LOCUS       CP088441                5631 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0186 plasmid pF16EC0186-5, complete
            sequence.
ACCESSION   CP088441
VERSION     CP088441.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391680
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 129x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:35:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,393
            CDSs (total)                      :: 5,280
            Genes (coding)                    :: 5,063
            CDSs (with protein)               :: 5,063
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 78 of 217
            Pseudo Genes (incomplete)         :: 160 of 217
            Pseudo Genes (internal stop)      :: 40 of 217
            Pseudo Genes (multiple problems)  :: 51 of 217
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0186"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0186-5"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(5547..5631,1..446)
                     /locus_tag="LQR13_26275"
     CDS             join(5547..5631,1..446)
                     /locus_tag="LQR13_26275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UGE40113.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            453..686
                     /locus_tag="LQR13_26280"
     CDS             453..686
                     /locus_tag="LQR13_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UGE40114.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1167..2219
                     /locus_tag="LQR13_26285"
     CDS             1167..2219
                     /locus_tag="LQR13_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGE40115.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSIIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2221..3087
                     /locus_tag="LQR13_26290"
     CDS             2221..3087
                     /locus_tag="LQR13_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="UGE40116.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4290..4481)
                     /locus_tag="LQR13_26295"
     CDS             complement(4290..4481)
                     /locus_tag="LQR13_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UGE40111.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4513..4869
                     /locus_tag="LQR13_26300"
     CDS             4513..4869
                     /locus_tag="LQR13_26300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UGE40112.1"
                     /translation="MGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGE
                     PVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRR
                     LRLAVREQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4115..4196
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          3471..3658
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          3..790
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.68e-148"
     Typing          4860..5629
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.82e-169"
ORIGIN
        1 agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa tctttaatca
       61 ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg tatcagaaaa
      121 cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc gtgaaacgct
      181 ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt ctgctgtggt
      241 accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa gagagtctgg
      301 agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac ggacgttttc
      361 tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac ggcaaactga
      421 acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga catttgctga
      481 acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg gagaacgcct
      541 tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg ctgaacgggc
      601 gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa cgcttgagcc
      661 agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac gcgaacatga
      721 gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg atggcccttc
      781 gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg gctaatgtgg
      841 cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt gcgatcgcgg
      901 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcaccgaacg
      961 cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt caggaaggag
     1021 tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc ggtaacgata
     1081 ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat ataaaaaaat
     1141 atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat aaactaccac
     1201 aattatcatc cagtttttca tttggaaaag atattgataa tattcattca attatcttta
     1261 acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca ggcaatcagc
     1321 cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc catttatcta
     1381 ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt ttgcgtaatg
     1441 aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat ctcatttatt
     1501 ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac atacaaaaat
     1561 atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt atttatactg
