LOCUS       CP088614                6760 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain C17EC0299 plasmid pC17EC0299-1, complete
            sequence.
ACCESSION   CP088614
VERSION     CP088614.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391620
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 412x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 12:21:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,829
            CDSs (with protein)               :: 4,829
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 73 of 190
            Pseudo Genes (incomplete)         :: 135 of 190
            Pseudo Genes (internal stop)      :: 28 of 190
            Pseudo Genes (multiple problems)  :: 41 of 190
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C17EC0299"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pC17EC0299-1"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.34 N 127.92 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(6335..6760,1..1440)
                     /locus_tag="LQT57_24200"
     CDS             join(6335..6760,1..1440)
                     /locus_tag="LQT57_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="UGG90571.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKAL
                     VDNEKVRSVYGVDIDGIFIFDFDKLCGLLKYKEYWANSFTKYRNKVGLTSEGKYLDYSS
                     DVVLDFPFKDCILEGGMTKENQGKDEIYYNEIIAMDEVDSLLSPKAFTNAKRYTKEGVK
                     ENITKFNDDENLIIKGNNLIALHSIKKLYAGKVKLIYIDPPYNTGSDSFKYNDRFNRST
                     WLTFMKNRLEVAKEMLSDDGFIFIQCDDNESAYLKVLCDSIFGEENYRNSIYWHRTYAG
                     KTVKSKLPWNTDTILFYSKKESSLLEPYTAELSAADIKSYNKDDFDGRGKYNTVSLQKT
                     SSPGPQTTYDYIDNTGKKWSCPEKGWRMTKEKLKALENDNRLYITDKTIREKYYLKERK
                     EIGKQIDNFWHDIGNLNRVSDVAFIDGQKTEKLMQRIISLASKENDIVLDFHLGSGTSA
                     AAAHKMGRRYIGIEQMDYINEITVPRLQKVIEGEQGGISKDVNWQGSGSFVYVELVELN
                     AYFIHEIQKAQSTEELEKLFAVMKTEAYLNYQVALENVLSAEYEVDGILHKVAFSELEL
                     HEQKQLLIEILDKNQLYVNVSDMEDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            1445..4081
                     /locus_tag="LQT57_24205"
     CDS             1445..4081
                     /locus_tag="LQT57_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="UGG90572.1"
                     /translation="MAKKPKSAGREKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDIL
                     RPYQHSALRYLHYTQRKRDDALLHYRHLLFHMATGAGKTMVMAGTILYLFKEFGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSHELEIDGEKITIAPVDIFPSVPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEEQTWE
                     RTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDSDKMLDAVLLSQYRKLTAADHGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESIRRHLANKKLQLSSDTSIWHKVIQRYADSDLVTVTGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTSTDSEAQL
                     IGRGARYYPFIYDGQRSFTRRFDNSTKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTVEHARLKEDFKKSAVYQAGKLYFNEVEEIERSSRNWETYSLETRFEIQYQI
                     ACEESLDNLTGTKGGIIKPEPLVLDERFYRKAMQRISFYALDNLKRFFPKLTGIREFIR
