LOCUS       CP088368                5167 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F16EC0659 plasmid pF16EC0659-2, complete
            sequence.
ACCESSION   CP088368
VERSION     CP088368.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391701
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:27:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,993
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 83 of 220
            Pseudo Genes (incomplete)         :: 162 of 220
            Pseudo Genes (internal stop)      :: 37 of 220
            Pseudo Genes (multiple problems)  :: 52 of 220
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F16EC0659"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF16EC0659-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4714..5167,1..473))
                     /locus_tag="LQR08_24945"
     CDS             complement(join(4714..5167,1..473))
                     /locus_tag="LQR08_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGD47486.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            650..1048
                     /locus_tag="LQR08_24950"
     CDS             650..1048
                     /locus_tag="LQR08_24950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD47487.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(1252..2166)
                     /locus_tag="LQR08_24955"
     CDS             complement(1252..2166)
                     /locus_tag="LQR08_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGD47488.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            3903..4112
                     /locus_tag="LQR08_24960"
     CDS             3903..4112
                     /locus_tag="LQR08_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD47484.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(4178..4711)
                     /locus_tag="LQR08_24965"
     CDS             complement(4178..4711)
                     /locus_tag="LQR08_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD47485.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     Typing          complement(1392..2166)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(4..473)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.726"
                     /coverage="99.0"
                     /evalue="8.66e-100"
     Typing          complement(4718..5166)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.333"
                     /coverage="99.0"
                     /evalue="2.57e-90"
ORIGIN
        1 tcctttttcg cacctccccg ctcgggatac ctggcgttat agcgccggaa atactgttcc
       61 ggtgtacgtt caatgccatc attcacacgc tgactcatca tgatatgcgc atgaggctgt
      121 tcgccgccgt caatgctcgc tttcggattg tgaatggcaa aactccaggc gtgccgttcg
      181 ccagcttcct gacgtacaaa atcctgcacc agttcaaggc gctgttccgg tgtcagttca
      241 cggggtaatg caatttcaag ttccctatag gtcgaaccgt tcgcacgttc aaactggtca
      301 gccgcctgcc agaaatggga aggattatcc ctggcccatt ccggcatgtt tccgtgtgcg
      361 gaatattcca aatcctgacg gtcgcggtat ttctctttgc gctcgatgta atcggcgtgg
      421 tttgcagcct gccccttgcc accaaattta acgctgaggt gataggttgc cacggtttca
      481 gccctctgca ggagcgcact gtttctcgac gaaggagaga aacatataag tgcgcttcct
      541 tctaatttta cgaattagca cggaagatac cccctgcacc cgccgaccgc aagcgatata
      601 ctgcacgacc acaacacacc gcaaaaaaac accagggagc atcacgaaca tgcgcatcag
      661 caacatcgaa tggctgaaga aacggatcgg attcatcagg aagctcggag aacagaccgc
      721 acgacagaga cagattattg acctgatcga caacgaagcc ggactcacag aacaggaacg
      781 caaacttctc cacgtactgg caacagcgga aaaaaacgac ttacaggcac aggagagcga
      841 acgaaaacag gcggtccaaa aacgaatcga gggaaaaaaa caacgacggg aacgcaatca
      901 ccggctcttt ctcgcggctg ggctactgat cgaagcagga ctggtagaca caaaaacagg
      961 cgagctatgc tataaaaaag acaggatatt acaagcactt aaggaactta agtacgatct
     1021 ggagacatcc ccaaacccag acgcgtaagt gcctccatcc gtgttccgat tccctggtgc
     1081 ttcctcgctc attcgctcgc tacgctcggt catgagactg cggcgagcac taccaccaca
     1141 atcacgcgga aagccgcgcc attgcagggc tgaacgggga aaaacggcaa aaaacctgct
     1201 gctcaaaaag acgccagaaa ggcgctgtta tctgatatag gcttatttgg tctactctac
     1261 gagacctcgt tttttcagtt ggtaatacca ggcgcgactg atccctaatg cctcccacgg
     1321 tttcagcgtg cgtgcagatg tagctactgt gctgcgctta gacttagaac cgcccttgcg
     1381 accgcgtgca gcctgaattt ctggcgtgtg cgtatcagcc acatactgtg cgaaagtttc
     1441 cggcgtgaag ttcctgtgcg tgtatttcgc aatgctcttg ccaatagccc gacattcagc
     1501 cagtgaaagc ggaacgggaa gcgatgcatt gtacatttcg acacgctgga tcaccgcttc
     1561 aagccactgt gagaacgctg gccagccctg acgaatcgcc ctgtaagccc atttgcgcgt
