LOCUS       CP088720                4023 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC1174 plasmid pB16EC1174-4, complete
            sequence.
ACCESSION   CP088720
VERSION     CP088720.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391582
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 243x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:42:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,687
            CDSs (total)                      :: 4,576
            Genes (coding)                    :: 4,360
            CDSs (with protein)               :: 4,360
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 86 of 216
            Pseudo Genes (incomplete)         :: 160 of 216
            Pseudo Genes (internal stop)      :: 36 of 216
            Pseudo Genes (multiple problems)  :: 55 of 216
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC1174"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC1174-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(2534..4023,1..621))
                     /locus_tag="LQR06_23360"
                     /pseudo=""
     CDS             complement(join(2534..4023,1..621))
                     /locus_tag="LQR06_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            800..1054
                     /locus_tag="LQR06_23365"
     CDS             800..1054
                     /locus_tag="LQR06_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UGI44753.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            1586..2278
                     /locus_tag="LQR06_23370"
     CDS             1586..2278
                     /locus_tag="LQR06_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGI44754.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(264..622)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          623..799
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1587..2278
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..708)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.305"
                     /coverage="98.0"
                     /evalue="2.04e-152"
     Typing          complement(2538..3823)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.767"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ggcagacttt ccgaggtgaa taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg
       61 atggtctatc tcttcctgat aaccggcgtt cgccagtgct ctgtttgcat ggtcagccca
      121 tgatgcacgc cacgtctcgg catgtgtccg gtcgttccag tccctgacct ttccgccgaa
      181 cccagcaggt ccgacagccc tcgtggtcag catgatgtgc gcatggggat ttgttttatc
      241 catgtgatgg aacgccacat cagcaatcat gccctgactg acaaagtttt ctctgacaaa
      301 ggcgagaacg gtttcccgtg cggcatcctg cggcagttca cgggggatag cgagttcaat
      361 ttcccgcgca agttgtgagt tcttccggca ttcggccttc tcaacggcat tccagagttc
      421 ggcacggttc gtacaccagg caggggctgt cgcgggggtc agaatcacag cttcagccac
      481 accgcttttg cgggtgtagt cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc
      541 ggaacgatag gcagcggaag ctacggcgct gtagccgctg cttctggaga taattttcat
      601 gctgagatga taaatcgcca tttctgccgt aatgcctttt tgtagttttc tttcagcccc
      661 gcagggcgca cacaatcgaa acggagtgga gattgtataa gtgcgcactt tgtgatttgt
      721 cgcgcattat gccagctcag tttttaaact accagtgacg cgctaaaatg cgatttttca
      781 tcgcgcacgg agttacatca tgagcagaac gctcgaacag aagattgctg atgctgaggc
      841 gagattacag cgcctgaagg cgaagagccg gagcctggac acagcgcaga aggttgttgt
      901 gggtgcagca ttgctggcaa aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt
      961 actccagttc ctgaaggcag aggtgacacg acaggctgat gtgacgcgca tactgccgct
     1021 gattaacgag ctggaggcgc tgccaggaca gtgattgtat cgtccgctcg ccgcagcccc
     1081 agcgaccgag cgttagcgag cgagtgggcg aggaagcgga agtgaatccg tcgccaggtt
