LOCUS       CP088446               11126 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0184 plasmid pF16EC0184-3, complete
            sequence.
ACCESSION   CP088446
VERSION     CP088446.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391679
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:34:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,086
            CDSs (total)                      :: 4,967
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 78 of 207
            Pseudo Genes (incomplete)         :: 141 of 207
            Pseudo Genes (internal stop)      :: 36 of 207
            Pseudo Genes (multiple problems)  :: 41 of 207
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0184"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0184-3"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(11045..11126,1..110))
                     /locus_tag="LQT15_25285"
     CDS             complement(join(11045..11126,1..110))
                     /locus_tag="LQT15_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UGE49743.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            178..501
                     /locus_tag="LQT15_25290"
     CDS             178..501
                     /locus_tag="LQT15_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UGE49744.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            710..1147
                     /locus_tag="LQT15_25295"
                     /pseudo=""
     CDS             710..1147
                     /locus_tag="LQT15_25295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1179..1739
                     /locus_tag="LQT15_25300"
     CDS             1179..1739
                     /locus_tag="LQT15_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UGE49745.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1746..1979
                     /locus_tag="LQT15_25305"
     CDS             1746..1979
                     /locus_tag="LQT15_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UGE49746.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2463..2885)
                     /locus_tag="LQT15_25310"
     CDS             complement(2463..2885)
                     /locus_tag="LQT15_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UGE49747.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3007..3285
                     /locus_tag="LQT15_25315"
     CDS             3007..3285
                     /locus_tag="LQT15_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49748.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3277..3567)
                     /locus_tag="LQT15_25320"
     CDS             complement(3277..3567)
                     /locus_tag="LQT15_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE49749.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3808..5454
                     /gene="cka"
                     /locus_tag="LQT15_25325"
     CDS             3808..5454
                     /gene="cka"
                     /locus_tag="LQT15_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="UGE49750.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEDNSTLVDNPFG
                     GVSRVLNSLISYMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFITLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(5451..5741)
                     /gene="cki"
                     /locus_tag="LQT15_25330"
     CDS             complement(5451..5741)
                     /gene="cki"
