LOCUS       CP088448                5171 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0184 plasmid pF16EC0184-5, complete
            sequence.
ACCESSION   CP088448
VERSION     CP088448.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391679
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:34:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,086
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 78 of 207
            Pseudo Genes (incomplete)         :: 141 of 207
            Pseudo Genes (internal stop)      :: 36 of 207
            Pseudo Genes (multiple problems)  :: 41 of 207
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0184"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0184-5"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4403..5171,1..146))
                     /locus_tag="LQT15_25410"
     CDS             complement(join(4403..5171,1..146))
                     /locus_tag="LQT15_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGE49761.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            1887..2096
                     /locus_tag="LQT15_25415"
     CDS             1887..2096
                     /locus_tag="LQT15_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49762.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2162..2695)
                     /locus_tag="LQT15_25420"
     CDS             complement(2162..2695)
                     /locus_tag="LQT15_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49763.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2698..3624)
                     /locus_tag="LQT15_25425"
     CDS             complement(2698..3624)
                     /locus_tag="LQT15_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGE49759.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3801..4199
                     /locus_tag="LQT15_25430"
     CDS             3801..4199
                     /locus_tag="LQT15_25430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49760.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(4404..5171)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.549"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2702..3624)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactgcgcat gaggctggtt ctgctgtatg taccgcgcaa gaagcgcacg ccctttgccg
       61 gagatgcgaa gaccaaaagc gagatcatcc gtgtgatagg gacgatgggg taaattttcg
      121 tcgaagtatt gaagcgcggc gctcatgtgt tacgattctc tccgggttat gcgacctaca
      181 cgccctctgt atttcatgtt gaatcttgcc ggatctggac atgatttcag gggcgtttgt
      241 ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga
      301 tccatttacc ataacgtcgc attgtacgta cccattaaaa cgccctcagt cgctttctga
      361 cgcaaaaaaa cacaaaaaga caaggaaaca ccagggtaaa cataaaaaat gcgtcagaat
      421 cgtttttagc gcgttgcata actatgcacg aaagcgatgc gcgatcggtt tcgtgcgttc
      481 tgaacaccga acggctcaaa acgacaagtt ttctcccctg gtgcgtggcg ctctgacggg
      541 gtttcctgtt tccaaacttc acatctcgcc aggacaaaaa aaaccgacct ggggtcggtg
      601 gcaagaatca gagtctatgg gaaatatttt agttatcgga tgctcaatat tcacaaacgc
      661 aaaaaatgcc tcagtgatgc cctaaaacaa cctttccaga aggagtccgc gctttacgat
      721 gcacggaaga gataaaaaac cccacaaaag tggggaacca agagaaacaa caattacagg
      781 ttgttggttg tgttcgaaca acgatgaagt agctacgcta aataataatt accgtacagg
      841 ccctacatat tcaacactaa atttaagatc ctgacgaagg atttataaat gcacgaaaac
      901 acatagacag acaaattcac taaattcata cacaaaaaca agtgtagttt tctaagtaaa
      961 ttagattcga caaccaatta gtttatccac atcgcattcc actcttgcta caccaatcac
     1021 atttcttgat atgtaatagc agaaaaaata aaaccagaca ccaatagacc aaaaaaacaa
     1081 acattcaaca agaaacattt tgaacaatat cacaaaactg tcacaaccat catcagacta
     1141 ttgaaaaaaa actggttgct gttttataaa agcgactgta acaacctcca ctctatagtt
     1201 ccattagttt aaagtgtcac acaaactgag catccatttg ttttccttac ttttgcccct
     1261 gtattccaag gggttttttt ataacttaaa gattagcgca ccacacaaaa acaataaaaa
     1321 aaaacgacaa gttatacaat aaaaccagta ctatccatac agctaattac atataaatac
     1381 tattaaacca tctggacatt aaaaataaac tcttataaca taaaattaat gcggttccgg
     1441 taccatatgt cagggataat gtgcaaatgg aggtatcgga actgcatttc agttcattat
     1501 taattaatga caaaaaaaaa cctctgcgag cgcagagggg gtaaggaatg ttcccggtca
     1561 taatatgtcg atagtgttta tatacataca gaacaagaac aacaaccagc caactaataa
     1621 atattttatt ttttagagct gaaaccttaa agctcacttc cattccatac attgttataa
     1681 accttatgtt gaaaactgaa agtaagtacg gcagacttat taagttgcgt gcagtctttt