     1141 gagcacatac gcacggtttt tcggtaggtg atatgcgctg aacccgcatg aatactggat
     1201 ttccacaggg ttggaagggg agtattcgga acttgcaatt ctgctttctt gcaacacatt
     1261 gtttttattg gttttttgtt ttcagcgccc caagttatcc acaggctcgc cgttaagttt
     1321 ttagatcttg tttttaagat cttgttttta atacttgttt tagggtcaaa attcattaat
     1381 aaaatcaata tgttatagag gtatatgtgg aggaatttaa ggtaaaggtg gaggaattta
     1441 aggtgtttag gtggaggaat ttaaggtaaa ggtggaggaa tttaaggtgt tattcacaga
     1501 aatgtgagga tataaaaaaa aatattccac atttcttatt tgtggggcac attaaagcat
     1561 caccacattt gaacgggatc tttgtatgtc ggacttgatt gcctacaagt cgaatgcttt
     1621 ggttgaggcc agctacaagt tgaccctaca ggaacaacgt tttctgttgc tgtgtatcag
     1681 tcgattgaaa tctggtgctg atgctgaatt gcaaaagaca atgaccatta ccgctgcgga
     1741 atattttgat tcattccctg acatggggcg gaaaaatgcc gaggttcagt tacaggaagc
     1801 gatcgatcgt ttatgggaca ggtcgatcat tctcaagaat gacgagaagc gtgaggagtt
     1861 ccgctggatc cagtatcggg cacagtacgc taaaggtgag gccaaggctc gaataacctt
     1921 ttctgacgcg gtaatgcctt atctgacgca actgaagggg caattcacgc gcgttgttat
     1981 aaaaaacata tcaggtttat caagtagtta cagtataaga atatacgagt tcctccagca
     2041 attccgcagt acgggagaga gaattattgc tcttaatgac tttcgttcaa tgttagggat
     2101 tgaaaataaa tacaaacaat tcagagactt aaataaaata ctaataaaac cctgtgttga
     2161 cgaactgaac aaaaaaagcg acctggctgt aacggttgag acgatcaaaa aggggcgcac
     2221 ggttgtagcc ctgcattttc gcttcaagga agacaaacag atcaagatga cgatctaaaa
     2281 tcgattttaa ggcacctaaa acgctccctg ctgcgttttt tttgtgttag tagtgcgaag
     2341 gtactattga agtgaaaaat tgcgtcaaaa gcgctctaat gcagcgaaaa aagaaccccc
     2401 aatttttggg ggcggaaaaa caaaactatg cttgcattat gtgtgtgctt cttgcattca
     2461 tccctgtcga tgaacataga agaacatcga cgaacataac acgatgttaa acaaaacaaa
     2521 atattgttca cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc
     2581 ttgcatcttc ctcgcgccgc cattcatccc aggtacgatt gaaacccaga gagcggaaca
     2641 gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt
     2701 tcagatgaaa ctccataagt tccggcgcag gtgatatgtg gctgtctgca tcgtgattta
     2761 cggcaacacc ataccctgca taccgtaacc ccttatcggt cagtgcactg atgaccagct
     2821 ggctgttatc ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggacaa
     2881 caccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc
     2941 ggtcgacaga actcattacc ggcatcagcg tcggtagcag acgtccctgc gtaaaatcca
     3001 ccttccttgc tgccagtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact
     3061 ccggccatgt cttcggctgg cggagattgc gggtatattg cggccatgtc tggcaaacct
     3121 gcgtcagaag ttgctcaaag gtcgcagagg aataaacacg ttgcagtggt ggatgttgac
     3181 cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca acacgctcaa
     3241 agattctggt gcggtatttc tccagctcgt tcccccagag cgtgtattct ccgttccggt
     3301 tgtgaagcag aaggtaacag gtcatttccc cgttaccagg catgggggcc tcgccatacc
     3361 ccgtaacgac tgcgctcagt acccctgaat ccataacggc attgtcattt ttcctgagct
     3421 gttccgctcg tcgctccgcg cgactacgtt ctgtatcctc ctgctccttc cggcgcaatt
     3481 cctcttcacg tttaagcaac tggactctga gagctgcaag gccggatgcc ccccactgct
     3541 gccactgttt ttcaaatgcc gtctgactcg cgggaatgtc ctgtttctcc accttccggt
     3601 aatgctgtcg caatttttcc atttgctggc tcagcgactg gatttttccc tgttcgctac
     3661 ggtattccgg tgccgcccat tgcatcaggg gaatacggga aaaaccggac gaaaacggcg
     3721 atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg ttcagctctt
     3781 cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga cattcgatga tgtcacgtag
     3841 tgtgtacgaa gttgcgttgt caggaagcac gatattcaga aggtcagcca cacggcgggc
     3901 aggctctgtc agcccgctgt tcctgagttc agtgcgggaa atctgtattt ccaggttaag
     3961 cctgttaatc agacgattct gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc
     4021 aca
//
