LOCUS       CP088517                6481 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain E16EC0790 plasmid pE16EC0790-1, complete
            sequence.
ACCESSION   CP088517
VERSION     CP088517.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391651
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:42:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,740
            CDSs (with protein)               :: 4,740
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 86 of 217
            Pseudo Genes (incomplete)         :: 138 of 217
            Pseudo Genes (internal stop)      :: 37 of 217
            Pseudo Genes (multiple problems)  :: 37 of 217
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E16EC0790"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pE16EC0790-1"
                     /geo_loc_name="South Korea"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(4868..5500)
                     /locus_tag="LQQ83_24330"
     CDS             complement(4868..5500)
                     /locus_tag="LQQ83_24330"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="UGF51247.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTAL
                     ISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALTKQ
                     LKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQELR
                     SAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDME"
                     /name="DUF262 domain-containing protein"
     gene            complement(5785..6186)
                     /gene="pemK"
                     /locus_tag="LQQ83_24335"
     CDS             complement(5785..6186)
                     /gene="pemK"
                     /locus_tag="LQQ83_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="UGF51248.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGT
                     RPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTID
                     MKARGGKRLERVPETIMNEVLGRLSTILT"
                     /name="pemK"
     gene            complement(6119..6376)
                     /gene="pemI"
                     /locus_tag="LQQ83_24340"
     CDS             complement(6119..6376)
                     /gene="pemI"
                     /locus_tag="LQQ83_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="UGF51249.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
                     /name="pemI"
ORIGIN
        1 tctgaagtaa actataaaaa tttatgtatc ttatattatt ctctgtatat attctctatt
       61 attattattt tatttattta tattttttct ataattctct attcttttct ttatattctc
      121 tctctttcat ttatattatt tattttttat ctttattctt ttatttcttt gattcttttt
      181 ttttatttca ttcttatatt tttctctctt atttttcttt atcttttctt tttctctttt
      241 ttttttttct tttttttttt attttcttct tttctttttt ttttccattt tttttatttt
      301 tcattttttt tgttcttttt ttttcttttt tttttttttt ttttttttat ttttttttgt
      361 ttttctctat tttttttttt ttctttattc tcttacattt tttttttttt tttttttttt
      421 cttttttttt ttttcttttt tttttttttt ttttcttttt tttttttcct tttcttgttt
      481 ttttttcttt tgttgttttt ttttttcttt tttttttttt tttttctttt tctctttttt
      541 tctttttttt tctttttctt tctttttctt ttttttcttt ttttttcttt tctttttttc
      601 tctctttttt ttcttttctc tctctctctt ttctttttct tttttctttt tctttttttt
      661 tcttcttttt tcttttcttt cttttttttt ctttttcttt ttttttctct tttttctttt
      721 ttttcttctt ttttttctct tctttttttt tctttttctt tttttttctt tttttctttt
      781 ttcttttttc ttttttctct ctcttttctt tttttctttt ttcttttttt cttttctttc
      841 tttttttttc tttttttctt tctctttctt cttttttttt cttttttttc tttttttttc
