LOCUS       CP088747                9563 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC0942 plasmid pB16EC0942-3, complete
            sequence.
ACCESSION   CP088747
VERSION     CP088747.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391574
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:00:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,328
            CDSs (total)                      :: 5,194
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 134
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 139 of 215
            Pseudo Genes (internal stop)      :: 38 of 215
            Pseudo Genes (multiple problems)  :: 48 of 215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC0942"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC0942-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(9559..9563,1..1162))
                     /gene="malQ"
                     /locus_tag="LQT46_24530"
                     /pseudo=""
     CDS             complement(join(9559..9563,1..1162))
                     /gene="malQ"
                     /locus_tag="LQT46_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /name="malQ"
     gene            complement(1172..3372)
                     /locus_tag="LQT46_24535"
                     /pseudo=""
     CDS             complement(1172..3372)
                     /locus_tag="LQT46_24535"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /name="glycogen/starch/alpha-glucan phosphorylase"
     gene            4214..4993
                     /locus_tag="LQT46_24540"
     CDS             4214..4993
                     /locus_tag="LQT46_24540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI93437.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYGK
                     TTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQYAS
                     LTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSRNLP
                     HWALPICVFVTSCWKLAVSNWHLPIRKRNSFLNCRLSSPIEAAESSRICDDVSGWATAL
                     QLIALSARQNTHSAHKSARRLAGINASHLRIIWSMRF"
                     /name="hypothetical protein"
     gene            5161..5502
                     /locus_tag="LQT46_24545"
     CDS             5161..5502
                     /locus_tag="LQT46_24545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI93432.1"
                     /translation="MGFCYHPLFGNFLRQRCQWELAAELPEIHRAAAESWMAQGFPSEA
                     IHHALAAGDALMLRDILLNHAWSLFNHSELSLLEESLKALPWDSLLENPQLVLLQAWLM
                     QSQHRLRRS"
                     /name="hypothetical protein"
     gene            5653..5931
                     /locus_tag="LQT46_24550"
     CDS             5653..5931
                     /locus_tag="LQT46_24550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI93433.1"
                     /translation="MPPGWFYSRIVATSVLGEVLHCKGRIDPLTGVNAANRQDGTPARM
                     SGTTLCWSLIQQSEILFAQGFLQTAWENAGKSVPADQRAASGTAANA"
                     /name="hypothetical protein"
     gene            5928..6068
                     /locus_tag="LQT46_24555"
     CDS             5928..6068
                     /locus_tag="LQT46_24555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI93434.1"
                     /translation="MSFWCAFVRQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCWQ
                     C"
                     /name="hypothetical protein"
     gene            6062..6689
                     /locus_tag="LQT46_24560"
                     /pseudo=""
     CDS             6062..6689
                     /locus_tag="LQT46_24560"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            6701..6928
                     /locus_tag="LQT46_24565"
     CDS             6701..6928
                     /locus_tag="LQT46_24565"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="UGI93435.1"
