccatta aaacgccctc aatcgctttc tggcgcaaaa
     1621 aacgcaaaaa gacaagaaaa caccaggcca aaccagaaaa acgcgtcaga atcgttttca
     1681 gggcgttgca taactatgca cgaaagcgat gtgcgatcgg tttcgtacgt tctgaacacc
     1741 gaacggctca aaacgacaag ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg
     1801 tttccaaact tcacatctcg ccaggacaaa aaaaaaccga cctggggtcg gtggcaagaa
     1861 tcagagtcta tgggaaatat tttagttatc ggatgctcaa tattcacaaa cgcaaaaaat
     1921 gcctcagtga tgccctaaaa caacctttcc agaaggagtc cgcgctttac gatgcacgga
     1981 agagataaaa aaccccacaa aagtggggaa ccaagagaaa caacaattac aggttgttgg
     2041 ttgtgttcga acaacgatga agtagctacg ctaaataata attaccgtac aggccctaca
     2101 tattcaacac taaatttaag atcctgacga aggatttata aatgcacgaa aacacataga
     2161 cagacaaatt cactaaattc atacacaaaa acaagtgtag ttttctaagt aaattagatt
     2221 cgacaaccaa ttagtttatc cacatcgcat tccactcttg ctacaccaat cacatttctt
     2281 gatatgtaat agcagaaaaa ataaaaccag acaccaatag accaaaaaac aaacattcaa
     2341 caagaaacat tttgaacaat atcacaaaac tgtcacaacc atcatcagac tattgaaaaa
     2401 aactggttgc tgttttataa aagcgactgt aacaacctcc actctatagt tccattagtt
     2461 taaagtgtca cacaaactga gcatccattt gttttcctta cttttgcccc tgtattccaa
     2521 ggggtttttt tataacttaa agattagcac accacacaaa aacaataaaa aaaaaacgac
     2581 aagttataca ataaaaccag tactatccat acagctaatt acatataaat actattaaac
     2641 catctggaca ttaaaaataa actcttataa cataaaatta atgcggttcc ggtaccatat
     2701 gtcagggata atgtgcaaat ggaggtatcg gaactgcatt tcagttcatt attaattaat
     2761 gacaaaaaaa acctctgcga gcgcagaggg ggtaaggaat gttcccggtc ataatatgtc
     2821 gatagtgttt atatacatac agaacaagaa caacaaccag ccaactaata aatattttat
     2881 tttttagagc tgaaacctta aagctcactt ccattccata cattgttata aaccttatgt
     2941 tgaaaactga aagtaagtac ggcagactta ttaagttgcg tgcagtcttt tcacgttata
     3001 caaacaagaa cacctgcgaa gttatttctc cttacatatc cccctatgcc aacgggggat
     3061 tttttctggt tcactaacgc ataaataata gtgacaccct atcattcaca aaccgtgata
     3121 atataaggat tatcatcatg gcaaaattag gtgaaaacac cccacatatt atcgatgcta
     3181 ctgtagattt tatggcatca agccaggcat ttcgtgagta tctgaaaaaa cttccccctg
     3241 ataacacaat accgccagct cttactgatg agcacgtacc gttatatata cgaaggctgg
     3301 aatactatcg tctgctttat cgaccaaagc agacagaatc atattgaaaa aaacggtgaa
     3361 cggttcgcga aatcctttcg cgggtcgtga ggcgttggac aagcgaagcg cgtcagtttt
     3421 tccggctctt ccccgtgaca ggtttctcca ccagtttatc ttccagtgtc acaactttct
     3481 gatacaccgc cgctgtggcg ttgtagttcc tgtcgagtat tgttttcatt ccccatccga
     3541 gcacacagaa cataaccaga aaagccactg acaccccacc cagccatttc cagctatacc
     3601 ctttaagggt gttgtattcc tgcacgatat tgctgtgaat tgtcgcaata tccctgttca
     3661 gggcatccgc gtttgccttc atgtttgcga ctgactcttc cagccgctga aaatcctggc
     3721 tgatgcgctc acctatcagt cctgccacgt ccagcttctg cacctcattt ctgacttcat
     3781 ccctgacctg ttttgccaca ccgtcagaaa tatcccgata tccgcgaaga gtttcccttg
     3841 ccacagccag agaatcagca acagctttct gttgctccat tgcggtatgt tgcagatgct
     3901 ccaccgataa caggagcgtg agtaattttt cttctgtgct ggccatgttc agaatcccat
     3961 agaaaatcca cgtgacggtg atttaacccg ttcaggtgca gttcggttct gtctgctcac
     4021 tctgtctctc atgtcaaagc catgctcccg cgcaacatca tgttgcagcc ttctgaccag
     4081 ttcatgttgc ttaaccggag tgtccagcca ttccctgatg tcatgcccga ccgttcgcgc
     4141 ctgttcaaga tggcgggaat agtccggttc agaccgtttt tgcatgatgt catgacgctg
     4201 ggcatcatca agccgtccgg cagcttcaaa acccagatgc acttcgggac gtcgttcaat
     4261 gccctgttct ttcagcgtcc ggcagtcaat gaagcccctt tcaaagccat gctttctcat
     4321 atgttcgttg tgcttcacct cccagcgttt tcgcaattcc cgcaactgct ctttttgctg
     4381 agtgagagtc aggcttccgg aatccttttt cgcacctccc cgctcgggat acctggcgtt
     4441 atagcgccgg aaatactgtt ccggtgtacg ttcaatgcca tcattcacac gctgactcat
     4501 catgatatgc gcatgaggct gttcgccgcc gtcaatgctc gctttcggat tgtgaatggc
     4561 aaaactccag gcgtgccgtt cgccagcttc ctgacgtaca aaatcctgca ccagttcaag
     4621 gcgctgttcc ggtgtcagtt cacggggtaa tgcaatttca agttccctat aggtcgaacc
     4681 gttcgcacgt tcaaactggt cagccgcctg ccagaaatgg gaaggattat ccctggccca
     4741 ttccggcatg tttccgtgtg cggaatattc caaatcctga cggtcgcggt atttctcttt
     4801 gcgctcgatg taatcggcgt ggtttgcagc ctgccccttg ccaccaaatt taacgctgag
     4861 gtgataggtt gccacggttt cagccctctg caggagcgca ctgtttctcg acgaaggaga
     4921 gaaacatata agtgcgcttc cttctaattt tacgaattag cacggaagat accccctgca
     4981 cccgccgacc gcaagcgata tactgcacga ccacaacaca ccgcaaaaaa acaccaggga
     5041 gcatcacgaa catgcgcatc agcaacatcg aatggctgaa gaaacggatc ggattcatca
     5101 ggaagctcgg agaacagacc gcacgacaga gacagattat tgacctgatc gacaacgaag
     5161 ccggactc
//
