LOCUS       CP088361                4593 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F17EC0098 plasmid pF17EC0098-5, complete
            sequence.
ACCESSION   CP088361
VERSION     CP088361.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391703
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,511
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 130 of 298
            Pseudo Genes (incomplete)         :: 213 of 298
            Pseudo Genes (internal stop)      :: 55 of 298
            Pseudo Genes (multiple problems)  :: 85 of 298
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F17EC0098"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF17EC0098-5"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            461..694
                     /locus_tag="LQQ82_27520"
     CDS             461..694
                     /locus_tag="LQQ82_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UGD37947.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1382..2221)
                     /locus_tag="LQQ82_27525"
     CDS             complement(1382..2221)
                     /locus_tag="LQQ82_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD37946.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(3232..3423)
                     /locus_tag="LQQ82_27530"
     CDS             complement(3232..3423)
                     /locus_tag="LQQ82_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UGD37944.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3486..3809
                     /locus_tag="LQQ82_27535"
     CDS             3486..3809
                     /locus_tag="LQQ82_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UGD37945.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4018..4452
                     /locus_tag="LQQ82_27540"
                     /pseudo=""
     CDS             4018..4452
                     /locus_tag="LQQ82_27540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3060..3144
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(2491..3049)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..792
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.66e-150"
     Typing          3800..4593
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.208"
                     /coverage="100.0"
                     /evalue="6.26e-163"
ORIGIN
        1 tgtagacaag gcactgagct taaacaggca gaaaatcaga gacgctatca gcgaacatac
       61 aacatccgtg aaacagcaac tggatacgct gagcaccacc gtgagcacgc agctcagcac
      121 cacagaggcc gaactgtccc ggcagcagaa aaacctgctc tggcaggtga tcaaagggcg
      181 ggtactgttt ccggcgctga ccgcgctcag cgtcacgggc ggcattttcc tggggtgctg
      241 ggggctgatt cagtggcagg aaagcaggat tgcgaaaaat atcttgacca tcagagagca
      301 ggaaaacacg ctggcgaaac tcgaggcaaa aacctggggg gtgacgttcg tcaatggcga
      361 gaacgggaaa tttctggtgc tgccggacgg ggtgaaaggg gaaaacacct ggacggtggg
      421 cgacaagaac gccgtgaggc tggtcaggga gtaaacgagt atgacggaac tggaagggca
      481 tttgctgaac gccttagagc atttgcaaca ggactatatg cggcggctga acgagtggga
      541 gagcgccttc gcagaattgc agaagatgca cgcggttacg caacagaaca acgcgatact
      601 gaacgagcgt gtcgtgaact tgagtcagca ggtgcagcta ttagccggac aagtcgaccg
      661 cttgagtcgg ttatttatca cgaacaacag ataagggatg aacaggagcg tcaggcgcag
      721 gagcttgccc ggcgctggca tgaagagcag gaacccgaag cgcggatctg gcgtggccca
      781 tcaatgggga tgtgaccggg gtaccccctg aaatcgagcc agtttgtggc acgccctacc
      841 cggatggcgc caatgttaat gtacgtacat tagcattaac aatatccaaa cctccccatt
      901 ttgccccgtt cgacggctgg aacgtgcgaa actgatcgtc attcagtttc gcgcataact
      961 atgcatgagg ttaaaattta ccaggcgcga tcgcggcagt ttttcgggtg gtttgttgct
     1021 gtttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt taagcggtac gcgcaatgcg
     1081 acgttatggt aaatacggag tattttaatt gcgggccttt gttgtatatc tctgtcgttt
     1141 gtccccgggg ggaagcatta ctgctaacaa gtcccgtgag aaaactgcgc aatcgtattg
