LOCUS       CP088460               17518 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0024 plasmid pF16EC0024-4, complete
            sequence.
ACCESSION   CP088460
VERSION     CP088460.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391676
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17518)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17518)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 180x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:41:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,213
            CDSs (total)                      :: 5,099
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 107 of 246
            Pseudo Genes (incomplete)         :: 155 of 246
            Pseudo Genes (internal stop)      :: 61 of 246
            Pseudo Genes (multiple problems)  :: 64 of 246
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..17518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0024"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0024-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            2..2716
                     /locus_tag="LQR18_25985"
                     /pseudo=""
     CDS             2..2716
                     /locus_tag="LQR18_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /name="AAA domain-containing protein"
     gene            complement(2824..4313)
                     /locus_tag="LQR18_25990"
                     /pseudo=""
     CDS             complement(2824..4313)
                     /locus_tag="LQR18_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /name="phosphoethanolamine transferase"
     gene            complement(4488..5198)
                     /gene="tia"
                     /locus_tag="LQR18_25995"
                     /pseudo=""
     CDS             complement(4488..5198)
                     /gene="tia"
                     /locus_tag="LQR18_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="invasion outer membrane protein Tia"
                     /name="tia"
     gene            complement(5589..6599)
                     /gene="papG-II"
                     /locus_tag="LQR18_26000"
     ARG             complement(5593..6599)
                     /name="papG-II"
                     /gene="papG-II"
                     /gene_name="P fimbria tip G-adhesin PapG-II"
                     /tool="amrfinderplus"
                     /drug_class="nan"
                     /identity="98.81"
                     /coverage="100.0"
     gene            complement(6643..7142)
                     /locus_tag="LQR18_26005"
                     /pseudo=""
     CDS             complement(6643..7142)
                     /locus_tag="LQR18_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /name="fimbrial protein"
     gene            complement(7217..7734)
                     /locus_tag="LQR18_26010"
                     /pseudo=""
     CDS             complement(7217..7734)
                     /locus_tag="LQR18_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /name="fimbrial protein"
     gene            complement(7761..7985)
                     /locus_tag="LQR18_26015"
     CDS             complement(7761..7985)
                     /locus_tag="LQR18_26015"
                     /inference="COORDINATES: protein motif:HMM:NF012635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="UGE64563.1"
                     /translation="MNAGRLGIALLDTDGSSLLKPGTSHNKGQGEKVTGNSLELPFGAY
                     VVATPEALRTKSVVPGDYEATATFELTYR"
                     /name="fimbrial protein"
     gene            complement(7997..8296)
                     /locus_tag="LQR18_26020"
     CDS             complement(7997..8296)
