LOCUS       CP088477               16235 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain E17EC0134 plasmid pE17EC0134-3, complete
            sequence.
ACCESSION   CP088477
VERSION     CP088477.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391666
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16235)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16235)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:57:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,011
            CDSs (total)                      :: 4,893
            Genes (coding)                    :: 4,738
            CDSs (with protein)               :: 4,738
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 74 of 155
            Pseudo Genes (incomplete)         :: 99 of 155
            Pseudo Genes (internal stop)      :: 33 of 155
            Pseudo Genes (multiple problems)  :: 42 of 155
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..16235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E17EC0134"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pE17EC0134-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="34.87 N 126.99 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(15705..16235,1..5)
                     /gene="traP"
                     /locus_tag="LQT86_24925"
                     /pseudo=""
     CDS             join(15705..16235,1..5)
                     /gene="traP"
                     /locus_tag="LQT86_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /name="traP"
     gene            complement(420..818)
                     /locus_tag="LQT86_24930"
     CDS             complement(420..818)
                     /locus_tag="LQT86_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE93470.1"
                     /translation="MLTSKEAKNNANNHSLQACQSGTWRKSNSGSTVITGRKVGSRITL
                     QDQLQLMMQIELLNVVFYDEYNFHKGTFRADLTGNVVMSSRVRTEIKLLALLPTCQSGT
                     WEIIISVDMDEYKNIYVISQKHTGLREV"
                     /name="hypothetical protein"
     gene            817..1972
                     /locus_tag="LQT86_24935"
                     /pseudo=""
     CDS             817..1972
                     /locus_tag="LQT86_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /name="tyrosine-type recombinase/integrase"
     gene            2123..2943
                     /locus_tag="LQT86_24940"
                     /pseudo=""
     CDS             2123..2943
                     /locus_tag="LQT86_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /name="conjugal transfer protein TraE"
     gene            3028..4228
                     /locus_tag="LQT86_24945"
                     /pseudo=""
     CDS             3028..4228
                     /locus_tag="LQT86_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /name="conjugal transfer protein TraF"
     gene            4287..4871
                     /locus_tag="LQT86_24950"
     CDS             4287..4871
                     /locus_tag="LQT86_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="UGE93471.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEINA
                     DNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNAVR
                     TIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLARDRL
                     GKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
                     /name="histidine phosphatase family protein"
     gene            5266..5720
                     /gene="traH"
