LOCUS       CP088686                5167 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain C16EC0050 plasmid pC16EC0050-3, complete
            sequence.
ACCESSION   CP088686
VERSION     CP088686.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391592
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 13:24:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,733
            CDSs (with protein)               :: 4,733
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 96 of 226
            Pseudo Genes (incomplete)         :: 149 of 226
            Pseudo Genes (internal stop)      :: 36 of 226
            Pseudo Genes (multiple problems)  :: 48 of 226
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C16EC0050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pC16EC0050-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.34 N 127.92 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            22..231
                     /locus_tag="LQT94_25360"
     CDS             22..231
                     /locus_tag="LQT94_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGH96446.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(297..830)
                     /locus_tag="LQT94_25365"
     CDS             complement(297..830)
                     /locus_tag="LQT94_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGH96447.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(833..1759)
                     /locus_tag="LQT94_25370"
     CDS             complement(833..1759)
                     /locus_tag="LQT94_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGH96448.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1936..2334
                     /locus_tag="LQT94_25375"
     CDS             1936..2334
                     /locus_tag="LQT94_25375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGH96449.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2538..3452)
                     /locus_tag="LQT94_25380"
     CDS             complement(2538..3452)
                     /locus_tag="LQT94_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGH96450.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          complement(2678..3452)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(837..1759)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gataatataa ggattatcat catggcaaaa ttaggtgaaa acaccccaca tattatcgat
       61 gctactgtag attttatggc atcaagccag gcatttcgtg agtatctgaa aaaacttccc
      121 cctgataaca caataccgcc agctcttact gatgagcacg taccgttata tatacgaagg
      181 ctggaatact atcgtctgct ttatcgacca aagcagacag aatcatattg aaaaaaacgg
      241 tgaacggttc gcgaaattct ttcgcgggtc gtgaggcgtt ggacaagcga agcgcgtcag
      301 tttttccggc tcttccccgt gacaggtttc tccaccagtt tatcttccag tgtcacaact
      361 ttctgataca ccgccgctgt ggcgttgtag ttcctgtcga gtattgtttt cattccccat
      421 ccgagcacac agaacataac cagaaaagcc actgacaccc cacccagcca tttccagcta
      481 taccctttaa gggtgttgta ttcctgcacg atattgctgt gaattgtcgc aatatccctg
      541 ttcagggcat ccgcgtttgc cttcatgttt gcgactgact cttccagccg ctgaaaatcc
      601 tggctgatgc gctcacctat cagtcctgcc acgtccagct tctgcacctc atttctgact
      661 tcatccctga cctgttttgc cacaccgtca gaaatatccc gatatccgcg aagagtttcc
      721 cttgccacag ccagagaatc agcaacagct ttctgttgct ccattgcggt atgttgcaga
      781 tgctccaccg ataacaggag cgtaagtaat ttttcttctg tgctggccat gttcagaatc
      841 ccatagaaaa tccacgtgac ggtgatttaa cccgttcagg tgcagttcgg ttctgtctgc
      901 tcactctgtc tctcatgtca aagccatgct cccgcgcaac atcatgttgc agccttctga
      961 ccagttcatg ttgcttaacc ggagtgtcca gccattccct gatgtcatgc ccgaccgttc
     1021 gcgcctgttc aagatggcgg gaatagtccg gttcagaccg tttttgcatg atgtcatgac
     1081 gctgggcatc atcaagccgt ccggcagctt caaaacccag atgcacttcg ggacgtcgtt
     1141 caatgccctg ttctttcagc gtccggcagt caatgaagcc cctttcaaag ccatgctttc
     1201 tcatatgttc gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt
     1261 gctgagtgag agtcaggctt ccggaatcct ttttcgcacc tccccgctcg ggatacctgg
     1321 cgttatagcg ccggaaatac tgttccggtg tacgttcaat gccatcattc acacgctgac
     1381 tcatcatgat atgcgcatga ggctgttcgc cgccgtcaat gctcgctttc ggattgtgaa
     1441 tggcaaaact ccaggcgtgc cgttcgccag cttcctgacg tacaaaatcc tgcaccagtt
     1501 caaggcgctg ttccggtgtc agttcacggg gtaatgcaat ttcaagttcc ctataggtcg
     1561 aaccgttcgc acgttcaaac tggtcagccg cctgccagaa atgggaagga ttatccctgg
     1621 cccattccgg catgtttccg tgtgcggaat attccaaatc ctgacggtcg cggtatttct