                     /locus_tag="LQR18_26020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE64564.1"
                     /translation="MIKSTGALLLFAALSAGQAIASDVAFRGNLLDRPCHVSGDSLNKH
                     VVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPAI"
                     /name="hypothetical protein"
     gene            complement(8306..8887)
                     /locus_tag="LQR18_26025"
     CDS             complement(8306..8887)
                     /locus_tag="LQR18_26025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein papJ"
                     /protein_id="UGE64565.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHHY
                     RINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSAAAGAPLMLRLAGCGDGGA
                     LLPGKRGVAMTVSGSLVTGHGTGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQEDML
                     AAWRKRAAQEGEYSDARSNPAMLRLSIKYE"
                     /name="protein papJ"
     gene            complement(8924..9641)
                     /locus_tag="LQR18_26030"
                     /pseudo=""
     CDS             complement(8924..9641)
                     /locus_tag="LQR18_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /name="fimbria/pilus periplasmic chaperone"
     gene            complement(9727..12233)
                     /gene="papC"
                     /locus_tag="LQR18_26035"
                     /pseudo=""
     CDS             complement(9727..12233)
                     /gene="papC"
                     /locus_tag="LQR18_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /name="papC"
     gene            complement(12293..12854)
                     /locus_tag="LQR18_26040"
                     /pseudo=""
     CDS             complement(12293..12854)
                     /locus_tag="LQR18_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /name="type 1 fimbrial protein"
     gene            complement(12944..13498)
                     /locus_tag="LQR18_26045"
                     /pseudo=""
     CDS             complement(12944..13498)
                     /locus_tag="LQR18_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /name="type 1 fimbrial protein"
     gene            complement(13702..14013)
                     /locus_tag="LQR18_26050"
                     /pseudo=""
     CDS             complement(13702..14013)
                     /locus_tag="LQR18_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /name="adhesin biosynthesis transcription regulatory family
                     protein"
     gene            14421..14642
                     /gene="papI"
                     /locus_tag="LQR18_26055"
     CDS             14421..14642
                     /gene="papI"
                     /locus_tag="LQR18_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="UGE64566.1"
                     /translation="MYENEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEKEGMV
                     QRSPLRRHGTYWFLKGEMQAGQNCSSTT"
                     /name="papI"
     gene            16073..16227
                     /locus_tag="LQR18_26060"
                     /pseudo=""
     CDS             16073..16227
                     /locus_tag="LQR18_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /name="transposase domain-containing protein"
     gene            complement(16551..16688)
                     /locus_tag="LQR18_26065"
     CDS             complement(16551..16688)
                     /locus_tag="LQR18_26065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE64561.1"
                     /translation="MTTGLPFWQTGWDNMKTVCAHLYNAMEKNGMRGVSIKYLCNTIDR
                     "
                     /name="hypothetical protein"
ORIGIN