                     SDAYLGKLKITVSVPQSLDFTTVPAKDKLHLLENVLLRISENVRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVAINQKITAAPTIGKKWYVYDNAILNQLEHRLIKMLEAFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNTYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEHVRLVGIKFFANSQMDVFVSDMQNKLNDGESLET
                     SSLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(4483..4596)
                     /locus_tag="LQT57_24210"
     CDS             complement(4483..4596)
                     /locus_tag="LQT57_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGG90569.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(5261..5455)
                     /locus_tag="LQT57_24215"
     CDS             complement(5261..5455)
                     /locus_tag="LQT57_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UGG90570.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5096..5181
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          5492..5590
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          5715..5970
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.248"
                     /coverage="89.0"
                     /evalue="2.69e-98"
     Typing          complement(4525..5078)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.075"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gactctttgc tttctcccaa ggcgttcact aatgctaagc gatataccaa agagggggta
       61 aaagaaaata tcactaaatt taacgatgat gaaaatttaa taattaaggg aaacaatctc
      121 attgcactac attcaataaa aaaactttac gcaggaaaag taaagttaat ttatatagac
      181 ccgccttaca atacagggag tgatagcttt aagtataatg atcgattcaa tcgctcaaca
      241 tggttgacat tcatgaaaaa taggcttgag gtggctaaag aaatgctttc cgatgatggc
      301 tttattttta ttcaatgcga tgataatgaa tcagcatatc ttaaagtttt atgtgattca
      361 atttttggag aagagaatta tagaaactca atctattggc atagaaccta cgcagggaaa
      421 actgtaaaaa gtaagttgcc atggaataca gatacaatat tgttttattc taaaaaagaa
      481 agttcattat tagagccata tacagcagaa cttagtgctg ctgacattaa atcctataac
      541 aaggatgatt ttgatggaag gggtaaatac aacactgtta gcttgcaaaa aacaagctct
      601 ccagggccgc aaacaactta tgattatata gataatacag gtaagaaatg gagttgccct
      661 gaaaaagggt ggagaatgac aaaggaaaag ttaaaggctt tggaaaacga caataggcta
      721 tatatcactg acaaaacaat acgagaaaaa tattatctta aagaaagaaa agagatcggg
      781 aaacaaatag ataatttttg gcatgacatt gggaatttaa atagggtttc cgatgttgct
      841 tttattgatg gacaaaaaac tgaaaaatta atgcaacgga taattagcct tgcttccaaa
      901 gaaaatgata tcgtcttgga ctttcatctc ggcagcggaa ctagcgcagc agcagcccat
      961 aaaatgggac gtcgctatat cggtattgag cagatggatt acatcaatga gatcaccgtg
     1021 ccacgtttgc aaaaagtcat tgagggtgag cagggaggta tctcaaaaga cgtcaactgg
     1081 caggggagcg gcagctttgt ctacgttgaa ctagtggaac tgaacgctta cttcatacat
     1141 gaaatccaga aagcgcagtc cacggaagaa ctcgaaaaac tctttgccgt gatgaagaca
     1201 gaggcatatc tcaactatca ggtggctctg gaaaatgtgc tgtccgctga gtacgaagtg
     1261 gatgggatcc tccataaggt agcattcagt gaactggaac tgcatgagca gaagcagctt
     1321 ttgattgaaa tcctagataa gaaccagctc tacgtgaatg tctctgatat ggaggacagc
     1381 gacctgaaca tttcagagag tgataaagcc tttacccgca gcttctacgg aatggagtga
     1441 cggcatggcg aagaaaccga agtcagcagg tagagagaag ctgctgttta acaagctgaa
     1501 gcagttcgat gaaccaggac tgttccacga taactatcag acgccggact atattcaggc
     1561 aaacctgaaa gatatcctgc gtccctatca gcacagcgca ctgcgttacc tgcattacac