                     /locus_tag="LQT86_24955"
                     /pseudo=""
     CDS             5266..5720
                     /gene="traH"
                     /locus_tag="LQT86_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /name="traH"
     gene            5717..6542
                     /gene="traI"
                     /locus_tag="LQT86_24960"
                     /pseudo=""
     CDS             5717..6542
                     /gene="traI"
                     /locus_tag="LQT86_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /name="traI"
     gene            6529..7674
                     /gene="traJ"
                     /locus_tag="LQT86_24965"
                     /pseudo=""
     CDS             6529..7674
                     /gene="traJ"
                     /locus_tag="LQT86_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /name="traJ"
     gene            7671..7961
                     /locus_tag="LQT86_24970"
     CDS             7671..7961
                     /locus_tag="LQT86_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="UGE93472.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTGI
                     ILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
                     /name="conjugal transfer protein"
     gene            8020..8522
                     /locus_tag="LQT86_24975"
                     /pseudo=""
     CDS             8020..8522
                     /locus_tag="LQT86_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /name="phospholipase D family protein"
     gene            8611..12373
                     /locus_tag="LQT86_24980"
                     /pseudo=""
     CDS             8611..12373
                     /locus_tag="LQT86_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /name="DUF5710 domain-containing protein"
     gene            12391..12738
                     /locus_tag="LQT86_24985"
     CDS             12391..12738
                     /locus_tag="LQT86_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="UGE93469.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARAA
                     ADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEKLP
                     GNIDPWKDYNL"
                     /name="conjugal transfer protein"
     gene            12813..13426
                     /locus_tag="LQT86_24990"
                     /pseudo=""
     CDS             12813..13426
                     /locus_tag="LQT86_24990"
                     /inference="COORDINATES: protein motif:HMM:NF022835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /name="DotI/IcmL family type IV secretion protein"
     gene            13437..14417
                     /gene="traN"
                     /locus_tag="LQT86_24995"
                     /pseudo=""
     CDS             13437..14417
                     /gene="traN"
                     /locus_tag="LQT86_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /name="traN"
     gene            14420..15689
                     /gene="traO"
                     /locus_tag="LQT86_25000"
                     /pseudo=""
     CDS             14420..15689
                     /gene="traO"
                     /locus_tag="LQT86_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /name="traO"
     Typing          5798..6171
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.2"
                     /coverage="99.0"
                     /evalue="4.56e-144"
     Typing          12392..12735
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="91.379"
