LOCUS       CP088672                5145 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain C16EC0292 plasmid pC16EC0292-4, complete
            sequence.
ACCESSION   CP088672
VERSION     CP088672.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391598
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 14:05:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,363
            CDSs (total)                      :: 5,247
            Genes (coding)                    :: 5,045
            CDSs (with protein)               :: 5,045
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 80 of 202
            Pseudo Genes (incomplete)         :: 129 of 202
            Pseudo Genes (internal stop)      :: 42 of 202
            Pseudo Genes (multiple problems)  :: 45 of 202
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C16EC0292"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pC16EC0292-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.34 N 127.92 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(5014..5145,1..267))
                     /locus_tag="LQT17_26795"
     CDS             complement(join(5014..5145,1..267))
                     /locus_tag="LQT17_26795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGH77338.1"
                     /translation="MRISNIEWLKKRIEFIRKLGKQTARQRQIIDLLDNEDRLTEQERK
                     LLHVLATAEKNDLQAQESERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDMLLQRLKPIKYDLDTCPNPDA"
                     /name="hypothetical protein"
     gene            444..1370
                     /locus_tag="LQT17_26800"
     CDS             444..1370
                     /locus_tag="LQT17_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGH77339.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDIAREHGFD
                     MRDGVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1373..1906
                     /locus_tag="LQT17_26805"
     CDS             1373..1906
                     /locus_tag="LQT17_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGH77340.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISLDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1972..2181)
                     /locus_tag="LQT17_26810"
     CDS             complement(1972..2181)
                     /locus_tag="LQT17_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGH77341.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLRKLPPDHTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            3913..4821
                     /locus_tag="LQT17_26815"
     CDS             3913..4821
                     /locus_tag="LQT17_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGH77337.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGRSIAKYTHRKFSPEGFSAVQAARGRKGGIAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     Typing          3914..4641
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.61"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          445..1367
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcgagaaag agccggtgat tgcgttcccg tcgttgtttc ttcccctcga ttcgtttctg
       61 gatcgcctgt tttcgttcgc tctcctgtgc ctgtaagtcg tttttttcgg ctgttgctag
      121 tacgtggaga agtttgcgtt cctgttctgt gagtctgtcc tcgttgtcga gcaggtcaat
      181 gatctgtctc tgtcgtgcgg tctgttttcc gagcttcctg atgaactcga tccgtttctt
      241 cagccattcg atgttgctga tgcgcatgtt ggtggtgctc cctggtgttt ttttgcggtg
      301 tgttgtggtc gtgcagtata tcgcttgcgg tcggtgggtg caggggttac cttccgtact
      361 aattcgaaaa attagaagga agcgcactta tatgtttctc tccttcgttg agaaacagtg
      421 cgctcctgcg gagggctgaa accgtggcga cctatcacct cagcgttaaa tttggtggca
      481 agggacaggc tgccaaccat gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc
      541 aagatttgga atattccgca cacggaaaca tgccggaatg ggccagggat aatccttccc
      601 atttctggca ggcggctgac cagtttgaac gtgcgaacgg ttcgacctat agggaacttg
      661 aaattgcatt accccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg
      721 tacgtcagga agctggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga
      781 gcattgacgg cggcgaacag cctcatgcac atatcatgat gagtcagcgt gtgaatgatg
      841 gcattgaacg tacaccggaa cagtatttcc ggcgctataa cgccaggtat cccgaccggg
      901 gaggtgcgaa aaaggattcc ggaaacctga cccccactca gcaaaaagag cagttgcggg
      961 aattgcgaaa acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa
     1021 ggggcttcat tgactgccgg acgctgaaag aacagggcat tgaacgacgt cccgaagtgc
     1081 atctgggttt tgaagctgcc ggacgacttg atgatgccca gcgtcatgac atcatgcaaa
     1141 aacggtctga accggactat taccgccatc ttgaacaggc gcgaatggtc gggcatgaca
     1201 tcagggaatg gctggataca ccggttaagc aacatgaact ggtcagaagg ctgcaacatg
     1261 atattgcgcg ggaacatggc tttgacatga gagacggagt gagcagacag aaccgaactg
     1321 aacctgaacg ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag
