LOCUS       CP088447                8200 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0184 plasmid pF16EC0184-4, complete
            sequence.
ACCESSION   CP088447
VERSION     CP088447.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391679
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:34:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,086
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 78 of 207
            Pseudo Genes (incomplete)         :: 141 of 207
            Pseudo Genes (internal stop)      :: 36 of 207
            Pseudo Genes (multiple problems)  :: 41 of 207
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0184"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0184-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(7573..8200,1..38)
                     /locus_tag="LQT15_25365"
     CDS             join(7573..8200,1..38)
                     /locus_tag="LQT15_25365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49754.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTSTL
                     EREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLNDV
                     KKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLFLLL
                     MIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDCVSFGNGFSVCV"
                     /name="hypothetical protein"
     gene            complement(131..580)
                     /locus_tag="LQT15_25370"
                     /pseudo=""
     CDS             complement(131..580)
                     /locus_tag="LQT15_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /name="conjugal transfer protein TraD"
     gene            752..1837
                     /locus_tag="LQT15_25375"
     CDS             752..1837
                     /locus_tag="LQT15_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGE49755.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKARF
                     GETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGPVRVA
                     VMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALVERDNIPPSLDELGAELK
                     AFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDLERERER
                     VRQRAYSSPAPSRSRDYGMSR"
                     /name="MobA/MobL family protein"
     gene            1858..2574
                     /locus_tag="LQT15_25380"
     CDS             1858..2574
                     /locus_tag="LQT15_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49756.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTSTL
                     EREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLNDV
                     KKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLFLLL
                     MIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVDDESG
                     YYFNEQSPNKKYAILK"
                     /name="hypothetical protein"
     gene            complement(2632..3204)
                     /locus_tag="LQT15_25385"
     CDS             complement(2632..3204)
                     /locus_tag="LQT15_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="UGE49757.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFLHGLKSSIKTSIY
                     IRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLINKKNKQRKEQKPF
                     LIPLLNPKAYLFFAALIPTFIDNNTNITLNFFILGVLFIFISFLTDLIYIAISLTIRDK
                     LTPSFSRYISICSSIFILGTGIYFIFT"
                     /name="LysE family translocator"
     gene            complement(3211..4161)
                     /locus_tag="LQT15_25390"
     CDS             complement(3211..4161)
                     /locus_tag="LQT15_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49758.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFHS
                     YDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTIVIKYAITLNKKLNTWCLGMAE
                     GAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNSNLK
                     QFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKREIQK
                     DYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGNFYSIA
                     ASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
                     /name="hypothetical protein"
     gene            4714..5643
                     /locus_tag="LQT15_25395"
     CDS             4714..5643
                     /locus_tag="LQT15_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGE49752.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQP
                     HAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASASA
                     LKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRRELYTLDELADYLDLSASA
                     RRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVAVATSA
                     RTLKPWVTLGISRATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(5850..6296)
                     /locus_tag="LQT15_25400"
     CDS             complement(5850..6296)
                     /locus_tag="LQT15_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="UGE49753.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            6468..7552
                     /locus_tag="LQT15_25405"
                     /pseudo=""
     CDS             6468..7552
                     /locus_tag="LQT15_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     Typing          4715..5643
                     /name="rep_cluster_2141"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.613"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          753..1834
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.723"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6897..7549
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.083"
                     /coverage="100.0"
                     /evalue="1.97e-117"
ORIGIN
        1 gtgtttcgtt tggcaatggt tttagcgtct gtgtgtgagt ggtggttgtg ctcgccgcag
       61 tctcaatgac cgagcgtagc gagcgaatga gcgaggaagc accagggaac cggaacgtga
      121 acggaggctc ttacgcactg tctttggtga gtagctcttt cccgcgtatt ttcccattcc
      181 tgccattttg gatggttgcg cggtagctct gcgattcctg cgagtgcgcc taccagttcg
      241 gcagcactaa actttggttt ttcggttttg ctgtccacca gtcctgccac aatcatgagg
      301 cctgcggcct tgtagagttc atgatcccgc gcctttcgtt ccgcccttgc ggcctgcctt
      361 ccgttctgcc gctattattg ccgttaccac tggcttttgc tgcggctgct ttctctgcgg
      421 ttttttctgc ccgtatcagt gcggacaacg ttttttcatc cgtcgttgtt cggttttttt
      481 tctctgccag taacacaagt aatctctgct gctctgacgg tgatttcagt ccgttgatgt