     1741 cacgttatac aaacaagaac acctgcgaag ttatttctcc ttacatatcc ccctatgcca
     1801 acgggggatt ttttctggtt cactaacgca taaataatag tgacacccta tcattcacaa
     1861 accgtgataa tataaggatt atcatcatgg caaaattagg tgaaaacacc ccacatatta
     1921 tcgatgctac tgtagatttt atggcatcaa gccaggcatt tcgtgagtat ctgaaaaaac
     1981 ttccccctga taacacaata ccgccagctc ttactgatga gcacgtaccg ttatatatac
     2041 gaaggctgga atactatcgt ctgctttatc gaccaaagca gacagaatca tattgaaaaa
     2101 aacggtgaac ggttcgcgaa atcctttcgc gggtcgtgag gcgttggaca agcgaagcgc
     2161 gtcagttttt ccggctcttc cccgtgacag gtttctccac cagtttatct tccagtgtca
     2221 caactttctg atacaccgcc gctgtggcgt tgtagttcct gtcgagtatt gttttcattc
     2281 cccatccgag cacacagaac ataaccagaa aagccactga caccccaccc agccatttcc
     2341 agctataccc tttaagggtg ttgtattcct gtacgatatt gctgtgaatt gtcgcaatat
     2401 ccctgttcag ggcatccgcg tttgccttca tgtttgcgac tgactcttcc agccgctgaa
     2461 aatcctggct gatgcgctca cctatcagcc ctgccacatc cagcttctgc acctcatttc
     2521 ggacttcatc cctgacctgt tttgccacgc cgtcagaaat atcccgatat ccgcgaagtg
     2581 tttcccttgc cacagccaga gaatcagcaa cagctttctg ttgctctatt gcggtatgtt
     2641 gcagatgctc caccgataac aggagcgtga gtaatttttc ttctgtgctg gccatgttca
     2701 gaatcccata gaaaatccac gtgacggtga tttaacccgt tcaggttcag ttcggttctc
     2761 tctgctcact ccgtctctca tgtcaaagcc atgctcccgc gcaacatcat gttgcagcct
     2821 tctgaccagt tcatgttgct taaccggtgt atccagccat tccctgatgt catgcccgac
     2881 cattcgcgcc tgttcaagat ggcgggaata gtccggttca gaccgttttt gcatgatgtc
     2941 atgacgctgg gcatcatcaa gtcgtccggc agcttcaaaa cccagatgca cttcgggacg
     3001 tcgttcaatg ccctgttctt tcagtgtccg gcagtcaatg aagccccttt caaagccatg
     3061 ctttctcata tgttcgttgt gcttcacctc ccagcgtttt cgcaattccc gcaactgctc
     3121 tttttgctga gtgggagtca ggcttccgga atcctttttc gcacctcccc gctcgggata
     3181 cctggcgtta tagcgccgga aatactgttc cggtgtacgg tcaatgccat cattcacacg
     3241 ctgactcatc atgatatgcg catgaggctg ttcgccgccg tcaatgctcg ctttcggatt
     3301 gtgaatggca aaactccagg cgtgccgttc gccggcttcc tgacgtacaa aatcctgcac
     3361 cagttcaagg cgctgttccg gtgtcagttc acggggtaat gcaatttcaa gttccctata
     3421 ggtcgaaccg ttcgcacgtt caaactggtc agccgcctgc cagaaatggg aaggattatc
     3481 cctggcccat tccggcatgt ttccgtgtgc ggaatattcc aaatcctgac ggtcgcggta
     3541 tttctctttg cgctcgatgt aatcggcgtg gtttgcagcc tgccccttgc caccaaattt
     3601 aacgctgagg tgataggttg ccacggtttc agccctccgc aggagcgcac tgtttctcga
     3661 cgaaggagag aaacatataa gtgcgcttcc ttctaatttt tagaattagc acggaagata
     3721 cccccctgca cccaccgacc gcaagcgata tactgcacga ccacaacaca ccgcaaaaaa
     3781 caccagggag catcacgaac atgcgcatca gcaacatcga atggctgaag aaacggatcg
     3841 gattcatcag gaagctcgga gaacagaccg cacgacagag acagattatt gacctgatcg
     3901 acaacgaagc cggactcaca gaacaggaac gcaaacttct ccacgtactg gcaacagcgg
     3961 aaaaaaacga cttacaggca caggagagcg aacgaaaaca ggcggtccag aaacgaatcg
     4021 agggaaaaaa acaacgacgg gaacgcaatc accggctctt tctcgcggct gggctactga
     4081 tcgaagcagg actggtagac acaaaaacag gcgagctatg ctataaaaaa gacaggatat
     4141 tacaggcact taaggaactt aagtacgatc tggagacatc cccaaaccca gacgcgtaag
     4201 tgcctccatc cgtgttccga ttccctggtg cttcctcgct cattcgctcg ctacgctcgg
     4261 tcatgagact gcggcgagca ctaccaccac aatcacgcgg aaagccgcgc cattgcaggg
     4321 ctgaacgggg aaaaacggca aaaaacctgc tgctcaaaaa gacgccagaa aggcgctgtt
     4381 atctgatata ggcttatttg gtctactcta cgagacctcg ttttttcagt tggtaatacc
     4441 aggcgcgact gatccctaat gcctcccacg gtttcagcgt gcgtgcagat gtagctactg
     4501 tgctgcgctt agacttagaa ccgcccttgc gaccgcgtgc agcctgaatt tctggcgtgt
     4561 gcgtatcagc cacatactgt gcgaaagttt ccggcgtgaa gttcctgtgc gtgtatttcg
     4621 caatgctctt gccaatagcc cgacattcag ccagtgaaag cggaacggga agcgatgcat
     4681 tgtacatttc gacacgctgg atcaccgctt caagccactg tgagaacgct ggccagccct
     4741 gacgaatcgc cctgtaagcc catttgcgcg tcatttcgaa caggtggcag tttcgcccca
     4801 tcccgtaatg tttatcgatg ctacgacgct ctgaggcgct caaatcgaga taatcagcca
     4861 gttcatcgag ggtataggct tcctcgcgcc actccatcac cagccattcc aggtggaacg
     4921 gatttttgca aatcaggccg ctgtagttca catccgcaca aagtttctca cacagcgaac
     4981 gttcaaccgc tgcggcatat ttcagcgcct taaccgaggc atcaggcgcg gttctcacgg
     5041 cgatgttgag tgcgtagagc aggtgagcgt gtccgttcac aggattttta acggtgatgt
     5101 tcggcgcggg agcgttccgg tcgctccagt caatcgcagc cccctcgcgg tcaacgtcaa
     5161 aaaccagcca g
//
