LOCUS       CP088758                5671 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0899 plasmid pB16EC0899-4, complete
            sequence.
ACCESSION   CP088758
VERSION     CP088758.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391573
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 222x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:49:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,213
            CDSs (total)                      :: 5,094
            Genes (coding)                    :: 4,922
            CDSs (with protein)               :: 4,922
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 55 of 172
            Pseudo Genes (incomplete)         :: 122 of 172
            Pseudo Genes (internal stop)      :: 29 of 172
            Pseudo Genes (multiple problems)  :: 31 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0899"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0899-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(5546..5671,1..2121))
                     /locus_tag="LQT62_26025"
     CDS             complement(join(5546..5671,1..2121))
                     /locus_tag="LQT62_26025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGI88802.1"
                     /translation="MENHEVRTYTISTPFRLCAPCGAERKPQKGITAEMAIYHLSMKII
                     SRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRKSGVAEAVILTPATAPAWCMNRAELW
                     NAVEKAERRKNSQLAREIELAIPRELPQDAARETVLAFVRENFVSQGMIADVAFHHMDK
                     TNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAETWRASWADHANRALANAGYQEEIDHR
                     SYERQGLEKTPGIHLGKSACAMETRGIETERGEQNRLINRLNLEIQISRTELRNSGLTE
                     PARRVADLLNIVLPDNATSYTLRDIIEALPKDSNAAWKLTSNAMSMMADMQATRRRWEE
                     LNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWAAPEYRSEQGKIQSLSQQMEKLRQHY
                     RKVEKQDIPASQTAFEKQWQQWGASGLAALRVQLLKREEELRRKEQEDTERSRAERRAE
                     QLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTR
                     IFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKT
                     WPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDS
                     LGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYAGYGVAVNHDADSHISPAPELM
                     EFHLKTREAPLFADLLDSVERSGTGTTGHMPRRGVHHGLTMRTGHWRTPVIRKR"
                     /name="MobA/MobL family protein"
     gene            2198..2452
                     /locus_tag="LQT62_26030"
     CDS             2198..2452
                     /locus_tag="LQT62_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UGI88803.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            2985..3686
                     /locus_tag="LQT62_26035"
     CDS             2985..3686
                     /locus_tag="LQT62_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGI88801.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3942..5588)
                     /locus_tag="LQT62_26040"
                     /pseudo=""
     CDS             complement(3942..5588)
                     /locus_tag="LQT62_26040"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     Typing          complement(1662..2020)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2021..2197
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          2986..3686
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..2106)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.715"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3946..5669)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcaaaaagc ggtgcctcac gggttttcag atgaaactcc ataagttccg gcgcaggtga
