LOCUS       CP088362                4234 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F17EC0098 plasmid pF17EC0098-6, complete
            sequence.
ACCESSION   CP088362
VERSION     CP088362.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391703
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,511
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 130 of 298
            Pseudo Genes (incomplete)         :: 213 of 298
            Pseudo Genes (internal stop)      :: 55 of 298
            Pseudo Genes (multiple problems)  :: 85 of 298
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F17EC0098"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF17EC0098-6"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(4080..4234,1..2065))
                     /locus_tag="LQQ82_27545"
     CDS             complement(join(4080..4234,1..2065))
                     /locus_tag="LQQ82_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGD37950.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPVRRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
                     /name="MobA/MobL family protein"
     gene            2244..2519
                     /locus_tag="LQQ82_27550"
     CDS             2244..2519
                     /locus_tag="LQQ82_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UGD37948.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNG"
                     /name="mobilization protein"
     gene            complement(2725..3633)
                     /locus_tag="LQQ82_27555"
     CDS             complement(2725..3633)
                     /locus_tag="LQQ82_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGD37949.1"
                     /translation="MSAALQYFEENLPHRPYHTDDPAFGLRISGKGRALLARYIQQNQP
                     HAQHWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNLAVRTAPDASVKV
                     LKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASE
                     RRSVDKHYGLGRNCHLFEITRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGLAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLDVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     Typing          1708..2066
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2067..2237
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="97.0"
                     /evalue="1.23e-74"
     Typing          complement(2906..3633)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.376"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          complement(9..2065)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.586"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cctgacagcc cttaagcctg aactgcatca gcttcggttc cggtgggtac ggagtatcag
       61 catcacggtt tatggcctca ccgtagcctg ccaggtgtat cccgttctct gtgatgatgc
      121 ttaccatcag atgactgtaa tccggcgacg gaagcgcaag aacgggacgc agctcgtcct
      181 ccaggcgcac aaaaccgaca agcatttcct ccggcccttt gcacaacagc agattcagcg
      241 tcctgctgtt tcgctctgtc gaaaagacaa cgggcatgag ttgctccggt gccgcataag
      301 cggtgacgtc cacccggtgc gctgacggca gcggagaggc tgcgggcctg cggtcgtcgc
      361 tgaaataaaa attatccggc cagtgatgcg gcagcgccgg aaaaccgtaa gagggccacc
      421 cctggcgcat ctgttccagt acatgccgga ccgtcgcacc ggaatacatt ctcacacgct
      481 caggccgcgc catcgtctca tgagattctg gcattccctc acgtatatcc gagaccatca
      541 cagccccgcc tggcgacagg taaacattca ccgggtcatg aacactctgg gggtaatgcg
      601 ccagctcatc accccatacc gtgaattctc cggctctgtt actcagcatc atgtagcggg
      661 ttatcctgcc cgttcccggc tctctggcac gccccagctc cgtaataacc ccggctaatg
      721 ccgtttctcg gctcaggcga tggttatcat ggtgtttcag ccgtgcgtca tattcctgtt
      781 ccttccggcg cgcttccgct tcggcttcct ccctgcgccg ctccgcttcc cgtgctgaca
      841 gtttttcttt cagctctgcc attcccggcg aaatccacgt attccactgc gtttcaaagg
      901 cctgacgacg gacgggaatg tcccgttctt tcacggccct gtaggtgcgg agaagagatt
      961 caatctgttt ctcgagtttc cagaagcgga gttgtctttt gcggtattcc ggttcagccc
     1021 atgtcatcag gggaagctct gacaggatgg acgctaccgg acgggctttt ctgagtccgg