     1621 gcaacgtaaa cgtgatgacg cgcttctgca ttaccgtcac ctgctgtttc acatggcaac
     1681 cggtgcaggg aaaacgatgg ttatggcagg aacgatactc tatctattta aagagttcgg
     1741 gtatcagaac ttcatctttt tcgtacatac cgacgcgatt atccagaaaa cgcgagagaa
     1801 cctacttaac ccgcaatcgc cgaagtacct gttcagtcat gagctggaaa tcgacgggga
     1861 aaaaatcacc atcgcaccgg tagatatatt cccgtccgta ccggaacgca acacgattta
     1921 cctcaagctc tcgactatcc ataagatgca cgatgagctg aacagctatc gagagaacag
     1981 cattacctac gaggatttaa aagaaatccc gcttgtgcta ctcggtgacg aggcgcatca
     2041 cttcaacgcc ggcaccaaag ctagaggcaa gtcgaaaacc tcaccggaga acgaggagca
     2101 gacttgggag cgtacgattg agaacatcct cgacctgcga ccggataacc gcctgttcga
     2161 gttcaccgct accattgacc ttgcaaacaa agatatcgga cagaaatacc gtgacaaggt
     2221 ggtatatcag tatgatctga agcagttcat gtcagacggt tactcgaaaa aggtcatgct
     2281 gttggaagca aaccagaacg acagcgacaa gatgctcgat gcggtactgt taagccagta
     2341 ccgtaaactg acggcagcag atcacggcat caccggcttt aaaccggtca ttctgttcaa
     2401 gtcgaataag attgcgatat caaaggcgaa gcaggaggag ttttcgcaac tgattgccgc
     2461 catgacacct gagagcatca gacgtcatct ggctaacaaa aaactccagc tcagctctga
     2521 caccagtatc tggcataaag tgattcagcg ttacgctgac agtgatttag tgacggtgac
     2581 tggacaaatt caggaggact ttaacgactt taacctgctg aacgtcaaca agtcagacct
     2641 gctggaggaa aaccccgtcc tgctgaatac actggaagaa gtcgataatc cagtgagagc
     2701 cgtctttgct gtcgcgaagg tgaatgaagg ctgggacgta cttaacctct acgatattgt
     2761 tcgcataagc gagcaggcga gcagcagcaa aaccagcacg gacagcgaag cgcagcttat
     2821 tgggcgaggt gcgcgttact acccgttcat ttatgacggt caacgtagct tcacccgccg
     2881 ttttgataac agtacgaagg atttgagcgt actggagcaa ctgcactatc acaccatcaa
     2941 tgaaccggcg tacatcaaaa cactgcacgc ctctctggag caggcggaca ttgacgtgca
     3001 tcaggatggc ggaggtacgg ttgaacacgc cagattgaaa gaagacttta aaaagtccgc
     3061 cgtgtatcag gcgggtaagc tgtactttaa cgaagtggaa gagattgaga gaagcagccg
     3121 aaactgggaa acctactcgc tggaaacacg ctttgaaata cagtatcaga tcgcttgcga
     3181 ggagtcgctg gacaacctga caggcacaaa gggggggatc ataaaaccgg aaccactggt
     3241 actggacgag cgtttttacc gcaaggcgat gcaaagaatc agcttttacg cgctagataa
     3301 cctgaaacgc ttcttcccga aactgacagg tattcgggag ttcatacgca gtgacgccta
     3361 tcttggtaag ctgaaaatca ccgtcagcgt accgcagtca ctggatttca ccaccgtacc
     3421 ggcaaaagat aagctgcacc tactggaaaa cgttttgctg cgtatctcag agaatgtgcg
     3481 tcgcaatgac cagaaggtaa aaggcacgta ccgatttatc agccagccgg tgaaagaggt
     3541 gattaaagat tacagtttgc atattgaccc gtcggtagcc attaaccaga aaatcaccgc
     3601 cgctcccacc attggtaaaa aatggtacgt gtacgataat gcgatactaa atcagcttga
     3661 gcatcgtctg ataaaaatgc tggaagcatt catgccgaag ctaaaagcgc gttatgacga
     3721 tatttacgta ctgcgtaacg acgagcagtc aacgcgcttt aagctcacgg agttcggcgg
     3781 cgtgcgtggg tttatgccag atttcatcat gatactgacg cgccattcag acaacactta
     3841 ctggcaggtg ttccttgaac cgaaggggga tgaccgtctg ctggatgacg catggaaaga
     3901 acggatgctc gaaacactga atgacaggga gcgcattgta attgatgaaa atgagcacgt
     3961 cagactggtt gggattaaat tctttgccaa tagccagatg gacgtttttg tcagcgacat
     4021 gcagaacaag ctgaatgatg gagaatcgct cgaaacctcc tcgctctccc taccactgta
     4081 agtcacgaca ataaaataac ggagcattac gctccgttat tttattgtta tctcgaacta