        1 gcttggacac tccggagata agtttccgct ggcaaaggct cagcgcgatg ccttaagcca
       61 ttttctggat gcaagacatg gcgatatcct tgctgttaat ggacctccgg gaaccgaaaa
      121 accacgctgg tgctttctat catcgccacg cagtgggccg agcggctctc gaaaaatctg
      181 agcctccggt attatcgcga cttcaacgaa caaccagctg taacgaacat tatcgaagcg
      241 ttcgggaaag attttcacag ggcactggtg caatggccgg acgatggtta cctgagctga
      301 aaagcttcgg cgcttatttt ccctcaagct cgtaaaagct gaggcagcca aaaaatatca
      361 aactgaagat ttcttcaacc aggttgagtc aaaagagtat gtagaggatg cactgctgtt
      421 ttatctcgag aaagctaagg cagcttttcc tgaaaagagt gttcatcctg aaaaggtcat
      481 tgaactcctg catggtcagt ggtagcaaaa tccgagcatt gaaagactga acgcaacatg
      541 gcaaacgtta agccaggtac gggctgcgcg tgagcttatt gctaatgata ttgagcaata
      601 tctcgataat ttaaataaat tactttccgg acaagaacaa aaagtcactc tactgaagag
      661 tgctaaaacg gaatggaaaa aatatcgcgc cggtgaatca ctgatctatt cattattttc
      721 ttggctcccg gcggttcgta gtaagcgaca gtaccaaata cagctgtttc tcgaagataa
      781 attaggcgcg ctgattgcag gaaatcagtg gtctgatcct gaaactatcg aacgtaatat
      841 tgatgggctg ctcaattccg ctgagcgcga gcaaacaaca taccggcagc agattgactc
      901 cgcccatgaa atcgttctta aagaacagca gcggttcagg agtggcagag gctggcattg
      961 atttagggta tgagggcgac gaggaactga gcttctcaca ggccgatgaa ctggctgata
     1021 cgcagattcg cttccctgca tttttactgg acgactcact actgggaagg tcgttggctg
     1081 atggatatgg ccagaattga tgatctgcag gaagagaaga agaaaaaagg cgctaaaggg
     1141 gtaaccgccc gttggcaacg tcgaatgaaa ctcactccat gtgtggtgat gacatgctat
     1201 atgctgcccg gcaatatgca gataagtgag cacaaaggac aacgtaaatt cgagaaaagt
     1261 tatttgtatg attttgcccg atttactcat tgtcgatgaa gccgggcagg tgcttcctga
     1321 agtggctgct gcctcgtttg cattagctaa gaaggcatta gtgattggcg atacggagca
     1381 gatcccgcca atatggagta ttgctcccgc gattgatgtc ggtaaacatg cttgcggaaa
     1441 aaattctgtc tggcagtacg caagaagaga ttaccgagaa atatacggca atcgcagacc
     1501 ttggtaaaag tgccgcatct ggcagcgtta tgaaaatagc gcagtttgct tcgcgctatc
     1561 aatatgatcc cgaactggct cgtggtatgt acctatatga acaccgccgg tgcttcgata
     1621 atattattgg atactgtaat acgctctgct atcacggtaa gttgttgcct aaaagagggc
     1681 gtgaagagag caatttaatg ccgcaatggg ttatctccat attgatggta aaggagagca
     1741 ggcaagtagt ggaagtagat ataatttgct tgaggctgaa acgatagcgg cctggttggc
     1801 agagaaccag caaaatattg aagcgcatta cggcaaatcg cttcatgaag ttgtcggtat
     1861 cgtgacgcct tttagcgcgc aggtatccac tatcaaacag gcgctgggta aacaaggtat
     1921 cagtacgggc gcgaatgaaa agtcgctcac agtgggcacc gtgcactctc ttcagggagc
     1981 ggaaagagcg attgttatat tctcgccagt ctattcaaaa catgaagacg gcgggtttat
     2041 tgatagcgat aacagcatgc tgaatgttgc agtctccgtg cgaaggatag cttcctggtc
     2101 ttcggcgata tggatctgtt tgaggtccag ccagcctcat ctccacgggg attactggca
     2161 aaatacctct tgagtcagag aagaatgcgc tctctttgat tataaagagc gtaaggattt
     2221 aaaaaacttc cgagaccaaa atctacacac tcatggtgtg gagcagcatg ataacttcct
     2281 gaatcagacg tttcgaaaat accgaaaaca catcacgata gtttctcctg gctgacctgg
     2341 caaaagctgg agcaaaccgg ttttcttgat tctatgattg cggcgtgttc acgtggtatt
     2401 aacgtcacga tagtcactga cagaagctac aacactgaac ataaagattt tgagaagcga
     2461 aaagagaagc agcagaacct taaagcggcg ctggagaaac tgaatgcgct gggtattgct
     2521 acagctggta aaccgtttca tagcaaaatt gttattggtg atgatggttt gctatgcgtg
     2581 ggatcgttca actggtttag tgcgacacgg aagcgcgata tgaacgatac gatacctcaa
     2641 tggtttattg cggcggtgat aacctgaagg gtgagattga ggctattata atagtcttga
     2701 gaggcgtcag gtttagagca ggaatggtga gtagccatct tatcgattgt tttcgagcgt
     2761 aagatggctg aatggaatgg ctattattgc acggtcctta attataacat tctaccgaca
     2821 tgattatctt ctgtccggaa gaatcagagc tgtggtttca gactgtctgc cggtacattc
     2881 ctctccccgt taaaaaccat aacgggttca ttatcttcgt ctgtcagcgg attgaatggc