       61 tatgtggctg tctgcatcgt gatttacggc aacaccatac cctgcatacc gtaacccctt
      121 atcggtcagt gcactgatga ccagctggct gttatcggct gagggaacgg caaacgcggg
      181 atacagcgta ccattaagcc ggacaacacc gcccagagag tcatccacgc ccatgaccag
      241 caaaagctga agctggcggg tttcccggtc gacagaactc attaccggca tcagcgtcgg
      301 tagcagacgt ccctgcgtaa aatccacctt ccttgctgcc agtgatggca tggctggctg
      361 gcggtcttct ccaagacaga aagactccgg ccatgtcttc ggctggcgga gattgcgggt
      421 atattgcggc catgtctggc aaacctgcgt cagaagttgc tcaaaggtcg cagaggaata
      481 aacacgttgc agtggtggat gttgaccgat ttcgcttcgg tcacagatat aaccactcct
      541 gtcacgcatt aaatcaacac gctcaaagat tctggtgcgg tatttctcca gctcgttccc
      601 ccagagcgtg tattctccgt tccggttgtg aagcagaagg taacaggtca tttccccgtt
      661 accaggcatg ggggcctcgc cataccccgt aacgactgcg ctcagtaccc ctgaatccat
      721 aacggcattg tcatttttcc tgagctgttc cgctcgtcgc tccgcgcgac tacgttctgt
      781 atcctcctgc tccttccggc gcaattcctc ttcacgttta agcaactgga ctctgagagc
      841 tgcaaggccg gatgcccccc actgctgcca ctgtttttca aatgccgtct gactcgcggg
      901 aatgtcctgt ttctccacct tccggtaatg ctgtcgcaat ttttccattt gctggctcag
      961 cgactggatt tttccctgtt cgctacggta ttccggtgcc gcccattgca tcaggggaat
     1021 acgggaaaaa ccggacgaaa acggcgatga ttttttcagg ctggcagcat ggctcatcgc
     1081 ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc gtcgcctgca tatctgccat
     1141 catcgacatg gcattactgg tgagtttcca tgccgcatta ctgtccttcg gcaacgcttc
     1201 gatgatgtca cgtagtgtgt acgaagttgc gttgtcagga agcacgatat tcagaaggtc
     1261 agccacacgg cgggcaggct ctgtcagccc gctgtttctg agttcggtac gggaaatctg
     1321 tatttccagg ttaagtctgt taatcagacg attctgttcg ccgcgttctg tttcgatacc
     1381 gcgtgtttcc atcgcacagg cagactttcc gaggtgaata ccaggcgttt tctcaagccc
     1441 ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa ccggcgttcg ccagtgccct
     1501 gttcgcatgg tcagcccatg atgcacgcca cgtctcggca tgtgtccggt cgttccagtc
     1561 cctgaccttt ccaccgaatc cagcaggtcc gacagctctc gtggtcagca tgatgtgcgc
     1621 atggggattt gttttatcca tgtgatggaa cgccacatca gcaatcatgc cctgactgac
     1681 aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg gcatcctgcg gtagttcgcg
     1741 ggggatggcg agttcaattt cccgcgcaag ttgtgagttc ttccggcgtt cggccttctc
     1801 aacggcattc cagagttcgg cacggttcat acaccaggca ggggctgtcg cgggagtcag
     1861 aatcacagct tcagccacac cgcttttgcg ggtgtagtcg tgagtcagtc cggtacgctc
     1921 atcaagcatc agtgaaccgg aacgataggc agcagaagca acggcgctgt agccgctgtt
     1981 tctggagatg attttcatgc tgagatgata aatcgccatt tctgccgtaa tgcctttttg
     2041 tggttttctt tcagccccgc agggcgcaca caatcgaaac ggagtggaga ttgtataagt
     2101 gcgcacttcg tgattttcca tgcattatgc cagctcagtt tttaaactac cagtgacgcg
     2161 ctaaaatgcg atttttcatc gcgcacggag ttacatcatg agcagaacac tcgaacagaa
     2221 gattgctgat gctgaggcca gattacagcg cctgaaggcg aagagcagga gtctggacac
     2281 agcgcagaag gtcattgtgg gtgcagcatt gctggcaaag gtcagaaagc cggaggaggt
     2341 gcagttgcgt gcctggttac tccagttcct gaaggcagag gtgacacgac aggctgatgt
     2401 gacgcgcata ctgccgctga ttaacgagct ggaagcgctg ccaggacagt gattgtatcg
     2461 tccgctcgcc gcagtcccag cgaccgagcg ttagcgagcg agtgggcgag gaagcggaag
     2521 tgaatccgtc gcctgattga gcacatacgc acggtttttc gggaggtgat atgcgctgaa
     2581 cccgcatgaa tactggattt ccacagggtt ggagggggtg tattcggaac ttgcaattct
     2641 gcttttttgc aacgcattgt ttttattggt tttttgtttt cagcactcca agttatccac
     2701 aggctcgtcg ttaagttttt agatcttgtt tttaagatct tgtttttaat acttgtttta
     2761 gcgccaaaat tccttataga aaacaaaatg ttacaacgct ataggtaata tgatttaagg