                     /coverage="100.0"
                     /evalue="1.6599999999999991e-54"
     Typing          12736..13073
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.345"
                     /coverage="91.0"
                     /evalue="1.95e-118"
     Typing          13619..14190
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.927"
                     /coverage="100.0"
                     /evalue="2.16e-174"
ORIGIN
        1 ccaatccggt acctggagaa aggtaggatc tggtgagctt caaattgcta cagcacaggc
       61 cacaggttgg cgctttcctg gagcaacagc tacttgtcca actggaaaac gtgttacagg
      121 tggcggcgga atatgcacct cacgaactgg ttacatatgg cttaccaggt cattcccatc
      181 agccaataac tcatggtcgg ctgcgtgcga tacaaccgaa gatcagaatg gttcaataac
      241 tgtatatgcc atttgccaat aagctatttc aagcaagtaa tcttcataaa gtaggacgaa
      301 tactgtgtgt agtttttggc ttgccaagtc atctcgccgt tagagtcagg catgtcaata
      361 ggaacgactt ctgtttctgc catttcacgc cgtcaatacg ataggtgtta atacacagtt
      421 taaacctccc taagacctgt gtgtttttgg gatataacgt aaatgttttt atattcgtcc
      481 atatcgacgc tgatgatgat ttcccaggta ccggattggc acgtaggcag cagcgccagc
      541 aatttaatct cagttctgac acgcgacgac ataactacat ttccggttaa atcagctcta
      601 aatgtccctt tatggaaatt gtattcatca taaaaaacca catttaacaa ttcgatttgc
      661 atcataagtt gcaactgatc ctgcaaagta attcggcttc caaccttacg accagttatc
      721 accgtactac cagaattgct tttacgccaa gtaccggatt ggcacgcttg cagagaatga
      781 ttgttggcat tattttttgc ctcttttgag gttagcatgc cgtctccacg catccgtaaa
      841 atgtccctgt cacgcgcact ggataagtac ctgaaaacag tttctgttca caagaaaggg
      901 catcaacagg agttttaccg gagcaatgtt atcaagcgat atcccattgc ccttcggaat
      961 atggacgaaa taacaaccgt tgatattgct acatacagag acgttcgttt agcagaaatc
     1021 aacccccgaa cgggtaaacc cattacaggt aatactgtac gtcttgaact cgcccttctg
     1081 tcatctctgt tcaatattgc tcgtgttgaa tggggaacct gtcgtactaa cccggttgaa
     1141 ctggtttcgc aagccgaaag tatcctcggg acgagatcgc cggctaacgt cttcagaaga
     1201 acgtcgcctt tctcgctatt tccgcgaaaa aaatctgatg ttgtatgtca ttttccatct
     1261 tgcccttgag acagccatgc ggcagggcga aatactggcc ttacgttggg agcacattga
     1321 tttgcgccac ggtgtggctc atttacctga aaccaaaaac ggtcactcac gggatgttcc
     1381 tctgtccaga cgtgcccgta actttcttca gatgatgccc gttaatctcc acggcaatgt
     1441 ttttgattac accgcatccg gctttaaaaa tgcctggaga atagccacac aacgacttcg
     1501 catcgaggac ctgcattttc acgatctacg gcatgaagca ataagccgct tcttcgaact
     1561 gggtagcctg aatgtaatgg agattgctgc aatatcagga catcgttcca tgaatatgct
     1621 gaaacggtat actcatcttc gtgcatggca actggtcagt aagcttgatg cccgccggcg
     1681 gcagacacaa aaagtggcag catggtttgt gccgtatcct gcccatatca cgaccatcga
     1741 tgaagaaaat gggcagaaag cgcatcgtat tgagatcggt gattttgata accttcacgt
     1801 cactgccaca acgaaagagg aagcagttca ccgcgccagt gaggttttgt tgcgtacact
     1861 ggccattgca gcacagaaag gcgaacgtgt cccatctccc ggagcgttac ctgttaacga
     1921 ccctgactac attatgattt gccctctgaa cccgggcagc accccgctgt aaacatcatt
     1981 caaaacacat acagaaaggc gagttgcaac aactcgccaa acacattcga tctgcatgat
     2041 cgaaattatt cgtttcacca gaaaaaacac cgaagataca ataaccacat gagctgaaaa
     2101 gactttttca gtcggaggaa caatgagact gaatacgacc ggtattgcgg cccgaatgat
     2161 gctctcgctg gacaaaaaag caatcggcga aaaactcatc aacggtcgtc agataaaccc
     2221 gacacctctg caggtccgtt acccccaagg ggtaagagaa gttctgggga taatgagcga
     2281 acaattagcc atatccaccg ctgatttaac ccgaatcctg cttgaggatg ctcttcataa
     2341 tatgtttatg cctgcggata acaccacagg aaacataata agcagaattg aatacatcat