                     /locus_tag="LQT15_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="UGE49751.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(5741..6763)
                     /gene="lamB"
                     /locus_tag="LQT15_25335"
                     /pseudo=""
     CDS             complement(5741..6763)
                     /gene="lamB"
                     /locus_tag="LQT15_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709883.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /name="lamB"
     gene            complement(6812..7219)
                     /locus_tag="LQT15_25340"
                     /pseudo=""
     CDS             complement(6812..7219)
                     /locus_tag="LQT15_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491235.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            complement(7299..8174)
                     /locus_tag="LQT15_25345"
     CDS             complement(7299..8174)
                     /locus_tag="LQT15_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-14"
                     /protein_id="UGE49739.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSSG
                     GRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKPAD
                     LVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGDETF
                     RLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAA
                     SIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESRRDVLA
                     SAARIIAEGL"
                     /name="class A extended-spectrum beta-lactamase CTX-M-14"
     gene            complement(8424..9686)
                     /locus_tag="LQT15_25350"
     CDS             complement(8424..9686)
                     /locus_tag="LQT15_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="UGE49740.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDST
                     NKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTT
                     QMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQF
                     AFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIET
                     IESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRV
                     LKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGH
                     LLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTAR
                     YVIMKLSENYPYKGVYEKCLV"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            9908..10039
                     /locus_tag="LQT15_25355"
     CDS             9908..10039
                     /locus_tag="LQT15_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UGE49741.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10234..10377)
                     /locus_tag="LQT15_25360"
     CDS             complement(10234..10377)
                     /locus_tag="LQT15_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGE49742.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     ARG             complement(7303..8174)
                     /name="blaCTX-M-14"
                     /gene="blaCTX-M-14"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-14"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          10873..10960
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(10306..10862)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          492..2086
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcagcct ggtcgtcggc gtccagttca ttcagttttt ccagcagggt cagcgtctgg
       61 cttctgatga agcgggccat gttaagggcg gttttttcct gtttagtcac agttctgttt
