LOCUS       CP088455                5166 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0052 plasmid pF16EC0052-3, complete
            sequence.
ACCESSION   CP088455
VERSION     CP088455.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391677
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 318x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:34:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,232
            CDSs (total)                      :: 5,119
            Genes (coding)                    :: 4,888
            CDSs (with protein)               :: 4,888
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 73 of 231
            Pseudo Genes (incomplete)         :: 176 of 231
            Pseudo Genes (internal stop)      :: 42 of 231
            Pseudo Genes (multiple problems)  :: 50 of 231
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0052"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0052-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(4532..5166,1..280)
                     /locus_tag="LQR01_26140"
     CDS             join(4532..5166,1..280)
                     /locus_tag="LQR01_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGE59710.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(484..882)
                     /locus_tag="LQR01_26145"
     CDS             complement(484..882)
                     /locus_tag="LQR01_26145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE59711.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1059..1985
                     /locus_tag="LQR01_26150"
     CDS             1059..1985
                     /locus_tag="LQR01_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGE59712.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1988..2521
                     /locus_tag="LQR01_26155"
     CDS             1988..2521
                     /locus_tag="LQR01_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE59713.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2587..2796)
                     /locus_tag="LQR01_26160"
     CDS             complement(2587..2796)
                     /locus_tag="LQR01_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE59709.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          2..280
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.56e-144"
     Typing          4533..5166
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.008"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1060..1982
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caatgcatcg cttcccgttc cgctttcact ggctgaatgt cgggctattg gcaagagcat
       61 tgcgaaatac acgcacagga acttcacgcc ggaaactttc gcacagtatg tggctgatac
      121 gcacacgcca gaaattcagg ctgcacgcgg tcgcaagggc ggttctaagt ctaagcgcag
      181 cacagtagct acatctgcac gcacgctgaa accgtgggag gcattaggga tcagtcgcgc
      241 ctggtattac caactgaaaa aacgaggtct cgtagagtag accaaataag cctatatcag
      301 ataacagcgc ctttctggcg tctttttgag cagcaggttt tttgccgttt ttccccgttc
      361 agccctgcaa tggcgcggct ttccgcgtga ttgtggtggt agtgctcgcc gcagtctcat
      421 gaccgagcgt agcgagcgaa tgagcgagga agcaccaggg aatcggaaca cggatggagg
      481 cacttacgcg tctgggtttg gggatgtctc cagatcgtac ttaagttcct taagtgcttg
      541 taatatcctg tcttttttat agcatagctc gcctgttttt gtgtctacca gtcctgcttc
      601 gatcagtagc ccagccgcga gaaagagccg gtgattgcgt tcccgtcgtt gtttttttcc
      661 ctcgattcgt ttttggaccg cctgttttcg ttcgctctcc tgtgcctgta agtcgttttt
      721 ttccgctgtt gccagtacgt ggagaagttt gcgttcctgt tctgtgagtc cggcttcgtt
      781 gtcgatcagg tcaataatct gtctctgtcg tgcggtctgt tctccgagct tcctgatgaa
      841 tccgatccgt ttcttcagcc attcgatgtt gctgatgcgc atgttcgtga tgctccctgg
      901 tgtttttttg cggtgtgttg tggtcgtgca gtatatcgct tgcggtcggc gggtgcaggg
      961 ggtatcttcc gtgctaattc gtaaaattag aaggaagcgc acttatatgt ttctctcctt
     1021 cgtcgagaaa cagtgcgctc ctgcagaggg ctgaaaccgt ggcaacctat cacctcagcg
     1081 ttaaatttgg tggcaagggg caggctgcaa accacgccga ttacatcgag cgcaaagaga
     1141 aataccgcga ccgtcaggat ttggaatatt ccgcacacgg aaacatgccg gaatgggcca
     1201 gggataatcc ttcccatttc tggcaggcgg ctgaccagtt tgaacgtgcg aacggttcga
     1261 cctataggga acttgaaatt gcattacccc gtgaactgac accggaacag cgccttgaac
     1321 tggtgcagga ttttgtacgt caggaagctg gcgaacggca cgcctggagt tttgccattc
     1381 acaatccgaa agcgagcatt gacggcggcg aacagcctca tgcgcatatc atgatgagtc
     1441 agcgtgtgaa tgatggcatt gaacgtacac cggaacagta tttccggcgc tataacgcca
     1501 ggtatcccga gcggggaggt gcgaaaaagg attccggaag cctgactctc actcagcaaa