     1621 aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt gttaatgttt
     1681 tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg ggaatactta
     1741 tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt ttctataaag
     1801 atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt ggtaagggtg
     1861 ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat ttggatcgtt
     1921 ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat catactgatg
     1981 tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata gaaaatagtg
     2041 atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag tttcctatag
     2101 atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct atgtttttta
     2161 actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag gatgattaat
     2221 atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc agctatcgta
     2281 atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg gcagatttgt
     2341 agtgcagtaa tactatcttg gatagttctt tttataaaaa gaaaaaaact cccattaaac
     2401 tcaaaagcga tacttgctta tgcgacaggc gtttttgaac cattcttagc gtatactttt
     2461 acactctacg gactttattt tatttcagca ggaagcgcat caattatttt ttcacttgaa
     2521 tcagtgttta tattggtgtt gtctgttctt atttgctcag ttaaaattaa ttccccgcaa
     2581 tattttatat tgttccttat aggtgcaatt gttggcagta ttttggtagc cttgccagac
     2641 atcagtaatg gatcgaataa tattgttgga tattgtcttg ttgttgccgg tgtgttatcg
     2701 gcagcatttt atgttgttat atcgtctcgc cttattaaat cgtttgagcc tatcacatta
     2761 ttgagcggac agttgacatt ctcccttata ctgtcatcgc tctttatttt tatatctaaa
     2821 tcatcaattt atcttccgtt ggaatccgtg attattgttt tttcatctgg aatattacaa
     2881 tattttttag ctttttgttt ttacttgaac tccttgcgtt ggctcccagt tcatttggca
     2941 gggataatgt tgtactttat tccgctagtt gcattatttt tgtcatggtt gtttttggat
     3001 gaacaattca cattcttaca gggggtagga tttattgtaa ctgtgctaag tgtatatttt
     3061 cttaacagaa aatactcaga ggagtaacat tctgttcagg attgcccccc ggcctccatc
     3121 gataccgtgc caaatgcacg taaaaaacgg gagcgtttct ggtgtcggaa tgtaaccaag
     3181 ggggtcagag gcgcgctaaa acgctctcag gggagtcaaa tagagacgtt tgtaacaaaa
     3241 agatcaaagg atcctcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga
     3301 aaaaaccgcc ttgcagggcg gtttgatcga aggttaagtc agcttgcaga ctgcttaacc
     3361 aggtaactgt cttatgcgag acgagtcacc aaaattgttc ttctagtgta gccgcactcc
     3421 ggcagccact tcaagtactc tctaaatatc tcgcacatga cttttaccaa tggctgctgc
     3481 cagtggcgct ttgtggtgtc ttttccgggt tggacccaag agaacagtta ccggagaagg
     3541 cgcagcagtc gggctgaacg gggggttcct gcatacagcc cagcccggag cgaactgcct
     3601 aagtcgcccc gagataccag aagacgtgag ctatgagaaa gcgccacgtg tcccgtaagg
     3661 gagaaaggcg gaaaaggtat ccggtaatcg atatggcggg aacgtggggg cgcaggaggg
     3721 agcgattcac cggaaacggt gatgatcttt aagccacgtc gggtttcgcc accgctggcg
     3781 gatttcatgg ttgagccgca aggctcccac agatgcaccg aaaaagtgtc tgttttgtga
     3841 agtccgtcag gggggcggag cctatggaaa aagcgcctcc ggcgcggcct ttcccggggt
     3901 ttcgtttttt gcgcacatgt tctttcctgg tttatccccg ggttctgtgg ataacagggt
     3961 tagcgttttt gagaaaggag agtccgctcg ccgcagccga acgaccgagc gtagcgagtc
     4021 agtgagcgag gaagcggaag agcgcctgaa ctggcatttt ctccttacgc atctgtgcgg
     4081 catttcacac cggacatggc gcacttttca tacaatccgc actgatgccg aatggttaag
     4141 ccagtataca ctccgctatc gctacgtgac tgggtcaggg cttcgccccg acaccccaaa
     4201 ggtcaacggc ctgacgcgcc ctggcgggct tgtctgctcc cggcatccgc tcacagacaa
     4261 gctgtgaccg tctccgggag ctgcatgtgt cagcggtttt caccgtcatc cccgaagcgt
     4321 gcgaggcaac tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca gatgtctgcc
     4381 tgttcatccg cgtccagttc gttgagcttc tccagcagcg ttaatgtctg gcctctgatg
     4441 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     4501 ggggttctgt tcatgggggt aatgataccg atgaaacgcg agaggatgct cacgatacgg
     4561 gttactgatg atgaacatgc ccggttactg gaacgctgtg agggtaaaca actggcggta
     4621 tggatgcggc gggtctgcct gggtgagccg gttgcccgtt ccgggaagct gccgacactg
     4681 gcaccgccgt tactgcgtca gctggccgcc atcgggaata accttaacca gacagcgagg
     4741 aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg
     4801 gccatcgggg atgagctgcg ccggctgcgt ctggcggtca gggaacaggg ggcgcgggat
     4861 gatagttaag tttcatgcca ggggaaaagg tggtggcagt ggtccggttg attacctgct
     4921 gggaagggag cgtaaccggg aaggggcaac ggtgcttcag gggaatccgg aagaggtccg
     4981 ggaactcatc gatgccacgc catttgcgaa gaaatacacg tcaggtgttc tgtcgttcgc
     5041 ggagaaggag ctgccgcctg gagggcgtga aaaagtgatg gcgagctttg agcgtgtact
     5101 gatgcccggt ctcgagaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg
     5161 acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca
     5221 gccgtactac gaccgggcag acaggccgag aattgatgcc tggcagacgc tggtgaatca
     5281 ccattacggt ctgcatgacc ccaacgcccc ggagaaccgc cggacgctga cccttcctga
     5341 taacctgccg gaaacgaaac aggcgcttgc tgagggcgtc acacgcggga tagacgcgct
     5401 ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca cagaggcggg
     5461 gcttgaagtg gtcagggtga cgcgaagcag tatcagcatt gcagacccga acggcgggag
     5521 gaatatcaga ctgaaaggag cgttttatga gcaatctttt acagacgggc gcggagttcg
     5581 agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacgac g
//
