LOCUS       CP088631                9264 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain C17EC0083 plasmid pC17EC0083-2, complete
            sequence.
ACCESSION   CP088631
VERSION     CP088631.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391614
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:52:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,475
            CDSs (total)                      :: 5,356
            Genes (coding)                    :: 5,064
            CDSs (with protein)               :: 5,064
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 292
            CDSs (without protein)            :: 292
            Pseudo Genes (ambiguous residues) :: 0 of 292
            Pseudo Genes (frameshifted)       :: 126 of 292
            Pseudo Genes (incomplete)         :: 214 of 292
            Pseudo Genes (internal stop)      :: 53 of 292
            Pseudo Genes (multiple problems)  :: 86 of 292
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C17EC0083"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pC17EC0083-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.34 N 127.92 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            31..527
                     /gene="tsaC"
                     /locus_tag="LQT83_27320"
                     /pseudo=""
     CDS             31..527
                     /gene="tsaC"
                     /locus_tag="LQT83_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /name="tsaC"
     gene            605..1409
                     /gene="aroE"
                     /locus_tag="LQT83_27325"
                     /pseudo=""
     CDS             605..1409
                     /gene="aroE"
                     /locus_tag="LQT83_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /name="aroE"
     gene            1423..1661
                     /locus_tag="LQT83_27330"
                     /pseudo=""
     CDS             1423..1661
                     /locus_tag="LQT83_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /name="DUF1488 domain-containing protein"
     gene            complement(1637..2189)
                     /locus_tag="LQT83_27335"
                     /pseudo=""
     CDS             complement(1637..2189)
                     /locus_tag="LQT83_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /name="gamma carbonic anhydrase family protein"
     gene            2660..4197
                     /locus_tag="LQT83_27340"
     rRNA            2660..4197
                     /locus_tag="LQT83_27340"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
                     /name="16S ribosomal RNA"
     gene            4266..4342
                     /locus_tag="LQT83_27345"
     tRNA            4266..4342
                     /locus_tag="LQT83_27345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4300..4302,aa:Ile,seq:gat)
                     /name="tRNA-Ile"
     gene            4384..4459
                     /locus_tag="LQT83_27350"
     tRNA            4384..4459
                     /locus_tag="LQT83_27350"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4417..4419,aa:Ala,seq:tgc)
                     /name="tRNA-Ala"
     gene            4641..7524
                     /locus_tag="LQT83_27355"
     rRNA            4641..7524
