LOCUS       CP088709                1549 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain B17EC0157 plasmid pB17EC0157-2, complete
            sequence.
ACCESSION   CP088709
VERSION     CP088709.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391584
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 264x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:18:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,079
            CDSs (total)                      :: 4,966
            Genes (coding)                    :: 4,738
            CDSs (with protein)               :: 4,738
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 96 of 228
            Pseudo Genes (incomplete)         :: 166 of 228
            Pseudo Genes (internal stop)      :: 37 of 228
            Pseudo Genes (multiple problems)  :: 61 of 228
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B17EC0157"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB17EC0157-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.68 N 126.77 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(1439..1549,1..15))
                     /locus_tag="LQQ96_00005"
     CDS             complement(join(1439..1549,1..15))
                     /locus_tag="LQQ96_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UGI30753.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="replication protein"
     gene            518..994
                     /locus_tag="LQQ96_00010"
     CDS             518..994
                     /locus_tag="LQQ96_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UGI30754.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          37..135
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(331..439)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(1226..1486)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 aaccggaagc cacattgagc acttacgcac tgatgcgggg tgtcggggtg aagccctgac
       61 caagtggtaa tcgtatcggc gtgcatgcgc ggttatacga ttacacatcc tgtcccgatt
      121 tctgaggcgt tttaacggtg aacggacggc aaaaacacga tggagctgtt gaacgtgaaa
      181 accacgaaat ctggcaaaaa tcacggctgg cgaggcttgc ataggagtaa gccagtatac
      241 actccgctat cgctacgtga ctgggtcagg gcttcgcccc gacaccccca aagggcgttg
      301 cgttgcacgc aacacccttg cctagaatag atcttttacg ccgatttgta agtggttgtt
      361 tttttagtgt gttttttatt tacctgtcgc atccaggatg acacaacgtt gtcgcatcct
      421 ggatgcgaca acacgcaaca aggtgttgca cagatgtgtc atgatggctt agatttaaac
      481 caacgttagt gcatgggatt ttcagaggga aaaaatcatg tttattgatt cagaaaaacg
      541 actgaaacaa ctttcagatg aggcaaagaa aaacaccgag gatctcgaag aagcaaagaa
      601 aaattcaagg tttacacagg tatccccaaa aggttgggaa cgtgttcgag agctgctgaa
      661 ggatagccaa ggcatatcag cactgaagct gtactcattt ttagcggagc atatcgatcc
      721 tacgtgtggc gctgtcgttg cggatcagca attcctagct gaaaaacttg gagttagcag
      781 aagcacaatt attcggtggc tcaattactt agaatcaaaa aatgcattag ttagaatccc
      841 cgttgctggt aaggtttgtg cgtatgccct cgatccacat gaagtctgga agggatacaa
      901 cactacgaaa aaccatgcag cgtttgtcac taaaacactg gtcaacaaag acggtgatat
      961 tcagcgccga atcatggcca tgttttcaaa ttgagctagc ggcaggcgga caatcagggg
     1021 ctacgtgtta acgttctgac catgattgtc tatcctgcat tgctcttttg ccgcctcaaa
     1081 atcctttgcg tgtttttgct ccccgttctc cagaaaaaac cgagccgcca cggttccggc
     1141 agcgccttga gcgctaccgg aaaccgtctg gggagaaccc cagaccccca tcgctgcaac
     1201 accgacggtc tacgaccacc taccaaccgc atggctgcct gttccgtgca aatgggcttt
     1261 gcagggggct ggcgcggttc ctggcgacct gaaagcccct ggttaggggt atcggcgcgc
     1321 ttgacctcct gagagcctct gtaagcgttt ttcgcgcctt ccatgccctg gcgggcatct
     1381 aaaacccgtt tcgtgcgtct gagagctttt gagcgcgttt gaggggctat ctggcgactc
     1441 atccccaaaa gaacaacacc gggatcaccg acaacatgcg agaggtgatc ccgctcgccg
     1501 caagtcgatg accgagcgta gcgagcgaat cgacgaggaa gcggaagag
//