                     /translation="MPELIRTSPLTQREWQVLGLIYSGYSNEQIAGELEVAATTIKTHI
                     RNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(6971..7393)
                     /locus_tag="LQT46_24570"
                     /pseudo=""
     CDS             complement(6971..7393)
                     /locus_tag="LQT46_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="catalyzes the conversion of terminal3'-phosphate of
                     RNA to the 2',3'-cyclicphosphodiester; frameshifted;
                     incomplete; partial on complete genome; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /name="RNA 3'-terminal phosphate cyclase"
     gene            7377..7760
                     /locus_tag="LQT46_24575"
                     /pseudo=""
     CDS             7377..7760
                     /locus_tag="LQT46_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="sigma 54-dependent; regulates genes involved in
                     forming a 2',3'-cyclic phosphodiester on RNA; incomplete;
                     partial on complete genome; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /name="sigma 54-dependent transcriptional regulator"
     gene            complement(7742..8500)
                     /locus_tag="LQT46_24580"
                     /pseudo=""
     CDS             complement(7742..8500)
                     /locus_tag="LQT46_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /name="DeoR/GlpR family transcriptional regulator"
     gene            complement(8517..9050)
                     /gene="glpG"
                     /locus_tag="LQT46_24585"
     CDS             complement(8517..9050)
                     /gene="glpG"
                     /locus_tag="LQT46_24585"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF008155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="UGI93438.1"
                     /translation="MMIACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALM
                     HFSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGV
                     VYALMGYVWLRGERDPQSGIYLQRWLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGL
                     AMAFVDSLNARKRK"
                     /name="glpG"
     gene            complement(9059..9340)
                     /locus_tag="LQT46_24590"
     CDS             complement(9059..9340)
                     /locus_tag="LQT46_24590"
                     /inference="COORDINATES: protein motif:HMM:NF023544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI93436.1"
                     /translation="MITSFANPEWRRRLLITWRHRVLSSRFQQHNQSDVWLADESPGRT
                     RAAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPSLPPCVNAQVR"
                     /name="hypothetical protein"
     gene            complement(9391..9562)
                     /gene="glpE"
                     /locus_tag="LQT46_24595"
                     /pseudo=""
     CDS             complement(9391..9562)
                     /gene="glpE"
                     /locus_tag="LQT46_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /name="glpE"
ORIGIN
        1 aggtttccgc cccaccttct cgctacgcca accgccagat caccatacaa gccaatcgca
       61 tttcatagcc ctggcttatc tcccagcagg cggcaaacct ggctgtaaag ccagccactg
      121 caaccagaga taaaaatcga cgtcatcacg atgttctgcg cagaactggc gcacttctgg
      181 cgaatccacg ttctgataca tctctggcca tgctggccag ccccagcgca tttcgtcctc
      241 tttcacttgc tgggcatgta gcgcatcaaa ggctgcctgc cagaacaggc tgtcgccctg
      301 ctctgcaaca aactggcgaa acgcggtcat ctgctcatca tcacgttgcg cgaaaccttt
      361 ccacgccatt cgtaatgctg tcatttttag ggcggtaacc gtggagtaat caaacccagt
      421 cggcatcccg cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac caggcctgag
      481 cctcttcgct aagatgggaa atcttcaact gcgttaacgt cgatataaat cacattcagc