      121 ctccatgtgt ggggagtctg tcggaggttt ttaatgatac cggtgaaacg cgaaaggatg
      181 ctcacgatac gggtcacgga tgaggaacat gcgcggctgc tggcacgttg tgagggaaaa
      241 caacttgcgt catggatgcg taaggtctgc ctgggggcac cgccatcgaa aacgtcaggt
      301 cttccgacac tggcaccgcc gctgttgcgc cagttcgctt cggtcggcaa taacctgaat
      361 cagatagccc ggaagataaa cagcggacag tggtcggggc atgaccgggt tcatgtggtg
      421 gctgcactga tggccatcgg acgtgaactg agtgagctga gggatgaggt ccgtaaacag
      481 ggagaacggg atgatagtta agtttcatgc caggggaaaa ggcggtggca gtggtccggt
      541 tgattacctg ctgggccggg agcgtaaccg ggaaggggcg agagttctgc ggggtgctcc
      601 cgaagaggtg cgggaactca tcgatgccac gccgtttgcg aaaaaataca cctcgggggt
      661 tctgtctttt gcagagcaga ccctgccacc gggagagcgt gaaagggtga tggagagctt
      721 tgagcgggta ctgatgcccg gtcttgaaaa aaatcagtac agcatcctgt gggtggagca
      781 ccaggacaag gggcgtcttg agctgaattt tgtcatcccg aatatggagc tggcgagcgg
      841 aaaacggctc cagccctatt acgaccgagc ggaccgtccg cgaatcaatg cctggcagac
      901 actggtgaat caccattacg ggctgcacga cccgaacgca ccggagaaca ggcggacact
      961 tgtcaccccg aataatctgc cgaaagcgaa acaggaggcc gcagaagcga ttacgagggg
     1021 actggaggct ctttaccatg ccggagcgct aaaaacgcgt caggatgtca ctgaggcgct
     1081 tacagcggca ggttttgagg ttgtgaggac aaccaggagc agtatcagta tcgccgaccc
     1141 ggagggtggg cgtaatctca ggctgaaagg ggcgctgtat gagcaatctt ttacaaatgg
     1201 ggacagactt cgagaagaaa ctgaaagagc gtgccgtctc tacagagaac atgctgaaca
     1261 gcgagttcag gaagctcgaa gcgtctgtag acagggaact gaacttaaac gggcagaaaa
     1321 tcagagacgc tatcagcgca cacacagcag cggtgaaaga acaactggag acgctgaaca
     1381 gtgccgtgaa cgcgcagttc agcgccacag agaacgaact ggcccggcgg caggaagagc
     1441 tgctctggaa gctggtcaaa gggcggatac tgtacccgtc gctgacggcg ctttgcgtca
     1501 cgggcgggat ttttctggcg tcatgggggc tgattcagtg gcaggaaagc cggattgcgg
     1561 cgaacatcct ggctatcagg gaccaggaag agacgctggc gaaactccgg gaaaaaacat
     1621 ggggtgtgac gtatcacgag ggcaggaacg ggaaatttct ggtgttgccg tccggggtga
     1681 agggggaaaa caactggacg gtggagaaga agaacgcagt aagactggtc agggagtaag
     1741 tgagtatgac agagctggaa atgcacttac tgaacgcctt agagcaattg cagcaggact
     1801 atacgcagcg gctgaaagac tgggagagcg ccttcacgga tttgcagcat atgttcgagc
     1861 tgacacgaca ggagaacggg caactgaggg aacagtgcga cgacttgagt cagcaggtgc
     1921 agagattagc cgggcaggtg gaccacttga gtcggttatt tatcacgaac agcagataag
     1981 ggatgaacag gaaaggcagg cacgcctgga acgggagcgt gcgcggtcct ggcatgaaga
     2041 gcaggaacgg gaagcacggg tatggcgtgg cccgtcaata gggatgtgac ggtgagttgt
     2101 tccgggaggt tgccagatgg taccggaacg cgttaaaaac gcatccgggc gcgattctgg
     2161 cgtgtcgttt ttgttgctgt gttttgcctc tttgccagat cgcgctcatt cctcagcctc
     2221 atcccgcgat gtctgcgtat gtcatcaact gaaaagatgg aaaaaaaaca gtgtcatgag
     2281 gtttacgcag gcaaaaccag cgttattcac atggctgaat agctatgcat acggttaaaa
     2341 tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt tgccattttt acctgtcagc
     2401 agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa ttaagggatt atggtaaatc
     2461 ccttactgtc tgccctcgta tccatcgaga taaaccgcag aaatggtgtc tgccattagt
     2521 ttcatttttt tcggcgaagg catatcgctg tttgtgtatt ttctgccagc aaaggctgtt
     2581 gtgctccgga gtgagtgaaa aaatttttct gagtggaatt cttttacacg ttgctgtgcc
     2641 gtttctgctg atactccggc tgctttgtct ttcatcccca tatcccggaa ctgctgaaag
     2701 aacgggagca tcatacgtat ggtgtctgct ttctgggtgt agaagttagg atcaaatcca
     2761 tcatctgaag cataaacata atgccgggta tgtcgttcca ggctggtctt tggtgtgcat