     1381 cacagaagaa aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat
     1441 ggagcaacag aaagctgttg ctgattctct ggctgtggca agggaaacgc ttcgcggata
     1501 tcgggatatt tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa atgaggtgca
     1561 gaagctggac gtagcagggc tgataggtga gcgcatcagc ctggattttc agcggctgga
     1621 agagtcagtc gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat
     1681 tcacagcaat atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg
     1741 tggtgtgtca gtggcttttc tggttatgtt ctgtgtgctc ggatggggaa tgaaaacaat
     1801 gctcgacagg aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga
     1861 taaactggcg gagaaacctg tcacgggtaa gagccggaaa aactgacgcg cttcgcttgt
     1921 ccaacgcctc acggagcgcg aaaggatttc gcgaaccgtt caccgttttt ttcaatatga
     1981 ttctgtctgc tttggtcgat aaagcagtcg atagtattcc agccttcgta tatataacgg
     2041 tacgtgctca tcagtaagat ctggcggtat tgtgtgatca gggggaagtt ttctcagata
     2101 ctcacgaaat gcctggcttg atgccataaa atctacagta gcatcgataa tatgtggggt
     2161 gttttcacct aattttgcca tgatgataat ccttatatta tcacggttcg tgaatgatag
     2221 ggggtcacta ttatttatgc gttagtaaac cagaaaaaat cccccgttgg cataggggga
     2281 tatgtaagga gaaataactt cgcaggtgtt cttgtttgta taacgtgaaa agactgcacg
     2341 caacttaata agtctgccgt acttactttc agttttcaac ataaggttta taacaatgta
     2401 tggaatggaa gtgagcttta aggtttcagc tctaaaaaat aaaatattta ttagttggtt
     2461 ggttgttgtt cttgttctgt atgtatataa acactatcga catattatga ccgggaacat
     2521 tccttacccc ctctgcgctc tcagagggtt ttttttgtca ttaattaata atgaactgaa
     2581 atgcagttcc gatacctcca tttgcacatt atccctgaca tatggtaccg gaaccgcatt
     2641 aattttatgt tataagagtt tatttttaat gtccagatgg tttaatggta tttatatgta
     2701 attagctgta tggatagtac tgtttttatt gtataacttg ttgtttttta ttgtttttgt
     2761 gtggtgtgct aatctttaag ttataaaaaa accccttgga acacaggggc aaaagtaagg
     2821 aaaacaaatg gatgctcagt ttgtgtgaca ctttaaacta atggaactat agagtggagg
     2881 ttgttacagt cgcttttata aaacagcaac cagttttttt caatagtctg atgatggttg
     2941 tgacagtttt gtgatattgt tcaaaatgtt tcttgttgaa tgtttgtttt ttggtctatt
     3001 ggtgtctggt tttatttttt ctgctattac atatcaagaa atgtgattgg tgtagcaaga
     3061 gtggaatgcg atgtggataa actaattggt tgtcgaatct aatttactta gaaaactaca
     3121 cttgtttttg tgtatgaatt tagtgaattt gtctgtctat gtgttttcgt gcatttataa
     3181 atccttcgtc aggatcttaa atttagtgtt gaatatgtag ggcctgtacg gtaattatta
     3241 tttagcgtag ctacttcatc gttgttcgaa cacaaccaac aacctgtaat tgttgtttct
     3301 cttggttccc cacttttgtg gggtttttta tctcttccgt gcatcgtaaa gcgcggactc
     3361 cttctggaaa ggttgtttta gggcatcact gaggcatttt ttgcgtttgt gaatattgag
     3421 catccgataa ctaaaatatt tcccatagac tctgattctt gccaccgacc ccaggtcggt
     3481 tttttttgtc ctggcgagat gtgaagtttg gaaacaggaa accccgtcag agcgccacgc
     3541 accaggggag aaaacttgtc gttttgagcc gttcggtgtt cagaacgcac gaaaccgatc
     3601 gcacatcgct ttcgtgcata gttatgcaac gccctgaaaa cgattctgac gcatttttct
     3661 ggttcagcct ggtgtttcct tgtctttttg cgttttttgc gtcagaaagc gattgagggc
     3721 gttttatggg gtgcgtacaa cgggagttat ggtaaatgga tcggatttgc gggaaggctc
     3781 gacaggtttt gtcgttgggg gtagtgtaag cgactgaaaa acaaacgccc ctgaaattcg
     3841 tgtctatccg ccaagacttt cacgaaaata caggggcgat gtcggtcgat aacctggaag
     3901 gaatcgtaac acatgagcgc cgcgcttcaa tacttcgaag aaaatttacc ccaccgtccc
     3961 tatcacacgg atgatctcgc tttcggcctt cgtatctccg gcaaagggcg cgcgcttctt
     4021 gcacggtata tccagcagaa ccagcctcat gcacagttct ggctggtttt tgacgttgac
     4081 cgcgaggggg ctgcgattga ctggagcgac cggaacgctc ccgcgccgaa catcaccgtt
     4141 aaaaatcctg tgaacggaca cgctcacctg ctctacgcac tcaacatcgc cgtgagaacc
     4201 gcgcctgatg cctcggttaa ggcgctgaaa tatgccgcag cggttgaacg ttcgctgtgt
     4261 gagaaacttt gtgcggatgt gaactacagc ggcctgattt gcaaaaatcc gttccacctg
     4321 gaatggctgg tgatggagtg gcgcgaggaa gcctataccc tcgatgaact ggctgattat
     4381 ctcgatttga gcgcctcagc gcgccgtagc atcgataaac attacgggat ggggcgaaac
     4441 tgccacctgt tcgaaatgac gcgcaaatgg gcttacaggg cgattcgtca gggctggcct
     4501 gaattctcac agtggcttga tgccgtgatt cagcgtgtcg aaatgtacaa cgcatcgctt
     4561 cctgttccgc tttcaccagc tgaatgccgg gctattggca ggagcattgc gaaatacacg
     4621 catagaaaat tctcgccaga agggttttcc gctgtacagg ccgcacgcgg tcgcaagggg
     4681 gggatcgcta aaggcgaagc ctacgaggac aagcgtttca tggcgttatg tatgctggag
     4741 aatggatatt ctcagaaagc tattgcggcg atgctgaatg tacatcgtaa tacgatccgt
     4801 aattgggcaa tgcacaaata gccttatcag ataacagcgc ctttccggcg gctttttgag
     4861 cagctatttt ttgggtggtt tctgcttgtc agcccagcaa tggtgcggct tttcgcttga
     4921 ttgaggtggt agtgctcgcc gcagtctcat gaccgagcgt agcgagcgaa tgagcgagga
     4981 agcaccaggg caccggaacg tgaacgggaa cacttacgcg tctgggtttg ggcatgtgtc
     5041 tagatcgtac ttaatgggtt taagtctttg taataacatg tcttttttat agcatagctc
     5101 gcctgttttt gtgtccacca gtcctgcttc gatcagtagt cctgc
//