     1561 aagagcagtt gcgggaattg cgaaaacgct gggaggtgaa gcacaacgaa catatgagaa
     1621 agcatggctt tgaaaggggc ttcattgact gccggacgct gaaagaacag ggcattgaac
     1681 gacgtcccga agtgcatctg ggttttgaag ctgccggacg gcttgatgat gcccagcgtc
     1741 atgacatcat gcaaaaacgg tctgaaccgg actattcccg ccatcttgaa caggcgcgaa
     1801 cggtcgggca tgacatcagg gaatggctgg acactccggt taagcaacat gaactggtca
     1861 gaaggctgca acatgatgtt gcgcgggagc atggctttga catgagagac agagtgagca
     1921 gacagaaccg aactgcacct gaacgggtta aatcaccgtc acgtggattt tctatgggat
     1981 tctgaacatg gccagcacag aagaaaaatt actcacgctc ctgttatcgg tggagcatct
     2041 gcaacatacc gcaatggagc aacagaaagc tgttgctgat tctctggctg tggcaaggga
     2101 aactcttcgc ggatatcggg atatttctga cggtgtggca aaacaggtca gggatgaagt
     2161 cagaaatgag gtgcagaagc tggacgtggc aggactgata ggtgagcgca tcagccagga
     2221 ttttcagcgg ctggaagagt cagtcgcaaa catgaaggca aacgcggatg ccctgaacag
     2281 ggatattgcg acaattcaca gcaatatcgt gcaggaatac aacaccctta aagggtatag
     2341 ctggaaatgg ctgggtgggg tgtcagtggc ttttctggtt atgttctgtg tgctcggatg
     2401 gggaatgaaa acaatactcg acaggaacta caacgccaca gcggcggtgt atcagaaagt
     2461 tgtgacactg gaagataaac tggtggagaa acctgtcacg gggaagagcc ggaaaaactg
     2521 acgcgcttcg cttgtccaac gcctcacgac ccgcgaaagg atttcgcgaa ccgttcaccg
     2581 tttttttcaa tatgattctg tctgctttgg tcgataaagc agacgatagt attccagcct
     2641 tcgtatatat aacggtacgt gctcatcagt aagagctggc ggtattgtgt tatcaggggg
     2701 aagttttttc agatactcac gaaatgcctg gcttgatgcc ataaaatcta cagtagcatc
     2761 gataatatgt ggggtgtttt cacctaattt tgccatgatg ataatcctta tattatcacg
     2821 gtttgtgaat gatagggtgt cactattatt tatgcgttag tgaaccagaa aaaatccccc
     2881 gttggcatag ggggatatgt aaggagaaat aacttcgcag gtgttcttgt ttgtataacg
     2941 tgaaaagact gcacgcaact taataagtct gccgtactta ctttcagttt tcaacataag
     3001 gtttataaca atgtatggaa tggaagtgag ctttaaggtt tcagctctaa aaaataaaat
     3061 atttattagt tggctggttg ttgttcttgt tctgtatgta tataaacact atcgacatat
     3121 tatgaccggg aacattcctt accccctctg cgctcgcaga ggtttttttt gtcattaatt
     3181 aataatgaac tgaaatgcag ttccgatacc tccatttgca cattatccct gacatatggt
     3241 accggaaccg cattaatttt atgttataag agtttatttt taatgtccag atggtttaat
     3301 agtatttata tgtaattagc tgtatggata gtactggttt tattgtataa cttgtcgttt
     3361 tttttattgt ttttgtgtgg tgtgctaatc tttaagttat aaaaaaaccc cttggaatac
     3421 aggggcaaaa gtaaggaaaa caaatggatg ctcagtttgt gtgacacttt aaactaatgg
     3481 aactatagag tggaggttgt tacagtcgct tttataaaac agcaaccagt ttttttcaat
     3541 agtctgatga tggttgtgac agttttgtga tattgttcaa aatgtttctt gttgaatgtt
     3601 tgttttttgg tctattggtg tctggtttta ttttttctgc tattacatat caagaaatgt
     3661 gattggtgta gcaagagtgg aatgcgatgt ggataaacta attggttgtc gaatctaatt
     3721 tacttagaaa actacacttg tttttgtgta tgaatttagt gaatttgtct gtctatgtgt
     3781 tttcgtgcat ttataaatcc ttcgtcagga tcttaaattt agtgttgaat atgtagggcc
     3841 tgtacggtaa ttattattta gcgtagctac ttcatcgttg ttcgaacaca accaacaacc
     3901 tgtaattgtt gtttctcttg gttccccact tttgtggggt tttttatctc ttccgtgcat
     3961 cgtaaagcgc ggactccttc tggaaaggtt gttttagggc atcactgagg cattttttgc
     4021 gtttgtgaat attgagcatc cgataactaa aatatttccc atagactctg attcttgcca
     4081 ccgaccccag gtcggttttt ttttgtcctg gcgagatgtg aagtttggaa acaggaaacc
     4141 ccgtcagagc gccacgcacc aggggagaaa acttgtcgtt ttgagccgtt cggtgttcag
     4201 aacgcacgaa accgatcgca catcgctttc gtgcatagtt atgcaacgcc ctgaaaacga
     4261 ttctgacgcg tttttctggt ttggcctggt gttttcttgt ctttttgcgt tttttgcgcc
     4321 agaaagcgac tgagggcgtt ttaagggggg cgtacaacgg gagttatggt aaatggatcg
     4381 ggttttcggg aaggttcgac aggatttgcc gttgggtgta gtgtaagcga ctgaaaaaca
     4441 aacgcccctg aaatcatgtc cagatccggc aagattcaac atgaaataca gagggcgtgt
     4501 aggtcgcata acccggagag aatcgtaaca catgagcgcc gcgcttcaat acttcgacga
     4561 aaatttaccc catcgtccct atcacacgga tgatctcgct tttggtcttc gcatctccgg
     4621 caaagggcgt gcgcttcttg cgcggtacat acagcagaac cagcctcatg cgcagttctg
     4681 gctggttttt gacgttgacc gcgagggggc tgcgattgac tggagcgacc ggaacgctcc
     4741 cgcgccgaac atcaccgtta aaaatcctgt gaacggacac gctcacctgc tctacgcact
     4801 caacatcgcc gtgagaaccg cgcctgatgc ctcggttaag gcgctgaaat atgccgcagc
     4861 ggttgaacgt tcgctgtgtg agaaactttg tgcggatgtg aactacagcg gcctgatttg
     4921 caaaaatccg ttccacctgg aatggctggt gatggagtgg cgcgaggaag cctataccct
     4981 cgatgaactg gctgattatc tcgatttgag cgcctcagag cgtcgtagca tcgataaaca
     5041 ttacgggatg gggcgaaact gccacctgtt cgaaatgacg cgcaaatggg cttacagggc
     5101 gattcgtcag ggctggccag cgttctcaca gtggcttgaa gcggtgatcc agcgtgtcga
     5161 aatgta
//