     2401 gctgtcgcat gatataaatg cgccattact ggcaacattg ctgtcgccat ggaatatccg
     2461 ctccacggtt atacaggacc cggccagact tgcagactat ctttcagctg acgctctcga
     2521 acatcttgct gaatgtttta atctcaatcc ggactggctg aatggccatg aaaactatcc
     2581 gatagcgttg tccggtgaat ggccagatac tgctgataat ttcagaatgc ttattagtga
     2641 tagcagcaac actgaagtta ttttctggca tagtttcccc ttcgcaggaa acacgaaaag
     2701 agaatattgc gggtaatatt gagacagaaa aaagaaataa atggctctgt aatatatccg
     2761 gcactatcat tatcaccaac acttctgaac gatgaaaaaa gaaaatggtt aactgcatat
     2821 acaaccaggc agaatacaac aatgtctttg cgacgcgtca cgttgagacc tggccttgcc
     2881 gggaacctta ttacagggca aatactacct gttagcttat ttacgtcttt attaccctgg
     2941 taaataccag atagtaccaa agtaaaaatt acggcacatc cgtgtcatag cgttgttttg
     3001 tctaaattct gataaacagg aaattaaatg atgaagaaaa atcatattac ccgcaccatc
     3061 attgcatctg ctgtattatt ttcctttaac gctgcagcag cgacgtcata ttttgaagcg
     3121 cgtaacgatg caatgggtgg taccggggta gcatcttccc attatggtgt ggcacctctg
     3181 gcaaacccgg ctttactgac aaagcacaac agcaatgatg atttcagcct tcttctgccg
     3241 tctgttgggg cacaagttgc tgatcctgat gatgtatcaa ataaagctga tgatgttaaa
     3301 gatgactggg atttgtttga cagtgctgta gataaccagc atggcgtaca acaggcagcg
     3361 gcaaatctta aacatcgtct gcaggaattc cgcaatatca atgcagatgc tcaggtaggt
     3421 gtatcagctg tagcagccat ggcaaatgac actctgccgt ttgcccttat ggtcaagtct
     3481 tacggaactg tcagcgtaaa cggaaaagta aacgatgcag acctggatta tctggacaag
     3541 ttgccaatgg aacaattacc gacgtcgata agaatgccct gacctcacgc gcattcgggc
     3601 gtgcagctgt aattaccgac gtcggaattt catttgccaa agaactggaa acggccggac
     3661 aaaaatggtc tcttggcgtc acaccaaaat atcagcgtgt ggacctgttt aactacaacg
     3721 taacggttcg tgattacgac aaagacgatt ttgacggtga caagtaccac aacacgaaaa
     3781 acggattcaa cgccgatatt ggtgcttata cagacctgaa tgacaactgg actgtcggtc
     3841 ttgttgccca gaacatcatt ccacgcagta ttgatactaa agtcgtcaat ggctttaaag
     3901 aaaccttcaa agttcgtccg caggcaaccg ccggtgtatc ctggcataat gacctgttta
     3961 ccactgctct tgacattgat ctgactcctg ccagtggctt cacttctgac agcaaacgtc
     4021 agtttgcaag tgttggtgct gaatttaacg catggaaatg ggcgcaattg cgtgccggtt
     4081 atcgtcagaa catggcatca aactccggaa gcgccttcac cgctggcgtt ggtatctctc
     4141 cattgacgtc gtacatatcg atgtttccgg cctggtagga actgaccacg attacggtgc
     4201 aatggcacaa cttcagttca ctttctgatt actgtccccg cccccggcac taaaccgggg
     4261 gtatttttat attcagttta aataacatga tgaaaccacg ttcttcttat tcaaaaacag
     4321 cattcattct tttattttct gttttcctgg tagcggcagt gacaaaagca aaaagctcgc
     4381 ttcccgatat caccctggaa caggcaaaag aaattaatgc tgataacacc gttattttcc
     4441 ttttccgcca tggagaacgt tgcgaccgtt cagatatgcc atgctattcg gataaaagcg
     4501 gaatcactat taccggtaca gagaaagcac agcaggaagg tattaagttc gcaacaatct
     4561 tcagtgaata tgatatttac tccagcaatg cggtaagaac catacaaact gcaaaattct
     4621 tctccggtaa agaacccgtc gttatggaca gtttatcaga ctgcaataat gatctttata
     4681 agacgcttga atcaatcgca agagaatctc ataaaaggaa catagtaata atgacacata
     4741 atcattgttt atcatttctg gccagagata ggctcggcaa gaaattcaaa ccggcgtacc
     4801 ttgatgccct gatcatgcat tacgatggta cccggctcat acttgacgga aaatacaaca
     4861 aagaggcata gaggatattt gaatttacat aaattctgat atactcagta agatacaaga
     4921 gtgtacgggc acatgtgcct gtctgaattg caggttatcc ggacgagggc cgggaactgt
     4981 ttcagggaca agcgccggta cgttcgattc ataaatcgtc gagtcatcca tcatggatcg
     5041 attcggcgcg gagcgttaaa gattacgctc atattcgttt tatgtctgac gacacatcaa
     5101 ccagccagta tcaggaataa tcaccttcag taaactatga ggctatttac ctggccacac