     2941 ggtatatttt cagtacgaat gccggtcagc cactgaaaaa tacctgcgaa atgacgggca
     3001 ctgatttttc tgctgacgga ctgatgagac gtgatgtcac tggcggtaat aatcagggga
     3061 acgctgtagc ctccctgcac atgaccatca tgatgaacag gattagcact gtcgctgacc
     3121 gacagaccat ggtcagaaaa gtaaagcatg gcgaaatgac gggaatgccg gcgaaggata
     3181 ccatcaagct gaccgagaaa gttatccagt ttactgatgc tggcgaggta acaggcaacc
     3241 tttcgggata ctgctccagg taatgattcg gccaggagtg aagccggtca cacgggttcg
     3301 gatgagaccc catcatgtgc aggaatcacc ttcggagagg atttatccgc cagtgcacgt
     3361 tctgtttcct gtaacaacaa catgtcatcc gttttacggg aagcaaagct gcctttcttg
     3421 aggaaaacgg tatgctccgc atcagaagca ataacagaga tgcgtgtatc atgctcccca
     3481 gttttccctg attggatatc caccatgtgc tgtatcctgc ttttgctgcc agcgccacca
     3541 cgttgttgcc ggatcagggt tctgctcata gtcataaatc agtgtccggc tcagggaagg
     3601 cacagtactg gctgctgccg atgtatagcc gtcaataaat aaaccgggag ccgtattcag
     3661 ccacggcgtg tgttggcacg ggatagccat ataccgacat ataatccctg cgcacactct
     3721 caccagtgac gataacaatc gtgtcataca acggtacacc cggcaggatt ttccagttgt
     3781 cagccccgtg ctgattcagt tgtttataac gctgcattca cgcaatgtgt cagttgtccc
     3841 cacaacagtt cctttaacca tccgcaacgg ccagctgttt actgagcata atacgaacag
     3901 cagcagtgcc agccagttac ggtgaccacg gcggtgtgtt cgccagaaaa tcaccatgaa
     3961 tacctgaatc gcgcactgac cagaaaatga taaacaggaa tcatcccggt aaactccgct
     4021 gcctcatcag ttgtggtctg caacaacgcg acaataaaac tgttgttgat tttaccgtac
     4081 gtcataccgg cagcgcatac agtgcacaac agaacagaaa taacagctgt aatggatgtg
     4141 agggtatttc tgtgtgcaag gagcagaaga aggaaacagc agcaacacat tcccgggcat
     4201 tcttctcagt gtatccgcat gcaattgtgg ttatgacaga aacaacaaaa aagaataaaa
     4261 aacaataaaa tccggggtgt attgcccgga caaaacagtt ttctgatatt catcggaata
     4321 tatcgggcaa tattattata aagggaataa gataataaaa accataaatc tcctgtgaaa
     4381 caaataaccc tgcggtatat tactgcaggg tatttctgtt agttgcagaa gatatataaa
     4441 tatcttaatt actgaatatg tgtaattatc gctcaggagc agtgtcgtca gaagttataa
     4501 gtcacaccaa acatgatgtc gtgactttta acgtcagcct ctgatttata tttatcccct
     4561 tctgcatcct tgtaagacag ggaggattta ccagcatcca gatagcgata gctgaggtca
     4621 agagcgatat ccggggttat gtcatagcga acaccggccc caatgctcca tgcgaaattg
     4681 ttatcatatc cgagtaatgc aacgcagaaa tatcgctcac ctcaccagat ttattccagg
     4741 aggtatcagt atagttgcct tatgatgtat ccgtgcataa cccagtccaa cagacaccca
     4801 tggagtgaat gcactgctgt ttctgaagtc ataatacgta ttcaccatca gggtattcac
     4861 actgagcctg ttctgtgtgt cttcccgacc accatccccc atcggagaat gccatgtatc
     4921 cagtgtataa tgggagtctg cagctccgcg gccatagaat tccacttccg tgcgcacagg
     4981 aacattgtaa tgttgataaa aatcatagcc caccgcaagg cctgcaccaa atacagtatc
     5041 gtttttatca ccgcccttat acttataaac atcatcccaa agtcctgacg gaagcgctgg
     5101 tcagtctgag tcacaaccga agcaccagct ttaccggtca cataaaagcc tgttttaccc
     5161 ctatcagcca gagcctgggc tgaaaatacg cccgccatag caagcgctga aacaacataa
     5221 cctttttcat ttcaatcatc tccgttggtt cttacccgca cgataaagaa ggttgcttat
     5281 tttaaaacca tgcaaaaaga cagggtaaaa agagaaaatt cgatcgatcg atcgacaaaa
     5341 tcgatcatgc atgatgaaga tttcttatcg aagacatgaa aatggtaagg aatggtttaa
     5401 gaggtaattg caacggcatg gaagtattga gaccagaaca gcacgagata aagcggctga
     5461 acgtcagatg agctacatgg acgtaactca gtgcaggccg cgtataatcc ataaatgaag
     5521 tggctgaaag gtgacttccc gggcatccca gatatccaca acactctatc cggctccgga
     5581 taaaccattt atggcaatat catgagcagc gttgctgaac cagatagtac tcctggttgt
     5641 atctttgaag attcaccata gaggcgactg ccgatggtca ggttttgtgt acctgctttg
     5701 taccatctca tcgttgtctc tcctgtgtcc accccgttaa ctgaaacaat ggagtcccag
     5761 ccatgcccca gaccaaccga aaatttctta ccatggctgt atgtcggagt tgtatttctt