     2821 cctgtaagag ctattgttgc tgttatcaga ggtatcagaa gcagcctggc agcggcagat
     2881 ctcataaaca tctccttgcg tttttaatct tgatactcag ggagttatat ctccgaagag
     2941 tatctcttat catacgtcgg tttactccca atgtgatttg atggtcaatg agtcaggaat
     3001 aatcccgtga aaaaatgtgt gccagtcctg ctttttagtg ttctttctgc ttcggttgcc
     3061 agtcatgcgg acagcaatgt ttttcaacaa aaggccgaga agacaaaaaa cggcattgtt
     3121 gcggatgtta atgccagaca gaaaatgttc agtaacagac tgaagataca acttcagatc
     3181 gatgagttga gagcagagct ggctggcacc agagatgccc aaaaaagaga ggatttgcgt
     3241 aaaaaactgg agtctctgga gcggcaaaag aaatccttac gctgatgggg gcgtattttc
     3301 cccggtttct tccataacct ttttaccgtt cagatattta cgggcgaggc gaatgcgtct
     3361 tgcctggtat accgccgagt aagcctgggt gggattcaca ccattactga tttcactgac
     3421 cacatgacgc tgatcagcca gtgtcagcgt gtaaacctgt ttctggatga ggtcacgggc
     3481 tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga agtcgtttgt ctgttttgtt
     3541 cagtttatgg cggggtgatt tgctcacatc gttctccgtt ttttcatgat ccaacagttt
     3601 tatatctcat cctgatttgc tccggtgttc tgtttcgacg ttctcaatgc ctgcctcccc
     3661 atagtgtgat ccttattgca aaaatgaaaa tccatgctct tgacatggac aatgctgagt
     3721 agtaggtttt tactgtacat aaaaccagtg gttatatgta cagtatattg tttttaattt
     3781 attgttttaa ttatcaaaga ggaatttatg gctaaagaac taagtggata tggaccaact
     3841 gctggtgagt ctatgggggg aacaggggcg aatctgaatc agcaaggtgg aaataataat
     3901 agtaatagtg gggttcactg gggtggtggc tctgggcatg gtaataatgg tggtcaggga
     3961 aattcaaatt catctggttc tacatcaact gtgatgaaaa ccggtgagtc atatctgact
     4021 ccgtggggtg atgttgtcat taataatgat ggccttccgg tgatgaatgg tattgttatg
     4081 acagaagata attcaactct ggttgataat ccatttggag gggtctccag agttcttaat
     4141 tctttaatta gttatatgcc atcacttttt gcagaaagct caggaaataa caacaataac
     4201 actgcttcag ttaatactgc accaacgaat gctcaggtca gtgatatgga taagagttcg
     4261 aaggtggtgg gtaatgtaat taatgaaaaa caaaagcaga agaataaaat tgctacacag
     4321 attagtgaaa aacagaagaa aatcgaggag atgaaaaaag tattcaagca tcattcttat
     4381 catggtatca ctgatcttga gagggatgtt gatgagttgc aaaaaaaatc aaatcagctt
     4441 gatgcagata ttagcaaact taattcttat aaaaatacat tgcaaagtaa aattggtgat
     4501 gtgaataaac agaaggaagc tgaagaaaag gctcgtgaaa atgcagaggt cgctgaacat
     4561 gaaactctta atgaagagaa acaggcggtg gcggaagcag agaagcgttt agctgaggcg
     4621 aaagctgaac tggcgaaggc tgaaagtgat gtacagagta agcaagctac tgtttccaga
     4681 gttgcaggag agcttgaaaa cgctcagaag agtgttgacg tgaaggtcac cggatttccg
     4741 ggatggcgtg acgttcagaa aaaactacag cgtcagcttg aagctaagca ggctgagtac
     4801 tccgcagttg aaaatgaact taagaatgct gttagtttca gggatggtaa ggccgctgag
     4861 gttaaagagg cagagcagaa attaaaagaa gctcaggatg ctcttgaaaa gagccagata
     4921 aaagatgctg ttgatactat ggttggattt tatcaatata taaccgaaca atatggagaa
     4981 aaatacgcaa aaattgctca ggatttagcg gaaaagtcta aaggaaagaa aattcaaggt
     5041 gttgatgaag ctctggcagc atttgaaaag tataagaatg tactggataa gaaattcagt
     5101 aaggttgatc gggatgctat ttttaatgcg ttagagtctg ttaattatga tgagttatct
     5161 aagaatttaa cgaagatatc aaagtcgctt aaaatcacca gtcgagtatc ttttctatat
     5221 gatgttggtt ctgattttaa aaatgcaata gaaactggta actggcgacc tttgtttatc
     5281 acgctggaga aaagtgcggt cgatgtaggt gtggcaaaaa ttgtggcatt aatgtttagt
     5341 tttattgttg gtgtgcctct tggtttctgg gggattgcaa ttgtcacagg tattgtttct
     5401 tcttacatag gggatgatga gttgagcaaa cttaatgagt tgttagggat ttaatttctc
     5461 tttagagtgt aatatatgaa acttggcaat cctactggga ttgccattaa taaataaaac
     5521 aatgcagata acttgcataa gtaccagggt tcttttttta actttgtatt ttctttgata