                     /locus_tag="LQT83_27355"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
                     /name="23S ribosomal RNA"
     gene            7629..7741
                     /gene="rrf"
                     /locus_tag="LQT83_27360"
     rRNA            7629..7741
                     /gene="rrf"
                     /locus_tag="LQT83_27360"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
                     /name="rrf"
     gene            7795..7868
                     /locus_tag="LQT83_27365"
     tRNA            7795..7868
                     /locus_tag="LQT83_27365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7829..7831,aa:Asp,seq:gtc)
                     /name="tRNA-Asp"
     gene            8031..8836
                     /gene="dkgB"
                     /locus_tag="LQT83_27370"
                     /pseudo=""
     CDS             8031..8836
                     /gene="dkgB"
                     /locus_tag="LQT83_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /name="dkgB"
     gene            complement(8846..9262)
                     /locus_tag="LQT83_27375"
                     /pseudo=""
     CDS             complement(8846..9262)
                     /locus_tag="LQT83_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing protein"
                     /name="LysR substrate-binding domain-containing protein"
ORIGIN
        1 gtggaaagcc gatttcggcg gaataataac gtgaataata acctgcaagg agacgctatc
       61 gcagctgcga tagatgtcct catgaagaac gtgtcatcgc ctatccaacg gaagccgttt
      121 tcggtgtcgg gtgcgatcct gatagcgaac agcagtgatg agactgttgg agttaaaacg
      181 gcgtccggtt gataagggct gattttaatc gcggcaaatt acgagcagct taaacccata
      241 ttgatgacac catgctgact gacgcgcagc gtgaaaccat tttttccccg ctggccaggt
      301 cctgtaacct ttgtctttcc cgcgcctgcg acaacaccgc gctggttgac gggccgcttt
      361 gattcgcttg ctgtacgagt cacgaccatc cgttggtggt tgctttgtgc caggcttatg
      421 gtaaaccgct ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag
      481 acgaagttcg cgcacaattt ggcgctgcgt tcccggtttg tgcctggtga aacggggggc
      541 gtttaaatcc ttcagaatcc gcgatgccct gacggtgtga actgttttcg acaggggtaa
      601 cataatggaa acctatgctg tttttggtaa tccgatagcc cacagcaaat cgccattcat
      661 tcatcagcat ttgctcagca actgaatatt gaacatccct atgggcgtgt gttgacaccc
      721 aatgatttca tcacacactg aacactttct ttagtgctgg tggtaaaggt gcgaatgtga
      781 cggtgccttt taaagaagag gcttttgcag agcggatgag cttactgaac gggcagcgtt
      841 ggctggtgct gttaataccc tcaagcggtt agaatatgta cgcttgctgg gtgcaatacc
      901 gatggtatag gtttgttaag cgatctggaa cgtctgtctt ttatccgtcc ttgtttacgt
      961 atttgctcat cggcgctggt ggagcatctc gcggcgtact actgccactc ctttccctga
     1021 ctgtgcggtg acaatcacta tcggacggta tccgcgcgga agagttggct aacttgtttg
     1081 cgccactggc agtattcacg cgttgggtat gcacgaactg gaaggtcatg agtttgatct
     1141 cattattaat gcaacatcca gtgcatcagt ggtgatattc cggcgatccg tcatcgctca
     1201 ttcatccagc atttattgct atgacatgtt tctatcagaa aggaaaaact ccttttctgg
     1261 catggtgtga gcagcgaggc tcaaagcgta ctgctgatgg ttaggaatgc tggtggcaca
     1321 agcggctcat gccttccttc tttggcatgg cgttctgcct ggatgtagaa ccggtcatca
     1381 aactattgca acaggagttg tcagcgtgaa tcaggccatc catttccgga tagggaagag
     1441 tgggacgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggtat gcaactgaca