     5821 aacagcataa aacgaatatt tgcaggcaca tcgcaagaca cagaaagagt ctgagccgca
     5881 taatgattat tagcgctatt aatagacaga tcaccatgct ttatttccag agactgtgca
     5941 gaaggacggc atccgccgat attcttaaat aagaataaca tttcattctc tcttgggagg
     6001 gttttggcca cattgtatgg gattttaaaa cgggccccca agtaactcgc gaaatgacgc
     6061 tgcatgccgg aagtgtatgg aattttaaca gaataatccc ctaaaggaag atctgcaggt
     6121 aaggcgactt taaaaataat atcgtcaaat ttctcagtca gacgagttgt ttctcctggt
     6181 ttctgacaca aattacctgc atcaaaggcc cgctcatccc acttataccc cttgagaaaa
     6241 aaatagctgt cattgtcacc agtattctcc tcactccagc tacctttatt atgaacctca
     6301 ataaataagg gatatccatt ttgggtcata acctttttgg ggaatactac ccacgcaata
     6361 tactccctgt agtaagccca ggagccatca gcgaactcag gaccattaca ctgattccag
     6421 gttaccgtag caatgcccgg gcgccacgaa gttataaatt gtggcctttg agtaatcacc
     6481 acattccctc cctgataaga gttaacgtct ccaagggagt aaaagacaat attattccat
     6541 gcagaggact caccagacac acacaaggaa aataacaaag ctgggaacca ttttttcatc
     6601 agaaatcact tccgtaatta cagttattca tttaggtatg actcagttat aaatcatgct
     6661 catactggcc gtggtccgga aatccccgcc attcagtgtc ctgctgccat tacgaaaggg
     6721 cactgaagta aaggtgaacg ttgaacgtgc tgtgtccaga cctgttgtca accggtaacc
     6781 atttcctgaa ccattaccta atgtaagagg tgttgacatt ccttttccct gatacaacgc
     6841 tataccaaaa ttggctatat ttgttgccag tacattagtc tgcccagcca ttgcactact
     6901 gtgaccttta tccagggtga gccactctta tacgtacagg atatgcttat ggtttttgtg
     6961 acttcaccac gtgagttgtc cacgtgctca ggattaatat tcccaaaatc aacaacaata
     7021 ttctgcccgt tattaatagt gcatgggggg atataaacat ttcccctgat gttaatctgc
     7081 acatcagcca gtacagcgac cgatgtcaga agcaatgata taaataatga taaacgagcc
     7141 attccccctc cggagagcgg tacagaaaat attttatttt atgagatata aaatcggcgt
     7201 attttaactg atagcattac gaatatgatg caaccagcgt tgcctgttgc agaaaatgtt
     7261 cccgcccgca aattctgtat atctccttta tatccaagtt ttgcatagag agaaatgttt
     7321 ctggccggaa cccccctgaa tatagccagg ggtaaatggc gaattaaaaa ctataggtga
     7381 accaatagaa ccatttgatt tctgttgtaa gatagataag caatccatac ctgtcgtagt
     7441 tgatgtatta ggcaccagta tagaattgta tattctgagt attcgtgtgc tgttatcgta
     7501 accttcattg ttcccagatt ataaggacac tgcatatcac agtaaactct ttttcgtgat
     7561 ttccatttga ccagggtctg aatctctaca ttcccagtaa ctctgctttg ttacagtaca
     7621 ggctggaata atcagttttc ctttaaaggt cagattatca gctgcatgtg catgctgaga
     7681 cattaacact gcccccagta ttaccggaag acacaaacct cttatctttt tcatctgaaa
     7741 tatcctgtac aaaatttttt ctaacgatat gtcaattcaa acgtggctgt tgcttcataa
     7801 tcaccgggta ccacactctt cgtccgcagg gcttccggcg ttgccacaac atacgcgccg
     7861 aaaggaagct caagactgtt tccggtaacc ttttctccct ggcctttgtt atgggaggtg
     7921 ccgggtttca gcagactgct gccatcggtg tccagcagtg caatgcctaa ccggccggca
     7981 ttcactccgg ttaccttcag atggccggga gggccgcctc ttccgttccc ttaaaggtca
     8041 gggtcacaat tttgccaact gctgttgcat ggcagttttc cagcctgatg acaaacgact
     8101 ctgtcggcga acgtccgggc ggataccaga aatccctgga cgcccgggtt ttgaagacga
     8161 catgtttatt cagactgtca ccggacacat ggcagggtct gtcaagcaga ttacccctga
     8221 atgccacatc tgaggctatt gcctgtccgg cagacagtgc ggcaaacagt aaaagagcgc
     8281 ctgtgctttt tatcatcaca ttcccttact catattttat gctcagacgc agcatggccg
     8341 gattgctcct ggcatcagaa tactcaccct cctgtgccgc ccttttcctc caggctgcca
     8401 gcatatcctc ctgccgccgg tcaggccggc acagtaaaaa ggtatcacca tcatgtataa
     8461 caagatggtc acagccggat agcttacggt caggaagtaa agcacttccg gttccgtgac
     8521 cggttaccag tgagccggag actgtcatcg caacgccccg ttttccgggc agaagtgcac
     8581 caccgtcccc acatcctgcc agcctcagca tcagaggagc tccggctgcc gcagagtgat
     8641 tctccggccg gaggcttaac ggcacctcat tactcaccag cgtgcagggt gaggacagca
     8701 gtgcaccact gactgccagg cttccggtgc gtcccccccg ttcatttatc cggtaatgat