      541 caacggcgag gaagacgggc tgtacgggct ggcactttcc ggatttgcca gatagagcgc
      601 atgaatcggg ttcagaccaa taaacgaccc agccacgttt tgccacatcc accagcatcg
      661 ctttgagatc gccaaaatcc ccatacccca gttttttttc cgatcgcagc gtataaagct
      721 gaacgcaggc acccacagct tttgttttat tcagcaacgc ctgcggttcg taacagcgtt
      781 tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtaagc gtcagcgtgt
      841 gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtc acatggcctt
      901 tgtactgctt tccttcttcg tggtcagcag ccagctatat tcgccgctgc cctccaccac
      961 catcggcatt tttttgccgc tggtataact atgacattcg ggactggcgt taccgccact
     1021 ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc
     1081 gactgcggtt taccgtgggc attgatgtaa ttggggctaa tcccgccgcc agcgcggcat
     1141 tatccagacg tttgctttcc atcgaacttc cttagcgttt tgcctgccag atacgagcct
     1201 gataatcgcg aatagagcga tccgagctaa acataccgca gcgggcggta ttgaggatcg
     1261 ccgcgcgagt ccaggcctcc tggtcgcggt acagcacatc cacctgcttt tgtgcctcca
     1321 cataagcggc gaaatctgcc atcaccagat acggatcgcc gccctgtttg ccgatactgt
     1381 gcagcatctg gtcgaaggca tgcttatcgc cgtcgctgta tttaccgctt tccagctctt
     1441 tcaatactgc gtccagcacc ttatctttct tccgccattt caccgggtcg tagcctttgg
     1501 ccagaattgc cttcacttgt tccacggtat gaccaaaata aagatatttt cttcgccgac
     1561 tttctcagcg atttcaacgt tcgcaccatc cagcgtaccg acagtaagcg caccattgag
     1621 cgccagtttc atattgccgg tacggaagct tcttacctgc agtcgaaatt tgttcggaga
     1681 tatccgccgc cgggatcagt ttttccgccg ccgaaacaca ataatccggc aagaacacca
     1741 ccttcaactt gtcgccaacc agcgtgatcg ttgttgatca cgtcagccac tttgttgatc
     1801 gcaaagataa tattttcgcc aggtagtagc ccggtgctgc tttcgcgccg aagaggaaga
     1861 cgcgcggtac gcggtcagac tgcgggtttt cacgaatttc tttgtacaac gccagaatat
     1921 gcagcagatt cagatgctgg cgtttgtact catgcagacg tttgaatctg aatatcgaaa
     1981 atcgcctgtg gattgatctc aataccggta cgaactttca caaactctgc cagacggact
     2041 ttattcgcct gcttgattct cgcgatattg ctgacggaat ttcgcatcat cagcgaattt
     2101 ttccagattg atcattgaat cgagatcgtt agcccactct tttttcagtg atttatccaa
     2161 cagagccgcc agtgcgggtt gcactgtttg acccagcgac gtggggtaat accgttggtg
     2221 acattatgga atttgttcgg ccatagctgg tgatattccg ggaacagatt ctttcaccac
     2281 cagatccgag tgcagcgccg caacaccgtt caccgcgaaa ccgccaacca cacacaggtt
     2341 cgccatatgc acttgtttgt cgtgcaccac cgccagtttg gcccacactt tttcatcgcc
     2401 cggccaggtt ttctccacta gcgtttaaag cgagtattaa tttcgttaat aatctgcatg
     2461 tggcgcgcag tagcctttca ccagtttcac atcccagcgt tccagcgctt ctggcatcag
     2521 gggtatggtt ggtgtaaagc gaaagttttg ctggtaatgc ccaggcgtca tccagctcat
     2581 ctggtgctca tcgatcagca cgcgcagcag ttctggaatc gcgatttgtt ggatgggtat
     2641 cgttcagctg aataacttcg taatccgcca gttcgtgcaa tttacgcccc gccagatgat
     2701 ggcgacgcaa aatatccgct accgagcagg ccacactgga agtattgctg catcaggccg
     2761 cagcttttta ccggcagtat ggttgtcgtt ttggatagag aacttttggt cagtttttct
     2821 gcgttgatgc cctgctgttc ggcacgcagg aaatcaccat cgttaaattt agtcagatca
     2881 aacggatgcg catgcgtcgc ctgccacaga cgcagcggct gcgcccacgc cattacgata
     2941 gccgacaacg gggagagtcc cacgctgacc ggtaatggta aattcccggc tccctagcgt
     3001 ccgtctttcg tgcactttac cgccaatgcc tacctgcaca tccagtgctt cgttgtggcg
     3061 gaaaccacgg gtagttaccg cgatgccagt catccggcgc ttcaacctgt ttgccatcga
     3121 caaaagactg gcggaacaac catattgata gttcagaccg taacccgtcg cagactgacc
     3181 gacagttgcc attgagtcga gaaagcacgc cgccagacgt cccagaccac cgttccccag
     3241 cgccgggtcg atctcttctt ccaacaggtc agtcaggttg atgtcataag ccttcaccga
     3301 atcctgtaca tcctgatacc agccgagatt caacagattg ttgcccgtca ggcgccaatc
     3361 aaaaacttcc attgagatgt agttaacatg ccgctgattc gccaccgttt ggcaaatggc
     3421 tgagcacgca gcacttttcg gccagtgctt cgctcactgc cagccaccac tgacgagggg
     3481 tcatgtcagc cgcagaattt aaagccataa cgctgccact gacgtgaaag cgcttcctga