     1681 ctttgcgctc gatgtaatcg gcgtggtttg cagcctgccc cttgccacca aatttaacgc
     1741 tgaggtgata ggttgccacg gtttcagccc tctgcaggag cgcactgttt ctcgacgaag
     1801 gagagaaaca tataagtgcg cttccttcta attttacgaa ttagcacgga agataccccc
     1861 tgcacccgcc gaccgcaagc gatatactgc acgaccacaa cacaccgcaa aaaaacacca
     1921 gggagcatca cgaacatgcg catcagcaac atcgaatggc tgaagaaacg gatcggattc
     1981 atcaggaagc tcggagaaca gaccgcacga cagagacaga ttattgacct gatcgacaac
     2041 gaagccggac tcacagaaca ggaacgcaaa cttctccacg tactggcaac agcggaaaaa
     2101 aacgacttac aggcacagga gagcgaacga aaacaggcgg tccaaaaacg aatcgaggga
     2161 aaaaaacaac gacgggaacg caatcaccgg ctctttctcg cggctgggct actgatcgaa
     2221 gcaggactgg tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacaa
     2281 gcacttaagg aacttaagta cgatctggag acatccccaa acccagacgc gtaagtgcct
     2341 ccatccgtgt tccgattccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg
     2401 agactgcggc gagcactacc accacaatca cgcggaaagc cgcgccattg cagggctgaa
     2461 cggggaaaaa cggcaaaaaa cctgctgctc aaaaagacgc cagaaaggcg ctgttatctg
     2521 atataggctt atttggtcta ctctacgaga cctcgttttt tcagttggta ataccaggcg
     2581 cgactgatcc ctaatgcctc ccacggtttc agcgtgcgtg cagatgtagc tactgtgctg
     2641 cgcttagact tagaaccgcc cttgcgaccg cgtgcagcct gaatttctgg cgtgtgcgta
     2701 tcagccacat actgtgcgaa agtttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     2761 ctcttgccaa tagcccgaca ttcagccagt gaaagcggaa cgggaagcga tgcattgtac
     2821 atttcgacac gctggatcac cgcttcaagc cactgtgaga acgctggcca gccctgacga
     2881 atcgccctgt aagcccattt gcgcgtcatt tcgaacaggt ggcagtttcg ccccatcccg
     2941 taatgtttat cgatgctacg acgctctgag gcgctcaaat cgagataatc agccagttca
     3001 tcgagggtat aggcttcctc gcgccactcc atcaccagcc attccaggtg gaacggattt
     3061 ttgcaaatca ggccgctgta gttcacatcc gcacaaagtt tctcacacag cgaacgttca
     3121 accgctgcgg catatttcag cgccttaacc gaggcatcag gcgcggttct cacggcgatg
     3181 ttgagtgcgt agagcaggtg agcgtgtccg ttcacaggat ttttaacggt gatgttcggc
     3241 gcgggagcgt tccggtcgct ccagtcaatc gcagccccct cgcggtcaac gtcaaaaacc
     3301 agccagaact gcgcatgagg ctggttctgc tgtatgtacc gcgcaagaag cgcacgccct
     3361 ttgccggaga tgcgaagacc aaaagcgaga tcatccgtgt gatagggacg atggggtaaa
     3421 ttttcgtcga agtattgaag cgcggcgctc atgtgttacg attctctccg ggttatgcga
     3481 cctacacgcc ctctgtattt catgttgaat cttgccggat ctggacatga tttcaggggc
     3541 gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgaac cttcccgaaa
     3601 acccgatcca tttaccataa ctcccgttgt acgcccccct taaaacgccc tcagtcgctt
     3661 tctggcgcaa aaaacgcaaa aagacaagaa aacaccaggc caaaccagaa aaacgcgtca
     3721 gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc ggtttcgtgc
     3781 gttctgaaca ccgaacggct caaaacgaca agttttctcc cctggtgcgt ggcgctctga
     3841 cggggtttcc tgtttccaaa cttcacatct cgccaggaca aaaaaaaacc gacctggggt
     3901 cggtggcaag aatcagagtc tatgggaaat attttagtta tcggatgctc aatattcaca
     3961 aacgcaaaaa atgcctcagt gatgccctaa aacaaccttt ccagaaggag tccgcgcttt
     4021 acgatgcacg gaagagataa aaaaccccac aaaagtgggg aaccaagaga aacaacaatt
     4081 acaggttgtt ggttgtgttc gaacaacgat gaagtagcta cgctaaataa taattaccgt
     4141 acaggcccta catattcaac actaaattta agatcctgac gaaggattta taaatgcacg
     4201 aaaacacata gacagacaaa ttcactaaat tcatacacaa aaacaagtgt agttttctaa
     4261 gtaaattaga ttcgacaacc aattagttta tccacatcgc attccactct tgctacacca
     4321 atcacatttc ttgatatgta atagcagaaa aaataaaacc agacaccaat agaccaaaaa
     4381 acaaacattc aacaagaaac attttgaaca atatcacaaa actgtcacaa ccatcatcag
     4441 actattgaaa aaaactggtt gctgttttat aaaagcgact gtaacaacct ccactctata
     4501 gttccattag tttaaagtgt cacacaaact gagcatccat ttgttttcct tacttttgcc
     4561 cctgtattcc aaggggtttt tttataactt aaagattagc acaccacaca aaaacaataa
     4621 aaaaaacgac aagttataca ataaaaccag tactatccat acagctaatt acatataaat
     4681 actattaaac catctggaca ttaaaaataa actcttataa cataaaatta atgcggttcc
     4741 ggtaccatat gtcagggata atgtgcaaat ggaggtatcg gaactgcatt tcagttcatt
     4801 attaattaat gacaaaaaaa aacctctgcg agcgcagagg gggtaaggaa tgttcccggt
     4861 cataatatgt cgatagtgtt tatatacata cagaacaaga acaacaacca gccaactaat
     4921 aaatatttta ttttttagag ctgaaacctt aaagctcact tccattccat acattgttat
     4981 aaaccttatg ttgaaaactg aaagtaagta cggcagactt attaagttgc gtgcagtctt
     5041 ttcacgttat acaaacaaga acacctgcga agttatttct ccttacatat ccccctatgc
     5101 caacggggga ttttttctgg ttcactaacg cataaataat agtgacaccc tatcattcac
     5161 aaaccgt
//