     8761 gcaactcatc tgcagtaaag acgtcatcgt atataccccg ctcttcagcc cgcaggaaag
     8821 tatggatgaa accactcagc gacagtgcaa taagatacag tactgctgtt gttttattca
     8881 caaccataat atcccacccg catttaaccg ttattgcggt acattatttc tcttttttca
     8941 cagagcaacg gctgccatta cagataaacg acagtaccgg gcgaccacca tagtcgttaa
     9001 tataagacag ataaggggta ttataatttg ccgattttac tgtctgctct gaacggggag
     9061 acagcatcac ggtttcaaac tcaccttcct ctgcctgctt ttcacttcct cccagaccaa
     9121 taacagtgac ataatagggg gtcgggtttt caatacgata cccaccgctg actttgttca
     9181 gaattaactg gtcctgccat acttcatttg gtctggtttt aattgctgcc gggcgataaa
     9241 aaagctttat tttggtctgt aaggctatct gcagtacatt ggccttttca ctcctcggcg
     9301 gtatttcccg gagattaaaa aaaacagtga ttccctgtcc tgaggaagtt tactgatatc
     9361 cggtgtggta ctcagcctga ccatgctttt cgcacccggc tcaaggcgct gaaccggagg
     9421 ggtggcaata accggccctg taataatttt ttcctgattt tcattttcta tccatgcctg
     9481 agcaagatag ggcagttgtt tgttatcatt ggagatatca agcgtcattg acttctcact
     9541 cccgtcaaac accgcgcggg ttctgtccag cgaacagcag cgtctgcccc ggatataaca
     9601 aacaggggga tggcagccat cagaatcttt tttcgaatca tacttaattt ccacattctg
     9661 taatttcacc tggtccggaa aatggcataa ccgcattcat cagccgttaa tattccggac
     9721 tttcatttat ttctgaggcg tacagggaag caataactgc tggtcagata ttgctgtctc
     9781 cggtacattt acctgacact gtatttttcc atcccagttt accgacaggg tttcccccgg
     9841 cgtcacgcca ctcagccagg caaggccttc gtcggccacc atgcccagct cccggccttt
     9901 ttcactggtt acactggcac caaacggggg ctgagagcca tcagcaagac gcagtattgc
     9961 aaacagacgt ttccctttaa gcacgctgaa tttccggtaa ccaatggcac cttctgtcag
    10021 cgccgattcc acaacagaac gggttgcttc cacatcatcc ggtaagcgct tcaggtcaac
    10081 agaggttgta ttccggtaat aactgctgat gtcagtcacc acgcccgttc cccagcgatt
    10141 tgtcaccacc tgcccgccat caaccggtac acctcccaca ccatccgtgt caacaagaag
    10201 acgtgttcca ccggacattc cccctgcatg taacgccgca ccttttccgg taattgttgc
    10261 cccaccggaa gcactgacgc cgaaagacgt atatcctttc tgcagggatg caatattcgc
    10321 ggacaaattt gccagcggac tacgatgact gtaataggca ttaatctgac gttgcgatgt
    10381 cagtccaccg ccactgttaa ggccggcgtt caggctgtag ctgtccagac cgtcattgaa
    10441 catgtcagtg tagccggcca tattcacata acggtcatta ctcatactgc cactgtagct
    10501 cgctgtcccc gtccccagcg gcacggatat acgcaggtaa gcagaatcgt tatcacgccc
    10561 cagatattta gaccttgagg ctgacaatcc aaccgccaca ccctgcagtc cgaaaacatt
    10621 aaagtagcgg ttgacgctca ccgtataata gtccgttttc cgtatgtccc agtatgtctg
    10681 acggctgtac tgcaggttaa aagaggtgtt ccagtccgcc acgtttttat tcagcgtaac
    10741 ggtatacatc tctttttccc gactgctgta atcattacgg tagcgggcgt tcaggtactg
    10801 ctccatggtc atatagtttc gctctgagaa acgatacccg gcgaacgtaa tgtcggcatc
    10861 cgcattatca aaccgtttgg agtagctaag acgccaggat tttccctgaa acgttctctc
    10921 tccctcaata cgggctactg actgcgtgat atcagcggaa agggtccccg gcacacccag
    10981 gtcccagccg gcaccggctg ccagtgcatt ataatcaccg gcaagcacag ccccgccata
    11041 cagcgaccac tggttactga gcccccagga cgcctctccg gtcgcaaata caggcccttc
    11101 ggtctcatgc ccgtatccgc gggagcgacc ggagacaagt ttataccgga cctgtcccgg
    11161 acgcgtcaga taaggaaccg aggccgtatc gacctgaaag gttttcttcc gtccgttctg
    11221 ttcaataacc tcaacatcaa gacgtccgcg aactgaactg tccaggtcct gaatactgaa
    11281 tggcctgcgg ggaccatcga gtcgtacagc acccgtccct gctgcgacac cacaacacgg
    11341 gcattagtct ccgcaatccc ggtaatctgc ggtgcatagc ctcgcagtct gggtggcagc
    11401 atccggtcat cgctttccag gctggctccc gtataactcc atgaccggaa tatatctgag
    11461 ttgatattat tctcgcccag cgtcaggttt gcccgccatc gtggaatggc acggaacaga
    11521 taaaagcgac tccatgtgaa atttctgttt gtcgtttttt ccccgttgta gcggctctgc
    11581 tcctggcttc ctgatagtca gcccgcaggc gccagggccc cagattcccc ccacagtccc
    11641 gttataactg aactgatgag agtctcctcc ctgataatta cgggaaaccg tcccgttgag