     5161 tggcatttat ttccggtaac gcaagtatag ccggaaacaa acatcatcct gtctgtaccg
     5221 gagcaccggt atatcccatt ctgatttaac tgctcaggaa ttattatgaa ccgtattata
     5281 cctgcgggtg tcttgccgcc cttttctggc tggctgcaac tccccttctc agcatactcc
     5341 tgttccgcgt aactacggtg ctgcctctga tgtggcggtt acccggcaca ctcagtacac
     5401 attatgggac aaaggtgtat tttccggcca acttccggtt tcaacggggt ctcatcttct
     5461 gacagctaac agcgatcgcc tttcctttga ctgggaaggt gatgcaattg aattactgaa
     5521 cgaactggcc agagtcaggg gatgcagttt aactacaatg gtgtccgcct gccgttacca
     5581 gtcaaccttc acgtccggat atgacgtttt ccaatacgtt gagacttatt gaagcccaga
     5641 cagcctggag agcaacaatc catcaatatc cgggcttgtt acaggtcagc ttcatgcagc
     5701 cggagaaccg gaaaaaatga aagcactcac ctctctcctt ttgccagtaa tactgcttac
     5761 tggttgttct tcttccccta ccatctcgcc aggtgtgtca atacgccacc acctgacatt
     5821 aatgcatggc ttaaaccacg aagtgaaaag ccggatagtc tgtcagaaac acgctggaaa
     5881 atgctgacag acgcaggtaa aacgcttgga tttcgtggtg gcaaggctca gcgttcatgg
     5941 gagctgattc aggcgctcaa cgcccgggaa agcacactta atgccttata cgatttccgg
     6001 ccactgatta gcccggaagg ctggctacca ccggttattg atgaagcaca ggacgtagct
     6061 catatcacgc cagatcaaat ccggacctct tcccgggtct ggaccatcat tcgccctgaa
     6121 cgctttgtca gcaatccacc gggctggcgt gactggcttc tccggggctt tcaacaacag
     6181 ccactccggg tacagaaggc agcgtcgttc cagaagacag cgtgcaacga aaagtgtggg
     6241 aaaccgcatt acggcaggga tggcaggaag gaagacaaaa cgccgacctg acgcttgaag
     6301 ccaaccagaa aaccctcacc cgtgattacc ggggcatgat gctgtactca cttctctggc
     6361 gtcagggcat gatcacccgt ccggatgttt ctgatcaaat gcaaaccgtt acaggtgacg
     6421 ggaaaaaact ggtcactgga gatcgtgttc gtcgccttaa aaatcatgcg gaattcaatc
     6481 ttcagaaatc tcactggcgt ccgcttatag gcactgaagg aggctccgat gaatattcct
     6541 gatgagtttg gtctttttcc gtttcagcgt ttcactgcag atgaactacg gcatttcttt
     6601 gtctggtgtg ccgcacataa agtcagtgat gtggatctga ccggcgggtc acctgtatct
     6661 gtcagtcgct ttggccgacg ggtgcgttgt tccagtgcaa cgttaccaac cacactcatg
     6721 tcctccctga ttgatgaact gttcggtcgg gaagtcattc cccgcgttct ggcaggcaat
     6781 ccggttgaca ggacgataca gattaacggg gatgcatccg ggcgctatgg tcttaaacgc
     6841 ggagaacgca tccggttacg ctctcacctc attcagggca catcaggtgc ggaggagaaa
     6901 gcaatctcaa taaccatgcg tgtcatcccg acagaaatac cggacattct ctccatgaac
     6961 attgaaccag acctgcttga agcaatggtc tgcaaaagcg gtctggattt gtatgtggtg
     7021 aaaccgggtc aggaaaatca acgctgtgtt cagcactgta ccgttacatt atggacaatt
     7081 tccccgacgc caaatcgtga catatgaaga cccggtggaa tatatcctgg gaaatgaaaa
     7141 cgatctgctc caccgcatca ggcagaaatc gggcgtgatg tggtcagttt tgctgccgga
     7201 ctgcgttctg ctgtacgacg taatcccgaa attatcggtg ttggtgagat cagggataac
     7261 gaaaccgccg acgcagcggt acaggcaggc aacaccgggc attactgcct gagcaccatg
     7321 catacgaaat ctcctggtga aacgctggca cgtcttcttg ggctgtttcc ccctgtcatc
     7381 agggattcga tggcctgggc tgtcctgtca ctgttgcagt tcattctggt tcaggttctg
     7441 gtcagaacaa atgacggtgg tcgtaaagcc gtccgggaat atatcgtcat taacgatgag
     7501 cttcgcgaca atctttccgg aatgccgcat gcagagtggg ggcatcacat tgatgccata
     7561 atccggcagg agaagcgccg tatccgggac cagattctgg aaatgtatat ccggaatgaa
     7621 gttgaccgaa gagaagcgat tctttttatc ccgccagggg agttacggtc atgaaaagag
     7681 aaggtttctg gcgatatgca acaccgccag tcaacatcca gggcgtacct ctgccgttca
     7741 ccatgattta tctgtttata tgcccctttc cctccaaaac aaccttctgg atttgcaccg
     7801 gcatcatttt atttttcgtt attctcgacc gttcaggctg gactgtcagg actatctgta
     7861 cacgcatttt cagcattctg agaggggcca tcgcctccgg tcgtccatgg tggtatcgcc