     3541 aattgcttat cgttaaaaat aggttgtgac ataggagttc cacttttctt agattttcaa
     3601 cacaacgtta tcgctacagt ttgccaggct cgatgttgac cttccctcat cctgcggggg
     3661 attaggcagg gaggagtttg cggggatgag caaggaaatg tgatctcgac cacttaaagc
     3721 tagtgccaat ccacaggagt tagcaagcaa atcaatgcca taaccgcgca ggaaaaatct
     3781 gtatctaaag tgcaaaaaat ggccgttgcg tattttcaaa aaagcggaag gtaactctat
     3841 aaattaagta aaggagtgaa acagtttcac aagtaaaata tcacgtgtgc tccatctcat
     3901 tcttaataga tttattaaga ttcatctttt tagatggcac tttcatcaga aatgaagaag
     3961 aaacccttgc ttaaatgaga actgatgaac ataaggaaaa taccgtacaa cccagtattc
     4021 acgctggatc agcgtcgttt taggtgagtt gttaataaac atttggaatt gtgacacagt
     4081 gcaaattcag acacataaaa aaaaacgtca tcgcttgcat tagaaaggtt ttctggccga
     4141 ccttataacc attaattacg aagcggcaaa aaaaaataat atttcctcat tttccacagt
     4201 gaagtgatta actatgctga ttccgtcaaa attaagtcgc ccggttcgac tcgaccatac
     4261 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     4321 gatcacaagt cctgcgggct acggaaagac cacgctcatt tcccagtggg cggcaggcaa
     4381 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     4441 ctatctcatt gccgccgtgc aacaggcaac caacggtcac tgcgcgatat gtgagacgat
     4501 ggcgcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
     4561 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
     4621 tgtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcacccttgt
     4681 ggtgttgtca cgcaaccttc cgcactgggc attgccaatc tgcgtgttcg tgaccagctg
     4741 ctggaaattg gcagtcagca actggcattt acccatcagg aagcgaaaca gttttttgaa
     4801 ttgccgtctg tcatcgccga ttgaagctgc agaaagcagt cggatttgtg atgatgtttc
     4861 cggttgggcg acggcactgc agctaatcgc cctctccgcc cggcagaata ctcactcagc
     4921 ccataagtcg gcacgccgtc tggcgggaat caatgccagc catcttcgga ttatctggtc
     4981 gatgaggttt tagataacgt cgatctcgca acacgccatt tctgttgaaa agcgccattt
     5041 tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccag gcgaagaaaa acgggcaaat
     5101 gcgcctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
     5161 gtgggttttt gctatcaccc actgtttggt aacttcctgc gccagcgctg ccagtgggaa
     5221 ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga
     5281 tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat
     5341 attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag
     5401 tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttggt gttattgcag
     5461 gcgtggctga tgcaaagcca gcatcggcta cggcgaagtt aacaccctgc tggcccgcgc
     5521 tgaacatgaa atcaaggaca tcagagaagg caccatgcac gcagaattta acgctctgcg
     5581 cgccaggtgg cggattaaac gatggtaatc ggatgaagcg gaacggctgg caaaactggc
     5641 acttggaaga gcgtgccgcc gggctggttc tatagccgca ttgtggcaac ctcggtgctg
     5701 ggtgaagtgt tgcactgcaa agggcgaatt gacccgctca ctggcgttaa tgcagcaaac
     5761 cgacaagatg gcacgccagc acgaatgtct ggcactacgc tttgttggag tttaatccag
     5821 caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaaa cgcaggaaaa
     5881 agcgttccag ctgatcaacg agcagcatct ggaacagctg ccaatgcatg agtttctggt
     5941 gcgcattcgt gcgccaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg
     6001 cgcgcagcgg gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgctggc
     6061 aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta gccagctgaa
     6121 ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct ctaacgccaa
     6181 caaagtccgg gtgatttact ggcaaatgac cagcggataa agccgccgct gccaactggt
     6241 tgcgtcatac ggctaaacca gagtttgcga acaaccactt cctgcaaggt caatggcgca
     6301 acattgcccg tgcacaaatc ttgctgggcg agtttgaatc tgcggaaatt gtcctcgaag
     6361 aactcaatga aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc
     6421 tgcttaatca actgtactgg caggccggac gtaaaagtga cgctcagcgt gtgttgctgg
     6481 acgcattaaa actggcgaat cgtaccggat ttatcagtca ttttgtcatc gaaggcgaag
     6541 cgatggcgca acaactgcgc cagcttattc aactcaatac gctgccggag ctggaacagc