    11701 gttataatcc agcatcagtc cgggaatgcc gtcgtcccag cgtgagggag gcagccaggt
    11761 ggcatcagaa tactcaagcc aggctgcggc atattgatgc gtaatacgcc cgccccggta
    11821 tcaggacgaa tatccactcc cggcaaccca tgaaaatccg cacactgacc atcatgccag
    11881 taaacaactt tatccagaga ttctgctgtt aaccccatca gtctgaccat atctgatgtc
    11941 aggcaggcct gtggcaattt tttttctgcc ttatctcctg acgactgagg ttcaacaaat
    12001 gaaatctgta acgatgcggg agaaatactt tgcccgttaa caatcacatc cagaagatat
    12061 tgccccggca gaacataacc ggcttctgaa aaacgggtga agtcaatatt tttcttgtcc
    12121 gccgcgtcaa gtacatctgt attaaactca acggcactgg ctgcgttaca aaacagagac
    12181 aacaatatca cacaggtaat attgttgact gcaaaaggta ttctgtcttt cattccacgc
    12241 atcaccagat tcacaaaaaa gataaataac ccggacatct caccggagtg actcactcat
    12301 aatcgacccg gaatcccagc acggcaaaat aattttccgg cttcaagttt tttttccgtt
    12361 tcgcacaatt ctgagggtgt aacccagcgc ttcttcatta cccgtcaatg gtattgcagg
    12421 cattactttc cctgcccggg caatatttcc cctggcatca gctatctgca gattaattcc
    12481 tttgcctgac cggataaatt aaacttatcc ggcgtttcac cccggacgcc atcgaaagtc
    12541 acccttatcc gggaatcaga gaaaaggttc ccaccctgac tgttaaattc acagttcctg
    12601 agccggaggc tgaattttct ttcaggtccg gagaaaccat tctgtaaatc ccgaaccggg
    12661 ctttccccca tatcaataat ctgccaggcg tcttccatcg ccagagtaca ggcaggtctg
    12721 acaacctcac catgaaaagc agccctgccg tcccaccata cgtgttcttc tccccagtat
    12781 tcaggaaggg acatgccggg cggaggaaat ggctctgaag atacagcatg aagtaatgta
    12841 ctccagaaat acatcacaaa cagaacacaa aaattcttca taccttaaat gtcgttgtca
    12901 gacacagggt gggtcttgcc tcaccctgta aaaatcagat gaattactgg taacttaaat
    12961 taaaggttgc gactgcagaa aaagcaccct cagtaacttg ggcattattg gctgacgact
    13021 tcttacatcg cagtataatg cagcacatta tcaccatctt tcagggtatt agcatcacct
    13081 tctgtgccat cgaaagaaac gtttttacct gcagctgaac tacaatcgca gtacctgtcc
    13141 ccccgctggt actaactcct cattagacca gaaacccttg gaccactaaa agccagcttc
    13201 acagtccctt ttttttgctg ccggggctcc tcccaccctt aaatgaagtg atatcacaat
    13261 taccaattca atatctaaat tcattggttg gatacgcctc ctgcttgaag gaagcttttt
    13321 gaaagctgtc caaaatcata gactgatcag ctgatttctg agaaatgctg catggagcat
    13381 caacaacagt tccgttaaag gttactctac cctgcccctg tggagtagtt ggagcagcat
    13441 ttacaccaaa agacaccact gccatagcta ccgcaccggc aataaccgac ttaatcataa
    13501 ataacaacct ctttttcatt actgaacaca ccagagatat ctggcaattt aaatgacccg
    13561 attaccttta ataaccagat caatacaaat atattctcgt attttcaggg caggttattt
    13621 atcgacagaa tactgatata acctgtctgc tttctgataa gggcttcttt tttatcttcc
    13681 agaaactccg gaatgccata attagtcaaa tgccgacgac tcatctgtat aataaggtgc
    13741 aagccttgct gcaagagcat tcagccgtat aagtctccca gtgttgtact gaaataccca
    13801 ttattcatct ggtatttctc gcagacctcc ttacggagtg cccacctacc agatagtcct
    13861 tcatagccag aatgaccctg tcactgtgaa tagaagaaat acctatcagt aaaaaaaatg
    13921 catttcagac atagagccgg gcaacagtac gctctcgcgt atattcagca aaaacgcatt
    13981 tcctgaccga ctgatgactt catgatgcgc catgtttccc ccttctgtcg ggcccctgga
    14041 tatatgcttc cggcccggaa atactcttca cgcaataagt taaatttagt ttttatgttg
    14101 taaatattga tttaaacgat cttttaaccc gcaaaacaaa accaaaccag aataaaacac
    14161 caacaacaag aatacactca gataaaaaca tcatgtgaat tgaatttaca gcataaaaga
    14221 tcgtctaaat gcatttttac ggactttctt tcacagaaaa taacacacaa aatgtgatat
    14281 atcatcaata aagccaacaa aaaagcaaat tcactaaatt tcacaaataa agcaaaccca
    14341 gagaataaaa ataccttccg ttgataacag agagcatgat ttttttattc gaagaaactt
    14401 tgttacagtg agatgagtga atatatgaga atgaaatact ggaatttctg aacaggcata
    14461 atggagggaa aaccgcagaa attgcggagg cgctggcggt aacggattac caggcacgct
    14521 attacctgtt attactggaa aaagaaggta tggttcagcg ctctccatta aggcggcatg