     5581 tatctagata tggttaattt agagtatgga tatagcaata catgtattga tgatataaat
     5641 ataaataaca aaggagtgtt atcattgtcg ttaaatatta aaataagaat ccatggtata
     5701 agtgattcag gtaaattatg taggtagtat tttaaatgca ctgggattcg acgttgtcgt
     5761 agccgatttc catcacggtg ctcatgattg gcgtccactt gtacatcggg cgaataccga
     5821 cggtccacca cttggtgccg ttgtcgttat cccagttgat atcctggtac atacccacgt
     5881 acatcatgtc ccagttgtcg cccatggaga tcgctccgtg gtcgaggata cgcagcatgt
     5941 gaccgttgtt gttgatattg taggcaaatt tttcgttatc aaacgcgacg ccagaaccct
     6001 gcgacagacc tttaccctgc gaggtcatcg agtcagtagc gtactgaaca acaaacttgt
     6061 taaagccctt caggacactc tgagtatgtt cagcagtgaa caaccagccg tctttcgatg
     6121 cgccatcaac cagacgatag ttatcacgca ggttggcacg accgtagtcg acacccagtt
     6181 ctaatgtgcc gcccggattg atttccatct gcgctaaacg cacatcgaaa acgtcgttcg
     6241 cggtttcgtt ggtatagtca taaatattgt tgctggcgaa agaggaagaa ccacctgctt
     6301 cagaggagcg ggttgctgcc agagagagtt taccgaagcc aacatcgatg ttttccagac
     6361 cggcaccagg accagaaata tcccagtagt agaagtcgat catatgaacg tcatgacgtt
     6421 ggtagaagcg cttacctgcc cagatggtgg agcctggcag ccattcgatc aggtttttac
     6481 cctgcacgtt tgcttcacgg aaggccggat cggtagcttc ccagtcattc tgttgtgcga
     6541 cggaataggc cacgttagtg tcgaaataga agctcttatc gccctctttc cacacttcct
     6601 gacccaattt taattcagca taagtttcac attcgttgcc aagacggtat ttactttgag
     6661 caccggtagt ctggaaacac tgttgttcac cgccgctacc tgtccagcca ataccggaac
     6721 gtgcatagcc gtggaaatca acagccattg cctgagcaga cattacgccc gctgcgacgg
     6781 caaccgccag aggaagtttg cgcagagtaa tcttgacttt ccactcgcct tcaccgaaga
     6841 ccttcagccc ggtggaatca attaccaggt gtgcgatttc accccgggtg ggcgttttga
     6901 aactgatatt aaccgacttt gcccgcctgc tgacacagct gtaatccggg cagcgtagcg
     6961 gaacgttcat cagagaaaaa atggaatcaa taaagccctg cgcagcgcgc agggtcagcc
     7021 tgaatacgcg tttaatgacc agcacagtcg tgatggcaag gtcagaatag cgctgaggtc
     7081 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatagcttca tcatccagcc
     7141 agaaagttat ggagccacgg ttgatgaggg ctttattgta ggtgggccag ttggtgattt
     7201 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatacgtg atctgatcct
     7261 tcaactcagc aaaagttcga tttattcaac aaaaccagtt acagcccttc ggcgatgatt
     7321 ctcgccgctg aagccagcac atcgcggcgg ctctctgcgt tctgttgcgg ctgggtaaaa
     7381 taggtcacca gaaccagcgg cgcacgaccc tgcggccaga tcaccgcaat atcattggtg
     7441 gtgccgtagt cgccgctgcc ggtcttatca cccacagtcc acgacgtcgg taagccggcc
     7501 cgaatgctgg ctgcgccggt cgtattgcct ttgagccacg tcaccaactg cgcccgctgg
     7561 gtttcgccca gcgcatgacc cagcgtaagc tgacgcaacg tctgcgccat cgcccgcggc
     7621 gtggtggtgt ctctcgggtc gccgggaatg gcggtattca gcgtaggttc agtgcgatcc
     7681 agacgaaacg tctcatcgcc gatcgcgcgg gcaaaagccg tcacgcctcc cgggccaccg
     7741 agctgggcaa tcaatttgtt catggcggta ttgtcgctgt actgcaacgc ggccgcgctc
     7801 agttctgcca gcgtcattgt gccgttgacg tgtttttcgg caatcggatt gtagttaacc
     7861 agatcggcag gcttgatctc gacaggctga ttaagcagct gcttttgcgt ttcactctgc
     7921 ttaagcaccg ccgcggccgc cataacttta ctggtactgc acattggaaa gcgttcatca
     7981 ccgcgataaa gcacctgcgt attatctgcg gtatcgatga gcgcgacgcc cagccgccct
     8041 ccgctgcttt tctccagcgc cgccagcttt tgctgcaccg cactcgtctg cgcataaagc
     8101 ggcgcgctgc ccagcagcag cggaatgcac gccgccgccg cgaacatcat ccgttgcact
     8161 ctctttgtca ccatctcaaa ctcccaatac ggtcaatccg tgttacatca gtattcccta
     8221 aattccacgt gtgtttttta ttagcttcaa aaatcactat ttcacgaaga atttagactg
     8281 cttctcacac attgtaacat tatttacaac cacctttcaa tcatttttga taaatcattg