     7921 accatacgga atcaccacga gactggactg gcctcgatta aattctggag tctttatgtc
     7981 aggtaaaaaa ctatcgttac tctgctccgg gtatcgctac tggcatgccc gttactgttc
     8041 acgacaccat cgtttgcaat gatcgacacg ccatcgtgaa ggtcggcttt tctcctgaag
     8101 gaagcgcatc cgctctggtg cttgacacaa tcaacagtgc ggaaagctcc atcaggatga
     8161 tggcatattc cttcaccgac ccgatgtaat gcatgcgctg gcaaaagcga aaaaacgcgg
     8221 agtggacgtc cgtattgttg ttgatgacaa ggggaatacg aaccggcaag tcaggaagcg
     8281 atgaaatata tcaacctgct ggacattcct ctccggactg tagatgcctt cccatccatc
     8341 acgacaaagt catcattgtt gacggaaaca cggttgaaac tggctcctat aacttctctc
     8401 gtgctgctgc acgcaagaat tcagagaatg tcgttgtgct caagaatatg ccggatgtag
     8461 ccgcacaata tcttgaacac tggcaggatc gctggaataa gggtacagac tggaaaccct
     8521 gacaaacatt tctcccccga ttttctatcc ccccagccac ccccggcttc cggcggggtt
     8581 tttttccgtc ttttttcagg agaagcactc atgccgtctg tatcccgcta ccgcacctgg
     8641 cttgccgtgc ctgcagatga aatagaagat ctcaaaaaag cacatccccc gatgaacggg
     8701 cacacgcccg tcatctggga caaggagcac aaactctggt tcgcccgccc gggggcggac
     8761 ctgtccaggc tggaccgctg gctgccccgg ccacaggacg tctccatgaa cggcagtgat
     8821 ccggtcaccg agtttgccca ggttctggag aatgccggac tggttctgaa agaactgccg
     8881 gtcatggacg ggaaaatcca ccgtgtcccg accgccgatg acaaaaaagg acaaaaaagt
     8941 ggcgcatatc gcggattcct ggacggcaga cctgccggct ggtacaggga ttatcgcagc
     9001 gcggatgatt cgccgatcac ctggaccttc tccggcggag aacagacaga tccccgcgca
     9061 agactccacc tgaaagcgca ttcaatgcaa cgcagggagg acgcggagcg ggaactgaaa
     9121 gcacagtaca accgacaggc cgcgtatgcc cgccgctatg tcaacaaatg gccgcaggcc
     9181 acagcacatg aatacctgac ccggaaaggc attcaggctc accgggggtg cgggtaaaca
     9241 acaaaaatga actggttatt cccttcagta accgtaacgg cgcgatacgc tcttaccagc
     9301 gtattccggt caccgggggg aaggatgccc gcatcctgaa agattcagag aaaaccggta
     9361 actggtttgc gctgggcaca ccgcgcaacg gtcagccggt actgtttgcc gagggctatg
     9421 ccactgcggc ctccctgcac gaagccacgg gccttccggt actgatgacg gtggatggag
     9481 gcaacatgat tgccgttgcc gaaaatgccc ggcaaaagtg gacgcaaagc cctttatctt
     9541 ctgtgccgac aacgatcatg caatccgggt taacaaaggt atcgtcagtg caacaaaagc
     9601 cgcagaactg acaggaggaa cggtcatctt tcctgcgttc accgatgccg aaaaagcgca
     9661 ggggctgacc gattcaatga cctggacgca agccggggac gggccgcttt tcagcatgtc
     9721 atcaacgccc agttagaaca catcggggtt tccaccccaa cagtaacacc ccggaaatcc
     9781 gcgaggcact ggtgatcggc aacctcgttt ttacaccggt ccacactgag gaaaaaacca
     9841 tgactccgac agaatacctg aaacatcacc cgatacaggg cacagccatg accagggacc
     9901 ttctctgccg tccgccacgc agcaggaaca accagctgct tccagcagta acattgctga
     9961 tgaaacacag tcatttacca gtcacgccac tgaaaataac ggcaaagatg agcggcacgc
    10021 tgataacgtt caggcgcaaa cggaaagctc tgtagttcag gactcccctg cagaatcacc
    10081 ggcgtccccg gaaagtacaa cggcatctgc ccctgatgaa cccgctcatc cggcggaacc
    10141 tccggaaaaa gttgtctcgg tggccacaga taaagactgg cgtgaatttg aagccgaact
    10201 gtcacaaccg gaaaaaaacg agtcacaaca ggaatccggc acttcacctg aaataccggc
    10261 ccctgctgcc gcccccgcat cccctgaaga cagtccctca tctgaaacca tgaatgaatc
    10321 agccgagcca tcaccttccc ctgatgcagt caaagaggaa atgaccgtga tgacggagcc
    10381 tgatgacaaa caaagcaccg gaatatcccc ggatactccc cgggaagagg cggtcaacca
    10441 tgtaactgaa gacattcccc ttcagccaga gccgttcctt cctgagtcag atggccccga
    10501 agaatacgaa aactacagcg catatcagga gctgatgaac aatgaaaatc ctgagcatct
    10561 gcaggaaaac agtgatatgc cgtcgcaacc ggacacagac cacgttcagg cagatgagtc
    10621 agaacttcag gccacaacag atactgcaga accggctgtc cgggatgaac ccactccaca