      901 tttctttttc tcttttttct tttttttctt tttttttctc tttttctttt tctttttttt
      961 tctttttttt cttttttttt cttttttttc ttttttcttt cttttctttt tttctcttct
     1021 cttttctttt tttttctttt tctctttttt ctcttttttt ctttttcttt tctctttctc
     1081 tctctttttt ctttctttct tttctttttc tttttctctt ttttctttct tctttttttc
     1141 ttcttttctt tttttttctt tttctttttt ttctttcttt tttctttctt ttttctcttc
     1201 tttttttctt ttttcttctt ttttcttctt cttctttttc ttttcttctt ttcttttttt
     1261 ttctttcttt tctttttctt tctttttttc ttctttttct tttttttctt tttctttctt
     1321 tttctttctt ttttttcttt ttttctttct ttttttcttt tttcttcttt ttttttcttt
     1381 ttctttcttc tttttctttt tttttctttt cttcttttct ttttttcttt ctttctttct
     1441 ttttcttttt ttcttctttc tttcttcttt tcttttttct tcttttcttc tttttttctt
     1501 tctttttctt tttctttctt ttttttcttc tttttttctt cttttctttt cttctttctt
     1561 tttttttctt ttcttctttc tttctttttt ttcttttctt tctttttctt ttctttcttt
     1621 tcttttcttt ttcttctttc tttctttctt ttcttttttc ttttttcttt ttttcttttt
     1681 tcttttcttt tttctttttc tttttctttc ttttttcttc ttctttttct tcttttcttt
     1741 ttctttcttc tttttctttt tcttcttttc tttcttttct ttttttttct tttcttttct
     1801 tcttttcttt ctttttcttc tttctttctt tttttttctt ttctttcttt ttctttcttt
     1861 ttttcttttt tctttttctt ttcttttctt ctttcttttt tcttttcttc ttttttcttt
     1921 tctttcttct tcttttttct tttcttcttt tcttttcttc tttttcttct ttttctttct
     1981 ttttttcttt ctttcttttc tttttctttc tttctttttt tcttttcttt ttcttttctt
     2041 cttttttctt ttcttttttc tttttttctt tcttcttttt tctttttttc ttcttctttc
     2101 ttttctttct tttttctttc tttttctttt tcttcttttt tctttcttct ttttttcttt
     2161 ttcttttttc ttttctttct tcttttttct tttctttttt tctttctttt tcttttttct
     2221 ttcttttttc ttttttcttt ttcttttttc ttttcttctt ttttcttttt cttttttctt
     2281 tcttttcttt tcttctttct tttttcttct tttctttttc tttctttttc ttttcttttc
     2341 ttttttcttt ttctttcttc tttttctttc ttttttttct ttctttttct tttcttcttt
     2401 cttttctttt ttcttttctt ttttctttct tcttctttct tttttctttc tttttttttc
     2461 ttttcttttc tttttcttct ttttttcttt tttcttttct tttctttttt tttctttctt
     2521 tttctttttt tctttctttt tttctttttc tcttctttct tttttctttt tcttttcttt
     2581 tcttcttttt tctttttctt tttttctttt tctttttttt tctttctttt tttttcttct
     2641 ttcttttctt tttttctttt ttcttttctt tcttcttttt tctttttttt tctttttttc
     2701 tttctttttt ttctttcttt ttcttttctt tttttttctt tctttttttt tcttttcttt
     2761 ctttttcttt tctttttttc ttttttcttt tcttttttct ttcttctttc ttcttctttt
     2821 tttttcttct ttttttttct tcttcttttc ttttcttttc tttttctttt ttttcttttt
     2881 cttctttttt ctttttttct ttttttttct tttctttctt cttttttttc ttctttttct
     2941 ttcttttttt tctttttttc ttttttcttt tctttttctt ttcttttctt tttcttcttc
     3001 tttctttctt ttcttctttt tttcttcttt ttctttcttt ttcttttctt tcttttcttt
     3061 ttcttttttc tttttctttt cttttttctt tttcttcttt tcttcttttt tctttcttct
     3121 ttttttcttt tctttctttt cttttttttt cttctttctt tttcttcttt ttttttcttt
     3181 ttttcttctt ttcttttctt cttttctttc tttttttctt tcttttcttt tctttctttt
     3241 tttcttcttt ttcttttttt ttctttttct tcttttttct tttttctttt ttcttctttt
     3301 tttttctttt tttctttttt cttttctttt cttctttttt ctttttttct ttttttttct
     3361 tttctttttc tttctttttt tcttttcttt cttctttctt tcttcttttt ttttcttttc
     3421 tttttctttt ttcttttctt tctttctttt tcttcttctt tcttcttttc tttttcttct
     3481 tctttttctt tcttcttctt ctttttcttt ctttttcttt tttctttctt ttttcttctt
     3541 tttcttttct tttctttctt cttcttttct tttttctttc tttttctttt tctttcttct
     3601 ttttcttttc ttctttttct ttttttcttt ctttttcttt ttcttttctt cttcttttct
     3661 tttctctttt ttcttcttct ttttttcttt tctttctttc ttttctttct tctttttctt