     8341 atttcatctt tgctgcaatg atacttaata aactctgcaa gttatccaca gagcaacact
     8401 caattttatt gatgatattc ttattatacc agacattttt catacactcc cttgtacgga
     8461 tagttttccg acaacttcat gattacatat cttgcggttt tgattatttt tgctgcaaga
     8521 aatacatact tcaaacgaaa ggtctttatt tgctgtctgt attctgaaga gtccaaggaa
     8581 tcaaacttga acaacaaaaa taggttatat gaaagcatca tcatttgaaa cacggcttca
     8641 ttcgcccaaa atgactttag caagagatga cccaccgcca tgtcgtattt ggcttctttg
     8701 atatagtttt cagcattacc acgcttttca tagtatataa ctactttttc agaaagcaag
     8761 gtagtatttg ttacaaagaa aaagtagtcg tattcggaac cttctaaaag tgataattgt
     8821 gctctttctt tttctggttt cagtacgcga gatacgacaa atcttctgtc tttttcccat
     8881 ttaactaatt ttgtatacag ttctgtagtt tctctacctt cttctccttt aacgaataca
     8941 attgatgaat tcgttgcttg tgaggtgagt gtagaataac ttttggcttt aattaaatat
     9001 ttgcatccaa gagattctat cgtttcgata attttttcat caaagtagcc actatccatt
     9061 cgaaataaaa tttctaaatc gtctgatttg atgttagcaa caatttcttt gatcatttcc
     9121 gcagcaccgt ttgcagtgta agtattgcca cttcttacaa atccggtaac atatgctttt
     9181 aattcgtcgc aaaatgcaaa ttggatattg tagcatcggt ttcccagttt cttaggatta
     9241 tatccttttg acgcaccttc ttgatgacct tctacgttaa ttacactact atcaatatca
     9301 atcgtaatgg atgtcaattt acttttagtg agcagttttt taaagacttt aaaattaatg
     9361 tctctaaaca tttgggttgt cttgaagttg aagtttccta gaaaccgtga cactgtttca
     9421 ggttctttta cggaaatatc aaactcgttg acgaggggat cattttgaag tagctttaga
     9481 cgttctaact tatcaatgcc aatgaagtga ccgcagagca tggtctttat atgattcatc
     9541 ttgattttat ttgttgagtc attatcaaat acgaggtcat tttcaataaa atcaaaaatc
     9601 ccattgcttt ttgcattctc aaggagcaga aaaagacctg catttgatgt tagattctta
     9661 gctttgaaat caattttatt aatcataatt agaacccctt tttactactt ttcttactat
     9721 tattttacca tatatcgagt cataaaagct gataatttaa catatttttg agcacttttc
     9781 tttcacccaa tgggtgaaag ctgaatttcg aaggaatgca tatttatcaa ggctttgatt
     9841 atgctttttg aagtactgac gtagaatcta ggatgcataa gtatttttac ttttgttgga
     9901 aagaaatatg aaaataataa tttttctaat tacaatatta ttcttgtcag gatgtcaagc
     9961 aaactatatt cgtgatgtac aaggaggaac cgttgcacca tcttcgtctt ctgaactgac
    10021 ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10081 gtaatctgct gcctgtaaac gaaaaaaccg cctggggagg cggtttagtc gaaggttaag
    10141 tcagttgggg actgcttaac cgggtaactg gctttagtgg agcacagata ccaaatgctg
    10201 tccttccagt gtagccgcta taaggcttca acttcaagag ttctgcgtat ctaaatccac
    10261 tatccagtta ccaatggctg ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc
    10321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10381 gcccagcttg gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga
    10441 aagcgccacg cttcccaaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10501 aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt aagtcctgtc
    10561 gggtttcgcc acctctgact tgagcgtcgg tttttgtgat gctcgtcagg ggggcggagc
    10621 ctatggaaaa acgccagctg cgtggccttt tccgggttct tgttttttgc tcacatgttt
    10681 cttttggtgt tatcccctga ttctgtggat aaccgcatta ccgtctttga gtgagctgat
    10741 accgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga agcggaaaag
    10801 cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggtgc
    10861 acttttcata caatcctcac tgatgccaaa tgattaagcc agtatacact ccgctatcgc
    10921 tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac gcgccctgac
    10981 gggcttgtct gctcccggca tccgctcaca ggccagctgt gaccgtctcc gggagctgca
    11041 tgtgtcagag gttttcaccg tcatctccga agcgtgcgag gcagctgcgg taaagctcgt
    11101 cagcgtggtc gtgaagcgct tcgcag
//