     1621 catttcgaac aggtggcagt ttcgccccat cccgtaatgt ttatcgatgc tacgacgctc
     1681 tgaggcgctc aaatcgagat aatcagccag ttcatcgagg gtataggctt cctcgcgcca
     1741 ctccatcacc agccattcca ggtggaacgg atttttgcaa atcaggccgc tgtagttcac
     1801 atccgcacaa agtttctcac acagcgaacg ttcaaccgct gcggcatatt tcagcgcctt
     1861 aaccgaggca tcaggcgcgg ttctcacggc gatgttgagt gcgtagagca ggtgagcgtg
     1921 tccgttcaca ggatttttaa cggtgatgtt cggcgcggga gcgttccggt cgctccagtc
     1981 aatcgcagcc ccctcgcggt caacgtcaaa aaccagccag aactgcgcat gaggctggtt
     2041 ctgctgtatg taccgcgcaa gaagcgcacg ccctttgccg gagatgcgaa gaccaaaagc
     2101 gagatcatcc gtgtgatagg gacgatgggg taaattttcg tcgaagtatt gaagcgcggc
     2161 gctcatgtgt tacgattctc tccgggttat gcgacctaca cgccctctgt atttcatgtt
     2221 gaatcttgcc ggatctggac atgatttcag gggcgtttgt ttttcagtcg cttacactac
     2281 acccaacggc aaatcctgtc gaaccttccc gaaaacccga tccatttacc ataactcccg
     2341 ttgtacgccc cccttaaaac gccctcagtc gctttctggc gcaaaaaacg caaaaagaca
     2401 agaaaacacc aggccaaacc agaaaaacgc gtcagaatcg ttttcagggc gttgcataac
     2461 tatgcacgaa agcgatgtgc gatcggtttc gtgcgttctg aacaccgaac ggctcaaaac
     2521 gacaagtttt ctcccctggt gcgtggcgct ctgacggggt ttcctgtttc caaacttcac
     2581 atctcgccag gacaaaaaaa aaccgacctg gggtcggtgg caagaatcag agtctatggg
     2641 aaatatttta gttatcggat gctcaatatt cacaaacgca aaaaatgcct cagtgatgcc
     2701 ctaaaacaac ctttccagaa ggagtccgcg ctttacgatg cacggaagag ataaaaaacc
     2761 ccacaaaagt ggggaaccaa gagaaacaac aattacaggt tgttggttgt gttcgaacaa
     2821 cgatgaagta gctacgctaa ataataatta ccgtacaggc cctacatatt caacactaaa
     2881 tttaagatcc tgacgaagga tttataaatg cacgaaaaca catagacaga caaattcact
     2941 aaattcatac acaaaaacaa gtgtagtttt ctaagtaaat tagattcgac aaccaattag
     3001 tttatccaca tcgcattcca ctcttgctac accaatcaca tttcttgata tgtaatagca
     3061 gaaaaaataa aaccagacac caatagacca aaaaacaaac attcaacaag aaacattttg
     3121 aacaatatca caaaactgtc acaaccatca tcagactatt gaaaaaaact ggttgctgtt
     3181 ttataaaagc gactgtaaca acctccactc tatagttcca ttagtttaaa gtgtcacaca
     3241 aactgagcat ccatttgttt tccttacttt tgcccctgta ttccaagggg tttttttata
     3301 acttaaagat tagcacacca cacaaaaaca ataaaaaaaa acgacaagtt atacaataaa
     3361 accagtacta tccatacagc taattacata taaatactat taaaccatct ggacattaaa
     3421 aataaactct tataacataa aattaatgcg gttccggtac catatgtcag ggataatgtg
     3481 caaatggagg tatcggaact gcatttcagt tcattattaa ttaatgacaa aaaaaacctc
     3541 tgcgagcgca gagggggtaa ggaatgttcc cggtcataat atgtcgatag tgtttatata
     3601 catacagaac aagaacaaca accagccaac taataaatat tttatttttt agagctgaaa
     3661 ccttaaagct cacttccatt ccatacattg ttataaacct tatgttgaaa actgaaagta
     3721 agtacggcag acttattaag ttgcgtgcag tcttttcacg ttatacaaac aagaacacct
     3781 gcgaagttat ttctccttac atatccccct atgccaacgg gggatttttt ctggttcact
     3841 aacgcataaa taatagtgac accctatcat tcacaaaccg tgataatata aggattatca
     3901 tcatggcaaa attaggtgaa aacaccccac atattatcga tgctactgta gattttatgg
     3961 catcaagcca ggcatttcgt gagtatctga aaaaacttcc ccctgataac acaataccgc
     4021 cagctcttac tgatgagcac gtaccgttat atatacgaag gctggaatac tatcgtctgc
     4081 tttatcgacc aaagcagaca gaatcatatt gaaaaaaacg gtgaacggtt cgcgaaattc
     4141 tttcgcgggt cgtgaggcgt tggacaagcg aagcgcgtca gtttttccgg ctcttccccg
     4201 tgacaggttt ctccaccagt ttatcttcca gtgtcacaac tttctgatac accgccgctg
     4261 tggcgttgta gttcctgtcg agtattgttt tcattcccca tccgagcaca cagaacataa
     4321 ccagaaaagc cactgacacc ccacccagcc atttccagct atacccttta agggtgttgt
     4381 attcctgcac gatattgctg tgaattgtcg caatatccct gttcagggca tccgcgtttg
     4441 ccttcatgtt tgcgactgac tcttccagcc gctgaaaatc ctggctgatg cgctcaccta
     4501 tcagtcctgc cacgtccagc ttctgcacct catttctgac ttcatccctg acctgttttg
     4561 ccacaccgtc agaaatatcc cgatatccgc gaagagtttc ccttgccaca gccagagaat
     4621 cagcaacagc tttctgttgc tccattgcgg tatgttgcag atgctccacc gataacagga
     4681 gcgtaagtaa tttttcttct gtgctggcca tgttcagaat cccatagaaa atccacgtga
     4741 cggtgattta acccgttcag gtgcagttcg gttctgtctg ctcactctgt ctctcatgtc
     4801 aaagccatgc tcccgcgcaa catcatgttg cagccttctg accagttcat gttgcttaac
     4861 cggagtgtcc agccattccc tgatgtcatg cccgaccgtt cgcgcctgtt caagatggcg
     4921 ggaatagtcc ggttcagacc gtttttgcat gatgtcatga cgctgggcat catcaagccg
     4981 tccggcagct tcaaaaccca gatgcacttc gggacgtcgt tcaatgccct gttctttcag
     5041 cgtccggcag tcaatgaagc ccctttcaaa gccatgcttt ctcatatgtt cgttgtgctt
     5101 cacctcccag cgttttcgca attcccgcaa ctgctctttt tgctgagtga gagtcaggct
     5161 tccggaa
//