     3721 ttcttttttc ttttctttct tcttttcttt cttttctttc tttcttcttt tcttcttttt
     3781 cttcttttct ttctttttct tttctttctt ttctttcttt ctttcttctt ctttcttttt
     3841 cttcttttct ttcttttttc tttcttcttc tttcttcttt ttcttttctt ttctttcttt
     3901 ttcttttttt cttctttctt tctttttttc ttttcttctt tcttttcttc tttctttttc
     3961 tttcttcttt tcttttcttc ttctttttct tttctttttc ttttcttctt ttttcttctt
     4021 cttttctttc tttttctttt tcttcttctt ttcttctttc tttctttttt tcttctttct
     4081 ttctttcttt tttctttttc tttcttttct tctttctttc tttttttctt ttcttttctt
     4141 ttcttctttt ctttttcttt cttcttttct tttcttcttt tctttctttt ttttcttctt
     4201 tttctttttc tttttttctt tctttttctt ctttcttttc ttcttttctt tctttttctt
     4261 tttcttcttt ttcttctttc tttcttttct ttcttttttt cttttttttt ctttttcttt
     4321 ctttttcttt cttctttctt tttcttcttt tttctttttc ttcttttctt ctttctttct
     4381 tttctttctt ttcttctttc tattctttct tcttcttctt cttctttttc tttttttttt
     4441 cttttttttt tgtctttttt tttttttttt ttttttgttc ttttcttttc tttttttttt
     4501 tttttttttt tttattcttt ttttttttct attttttctt tttttctctt ttcttccgct
     4561 ttttttcttt tctccatttc ttctctctta ctcttttctt ttttcctttt ttcttttttt
     4621 ttttttctct ttttcttttt tttttttttt cttctatttt cttttttttt ttttttcttt
     4681 tttcttttgt ttttcttttt ttttgggcac tttttttttt cttttttttg tttttatttt
     4741 tttttttgct tttgtttgtt tttttttttt tgtttttttc agtttttttt tttctttttt
     4801 tatttttttt ttttaggttt ttcttttaat tctttttttt tttttttttt ttatttttat
     4861 ttcattttta ttccatatcg agtccatcat tgcaaattga tatgttatgt attttattca
     4921 gtcccattat atcgctatgt gtagtatctc ctcttataat tgtcgatatt ttccttacta
     4981 actctgaaaa attactaacg caccccgcac ttcgtagttc ttgttttgat aaaattttac
     5041 cattggaatt tatgcgtcga aacacttcgt caatgtggtc ctctgttgaa gagtattcag
     5101 atatagcaaa tctatagcga gcaattaatg tagaactttc gcgggatagc actggctctt
     5161 tttgttgaag tttaccactg tcttttagtt gttttgttaa tgcaatggca tctaaattaa
     5221 agtatttacc atttaatgaa aatcgctgct caataaaact cattaaagct tctagtcttt
     5281 gcattccatc aaggatttct cttttgttgt caatggaaac aaacaatagc aaagggatag
     5341 ggtattgctg agtcattgat gaaatcagtg ctgttttctc ttcgagggac catactaatt
     5401 ttctttggta tcttcgattt ataattaatt tatcatccag ataccactcg tataattctt
     5461 ctgctgtctt ggagtcaata tctaagtttt cattagccat tagatgtatc tcgagtattt
     5521 agttaagaaa ctgataggtc agtatttgca tcaatgttca ttgaatttgt atccgaagtc
     5581 aatctgatgt aggaacttca aaactgcgca aaacacgcaa atacgcacaa gaaagtaggg
     5641 ctgcgcttgg aattcggaaa ataaaccaca tcaaaactac tttttagcca aataacgttt
     5701 gggaatcacc agaatggtgg gacaacagcg gttttagtgc cctaaatcgt acgttttcat
     5761 ccagttgccc ctcaaacccc atgttcaagt cagaatagtg gacaggcggc caagaacttc
     5821 gttcatgata gtctccggaa cccgttcgag tcgttttccg ccccgtgctt tcatatcaat
     5881 tgtccggggt tgatcgcaac gtacaacacc tgtggtacgt atgccaacac catccaacga
     5941 caccgcaaag ccggcagtgc gggcaaaatt gcctccgctg gttacgggca caacaacagg
     6001 caggcgggtc acgcgattaa aggccgccgg tgtgacaatc agcaccggcc gcgttccctg
     6061 ctgctcatga cctgcggtag gatcaagcga gacaagccag atttcccctc tttccatgtc
     6121 agatttcctc ctgaccagtc gccggtgcat ccagccattc tcgttcttca gctgatattt
     6181 cagcattcgg atcacactgt gccagtagct cagccagtga atattgcggg cgtctgtacg
     6241 gctcaacaat cagccggcca ttatcaatga ccatgccaac ttcattatct gtgcccagag
     6301 acagcgcatt cagcagtgcc ggtgggacgg tcagcataac tgagccgcca accctcttca
     6361 gtcgggtggt atgcattctt cacctccata aaagttatat ttaaatataa catccactaa
     6421 aaaacacacc aggctttaac gcacaatgtt taataaaaat ataactttca accaaacagt
     6481 a
//