     4141 cctcccaggg aggtgatgat tgctcctgta atggttgcat tgtttgttct tgctgttgcg
     4201 aatcattagt tttgaatggt ggttaaaaac cgttcgctat gtttatgcct ggggggggga
     4261 tgagggggaa aagattcaag gatcctcttg tgatcctttt tttctgcgcg taatctgcag
     4321 cctgtaaacg aaaaaaccac cctggcaggt ggtttttcga aggttagcta atcctggcag
     4381 attatctaac cgggtaactg gcttggagga gcgcagtcac caaaatctgt tctttcagtt
     4441 tagccttaac aggtgcataa cttcaagaca aactcctcta aatcagttac cagtggctgc
     4501 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4561 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4621 ctacaccgaa ccgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4681 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4741 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc gactctgact
     4801 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg
     4861 cgcggccttt ttccggttcc tgctcttttg ctcacatgtt ctttccggcc ttatcccctg
     4921 attcggtgga taatctaatt actgtctttg cgtgagttga taccgctcgc cgcagccgaa
     4981 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgtcggaag ttgcattttc
     5041 tccttacgca tctgtgcggc atttcacatc ggttatggtg tacttttcat acaatctgca
     5101 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     5161 tgcgccccga cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa
     5221 aacgcccacg ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt
     5281 cggcctcaaa gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt
     5341 cgcacatggc cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc
     5401 tctggctttt gataaatcgc gccatattca gcgcagtttg ttgttgtttg ttcaccttcc
     5461 gttgttctcc gtcagttccg ggtgtcgggg ggaccctgag caggtggtaa ttgtaagacg
     5521 ttgcgggcga cggtattaca attgcacatc ctgtcctgtt tctttaggac gtatcatggc
     5581 acagcatatc aactgataaa taatccctgt gcagtgcagc cgctgttgca taaaatttcg
     5641 ctctgcgtcg agtgtttctt aatcaataat tccgctgata ctccggaaaa acgcgagtct
     5701 gttctcatgc ctgaaatgcc cacaccccac gcaaaaacaa gtttttgctg atttttcttt
     5761 ataaatagag agttatgaca aattagttct tcttgctctc tttgtgatat ttaaaaaagc
     5821 ggtgttggcg cagctacaac accgcgccga caccgctttt taggggtggt actgactatt
     5881 ttcataaaaa acatcatctt atattagggg tgctgctagc ggcgcggtgt gtttttttac
     5941 aggacgcccc tgggggcgct gctaggggtg tctgttcaga tatgtagcaa tgagtcaggg
     6001 attcacgaac tgaaaatgtc cgaaaatgtg tctcatgggt actgtatgcg cccaaaccga
     6061 tcgtcattca ctttcatgca tagctatgca gtgagctgaa agtgatcctg acgcattttt
     6121 ccggtttacc ccggggaaaa catctctttt tgcggtgttt gcggcagaat cgcgttcagc
     6181 gcgttttagc ggtgcgcgca agagcgttta tgttaaatac tactcgttct ggtgcatttt
     6241 cccgcaactg aatccaactc atcgctgttg ggttccagcg ccgaacgcta taccttacaa
     6301 tcattccaat atttaacagt cataaggcca tgacatgcag accgaattaa taaaagaact
     6361 taacaaggtt ttaaaagcgt tcccgcaatt ctggaatgga gaggaattac aacgttcaat
     6421 ggtgagtgac gctatcagtc aaaagcagcc agatcttatc aaggcattag tcgataatga
     6481 aaaagtccgc tctgtttatg gtgtggatat tgatggcatt tttatctttg attttgacaa
     6541 attatgtggt cttctaaaat acaaagaata ttgggctaat agctttacta aataccggaa
     6601 caaagtggga ttaactagcg aaggtaaata ccttgattac agttctgatg tggtgctgga
     6661 ttttcctttc aaggattgta ttcttgaagg tggaatgacc aaagaaaatc agggaaaaga
     6721 tgagatttat tataatgaaa tcatagcaat ggatgaagtt
//
