LOCUS       CP088691                5167 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain C16EC0033 plasmid pC16EC0033-4, complete
            sequence.
ACCESSION   CP088691
VERSION     CP088691.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391591
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:24:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,037
            CDSs (total)                      :: 4,924
            Genes (coding)                    :: 4,697
            CDSs (with protein)               :: 4,697
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 93 of 227
            Pseudo Genes (incomplete)         :: 161 of 227
            Pseudo Genes (internal stop)      :: 35 of 227
            Pseudo Genes (multiple problems)  :: 53 of 227
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C16EC0033"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pC16EC0033-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.34 N 127.92 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(57..455)
                     /locus_tag="LQR02_25165"
     CDS             complement(57..455)
                     /locus_tag="LQR02_25165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI01145.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            632..1558
                     /locus_tag="LQR02_25170"
     CDS             632..1558
                     /locus_tag="LQR02_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGI01146.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1561..2094
                     /locus_tag="LQR02_25175"
     CDS             1561..2094
                     /locus_tag="LQR02_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI01147.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2160..2369)
                     /locus_tag="LQR02_25180"
     CDS             complement(2160..2369)
                     /locus_tag="LQR02_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGI01148.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            4106..5020
                     /locus_tag="LQR02_25185"
     CDS             4106..5020
                     /locus_tag="LQR02_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGI01144.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          4107..4881
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          633..1555
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtagcgagc gaatgagcga ggaagcacca gggaatcgga acacggatgg aggcacttac
       61 gcgtctgggt ttggggatgt ctccagatcg tacttaagtt ccttaagtgc ttgtaatatc
      121 ctgtcttttt tatagcatag ctcgcctgtt tttgtgtcta ccagtcctgc ttcgatcagt
      181 agcccagccg cgagaaagag ccggtgattg cgttcccgtc gttgtttttt tccctcgatt
      241 cgtttttgga ccgcctgttt tcgttcgctc tcctgtgcct gtaagtcgtt tttttccgct
      301 gttgccagta cgtggagaag tttgcgttcc tgttctgtga gtccggcttc gttgtcgatc
      361 aggtcaataa tctgtctctg tcgtgcggtc tgttctccga gcttcctgat gaatccgatc
      421 cgtttcttca gccattcgat gttgctgatg cgcatgttcg tgatgctccc tggtgttttt
      481 ttgcggtgtg ttgtggtcgt gcagtatatc gcttgcggtc ggcgggtgca gggggtatct
      541 tccgtgctaa ttcgtaaaat tagaaggaag cgcacttata tgtttctctc cttcgtcgag
      601 aaacagtgcg ctcctgcaga gggctgaaac cgtggcaacc tatcacctca gcgttaaatt
      661 tggtggcaag gggcaggctg caaaccacgc cgattacatc gagcgcaaag agaaataccg
      721 cgaccgtcag gatttggaat attccgcaca cggaaacatg ccggaatggg ccagggataa
      781 tccttcccat ttctggcagg cggctgacca gtttgaacgt gcgaacggtt cgacctatag
      841 ggaacttgaa attgcattac cccgtgaact gacaccggaa cagcgccttg aactggtgca
      901 ggattttgta cgtcaggaag ctggcgaacg gcacgcctgg agttttgcca ttcacaatcc
      961 gaaagcgagc attgacggcg gcgaacagcc tcatgcgcat atcatgatga gtcagcgtgt
     1021 gaatgatggc attgaacgta caccggaaca gtatttccgg cgctataacg ccaggtatcc
     1081 cgagcgggga ggtgcgaaaa aggattccgg aagcctgact ctcactcagc aaaaagagca
     1141 gttgcgggaa ttgcgaaaac gctgggaggt gaagcacaac gaacatatga gaaagcatgg
     1201 ctttgaaagg ggcttcattg actgccggac gctgaaagaa cagggcattg aacgacgtcc
     1261 cgaagtgcat ctgggttttg aagctgccgg acggcttgat gatgcccagc gtcatgacat
     1321 catgcaaaaa cggtctgaac cggactattc ccgccatctt gaacaggcgc gaacggtcgg
     1381 gcatgacatc agggaatggc tggacactcc ggttaagcaa catgaactgg tcagaaggct
     1441 gcaacatgat gttgcgcggg agcatggctt tgacatgaga gacagagtga gcagacagaa
     1501 ccgaactgca cctgaacggg ttaaatcacc gtcacgtgga ttttctatgg gattctgaac
     1561 atggccagca cagaagaaaa attacttacg ctcctgttat cggtggagca tctgcaacat