     1501 tgtgcgatct ctggcgagag tctggcgtat cgctttactg gagatacacc agaacagttg
     1561 gttagagagt tttcgtcagc atcgctggga cctggaagaa gaagcggaaa cttaattcag
     1621 gaacaaagtg aaatgatcaa ggttgggtgt gtttaccctg atccatatat tcgtccttcc
     1681 atttcacgta gttatttgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc
     1741 ggatctgttt gacgggctac cgagtacaga tatccgctct ccagccgttt attttgtggg
     1801 accaggctac ctgcaccaat catcacatca tcttctacta ttgcgccatc aagtaaaatg
     1861 agcccatccc aacacaaaac tcgattgcca tggtgcagcc gtgggcatca ccttgtgacc
     1921 aatagtgaca tcttcgccaa tggttataat gggttgccag ctgggtttgt acgaggattt
     1981 atgatgacgt gcaacatact gccatcctgg atattggtgc gtgctccgat ctgtacataa
     2041 tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc cagacgaacg
     2101 tcaccaatca cgacgctgct atcgtcgatc attacgctgc tgaccgattt gtggaaaaag
     2161 atcgcgtatg ggcgtaaaac ctcagacata ctcacctcaa caataaatga ttttactaat
     2221 gactttgggt acattattgg ccttgtgcaa gtcttttagt atgcaaaaaa agcaccgttt
     2281 tgtgtgcgaa tgcagcaaaa agggtgaaaa aacaacaaca gaaaaaaaag atcaaaaaag
     2341 tacttgtgca aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaca
     2401 cttcacaaga tagtcgtaac aacaagagaa aaatcctgaa aattcagggt tgactctgaa
     2461 agaggaaagc gtaatatacg ccactcgcga cagtgagctg aaaaccgcgt cgcaactgct
     2521 ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc ttgacgtcgc
     2581 aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat
     2641 tcacgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
     2701 gcaggcctaa cacatgcaag tcgaacggta acaggaagca gcttgctgct ttgctgacga
     2761 gtggcggacg ggtgagtaat gtctgggaaa ctgccgatgg agggggataa ctactggaaa
     2821 cggtagctaa taccgcataa cgtcgcaaga ccaaagaggg ggaccttcgg cctcttgcca
     2881 tcggatgtgc ccagatggga ttagctagta tgggtgggta cggctcacct aggcgacgat
     2941 ccctagctgg tctgagagga tgaccagcca cactggaact gagacacggt ccagactcct
     3001 acggaggcag cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg
     3061 tgtatgaaga aggccttcgg gttgtaaaag tactttcagc ggggaggaag ggagtaaaag
     3121 ttaatacctt tgctcattga cgttacccgc agaagaagca ccggctaact ccgtgccagc
     3181 agccgcggta atacggaggg tgcaagcgtt aatcggaatt actgggcgta aagcgcacgc
     3241 aggcggtttg ttaagtcaga tgtgaaatcc ccgggctcaa cctgggaact gcatctgata
     3301 ctggcaagct tgagtctcgt agaggggggt agaattcagg tgtagcggtg aaatgcgtag
     3361 agatctggag gaataccggt ggcgaaggcg gccccctgga cgaagactga cgctcaggtg
     3421 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc
     3481 gacttggagg ttgtgccctt gaggcgtggc ttccggagct aacgcgttaa gtcgaccgcc
     3541 tggggagtac ggccgcaagg ttaaaaactc aaatgaattg acgggggccc gcacaagcgg
     3601 tggagcatgt ggtttaattc gatgcaacgc gaagaacctt acctggtctt gacatccacg
     3661 gaagttttca gagatgagaa tgtgccttcg ggaaccgtga gacggtgctg catggctgtc
     3721 gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac gagcgcaacc cttatccttt
     3781 gttgccagcg gtccggccgg gaactcaaag gagactgcca gtgataaact ggaggaaggt
     3841 ggggatgacg tcaagtcatc atggccctta cgacagggca cacacgtgct acaatggcgc
     3901 atacaaagag aagcgacctc gcgagagcaa gcggacctca taaagtgcgt cgtagtccgg
     3961 attggagtct gcaactcgac tccatgaagt cggaatcgct agtaatcgtg gatcagaatg
     4021 ccacggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg ggagtgggtt