      541 aggcaatttt tgcacttaac cactcttcac gatttgtcat tcttacctct gcacttttgc
      601 cgttaatgtg agttgttatt gtgcataata tcaccttgtc tgcctgcgtg tgagacctta
      661 aaatcatccc tgattaattc agggatgcgc acttagacgt ttctctcctt cgtcgagaaa
      721 cagtgcgctc ctgcggaggg cttaacggca gatggcaagc taccattgta cagttaaaac
      781 tggagcaaaa ggcgtagcct taaaacatgc tgattatatt tctagatccg gcgaatataa
      841 gaactacaaa tcgcgggagg atctggaatt ttcgtcatcc ggtaacatgc catcatgggc
      901 gaagaaaaat cccgcagagc tatggaaagc agcagatgaa tttgaacgaa aaaacgggac
      961 ggcataccgt gaaattgaga tcgcattacc gcgagaactg acacgcgaac aacggataga
     1021 acttgttgaa gatttcgtgc aaaaggagct tggtgatcgc catgcctatc agtacgcgat
     1081 ccacaatccg ccaggtgcaa tcgacggaaa ggaacaacca cacgcacata tcatgttttg
     1141 cgagcggatt aatgatggta ttgaacgcga tccgcagcag ttttttaaac gtgccaacag
     1201 caaatcaccg gagcggggag gtgcaaaaaa ggcacgcttt ggtgagacac cacaagagcg
     1261 aaaatcagcc ttggttgcgc tacgttctca ctgggctgat gtgcagaatg aacaccttgc
     1321 gcggcacggc catcagtcgc gcgttgatca tcgttcactg aaagaacagg gtatcaagcg
     1381 cacaccggaa gttcatcttg gtcccgttcg tgttgctgtt atgaatgctg atcaggtttg
     1441 tgcaattctg gcacggagaa acgcggaacg ggagaagcta gaagcagagc aggaacggga
     1501 cagcatcatt gaccttaaaa cgaccttgaa ggaagcgtta gttgaacggg ataacatacc
     1561 accaagcctg gatgagcttg gggcagaatt gaaggcgttt gttgaaagct cgtttgatga
     1621 actcaatgag gtaatgcagc ttaattcatt gattaacggt gcgctggact caatgatgaa
     1681 gcaattccag gtggaagagg agcggaagtt gcaacaggaa cgactaattg aagccgaaaa
     1741 gcaaagagac ctggagaggg aaagagaaag agtgcgccag cgtgcgtatt cctcaccagc
     1801 accatcacgt tcacgtgatt atggtatgag ccgttaagca ctggaggttt ttattttatg
     1861 caattggatg aacattcaag aaaaattgct gctcttgttg cgtataatga taaacaaata
     1921 aaagaaatga acaggctaat tactctgctt aatgaaagag tgaatgactt aaataccttt
     1981 acaagtactc ttgaacgtga aggtaaagtt gtttctggaa tttcttattc ctttagggat
     2041 agtgtgtcta atgttaaatt ttatattaat aaaggaatgg atgaggcatc ttgtaaatta
     2101 cagaaatcag tctcagatac actaacgtca ttactccgcg aaaaattatc tttcactctc
     2161 aatgatgtaa agaaagagtg ggttgatttc gaggagcaaa caagtaaaga gataaaggaa
     2221 tcattaaagc agacgaccag agaggccgaa gttgcgacag gtataattag aaaaacaatg
     2281 gagaagtata cagtaacaag gattgtcgaa aaggtaattg tgttgattgt attattttta
     2341 ttgctaatga tcgctcctgt tttctatgct gaatatctca aaatgaatat tagttcgcta
     2401 atggaagaac agaaaaaaca tgaatccatg ttaaaagaat ggaaagaaaa agctccattg
     2461 gccgagttct cgaagtgtgg agattatcgt aaagcatgcg tgttagttga tgacgaaagc
     2521 ggatattatt ttaatgaaca atcaccaaat aaaaaatatg ccatactgaa atagtgttaa
     2581 gtaactcatg ccgtcttatg actaaggctg catgagtata aaaacggcgt ttcacgtaaa
     2641 aataaaataa atacctgtac ctaatataaa aatggaacta catatgctta tgtatcttga
     2701 gaatgatggc gtcaatttat cccttatcgt taagctaata gctatgtaaa taagatctgt
     2761 taggaatgat ataaatataa ataaaacacc caatataaaa aagtttaatg tgatatttgt
     2821 attattatct ataaaggttg gtattaacgc agcaaagaaa agatacgcct tggggtttaa
     2881 taatggtatc aaaaaaggtt tttgctcttt tctttgtttg ttttttttat taattaaacc
     2941 cattaatcct atgtatatta aatagcctcc accgaatatt tttataatgt gaaaatgttg
     3001 ttctgtgctg gatgttgagg ataacgcgaa aagagatatt gccatgacga tagtctcgcc