     1621 accgcaatgg agcaacagaa agctgttgct gattctctgg ctgtggcaag ggaaactctt
     1681 cgcggatatc gggatatttc tgacggtgtg gcaaaacagg tcagggatga agtcagaaat
     1741 gaggtgcaga agctggacgt ggcaggactg ataggtgagc gcatcagcca ggattttcag
     1801 cggctggaag agtcagtcgc aaacatgaag gcaaacgcgg atgccctgaa cagggatatt
     1861 gcgacaattc acagcaatat cgtgcaggaa tacaacaccc ttaaagggta tagctggaaa
     1921 tggctgggtg gggtgtcagt ggcttttctg gttatgttct gtgtgctcgg atggggaatg
     1981 aaaacaatac tcgacaggaa ctacaacgcc acagcggcgg tgtatcagaa agttgtgaca
     2041 ctggaagata aactggtgga gaaacctgtc acggggaaga gccggaaaaa ctgacgcgct
     2101 tcgcttgtcc aacgcctcac gacccgcgaa agaatttcgc gaaccgttca ccgttttttt
     2161 caatatgatt ctgtctgctt tggtcgataa agcagacgat agtattccag ccttcgtata
     2221 tataacggta cgtgctcatc agtaagagct ggcggtattg tgttatcagg gggaagtttt
     2281 ttcagatact cacgaaatgc ctggcttgat gccataaaat ctacagtagc atcgataata
     2341 tgtggggtgt tttcacctaa ttttgccatg atgataatcc ttatattatc acggtttgtg
     2401 aatgataggg tgtcactatt atttatgcgt tagtgaacca gaaaaaatcc cccgttggca
     2461 tagggggata tgtaaggaga aataacttcg caggtgttct tgtttgtata acgtgaaaag
     2521 actgcacgca acttaataag tctgccgtac ttactttcag ttttcaacat aaggtttata
     2581 acaatgtatg gaatggaagt gagctttaag gtttcagctc taaaaaataa aatatttatt
     2641 agttggctgg ttgttgttct tgttctgtat gtatataaac actatcgaca tattatgacc
     2701 gggaacattc cttaccccct ctgcgctcgc agaggttttt tttgtcatta attaataatg
     2761 aactgaaatg cagttccgat acccccattt gcacattatc cctgacatat ggtaccggaa
     2821 ccgcattaat tttatgttat aagagtttat ttttaatgtc cagatggttt aatagtattt
     2881 atatgtaatt agctgtatgg atagtactgg ttttattgta taacttgtcg ttttttttta
     2941 ttgtttttgt gtggtgtgct aatctttaag ttataaaaaa accccttgga atacaggggc
     3001 aaaagtaagg aaaacaaatg gatgctcagt ttgtgtgaca ctttaaacta atggaactat
     3061 agagtggagg ttgttacagt cgcttttata aaacagcaac cagttttttt caatagtctg
     3121 atgatggttg tgacagtttt gtgatattgt tcaaaatgtt tcttgttgaa tgtttgtttt
     3181 ttggtctatt ggtgtctggt tttatttttt ctgctattac atatcaagaa atgtgattgg
     3241 tgtagcaaga gtggaatgcg atgtggataa actaattggt tgtcgaatct aatttactta
     3301 gaaaactaca cttgtttttg tgtatgaatt tagtgaattt gtctgtctat gtgttttcgt
     3361 gcatttataa atccttcgtc aggatcttaa atttagtgtt gaatatgtag ggcctgtacg
     3421 gtaattatta tttagcgtag ctacttcatc gttgttcgaa cacaaccaac aacctgtaat
     3481 tgttgtttct cttggttccc cacttttgtg gggtttttta tctcttccgt gcatcgtaaa
     3541 gcgcggactc cttctggaaa ggttgtttta gggcatcact gaggcatttt ttgcgtttgt
     3601 gaatattgag catccgataa ctaaaatatt tcccatagac tctgattctt gccaccgacc
     3661 ccaggtcggt ttttttttgt cctggcgaga tgtgaagttt ggaaacagga aaccccgtca
     3721 gagcgccacg caccagggga gaaaacttgt cgttttgagc cgttcggtgt tcagaacgca
     3781 cgaaaccgat cgcacatcgc tttcgtgcat agttatgcaa cgccctgaaa acgattctga
     3841 cgcgtttttc tggtttggcc tggtgttttc ttgtcttttt gcgttttttg cgccagaaag
     3901 cgactgaggg cgttttaagg ggggcgtaca acgggagtta tggtaaatgg atcgggtttt
     3961 cgggaaggtt cgacaggatt tgccgttggg tgtagtgtaa gcgactgaaa aacaaacgcc
     4021 cctgaaatca tgtccagatc cggcaagatt caacatgaaa tacagagggc gtgtaggtcg
     4081 cataacccgg agagaatcgt aacacatgag cgccgcgctt caatacttcg acgaaaattt
     4141 accccatcgt ccctatcaca cggatgatct cgcttttggt cttcgcatct ccggcaaagg
     4201 gcgtgcgctt cttgcgcggt acatacagca gaaccagcct catgcgcagt tctggctggt
     4261 ttttgacgtt gaccgcgagg gggctgcgat tgactggagc gaccggaacg ctcccgcgcc
     4321 gaacatcacc gttaaaaatc ctgtgaacgg acacgctcac ctgctctacg cactcaacat
     4381 cgccgtgaga accgcgcctg atgcctcggt taaggcgctg aaatatgccg cagcggttga
     4441 acgttcgctg tgtgagaaac tttgtgcgga tgtgaactac agcggcctga tttgcaaaaa
     4501 tccgttccac ctggaatggc tggtgatgga gtggcgcgag gaagcctata ccctcgatga
     4561 actggctgat tatctcgatt tgagcgcctc agagcgtcgt agcatcgata aacattacgg
     4621 gatggggcga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca gggcgattcg
     4681 tcagggctgg ccagcgttct cacagtggct tgaagcggtg atccagcgtg tcgaaatgta
     4741 caatgcatcg cttcccgttc cgctttcact ggctgaatgt cgggctattg gcaagagcat
     4801 tgcgaaatac acgcacagga acttcacgcc ggaaactttc gcacagtatg tggctgatac
     4861 gcacacgcca gaaattcagg ctgcacgcgg tcgcaagggc ggttctaagt ctaagcgcag
     4921 cacagtagct acatctgcac gcacgctgaa accgtgggag gcattaggga tcagtcgcgc
     4981 ctggtattac caactgaaaa aacgaggtct cgtagagtag accaaataag cctatatcag
     5041 ataacagcgc ctttctggcg tctttttgag cagcaggttt tttgccgttt ttccccgttc
     5101 agccctgcaa tggcgcggct ttccgcgtga ttgtggtggt agtgctcgcc gcagtctcat
     5161 gaccgag
//