     4081 gcaaaagaag taggtagctt aaccttcggg agggcgctta ccactttgtg attcatgact
     4141 ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg gttggatcac ctccttacct
     4201 taaagaagcg tactttgaag tgctcacaca gattgtctga tgaaaatgag cagtaaaacc
     4261 tctacaggct tgtagctcag gtggttagag cgcacccctg ataagggtga ggtcggtggt
     4321 tcaagtccac tcaggcctac caatttgcac cgcaaatttg aagaggtttt aactacatgt
     4381 tatggggcta tagctcagct gggagagcgc ctgctttgca cgcaggaggt ctgcggttcg
     4441 atcccgcata gctccaccat ctctgtagtg attaaataaa aatacttcag agtgtacctg
     4501 caaaggttca ctgcgaagtt tgctctttaa aaatctggat caagctgaaa attgaaacac
     4561 tgaacaacga aagttgttcg tgagtctctc aaattttcgc gacacgatga tgaatcgcaa
     4621 gaaacatctt cgggttgtga ggttaagcga ctaagcgtac acggtggatg ccctggcagt
     4681 cagaggcgat gaaggacgtg ctaatctgcg ataagcgtcg gtgaggtgat atgaaccgtt
     4741 ataaccggcg atttccgaat ggggaaaccc agtgtgtttc gacacactat cattaactga
     4801 atccataggt taatgaggcg aaccggggga actgaaacat ctaagtaccc cgaggaaaag
     4861 aaatcaaccg agattccccc agtagcggcg agcgaacggg aggagcccag agcctgaatc
     4921 agtggtgtgt tagtggaagc gtctgaaagg cgcgcgatac aggtgacagc cccgtacaca
     4981 aaaatgcaca tattgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata
     5041 tggggggacc atcctccaag gctaaatact cctgactgac gatagtgaac cagtaccgtg
     5101 agggaaaggc gaaaagaacc cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta
     5161 caagcagtgg gagcctctta atggggtgac tgcgtacctt ttgtataatg ggtcagcgac
     5221 ttatttattt ctgtagcaag gttaccgaat aggggagccg aagggaaaac cgagtcttaa
     5281 ctgggcgtta agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg
     5341 aaggttgggt aacactaact ggaggaccga accgactatg ttgaaaaatt agcggatgac
     5401 ttgtggctgg ggtgaaaggc caatcaaacc gggagatagc tggttctccc cgaaagctat
     5461 ttaggtagcg ctcgtgaatt catctccggg ggtagagcac tgtttcggca agggggtcat
     5521 cccgacttac caacccgatg caaactgcga ataccggaga atgttatcac gggagacaca
     5581 cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc
     5641 aaagtcatgg ttaagtggga aacgacgatg tgggaaggcc cagacagcca ggatgttggc
     5701 ttagaagcag ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg
     5761 gaagatgtaa cggggctaaa ccatgcaccg aagctgcggc agcgacacta tgtgttgttg
     5821 ggtaggggag cgttctgtaa gcctgtgaag gtggcctgtg agggttgctg gaggtatcag
     5881 aagtgcgaat gctgacataa gtaacgataa agcgggtgaa aagcccgctc gccggaagac
     5941 caaggttcct gtccaacgtt aatcggggca gggtgagtcg acccctaagg cgaggccgaa
     6001 aggcgtagtc gatgggaaac aggttaatat tcctgtactt ggttgtgtta ctgcgagtaa
     6061 ggggggacgg agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc
     6121 tggttttcca ggcaaatccg gaaaaatcaa ggctgaggcg gtgctgaagc gacaaatgcc
     6181 ctgcttccag gaaaagcctc taagcatcag gtaacatcaa atcgtacccc aaccgacaca
     6241 ggtggtcagg tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa
     6301 atggtgccgt aacttcggga gaaggcacgc tgatatgtag gtgaagcgac ttgctcgtgg
     6361 agctgaaatc agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt
     6421 gcaaacacga aagtggacgt atacggtgtg acgcctgccc gtgccggaag gttaattgat
     6481 ggggttagcg gtaacgcgaa gctcttgatc gaagccccgg taaacggcgg ccgtaactat
     6541 aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacctgcac gaatggcgta
     6601 atgatggcca ggctgtctcc acccgagact cagtgaaatt gaactcgctg tgaagatgca
