LOCUS       CP088717                2160 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B16EC1174 plasmid pB16EC1174-2, complete
            sequence.
ACCESSION   CP088717
VERSION     CP088717.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391582
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 243x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:42:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,687
            CDSs (total)                      :: 4,576
            Genes (coding)                    :: 4,360
            CDSs (with protein)               :: 4,360
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 86 of 216
            Pseudo Genes (incomplete)         :: 160 of 216
            Pseudo Genes (internal stop)      :: 36 of 216
            Pseudo Genes (multiple problems)  :: 55 of 216
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B16EC1174"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB16EC1174-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            324..1235
                     /locus_tag="LQR06_00815"
                     /pseudo=""
     CDS             324..1235
                     /locus_tag="LQR06_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1439..1837)
                     /locus_tag="LQR06_00820"
     CDS             complement(1439..1837)
                     /locus_tag="LQR06_00820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI40536.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          325..1096
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.268"
                     /coverage="83.0"
                     /evalue="0.0"
ORIGIN
        1 aaaccgatcg cacatcgctt tcgtgcatag ttatgcaacg ccctgaaaac gattctgacg
       61 cgttttttct ggtttggcct ggtgttttct tgtctttttg cgttttttgc gccagaaagc
      121 gactgaggcg ttttaagggg ggcgtacaac gggagttatg gtaaatggat cgggtttttc
      181 gggaaggttc gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc
      241 ctgaaatcat gtccagatcc ggcaagattc aacatgaaat cagagggcgt gtaggtcgca
      301 taacccggag agaatcgtaa cacatgagcg ccgcgcttca atacttcgac gaaaatttac
      361 ccatcgtccc ctatcacacg gatatctcgc ttttggtctt cgcatctccg gcaaagggcg
      421 tgcgcttctt gcgcggtaca tagcagaacc agcctcatgc gcagttctgg ctggtttttg
      481 acgttgaccg cgagggggct gcgattgact ggagcgaccg gaacgctccc gcgccgaaca
      541 tcaccgttaa aaatcctgtg aacggacacg ctcacctgct ctacgcactc aacatcgccg
      601 tgagaaccgc gcctgatgcc tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt
      661 cgctgtgtga gaaactttgt gcggatgtga actacagcgg cctgatttgc aaaaatccgt
      721 tccacctgga atggctggtg atggagtggc gcgaggaagc ctataccctc gatgaactgg
      781 ctgattatct cgatttgagc gcctcagagc gtcgtagcat cgataaacat tacgggatgg
      841 ggcgaaactg ccacctgttc gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg
      901 gctggccagc gttctcacag tggcttgaag cggtgatcca gcgtgtcgaa atgtacaatg
      961 catcgcttcc cgttccgctt tcactggctg aatgtcgggc tattggcaag agcattgcga
     1021 aatacacgca caggaacttc acgccggaaa ctttcgcaca gtatgtggct gatacgcaca
     1081 cgccagaaat tcaggctgca cgcggtcgca agggcggttc taagtctaag cgcagcacag
     1141 tagctacatc tgcacgcacg ctgaaaccgt gggaggcatt agggatcagt cgcgcctggt
     1201 attaccaact gaaaaaacga ggtctcgtag agtagaccaa ataagcctat atcagataac
     1261 agcgcctttc tggcgtcttt ttgagcagca ggttttttgc cgtttttccc cgttcagccc
     1321 tgcaatggcg cggctttccg cgtgattgtg gtggtagtgc tcgccgcagt ctcatgaccg
     1381 agcgtagcga gcgaatgagc gaggaagcac cagggaatcg gaacacggat ggaggcactt
     1441 acgcgtctgg gtttggggat gtctccagat cgtacttaag ttccttaagt gcttgtaata
     1501 tcctgtcttt tttatagcat agctcgcctg tttttgtgtc taccagtcct gcttcgatca
     1561 gtagcccagc cgcgagaaag agccggtgat tgcgttcccg tcgttgtttt tttccctcga
     1621 ttcgtttttg gaccgcctgt tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg
     1681 ctgttgccag tacgtggaga agtttgcgtt cctgttctgt gagtccggct tcgttgtcga
     1741 tcaggtcaat aatctgtctc tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga
     1801 tccgtttctt cagccattcg atgttgctga tgcgcatgtt cgtgatgctc cctggtgttt
     1861 ttttgcggtg tgttgtggtc gtgcagtata tcgcttgcgg tcggcgggtg cagggggtat
     1921 cttccgtgct aattcgtaaa attagaagga agcgcactta tatgtttctc tccttcgtcg
     1981 agaaacagtg cgctcctgca gagggctgaa accgtggcaa cctatccctc agcgttaaat
     2041 ttggtggcaa ggggcaggct gcaaaaccac gccgattaca tcgagcgcaa agagaaatac
     2101 cgcgaccgtc aggatttgga atattccgca cacggaaaca tgccggaatg ggccagggat
//