    14581 gcacatactg gtttttgaaa ggagagatgc aggcgggaca aaactgttct tccacaactt
    14641 aataactcag tttgtgggca tgcttcactg aaacagataa aagctacatc agagtgcaga
    14701 ttcaattttg cagcattcgt cacaaaataa aatccagcat tttatgtcag aatacctcat
    14761 gataataaaa cctgtccggt tttactgcat ggccagacat tataaaaaag caaatccgta
    14821 actccattca gcctccttgc tgtccggtaa cggaggctgt ttttttcgtt acgcctttcc
    14881 ccggcagcat cagtagcagt aatttataga ttctacgaac tgagactgtc tgtccaggga
    14941 gacatacgca tcccggcgca atgctccggt catttacaac ctgcttctga tcataaacgc
    15001 cacgatcctt ggctctcaca gatttcggga gaagaaagtt agtttacagg cagaaaccac
    15061 tcataatatt gacatgagaa agattttctg ttatacaaaa aacatatgaa atgatataac
    15121 atcacaaact ggagttttta tggcagaatc cgggtttatt tctctcgaat atcccgctca
    15181 caggcgcctg ttacaattag caagacagct atactctttg cagaagcaga caatgatacc
    15241 ccggagtcaa ctcatctaca ttgcgttggt ggtatcactc ataccgtaaa tggtgttttt
    15301 aacgatattg cgaattactt ccgcgattca ttaagacgca ccgtcgggat aatggtcagc
    15361 ccgcagtggc tatccatgac tggaatgttt catacattga aaaaggcaac gatgcttctg
    15421 ctgttgttta cttcacagaa tcaggctcaa gtataaccgt ccgtgaatct tacgaagaca
    15481 taatagcaaa gtttcacgct ctttagtcaa cacctgtgct gagaattaaa gcaggggcag
    15541 gatacaatta tcctgcccct gccgttaaag attatacaat gagtattact ttgtaatatt
    15601 tcggtggtgg gttataaaga tgtggtctcc tcagaggatc gtttatggca gatagtgcag
    15661 tattgcttat gaaaatgaac aattcagcat gagcatcaga ctaaaaaatc gtatccggaa
    15721 tagactccct gaaacaggat tctgctataa ctgcggagaa cctgttaaga ctggattgtt
    15781 ttgtgatggt gactgtcggg aagattatga gaaacgtgaa cgatttggac aaataaatac
    15841 tgacaatgtt taaaaagcaa tacaattact gatatctctt tcagacttca gaattaaaga
    15901 aactgcccga atgttatccg aaaagcagct gtaattagta atgctcttat cagagtgaaa
    15961 catcacctcc cagaatacat cagaacagaa ggataacagt tatccgatgt gacggctata
    16021 atgggccgac acagagggtt actcaccgat ctccttcacg gtaatacgca gctctgagca
    16081 tggtggtgag cggggagtgc tactgtacag cctgatcggg acgtgcaaac tgaacgacga
    16141 ggatccagaa aactaccttc gccatgtgtt tggcgtcata gctgactgcc ggtcaaccat
    16201 gtcagcgaac tgcttccgtg gcgctagcac tgcccgctga ataacacatc cccgtcaata
    16261 cggccctcgc tgtactctta ccgtggtctc agcagtccac ttatttgcat gaataaacga
    16321 gtggtcgcag acacgcaaca tcattcaaat ccccccgcta atattacagg taacttcagt
    16381 gtgtatggaa acacctcatt aactgttatc acaaataaaa cgcatacctg ttccatatga
    16441 aatttatcac gcctgactac ataacattaa ttatccggag aactgggatg ccgtgacaca
    16501 gcatgcacag aaatccccgt cgcaacagcc tttatcagaa aaccatcttt ttaccggtct
    16561 atggtgttgc agagatattt aatactgacg ccgcgcatac catttttctc catcgcattg
    16621 taaagatgcg cacataccgt cttcatgtta tcccagccgg tctgccagaa aggtaaaccc
    16681 gtagtcatgg aaaacaaccc cgaagaccac caccatctct gtccgggtca gtggccttgg
    16741 ccgtatgtcc gaacagccac gctccacggc ggatggtttg cctgatatct gtcaggcatc
    16801 tcacagagga aacaggaatg acaagaaacc gacgcccaca gaacatagag tatgtaagcc
    16861 tttccagatt acggtcacag agaatcacat aactgtcttc gggaagtgac tgacgttgct
    16921 ttccggcatt aaacagctct ctgcctgtcg tgacctgata tccgctcagt atacgcatca
    16981 ggccatattc cagatatctg tcccgggtca taataataat ccattactct ctgccccttg
    17041 tatcccgata ccggctccgt ttaccattac cggtataact gacttatcat tcataacgga
    17101 aactgtctgc cgcatacatc agcgcccgct cttccagctc tgaggctcag ttctttcagt
    17161 tccccttccc cttcagcaag aagataacat tcaaaacggc ttcatcgtcg cattccagac
    17221 gcacctgcgc cctgtcagcc cgaccgttta caataatagc tgcactgacc ttatgcgata
    17281 gttcagcata tacactcttc ctcagatctt cagaataccc gggaacagtc agttatgcca
    17341 tcggggagaa aaacaggcag cgaaatctta cttcataccg aaaactgcct cccggtgact
    17401 caggcccggg ttcagaacta ccagttgaca cccacattcc aggaaacata gccgccactg
    17461 aaagtagtac ctgcctttaa gcagtctgct tggcactata cccgccacag ccgctatg
//
