LOCUS       CP088478                6989 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain E17EC0134 plasmid pE17EC0134-4, complete
            sequence.
ACCESSION   CP088478
VERSION     CP088478.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391666
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:57:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,011
            CDSs (total)                      :: 4,893
            Genes (coding)                    :: 4,738
            CDSs (with protein)               :: 4,738
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 74 of 155
            Pseudo Genes (incomplete)         :: 99 of 155
            Pseudo Genes (internal stop)      :: 33 of 155
            Pseudo Genes (multiple problems)  :: 42 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E17EC0134"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pE17EC0134-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            110..670
                     /locus_tag="LQT86_25005"
     CDS             110..670
                     /locus_tag="LQT86_25005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UGE93478.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEDTLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            677..910
                     /locus_tag="LQT86_25010"
     CDS             677..910
                     /locus_tag="LQT86_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UGE93479.1"
                     /translation="MTELEMHLLNALEQLQQDYMQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1394..1816)
                     /locus_tag="LQT86_25015"
     CDS             complement(1394..1816)
                     /locus_tag="LQT86_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UGE93480.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1938..2216
                     /locus_tag="LQT86_25020"
     CDS             1938..2216
                     /locus_tag="LQT86_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE93481.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(2208..2498)
                     /locus_tag="LQT86_25025"
     CDS             complement(2208..2498)
                     /locus_tag="LQT86_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE93482.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            2739..4385
                     /gene="cka"
                     /locus_tag="LQT86_25030"
     CDS             2739..4385
                     /gene="cka"
                     /locus_tag="LQT86_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="UGE93483.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVSNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(4382..4672)
                     /gene="cki"
                     /locus_tag="LQT86_25035"
     CDS             complement(4382..4672)
                     /gene="cki"
                     /locus_tag="LQT86_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="UGE93473.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            4702..4833
                     /locus_tag="LQT86_25040"
     CDS             4702..4833
                     /locus_tag="LQT86_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UGE93474.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5028..5171)
                     /locus_tag="LQT86_25045"
     CDS             complement(5028..5171)
                     /locus_tag="LQT86_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGE93475.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(5839..6030)
                     /locus_tag="LQT86_25050"
     CDS             complement(5839..6030)
                     /locus_tag="LQT86_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UGE93476.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6098..6421
                     /locus_tag="LQT86_25055"
     CDS             6098..6421
                     /locus_tag="LQT86_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UGE93477.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5667..5754
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(5100..5656)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6412..6989
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="5.83e-122"
ORIGIN
        1 actgaggcgc ttacagcggc aggttttgag gttgtgagga caaccaggag cagtatcagt
       61 atcgccgacc cggagggtgg gcgtaatctc aggctgaaag gggcgctgta tgagcaatct