     1081 tcatctcttt ttcgagaata tcccgctctt tcaccagcgc attcccctca cgtttgatgt
     1141 cgtttgcctt cccgttcatc gccacgaact ccggtgtcat ctcccatgcg ttcccgcatt
     1201 cctgcggcag tgtggcaatg aattcccgca gggtatccgc tgaggcattc gcggcgggaa
     1261 tggtcagatt aagggcttcc gctgcacgac gtgcggcatc gctgagttcc cgtttccgca
     1321 cgttctcctg tacctggatg actgccagcc ttgtgcggga aacctgtatt tccaggttaa
     1381 ggctgttaat caggcggttc tgctcaccgc gttctgtttc catgccccgt ttttccatcg
     1441 cacaggcagc ctttccgagg tgaaggcccg gcgctttctc cagtccctga cgctcgtatg
     1501 aacgatggtc tatctcttcc tggtagccgg cgttcgcaag cgctctgttc gcatggtcag
     1561 cccatgatgc gcgccacgtc tcggcgagtg cccggtcgtt ccagtccctg acctttcctg
     1621 cgaatcccgt ttccccgaca gctctcgtgg tcagcatgat gtgtgcatgg gggttggtcc
     1681 ggtccatatg atggaacgcc acatcggcaa tcatgccccg actgacaaag ttttcccgga
     1741 caaaagcgag aacggcctcc cgtgcggcct cgcgggaaat ctcacgggga atggcgagtt
     1801 caatctccct tgccagctgg gagttccggc gctgttcggc tttctcgacc gcattccaga
     1861 gaacggaacg gtcaccacac caggacggtg cattcgcagg ggtgagaatg accgcatcat
     1921 caacgccgcg tttacgggtg taatcgtgga ttaatccggt acggtcatcg ggtatgacag
     1981 agccggaacg gtaggcagca gaagcaacgg cactgtagcc gttttttcgc gaaatgattt
     2041 tcatgctgag atgataaatc gccatttctg ccgtaatgcc tttttgccgt tttctttcag
     2101 ccccgcaggg cgcacacaat cgaaacgaag tgaagattgt ataagtgcgc acttcgtgat
     2161 gtgccgtgca ttatgccagc tcagttttta aactaccagt gacgagctaa aatgcgattt
     2221 ttcaccgcgc aaggagtcac gaaatgagca gaacactcga acagaagatt gctgaagctg
     2281 aggccagatt gcagcgcctg aaggcgaaga gccggagcct ggacactgcg caaaaagtca
     2341 ttgtgggagc agccatgctg gcgagggtac ggaggccgga agaggcgcaa ctgcgtgcgt
     2401 tcctgctcca gttcctgcga aaggaagtca caagacaggc cgatgtgaac cggctacaac
     2461 cactgattaa cgagctggag aagctaccaa gaccacctgc gaaaccgcag aacggataag
     2521 ccccggcgcg taagtgccac atgcaccacc ggacgccttt gcgcttcctc gcacattcgc
     2581 tcgctacgct cggtcatgag gctgcggcga gcgctaccac cgcaatcacg aggaaaccag
     2641 cgccattgct gggcggaacc ggaaaaaccg caggaacgcc aacagctcaa aaaagcgccg
     2701 aaaaggcgct gttatctgat aaggctattt gtgcattgcc caattacgga tcgtatttcg
     2761 atgtacatcc agcatcgccg caatagcttt ctgagaatat ccattctcca gcatacatag
     2821 cgccatgaaa cgcttgtcct cgtaggcttc gcctttagca aggccacctt tccggcctcg
     2881 agcggcctgt acagcggaaa agccttccgg tgagaatttt ctgtgcgtgt atttcgcaat
     2941 gctcttgcca atagcccggc attcagccag tgaaagagga acgggaagcg atgcgttgta
     3001 catttcgaca cgctggatca cggcatcaag ccactgtgag aatgctggcc atccctgacg
     3061 aatcgctctg taagcccatt tacgcgttat ttcgaacagg tggcagtttc gccccagccc
     3121 gtaatgttta tcgacgctac gacgctctga ggcgctcaaa tcgagataat cagccagttc
     3181 atcgagggta taggcttcct cgcgccactc catcacctgc cattccaggt ggaacggatt
     3241 tttgcaaatc agtccgctgt agttcacatc tgcgtcaagt ttctcacaca gcgcacgttc
     3301 aattgcagcg gcgtatttca gcaccttaac cgacgcatca ggcgcggttc tcacggcaag
     3361 attgagcgcg tatagcaggt gagcgtgccc attcacggga ttttttacgg tgatgttcgg
     3421 cgcgggggcg ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa cgtcaaaaac
     3481 cagccagtgt tgcgcatgtg gctggttctg ctgaatgtac cgcgcaagaa gcgcacgccc
     3541 tttgccggag atgcgaaggc cgaaagcggg atcatccgtg tgatagggac ggtgaggtaa
     3601 attttcttcg aagtattgaa gcgcggcgct catgtgttac gattccttcc aggttatcga
     3661 ccgacatcgc ccctgtatct tcgtgaaagt cttggcggat ctacacgaat ttcaggggcg
     3721 tttgtttttc agtcgcttac actacaccca acggcaaatc ctgtcgatcc ttcccgaaaa
     3781 accgatccat ttaccataac tcctgttgta cgtacccctt aaaacgctct cagacgcgtt
     3841 ctgacgcaaa aaacacaaaa agacaagaaa acaccaggcc aaaccagaaa aacgcgtcag
     3901 aatcgttttc aggggtctgc ataactattc acgaaagcga tgcgcgatcg gtttcgaccg
     3961 ttcagaagcg cgaacgggtc aaaacggcaa actttctcat ccagaaaacg cctcacggga
     4021 cgcggaattt tttcgtgtcc cgtgagacgt tggcggggtt cggggtgcag ccccggatat
     4081 cacatactca tgccccggta atgtccgggg cgtcgttgtt gttgcctgtg ctgttcgtcc
     4141 cgcttccatt catgccaggt ctgattaaat ccgagttgac ggaagagata atccggcatc
     4201 tcttccggtt tgttgatggc agcaaaaagc gtgt
//