     3061 aattcttatg tatatacttg tttttattga tgactttaat ccatgtaaaa aggcattgtt
     3121 tataaccaat gcaacgccag gccctattga tatagtttgc gataaggtta cgatagcaaa
     3181 tgataataat gttgaatatt ccatgtttac ttaccatgat atatttcgtg ggagcttttc
     3241 atatacatat tccataggaa ttgcagcttt cggtaataaa tttataaatg ttgatatgtg
     3301 ttttagtgaa ttgctggcgg cgattgagta aaaatttccg ctattccaat atattgatgc
     3361 gtatttaaaa tgttgtttaa ttgcggaaag catatcgtca acactaactt cattcaaatt
     3421 cattgtgttt agtacgattt cctttttggt gttaatctca tctttcgaga aatacctttg
     3481 tttgaatgaa taatcttttt ggatttctct tttttgatat tcatcatgtt ctgaactatg
     3541 tttttctgta aatatgaaaa taccatcatt atttaatagc ctttttacgt ggtttatttg
     3601 cccagatctg ttaggtgagt acatttgaaa tgtggtgtct tctattataa tatcgaatcc
     3661 ttgattaaat tgtttaagat ttgaattttt tagtctttcc tctaacagga aaaatggagt
     3721 gcaaataaaa taagcgtttt cacttgagcc atacttaaaa aatgcatgtt cattttcttt
     3781 tgttggactg gttgttaatg tttgtatgtt tccttttgat aattctgaaa tggttcttgc
     3841 cattgcgccc tcagccatac caagacacca tgtgtttaat tttttgttta gcgtgattgc
     3901 gtattttatt actattgttc ctaatctgca ctcttcttcc ttgctgtatg gtatactgca
     3961 aaaataatga ttaacgaaag ggccgctgtt ttttgtgagg tgtttggcaa acttaatgta
     4021 atcataagaa tgaaaaagcc gatcatctat ttctactagt ctaaaaggtt tgtgtttttc
     4081 ccctgatgca gttgatgaga aaaattctgc catgccgttt attctcggag cggaaataca
     4141 ttcatttata aaattattca ttttcctatc ctctggtttg atttttggaa tttgttgtta
     4201 tgactcttca atgctaattc tcttgtaagc ttttttacac gccataaaca cgattatgat
     4261 gttttatatt gtcgatattt cgttgtccag actggatatg tttggaaatt tgttttcatg
     4321 cgggttagtt cagctggctt tctggatgag aaagtttgct gttttgacca gttcgcgctt
     4381 ctgaaggggt gaaaccgatc attaatcgct tttgtgcata gttatgcagg cccctaaaaa
     4441 cgattctgac gcgttttttg tgttttccct aggtttatct tgtctttttg agttttttgc
     4501 gtcagaaagc gattgagagc gttttagagg gtacgtacaa gaggagttat ggtaaatggg
     4561 tctggttttc gggaaggatc gacaggattt gccgttgggt gtagtgtaac caactgaaaa
     4621 acaaacgccc ctgaaattcg tgccgatccg ccaagactgt cacgaaaatt caaggggcgt
     4681 gtgggtcgta acacacggag gaaatcctaa cacatgagcg ccgtacttca actcttcagg
     4741 gaaaaattac cgcacaaacc gtactgtacg aacgatttca cgtacggcgt tcgcattcta
     4801 ccgaaagata tcgccatcct tgcccgtttc atccagcaga accagccaca tgcgcagcac
     4861 tggctggcct ttgacgttga ccggacgggg gcagcgatcg actggagcga ccggaactgt
     4921 ccggccccga acatcaccgt aaaaaatcca cgtaacgggc acgcgcatct gctctacgcg
     4981 ctcactcttc cggtgagaac cgcgccggat gcatcggctt cggcgctcaa atacgctgcc
     5041 gtgattgagc gtgcgctgtg tgaaaaactg ggcgcggatg tgaactacag cggcctgatc
     5101 tgcaaaaatc cgttccacca ggaatggcag gtggtggaat ggcgtcggga actctatacc
     5161 ctcgacgaac tggcggatta tcttgatttg agcgcctcag cgcgccgtcg cgtcgataaa
     5221 gattacggga tggggcgaaa ctggtatctg ttcgaaaagg gccgtaaatg gtcttacagg
     5281 gcgattcgtc agggctggcc agcattctca ccgtggcttg atgcagtgat tcagcgtgtc
     5341 gaaatgtaca acgcatcgct tcccgttccg ctttctccgg ctgaatgtcg ggctattggc
     5401 aagagtattg cgaaatacac ctaccgcaaa ttctctcctg aaggcttttc cgcggtacag
     5461 gctgctcgag gtcgtaaggg cggtaatgca aattcatcag aaaatcaggc tattaaaggt
     5521 cagaaatcaa agcgtgtagc cgttgccaca tcggcacgaa ctctgaagcc gtgggtgacg
     5581 ttagggatca gtcgtgccac atactatcga aaactaaaat gtgatccaga tctcgcaaaa