    10681 gcaacctgtt cagagcacat caccttctga taatacttca tcgtttctcg acaaagcgcg
    10741 tggctttttt acgcgtaaaa aaacggattc acaggcacat gagaacagtg atcccacgcc
    10801 ctcagcggaa accacgacga ctccaccgat acctgacagt attgtctacg ccccggaacg
    10861 ttctgatgca ccgatatcgc tcaacatgga cgaaattctt aaagagcttg actgggaaaa
    10921 acgagcagac agaaccgtcc tgtacaaact ggatggcaaa cctgccttca ttgaccgggt
    10981 aaaccggctg gaaatggtca acggtgccag caatgacgat cgcagcgttc tggccgcact
    11041 tgctgtggcc accaaatttt atgggggcgt catagagctg acgggcagtg atgcctttaa
    11101 gcaaaaagca atgcagttga ttatcgagca caacatcgat gtccggatga aattgccaga
    11161 tcaacgggct gaactagaaa aattacgcaa ggaaatggct gtcagtaagg atgcagttgt
    11221 cacccatcag ccaacaccgg aacttaaccg gaatacccca gaacaaccgg ctgtccctga
    11281 ccccgttcag gaaaaagaag ccacacaatc cccggcgtcc cctgttgccc ctgaagcatc
    11341 aaccgtcagc accgcctctg caccatcacc ggctgaacca ggcaaaacgg cagatgtggc
    11401 accagacgaa gaaccaccag gaaaacttcg cccgggagag agcgtgactg ccgtactgca
    11461 caattttggt cgtgctgaat atgcacccgg caaaggagaa agtttctttg ttgagcttaa
    11521 aaaccgcagt ggcagcaaac tctactgggg gaacaactgg aaagtctggt caaaaaccat
    11581 cagaaaggtg acgtggtgac actgacactt cagaaccgcg aacaattcat cctcccggga
    11641 gagcaaaaag cacgtttccg gaataagtgg tccatggagt cagttaccaa cgggatctcg
    11701 gtatcacatg ataatccgga taaaggtcag cgtattcagg cgattccggt cgaaaccttt
    11761 atgaaagtgg ctgcgcaaat cagccagggg tggcccgaag agatgaaagc gttacgtatg
    11821 ccagaaaacg tcggctcaca tctgttttat cggtgaagac cgacatccgg tctccgcgcc
    11881 acagaacgca aatcaggtaa cagaaataac cagcgcagcc cctgacaagc tgacacctgt
    11941 tctgggatca gtggataagg atacgcgtga actcaacctg ctacttgtcc agagtgccga
    12001 tgaacatctg cagggtgtgg tccggctgaa cggcacattg tatccggcac tggccacccc
    12061 atcggccgac aacagtcagc tggttatcaa tgcgctgacc gacaaaggtt tacgctttgc
    12121 aggttatggt gaagcggtaa accacgatgc tgacagcacg aatcgccccg caccggagct
    12181 tatgcagttt catctgaaaa cccgggaaga accgcttttt gctgctgtct acacgccgga
    12241 aaaacaacct gatgcgttgt accggaacct gggcttcgag cagtcatggc agcagtggtc
    12301 caacagccaa aagccggagg acaggcagga aaaaactctg catcaggatc tctcccacag
    12361 ccccggccga tgagccggta aggaagccat atgaacatgc ataagtctct ggttgccgtg
    12421 ctcggcctta tcctgatttc atccacggca agtgctgcaa cctgtcgtgc gggtagcgcg
    12481 gcacaggccg gtagtaatac aggatatgag cgagcgcgtg cagctgcgga tgcatggtca
    12541 caacgtgaaa acgatgtttc ctcatccctg cagtcctgtc tgtcacgaat caaaaaaatc
    12601 agcataaacc tcccccagtt ccccagcctt gacgacattc tgtcgcagct ggaaaaccag
    12661 gtctgcgacg cggtcgtgga caaggtgaat gaaaagctgc cggggaatat tgatccctgg
    12721 aaggactaca acctatgacc gaaaataccg agccatcctc gccatcacat ccggcacctg
    12781 acccggaaat actgaagagc acaatcaggg ctatgaagca gtcagaacag cgggcaaata
    12841 atgtccctgc gctgataaaa gcgcttttat gtacgggcac atgtcttctt atcagtatta
    12901 ctggtaacgc cattcagtac tggcactcaa cgaatgtgga gcgcgaatat ttcgctacag
    12961 acaatggtcg tctggtcagg ctggcaccaa caagtcagcc agcatggagt cagaatgatg
    13021 caatggcctt tggcagccag gcactggcga ccgcattcaa cctggatttg tgcactatcg
    13081 ttcacagatc tcctccctgt ctccgcgttt ttctgacgaa ggttttgtcg gatatgtgaa
    13141 tgcattacag gcatctaaca ttctggagac gatcaagaaa gagaagatga atctgaccgc
    13201 cacgacaggt gctggcgttc tggtacgaca gggacaaatg tccgacggcg tatggttctg
    13261 gacattccag tatcctgtcc gtatgcgtct ggtcggccag acaaccagca agccagaaca
    13321 gtcttttgtt ttcgaaatca ccatccagcg tgttgacccc agactgaaac catccggtat
    13381 ggaaatccgg cagatgatct cccgtaacgc cggaccgaac tcctgagata agtaatatgc
    13441 aatcacgtta tttactgagc acactgctcc tggtgtgcag cgccgccacg tctgccgata