     6661 gtgtacccgc ggcaagacgg aaagaccccg tgaaccttta ctatagcttg acactgaaca
     6721 ttgagccttg atgtgtagga taggtgggag gctttgaagt gtggacgcca gtctgcatga
     6781 gccgaccttg aaataccacc ctttaatgtt tgatgttcta acgtgggccc cttaccgggt
     6841 tgcggacagt gtctggtggg tagtttgact gggcggtctc ctcctaaaga gtaacggagg
     6901 agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg gcataagcca
     6961 gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg
     7021 ttctgaatgg aagggccatc gctcaacgga taaaaggtac tccggggata acaggctgat
     7081 accgcccaag agttcatatc gacggcggtg tttggcacct cgatgtcggc tcatcacatc
     7141 ctggggctga agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct
     7201 gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg gagaactgag
     7261 gggggctgct cctagtacga gaggaccgga gtggacgcat cactggtgtt cgggttgtca
     7321 tgccaatggc actgcccggt agctaaatgc ggaagagata agtgctgaaa gcatctaagc
     7381 acgaaacttg ccccgagatg agttctccct gactccttga gagtcctgaa ggaacgttga
     7441 agacgacgac gttgataggc cgggtgtgta agcgcagcga tgcggttgag ctaacggtac
     7501 taatgaaccg tgaggcttaa ccttacaacg ccgaagatgt tttggcggaa gagacatcga
     7561 taagtaagct tgatagcaga tttaaatcga caggtcataa cgggacgtgt gtgataaaac
     7621 cgaatttgcc tggcggcctt agcgggtggt cccaccgacc ccatgccgaa ctcagaagtg
     7681 aaagcgcgta gcgccgatgg taggtggggt ctccccatgc gagagtaggg aactgccagg
     7741 tatcaaatta agcagtaagc cgtcataaaa ccggtggttg taaaagaatt cggtggagcg
     7801 gtagttcagt cggttagaat acctgcctgt cacgcagggg ggcgcgggtt cgagtcccgt
     7861 ccgttccgca cttactaaaa gcctcgagtt aacgctcgag gtttttttcg tttgtatttc
     7921 tattattgcc aaaatcgcaa aaaatcctct gcgttttacg ccatttttcc gcaacagtct
     7981 gaagcccata atcacctcag ttaacgaaaa tagcattaaa agaggcaatt atggctatcc
     8041 ctgcatttgg tttaggcact ttccgtctga aagacgacgt tgttatttca tctgtgaaaa
     8101 cggcgcttga acttgattat cgcgcattga taacgcacaa atctatgata acgaagccgc
     8161 agtaggtcag gcgattgcag aaagtggcgt gccacgtcat gaactccaca tcaccactaa
     8221 aatctggttg aaaatctcag caagacaaat tgaatcccga gtctgaagag agcctgcaaa
     8281 aattggcgta ccgattatgt tgattctgac gctaatccct ggccgtcacc aaacgatgaa
     8341 gtctctgttg aagagtttat gcaggcgctg ctggaagcca aaaaacaagg gctgacgcgt
     8401 gagatcggta tttccaactt tacgatccca ttgatgagaa aaagcgattg ctgctgttgg
     8461 cgctgaaaac atcgctacta accagattga actctctcct tatctgcaaa accgtaaagt
     8521 ggtcgcttgg gctaaacagc acccagtatc catattactt cctatatgac gctggcgtat
     8581 ggcaaggcac tgaagatgag gttattgctc gtattgcagc taaacacaat gcgactccgg
     8641 ctcaagtgat tctggcgtgg ctatggggga aggttactca gttaattcct tcttctacta
     8701 aacgtgaaaa cctggagagt aatcttaagg cacaaaattt tcagcttgat gccgaagata
     8761 aaaaagcgat cgccgcactg gattgcaacg accgcctggt tttagcccgg aaggtctggc
     8821 tcctgaatgg gattaagcct ctctcagctc ctccgggagc tgtttttaca tgctcgctaa
     8881 ggaaatcgat aaaagcccgg atgcgcgtac ttacgcgcac ggtcgctgta atagactgca
     8941 ctgaatggca tttctactgg caacacttta ctgctattaa ctccaccaat tctccagagc
     9001 gatttctttg cgcatcatgt agtcggacaa acacgcaatc ccgtttgcca ctcaggcaaa
     9061 gctgtttcag tgtttcccca ctattgatga caaaccgtac ttaacctcat gtaattgtcc
     9121 atcgctacag gctatcggcc aggtattgag gaagcgggtt cagtgaatcc caggcaaata
     9181 tgttgcttta aatcgtcgat cgtttctggc ttcccgtagc gggaaatata tcgggggagg
     9241 cgataatttt tcgataactg ttaa
//