      121 tttacaaatg gggacagact tcgagaagaa actgaaagag cgtgccgtct ctacagagaa
      181 catgctgaac agcgagttca ggaagctcga agcgtctgta gacagggaac tgaacttaaa
      241 cgggcagaaa atcagagacg ctatcagcgc acacacagca gcggtgaaag aacaactgga
      301 gacgctgaac agtgccgtga acgcgcagtt cagcgccaca gagaacgaac tggcccggcg
      361 gcaggaagag ctgctctgga agctggtcaa agggcggata ctgtacccgt cgctgacggc
      421 gctttgcgtc acgggcggga tttttctggc gtcatggggg ctgattcagt ggcaggaaag
      481 ccggattgcg gcgaacatcc tggctatcag ggaccaggaa gatacgctgg cgaaactccg
      541 ggaaaaaaca tggggtgtga cgtatcacga gggcaggaac gggaaatttc tagtgttgcc
      601 gtccggggtg aagggggaaa acaactggac ggtggagaag aagaacgcag taagactggt
      661 cagggagtaa gtgagtatga cagagctgga aatgcactta ctgaacgcct tagagcaatt
      721 gcagcaggac tatatgcagc ggctgaaaga ctgggagagc gccttcacgg atttgcagca
      781 tatgttcgag ctgacacgac aggagaacgg gcaactgagg gaacagtgcg acgacttgag
      841 tcagcaggtg cagagattag ccgggcaggt ggaccacttg agtcggttat ttatcacgaa
      901 cagcagataa gggatgaaca ggaaaggcag gcacgcctgg aacgggagcg tgcgcggtcc
      961 tggcatgaag agcaggaacg ggaagcacgg gtatggcgtg gcccgtcaat aggtatgtga
     1021 cggtgagttg ttccgggagg ttgccagatg gtaccggaac gcgttaaaaa cgcatccggg
     1081 cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct ctttgccaga tcgcgctcat
     1141 tcctcagcct catcccgcga tgtctgcgta tgtcatcaac tgaaaagatg gaaaaaaaac
     1201 agtgtcatga ggtttacgca ggcaaaacca gcgttattca catggctgaa tagctatgca
     1261 tacggttaaa atttatcagg tgcgatcgcg gcagcttttc gggtggtttg ttgccatttt
     1321 tacctgtcag cagccgtgat cgcgctgaac gcatttcagc ggtgcgtaca attaagggat
     1381 tatggtaaat cccttactgt ctgccctcgt atccatcgag ataaaccgca gaaatggtgt
     1441 ctgccattag tttcattttt ttcggcgaag gcatatcgct gtttgtgtat tttctgccag
     1501 caaaggctgt tgtgctccgg agtgagtgaa aaaatttttc tgagtggaat tcttttacac
     1561 gttgctgtgc cgtttctgct gatactccgg ctgctttgtc tttcatcccc atatcccgga
     1621 actgctgaaa gaacgggagc atcatacgta tggtgtctgc tttctgggtg tagaagttag
     1681 gatcaaatcc atcatctgaa gcataaacat aatgccgggt atgtcgttcc aggctggtct
     1741 ttggtgtgca tcctgtaaga gctattgttg ctgttatcag aggtatcaga agcagcctgg
     1801 cagcggcaga tctcataaac atctccttgc gtttttaatc ttgatactca gggagttata
     1861 tctccgaaga gtatctctta tcatacgtcg gtttactccc aatgtgattt gatggtcaat
     1921 gagtcaggaa taatcccgtg aaaaaatgtg tgccagtcct gctttttagt gttctttctg
     1981 cttcggttgc cagtcatgcg gacagcaatg tttttcaaca aaaggccgag aagacaaaaa
     2041 acggcattgt tgcggatgtt aatgccagac agaaaatgtt cagtaataga ctgaagatac
     2101 aacttcagat cgatgagttg agagcagagc tggctggcac cagagatgcc caaaaaagag
     2161 aggatttgcg taaaaaactg gagtctctgg agcggcaaaa gaaatcctta cgctgatggg
     2221 ggcgtatttt ccccggtttc ttccataacc tttttaccgt tcagatattt acgggcgagg
     2281 cgaatgcgtc ttgcctggta taccgccgag taagcctggg tgggattcac accattactg
     2341 atttcactga ccacatgacg ctgatcagcc agtgtcagcg tgtaaacctg tttctggatg
     2401 aggtcacggg ctttgtcatg ctcatatccg gcagcaacaa gggtgtcgag aagtcgtttg
     2461 tctgttttgt tcagtttatg gcggggtgat ttgctcacat cgttctccgt tttttcatga
     2521 tccaacagtt ttatatctca tcctgatttg ctccggtgtt ctgtttcgac gttctcaatg
     2581 cctgcctccc catagtgtga tccttattgc aaaaatgaaa atccatgctc ttgacatgga
     2641 caatgctgag tagtaggttt ttactgtata taaaaccagt ggttatatgt acagtatatt
     2701 gtttttaatt tattgtttta attatcaaag aggaatttat ggctaaagaa ctaagtggat
     2761 atggaccaac tgctggtgag tctatggggg gaacaggggc gaatctgaat cagcaaggtg
     2821 gaaataataa tagtaatagt ggggttcact ggggtggtgg ctctgggcat ggtaataatg
     2881 gtggtcaggg aaattcaaat tcatctggtt ctacatcaac tgtgatgaaa accggtgagt
     2941 catatctgac tccgtggggt gatgttgtca ttaataatga tggccttccg gtgatgaatg
     3001 gtattgttat gacagaagag aattcaactc tggttgataa tccatttgga ggggtctcca
     3061 gagttcttaa ttctttaatt agtgatatgc catcactttt tgcagaaagc tcaggaaata
     3121 acaacaataa cactgcttca gttaatactg caccaacgaa tgctcaggtc agtgatatgg
     3181 ataagagttc gaaggtggtg agtaatgtaa ttaatgaaaa acaaaagcag aagaataaaa
     3241 ttgctacaca gattagtgaa aaacagaaga aaatcgagga gatgaaaaaa gtattcaagc
     3301 atcattctta tcatggtatc actgatcttg agagggatgt tgatgagttg caaaaaaaat
     3361 caaatcagct tgatgcagat attagcaaac ttaattctta taaaaataca ttgcaaagta
     3421 aaattggtga tgtgaataaa cagaaggaag ctgaagaaaa ggctcgtgaa aatgcagagg
     3481 tcgctgaaca tgaaactctt aatgaagaga aacaggcggt ggcggaagca gagaagcgtt