    13501 acgcaggatg gcagaacgca cgcacaccac aaacgacgaa ttctgccagc gatcacgttc
    13561 agaacgcctc ccaaaacacc ggaggtgttc cggcaacgct ggtaaaaggt gagctgcctg
    13621 cccccggcag gccagcccct ggtacaggat gcggcaaggc ttgattctga gctctccgcc
    13681 gatgagattc gctcactacg cagtctcatg gcggataacg agcgggccat caatgcgccc
    13741 atcaccagtg ttgtaccccg tatcagctct ctgacagtta atctgtcccc cggtgccagc
    13801 cttcctcttg ttcgcacagc catgaacaac ctgagcgtgg tgacatttac cgatatcaac
    13861 ggttcaccat ggccacaatc cgatcctcct tataacgcgg caccaaaact ctttgatgtg
    13921 cagtacaacg agaacatggt gacaatcaca ccattgcggc catgggcatc cggaaatatt
    13981 tctgtctacc tgaaaggtct gtcggtaccg gtcatcctga acgtaaccag tggcgaaaca
    14041 gatacgccgt catccagtca ggaaatggac agtcgtctgg atctgcgaat accacggcag
    14101 ggaccaacca gcccggttgt ctccatcccg acggataaga tcgccctgca tgatgcaaca
    14161 ttacaggcat ttctggatgg tattcctccc gggacccttc agttaagagg ctgaaattca
    14221 caggtaatgt cccggataca acgatctggc aacacggcga tgaccttctg gttcgttccc
    14281 gggcgatgtt gcgcgatgaa tttgaacaga ccttgtcctc tgccgacgga actcatctct
    14341 ggaagcttcc cgtcacccca ctactgactt tctctgtgaa tggccagtcc gttcacgtga
    14401 caccagaact ggagtaatca tgagtgctga acaggatgcc ggtaaatccg gaaagaaact
    14461 ggctgccctg cttggactgg gcagcatcat cttatttggc ggcggctata tcgcgttttc
    14521 caaactatca gggagtaaca gcgatatgca gtcagccgtg aacattaaca gtgcagcaag
    14581 cggcggaacg cgcagcgtta ccgaaacgcc tcactatcgt gaactgttaa gagcggataa
    14641 tgaacgaggg gccgcagctg cagcccgcaa taatcagacg ttcattgcca gtcttccaca
    14701 ggggcttgat attccggaca ctccggaaaa gcaacaacag cctgcagcaa aaccggaaaa
    14761 ctatgctcat cgccaggctt ccggcacaca caggaggatc gagcagccag tgaaaaacgt
    14821 atggagcggc tgcaaaagct gattgtaaga ataaaagatc agcatcctgc cggtagtaca
    14881 ccaaccatag caacgactat gtggaataaa tctccggcag aaacaacagg gcagaatggt
    14941 acgcagcaat tcgcattaca gaatgccagc ctgtccacgc ctgtagcaga aaaagggata
    15001 caactgatac ctgctctgac ccgtataccg gcatatattg atactgcagt ggattctgat
    15061 aacccgtcat ccaaggttat cgcaaccatt cccgctggcc cctgggcagg ggcaacgctg
    15121 ttcagtccgg gagtcaaact ggtgggtaac ggcgtggaga tccatttcga ccggatgagc
    15181 tggaacggca tggaccttaa ggtcaatgca tatgctcaac gggaagacaa cctgatgtca
    15241 tccgtggcca gcaatgtgaa tacacgctgg tttaaacata tcattctgcc gtcagtgtta
    15301 ggaggtgtgg gcagtattgg cacactttat aagatgccaa cacgcaggtt attcagggta
    15361 actatggcac ggttaccgga cgcgtgggaa tgcccagtgg tgaagctgtt gcgggcgtta
    15421 ttgccggtgg tatggcggaa agaggaagtc agatattaac ccgccaggca gaaagtgagc
    15481 cctataaaca ggtagaagtg tatcagcatg aagtcgtatc aattctgttt gttgacccgg
    15541 taatgacaaa cgacgccagg tcatcttccc tttccagtgg catttcccct tcagttaatc
    15601 gtacatcaca ggcggaacaa cgctcgcagg cacgaatgca gacagccatg gagcaaagaa
    15661 aagctgtgat gcaacgtcgg tatgatgaac acggagacac cataatgaaa cctgaaacag
    15721 aaatagatga aaccggcagc ttcggtgaac ccgaagagaa acccgccccc ttctggaaac
    15781 gttccgtctg ggggatatct gtagcaacat gggggctatg tgcagtcgtg ttgctcgcag
    15841 ccatatggta tctgttcctg agagcgccat cggaaacagg catgcctccg ttaatgatgc
    15901 ggatgctggc gtacagacct ggcaaaccac acaagaatca tcaccttctg ttcagtccac
    15961 tgagactatg actgtccgga caggagaaga tatgagccag cttgcccggg atgtaaaaac
    16021 agagctggat aaccgcgatg aaaaaattca ggcaacactg aatatgcttc acgacagcat
    16081 taacaagctg ggcgaagcta taaaaaagga tgaagaatac gcacaggaaa cgcgccgtca
    16141 gctggatgat atccggagcc gcctgaacgg aataatgaca cagaaaagcg tcactgaatc
    16201 ctcttccaca ccgcatccag caaagaaaaa aacgt
//