     6601 atcgcgcaca gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt
     6661 tcgatgagaa tttcgtgaac gtctgctaaa tcatccggaa gtgccagagc tgatccgcac
     6721 cagcccgttg acccaacgtg aatggcaggt cctggggctg atttactccg gttacagcaa
     6781 tgagcaaatt gcaggcgagc tggaagtggc ggcaaccacc atcaaaacgc atatccgtaa
     6841 tctgtaccaa aaactcggtg tcgcccaccg tcaggccgcg gtacaacacg cgcagaaact
     6901 gctgaagatg atgggatatg gggtgtgagt ttagccggat aatgcgtcgg atccggctta
     6961 catctttaca tcattcaatg ctaacccgcg ttacgccatc tgtttctatc aaaccaaacc
     7021 gcaccggcaa gaaacgctcc aaccacggcg atattggtca gcagatgaca tgagggatgg
     7081 gcgaccgtaa attctcccgc gcccgccagc gccatcggta gcaccagttg gtcggcgaga
     7141 tattccccca ccgccgccgg gcttgccagg tagcgttttc acctctttca ccaactgtgc
     7201 cgcgaccacc tcggcactga cgcgcttttc accgacgaca aaaaagcgtt cggtgatatt
     7261 ttcactttcg acttcaagcg agacggtatt acccggcccc tggtcgcgcg gcagggttat
     7321 gaatattctg ttcatgcaag gaaaaactac ccgccagtgt agcgatttca cgctcagcaa
     7381 catggcgcgg taactttcgc gaactttctg ccagcgtcac gcggatggcc acctttgcca
     7441 ctagcggacg catcacactg gatacagtgg aagatgagat caaccgtctg cgctataact
     7501 ggcaggagag tcgcccctcc acgcttacgg cgttgctggg cgcagaggca gaaaacatcg
     7561 atctcttcga ccgtatgcaa ctggaacacg ttatcgctat ctgccgccag gcaaagtcgc
     7621 tttctgctgc cggacgtgaa ctgtttgaca tttcgcgcca gggtaaagcc agcgtcaatg
     7681 acgcggatcg gctacgcaaa tacctggcgc gttttggtct gacatgggaa gcaatgcagg
     7741 atcagcacag ctccagttga atatgatggt ccttcagcac ctgcatcacg ctcgcaggcg
     7801 gcatcgtgtc ggtgtagaca gcatcgacca tgctgatgct acccatattg accattgcgt
     7861 tacggccaaa ttttgagtgg tcgacaacca gcataacgtg gcgcgagttc tcaatgatgg
     7921 cgcgtttggt gcgaacttcg tggtaatcga actccagtag cgagccgtcg ctatcgatgc
     7981 cgcttatccc cagaatgccg aaatcaaggc ggaactggga gataaaatcg agcgttgctt
     8041 cgccaatgat cccgcatcgc ggctgcgtaa ttcgccaccg gcaagaatga tacggaaatc
     8101 ctcttttacc atcagcgtgt tagcgacgtt gagattgttg gtcacaatgc gtaaattgct
     8161 gtgattaagg cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg ataaacagcg
     8221 tcgagccatt gggaatttgc tccgccactt tgcgggcgat gcgctctttt tcttcggtct
     8281 gggtggcctt gcgatcgtgc cacggcgtgt taaccgaact ggaaggcagc gccgcaccgc
     8341 catgatggcg caggatcagg ttttgctctg ccagctcgtt gagatcgcgg cgaatagtct
     8401 gcgggctaac ggagaaatgc tctaccagct cttcggtgct gacataaccc tgctgtttaa
     8461 ccagttcgat aataccgttg tgacgttgtg tttgtttcat tgataaatcc ctggaattat
     8521 tttcgttttc gcgcattgag cgaatcaaca aaagccatcg ctaaacccac ggctaacccg
     8581 gcgatatgtg ctccgttcgc catcgacatc ccaaacaaat caaaccatcc ggcgacaatc
     8641 cagatcaacg caaagataat taaccaacgt tgcaggtaaa tgccactttg cggatcgcgt
     8701 tcgccacgta gccagacgta gcccatcagc gcatacacca cgccagaaag cccgccaaac
     8761 cacggcccgc tgaatttttg ctgcacatag ccgcttaaca gggcgctaat gagagtaatg
     8821 acaattagct taccgctacc gaggcgtttt tccaccgcac cgccgagata ccaccaccag
     8881 agcaggttaa agaggatatg catcagcgag aagtgcatta acgcgtgggt gaagtaacgc
     8941 cagaactcaa atttcagcgt cggatcgaat ggccaggcca gccataacat cacttcctga
     9001 tcgccgagaa tttgcatggc gataaacacc accacgcagg cgatcatcat cacccaggtt
     9061 accggacctg cgcgttcacg caaggcggca aagaaggata acggcgataa tgcaggccac
     9121 tgtcggtatg accggactgc caactggcgg cgagataacg tggatctgcc gggttttcga
     9181 gaaaacgagc cagctccgcc gcacgcgttc ggcctgggga ctcatccgcc agccagacat
     9241 cgctttggtt atgttgttga aatcgtgagg ataacaccct gtgtcgccat gtaatcaaca
     9301 aacgcctgcg ccactcgggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt
     9361 attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa
     9421 ctggcgctgc caggcttcaa aaccgccatc aatgctgtag accaaatcgt agccctgttg
     9481 cagcagatac tgcgccgcgc cttgctgcta ttgccgtgat aacacatcac catcaccgtg
     9541 tgtcaaagtc gttatcacgc agt
//