     5641 tgagaccaga taacaagcct tatcagataa cagcgcctgt aggcgttttt ttgagcgggt
     5701 aggtttgggg tgatttcccg tgtttcgttt ggcaatggtt ttagcgtctg tgtgtgagtg
     5761 gtggttgtgc tcgccgcagt ctcaatgacc gagcgtagcg agcgaatgag cgaggaagca
     5821 ccagggaacc ggaacgtgaa cggaggctct tacgcactgt ctttggtgag tagctctttc
     5881 ccgcgttttt cccattcctg ccattttgga tggttgcgcg gtagctctgc gattcctgcg
     5941 agtgcgccta ccagttcggc agcactaaac tttggttttc cggttttgct gtccaccagt
     6001 cctgccacaa tcatgaggcc tgcggccttg tagagttcat gatcccgcgc ctttcgttcc
     6061 gcccttgcgg ctgccttccg ttctgccgct attattgccg ttaccctggc ttttgctgcg
     6121 gctgctttct ctgcggtttt ttctgcccgt atcagtgcgg acaacgtttt ttcatccgtc
     6181 gttgttcggt tttttttctc tgccagtaac acaagtaatc tctgctgctc tgacggtgat
     6241 ttcagtccgt tgatgtaggc aatttttgca cttaaccact cttcacgatt tgtcattctt
     6301 acctctgcac ttttgccgtt aatgtgagtt gttattgtgc ataatatcac cttgtctgcc
     6361 tgcgtgtgag accttaaaat catccctgat taattcaggg atgcgcactt agacgtttct
     6421 ctccttcgtc gagaaacagt gcgctcctgc ggagggctta acggcagatg gcaagctacc
     6481 attgtacagt taaaactgga gcaaaaggcg tagccttaaa acatgctgat tatatttcta
     6541 gatccggcga atataagaac tacaaatcgc gggaggatct ggaattttcg tcatccggta
     6601 acatgccatc atgggcgaag aaaaatcccg cagagctatg gaaagcagca gatgaatttg
     6661 aacgaaaaaa cgggacggca taccgtgaaa ttgagatcgc attaccgcga gaactgacac
     6721 gcgaacaacg gatagaactt gttgaagatt tcgtgcaaaa ggagcttggt gatcgccatg
     6781 cctatcagta cgcgatccac aatccgccag gtgcaatcga cggaaaggaa caaccacacg
     6841 cacatatcat gttttgcgag cggattaatg atggtattga acgcgatccg cagcagtttt
     6901 taaacgtgcc aacagcaaat caccggagcg gggaggtgca aaaaaggcac gctttggtga
     6961 gacaccacaa gagcgaaaat cagccttggt tgcgctacgt tctcactggg ctgatgtgca
     7021 gaatgaacac cttgcgcggc acggccatca gtcgcgcgtt gatcatcgtt cactgaaaga
     7081 acagggtatc aagcgcacac cggaagttca tcttggtccc gttcgtgttg ctgttatgaa
     7141 tgctgatcag gtttgtgcaa ttctggcacg gagaaacgcg gaacgggaga agctagaagc
     7201 agagcaggaa cgggacagca tcattgacct taaaacgacc ttgaaggaag cgttagttga
     7261 acgggataac ataccaccaa gcctggatga gcttggggca gaattgaagg cgtttgttga
     7321 aagctcgttt gatgaactca atgaggtaat gcagcttaat tcattgatta acggtgcgct
     7381 ggactcaatg atgaagcaat tccaggtgga agaggagcgg aagttgcaac aggaacgact
     7441 aattgaagcc gaaaagcaaa gagacctgga gagggaaaga gaaagagtgc gccagcgtgc
     7501 gtattcctca ccagcaccat cacgttcacg tgattatggt atgagccgtt aagcactgga
     7561 ggtttttatt ttatgcaatt ggatgaacat tcaagaaaaa ttgctgctct tgttgcgtat
     7621 aatgataaac aaataaaaga aatgaacagg ctaattactc tgcttaatga aagagtgaat
     7681 gacttaaata cctttacaag tactcttgaa cgtgaaggta aagttgtttc tggaatttct
     7741 tattccttta gggatagtgt gtctaatgtt aaattttata ttaataaagg aatggatgag
     7801 gcatcttgta aattacagaa atcagtctca gatacactaa cgtcattact ccgcgaaaaa
     7861 ttatctttca ctctcaatga tgtaaagaaa gagtgggttg atttcgagga gcaaacaagt
     7921 aaagagataa aggaatcatt aaagcagacg accagagagg ccgaagttgc gacaggtata
     7981 attagaaaaa caatggagaa gtatacagta acaaggattg tcgaaaaggt aattgtgttg
     8041 attgtattat ttttattgct aatgatcgct cctgttttct atgctgaata tctcaaaatg
     8101 aatattagtt cgctaatgga agaacagaaa aaacatgaat ccatgttaaa agaatggaaa
     8161 gaaaaagctc cattggccga gttctcgaag tgtggagatt
//