     3541 tagctgaggc gaaagctgaa ctggcgaagg ctgaaagtga tgtacagagt aagcaagcta
     3601 ctgtttccag agttgcagga gagcttgaaa acgctcagaa gagtgttgac gtgaaggtca
     3661 ccggatttcc gggatggcgt gacgttcaga aaaaactaca gcgtcagctt gaagctaagc
     3721 aggctgagta ctccgcagtt gaaaatgaac ttaagaatgc tgttagtttc agggatggta
     3781 aggccgctga ggttaaagag gcagagcaga aattaaaaga agctcaggat gctcttgaaa
     3841 agagccagat aaaagatgct gttgatacta tggttggatt ttatcaatat ataaccgaac
     3901 aatatggaga aaaatacgca aaaattgctc aggatttagc ggaaaagtct aaaggaaaga
     3961 aaattcaagg tgttgatgaa gctctggcag catttgaaaa gtataagaat gtactggata
     4021 agaaattcag taaggttgat cgggatgcta tttttaatgc gttagagtct gttaattatg
     4081 atgagttatc taagaattta acgaagatat caaagtcgct taaaatcacc agtcgagtat
     4141 cttttctata tgatgttggt tctgatttta aaaatgcaat agaaactggt aactggcgac
     4201 ctttgtttgt cacgctggag aaaagtgcgg tcgatgtagg tgtggcaaaa attgtggcat
     4261 taatgtttag ttttattgtt ggtgtgcctc ttggtttctg ggggattgca attgtcacag
     4321 gtattgtttc ttcttacata ggggatgatg agttgagcaa acttaatgag ttgttaggga
     4381 tttaatttct ctttagagtg taatatatga aacttggcaa tcctactggg attgccatta
     4441 ataaataaaa caatgcagat aacttgcata agtaccaggg ttcttttttt aactttgtat
     4501 tttctttgat atatctagat atggttaatt tagagtatgg atatagcaat acatgtattg
     4561 atgatataaa tataaataac aaaggagtgt tatcattgtc gttaaatatt aaaataagaa
     4621 tccatggtat aagtgattca ggtaaattat gtaggtagta ttttaaatgc ataagtattt
     4681 ttacttttgt tggaaagaaa tatgaaaata ataatttttc taattacaat attattcttg
     4741 tcaggatgtc aagcaaacta tattcgtgat gtacaaggag gaaccgttgc accatcttcg
     4801 tcttctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc
     4861 ctttttttct gcgcgtaatc tgctgcctgt aaacgaaaaa accgcctggg gaggcggttt
     4921 agtcgaaggt taagtcagtt ggggactgct taaccgggta actggcttta gtggagcaca
     4981 gataccaaat gctgtccttc cagtgtagcc gctataaggc ttcaacttca agagttctgc
     5041 gtatctaaat ccactatcca gttaccaatg gctgctgcca gtggcgttaa gtcgtgtctt
     5101 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5161 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag ataccaacag
     5221 cgtgagctat gagaaagcgc cacgcttccc aaagggagaa aggcggacag gtatccggta
     5281 agcggcaggg tcggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat
     5341 ctttaagtcc tgtcgggttt cgccacctct gacttgagcg tcggtttttg tgatgctcgt
     5401 caggggggcg gagcctatgg aaaaacgcca gctgcgtggc cttttccggg ttcttgtttt
     5461 ttgctcacat gtttcttttg gtgttatccc ctgattctgt ggataaccgc attaccgtct
     5521 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg
     5581 aggaagcgga aaagcgcctg gacgagcatt ttctccttac gcatctgtgc ggcatttcac
     5641 acccggcatg gtgcactttt catacaatcc tcactgatgc caaatgatta agctagtata
     5701 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc taaaacccgc
     5761 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacaggccag ctgtgaccgt
     5821 ctccgggagc tgcatgtgtc agaggttttc accgttatcc ccgaagcgtg cgaggcagct
     5881 gcggtaaagc tcgtcagcgt gatcgtgaag cgcttcgcag atgtcagcct ggtcgtcggc
     5941 gtccagttca ttcagttttt ccagcagggt cagcgtctgg cttctgatga agcgggccat
     6001 gttaagggcg gttttttcct gtttagtcac agttctgttt ctccatgtgt ggggagtctg
     6061 tcggaggttt ttaatgatac cggtgaaacg cgaaaggatg ctcacgatac gggtcacgga
     6121 tgaggaacat gcgcggctgc tggcacgttg tgagggaaaa caacttgcgt catggatgcg
     6181 taaggtctgc ctgggggcac cgccatcgaa aacgtcaggt cttccgacac tggcaccgcc
     6241 gctgttgcgc cagttcgctt cggtcggcaa taacctgaat cagatagccc ggaagataaa
     6301 cagcggacag tggtcggggc atgaccgggt tcatgtggtg gctgcactga tggccatcgg
     6361 acgtgaactg agtgagctga gggatgaggt ccgtaaacag ggagaacggg atgatagtta
     6421 agtttcatgc caggggaaaa ggcggtggca gtggtccggt tgattacctg ctgggccggg
     6481 agcgtaaccg ggaaggggcg agagttctgc ggggtgctcc cgaagaggtg cgggaactca
     6541 tcgatgccac gccgtttgcg aaaaaataca cctcgggggt tctgtctttt gcagagcaga
     6601 ccctgccacc gggagagcgt gaaagggtga tggagagctt tgagcgggta ctgatgcccg
     6661 gtcttgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag gggcgtcttg
     6721 agctgaattt tgtcatcccg aatatggagc tggcgagcgg aaaacggctc cagccctatt
     6781 acgaccgagc ggaccgtccg cgaatcaatg cctggcagac actggtgaat caccattacg
     6841 ggctgcacga cccgaacgca ccggagaaca ggcggacact tgtcaccccg aataatctgc
     6901 cgaaagcgaa acaggaggcc gcagaagcga ttacgagggg actggaggct ctttaccatg
     6961 ccggagcgct aaaaacgcgt caggatgtc
//