     2821 taaaggtaat atgatttaag gtgtttaggt ggaggaattt aaggtaaagg caatatgatt
     2881 taaggtaata ttcacagaaa aagggatatt aaaataaaaa ggcattgcct ttcgttgagg
     2941 gtatgtttat cttttattca ttaccttttg gggagggatg ctctatgtcg gatctaattg
     3001 cctataaatc caatgcgctg gttgaagcca gttataaatt gaccctacag gaacagcgat
     3061 ttttgttact ttgcatcggt cgattgaagt ctggggctga tgcagaatct ccaaaattgc
     3121 aaaagacaat gaccattaca gctgcggaat atttcgattc attccctgac atggggcgga
     3181 aaaatgccga ggttcagtta caggaagcaa ttgatcgttt gtgggacagg tcaatcattc
     3241 tcaaggatga cgagaaacgt gaggagttcc gctggatcca gtatcgggca cagtacgcta
     3301 aaggcgaggc cagagcacaa ataacatttt ctgacgcagt aatgccttat ttgacacaac
     3361 tacaagggca atttactaga gttgtaatca agaatatatc tagattatca agaagttaca
     3421 gtataaggat atacgaactc ctccagcagt tccgcagtac tggcgaacgt attatcgctc
     3481 tcgatgattt tcgctcaatg cttgatatag agcataaata tcaaacatat aaatcactta
     3541 accagcaatt attaagaccc tgcattgacg aactgaacaa aaaaagcgac ctggctgtaa
     3601 cggttgagac gatcaaaaag gggcgcacgg ttgtagccct gcattttcgc ttcaaggaag
     3661 acaaacaaat caagatgacg atctaaaatc gattttaagg cacctaaaac gctccctgct
     3721 gcgttttttt tgtgttagta gtgcgaaggt actattgaag tgaaaaattg cgtcaaaagc
     3781 gctctaatgc agcgaaaaaa gaacccccat tttttggggg cggaaaaaca aaactatgct
     3841 tgcattatgt gtgtgcttct tgcattcatc cctgtcgatg aacatcgaag aacatcgacg
     3901 aacataacac gatgttaaac aaaacaaaat attgttcaca ttcactgtga ccaaccgcga
     3961 tcatgtctcc tctcggtggc gtgtgttcgt gcatcttcct cgcgccgcca ttcgtcccag
     4021 gtacgattaa aacccagcga gcggaacaga tgatccggct gtttttctgg tgtgttgacg
     4081 gcagcaaaaa gcggtgcctc acgggttttc agatgaaact ccataagttc cggcgcaggt
     4141 gatatgtggc tgtctgcatc gtgatttacg gcaacaccat accctgcata ccgtaacccc
     4201 ttatcggtca gtgcactgat gaccagctgg ctgttatcgg ctgagggaac ggcaaacgcg
     4261 ggatacagcg taccattaag ccggacaaca ccgcccagag agtcatccac gcccatgacc
     4321 agcaaaagct gaagctggcg ggtttcccgg tcgacagaac tcattaccgg catcagcgtc
     4381 ggtagcagac gtccctgcgt aaaatccacc ttccttgctg ccagtgatgg catggctggc
     4441 tggcggtctt ctccaagaca gaaagactcc ggccatgtct tcggctggcg gagattgcgg
     4501 gtatattgcg gccatgtctg gcaaacctgc gtcagaagtt gctcaaaggt cgcagaggaa
     4561 taaacacgtt gcagtggtgg atgttgaccg atttcgcttc ggtcacagat ataaccactc
     4621 ctgtcacgca ttaaatcaac acgctcaaag attctggtgc ggtatttctc cagctcgttc
     4681 ccccagagcg tgtattctcc gttccggttg tgaagcagaa ggtaacaggt catttccccg
     4741 ttaccaggca tgggggcctc gccatacccc gtaacgactg cgctcagtac ccctgaatcc
     4801 ataacggcat tgtcattttt cctgagctgt tccgctcgtc gctccgcgcg actacgttct
     4861 gtatcctcct gctccttccg gcgcaattcc tcttcacgtt taagcaactg gactctgaga
     4921 gctgcaaggc cggatgcccc ccactgctgc cactgttttt caaatgccgt ctgactcgcg
     4981 ggaatgtcct gtttctccac cttccggtaa tgctgtcgca atttttccat ttgctggctc
     5041 agcgactgga tttttccctg ttcgctacgg tattccggtg ccgcccattg catcagggga
     5101 atacgggaaa aaccggacga aaacggcgat gattttttca ggctggcagc atggctcatc
     5161 gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc gcgtcgcctg catatctgcc
     5221 atcatcgaca tggcattact ggtgagtttc catgccgcat tactgtcctt cggcaacgct
     5281 tcgatgatgt cacgtagtgt gtacgaagtt gcgttgtcag gaagcacgat attcagaagg
     5341 tcagccacac ggcgggcagg ctctgtcagc ccgctgtttc tgagttcggt acgggaaatc
     5401 tgtatttcca ggttaagtct gttaatcaga cgattctgtt cgccgcgttc tgtttcgata
     5461 ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa taccaggcgt tttctcaagc
     5521 ccctgtcgct cgtatgaacg atggtctatc tcttcctgat aaccggcgtt cgccagtgcc
     5581 ctgttcgcat ggtcagccca tgatgcacgc cacgtctcgg catgtgtccg gtcgttccag
     5641 tccctgacct ttccaccgaa tccagcaggt c
//