     1201 cgcaccatga acctattctc aaccgcaatc tggaggatga ttttgctacg ataaagagaa
     1261 ttatcgcgta tcggtgtcag cattcattgg agtggatgct gaaaaaccag gtgttactcc
     1321 ctctacttac acttaaacct ctctaaaaat ataagtaagt actatcgagg gctatcatga
     1381 actatggtgc cctctgagca tagctaccca ttacttcttc tatgtcattg aataattcat
     1441 ttcttaactc taagtattct agtaactcca tttctaatga ctctgtcgtg aacttaaatc
     1501 tagaaggatc tttgattgag tttactagtc ttgataacgc tctatatgtt ttgagtgaag
     1561 gttgcattat agatggaaag tatttgaaaa cctcttcatc tctgttgaaa ttaactaaaa
     1621 acttgtagtc atcatttata aatctcaagc atacagcgtc aagcttatcg ccttttctta
     1681 ttattatttc tttataggaa agatattttt cagaagataa gtcccacatc aagcgaaaat
     1741 ctctctttgc gaacgagttc gcgtcatcat tgaaaagaga tgatgtgatg gctttggata
     1801 gaccttctgt gtttatctct ttgttggatg ggtagtaaat tttattaatt gctttcattc
     1861 tctcaaatga tgaatatagg tcattatgcc accttgttga aatcactcct ttagttatat
     1921 tctccttgct aaaattaagc cctgtatcaa aaataaatgc agccatcagt gaatatgata
     1981 tgttttttat taactcagac ttaaatggtt ccgctatgga gtagtacagt gataaaataa
     2041 ctaaaaataa aaggtattgt cttacctttc ccgatgagta tatgtaaagg cgatatttat
     2101 ttattgatag ttttattttg tatggaatga tctcatttct gtcatatcct cttcgtggtg
     2161 gagcaaaaca aaagaaacat gcagctatta ataaaacaaa aaagatgaac agtgtaagca
     2221 agttcggctc ctgtgtatgc tcactatgca tgattgtctt gagatctaac gatcctgaag
     2281 gtagtcttgt ccgtaaacga aaaaaccgcc cagcaaggcg gcttttcgaa ggttctcaga
     2341 gcagcaactc tttgaaccaa ggtaactggc ttggaggagc acagccacca aatctgtcct
     2401 ttcagtttag ccttaaccgg cgcataactt caagactaac tcctctaaac ggttaccaat
     2461 ggctgctgcc agtggcgttt ttacgtgtct ttccgggttg gactcaagac gatagttacc
     2521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2581 aacgacctac accgggccga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     2641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2761 ctgacttgag catctgtttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2821 cagcggcgcg gccttcttcc tgttcctggc cttttgctca catgtttctt ttggcgttat
     2881 cccctgattc tgtggataac cgcatcaccg tctttgagtg agctgatacc gctcgccgca
     2941 gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggc
     3001 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact tttcttataa
     3061 tccgcactga tgccacatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     3121 cagggctgcg ccccgacacc cgctaaaacc cgctgacgcg ccctgacggg cttgtctgct
     3181 cccggcatcc gctcacaggc aagctgtgaa cgtctccggg agctgcatgt ttcagttgct
     3241 ttcaccgtca tcgccgaagc gtgcgaggca actacggtag agttcgtcgg cgtggtcgtg
     3301 aagtgattcg cagatgtctg cctgttcgtc cgcgtccagc tcgttgagtt tttccagcag
     3361 ggtcagcgtc tggcttctga taaagcgggc catgttaagg gctgtttttt cctgtttggt
     3421 catggtctta aggctcctta ataggggatt ttttaggggg aatgataccg atgaaacgcg
     3481 agaagatgct cacgatacgg gttagcgatg aggagcacgc ccggttactg gaacgctgcg
     3541 agggtaaacg actggcagaa tggatgcggc gggtctgtct gggggagccg gttgcgcgca
     3601 caggaaaact gccgacacta tcgccgccat tgctgcggca tctggctgcc atcgggaaca
     3661 atctgaacca gacagcccgg aaggtgaaca gcggccactg gtcttcgatt gaccgggttc
     3721 atgtggtggc cgctctgatg gccatcgagg gtgaattgcg ccagttgcgg caggctgtca
     3781 gggaacaggg gggacgggat gatagttaaa tttcatgcca gggggaaagg cggtggaagt
     3841 ggcccggtcg attacctgct ggggcgggag cgtaaccgcg aaggcgcaac ggtgctccgg
     3901 ggtaacccgg aagaaatccg ggaactcatt gatgccacgc cattttcgaa gaaatacacg
     3961 tcaggcgttc tgtcgttcgc ggagaaggag ttaccgccgg gtgaacgcga aagagtgatg
     4021 acgagctttg agcgtgttct tatgcccggt ctcgaaaaag accagtacag cattctctgg
     4081 gtggaacacc aggataaggg gcggctcgaa ctgaattttg tcattccgaa catggaacta
     4141 cagagcggta agcgcctcca gccgtactac gaccgtgcgg acaggccgag gataaatgcc
     4201 tggcagacgc tggttaatca tcattacggg ctgcgtgacc cgaacgcacc ggagaacaga
     4261 cggacgcttg tcacaccgaa taatctgcca aaagcgaaac aggaggccgc agaagcgatt
     4321 acacggggtc ttgaggcgct ttaccgtgcc ggagcaataa aaacgcgtca ggatgtcact
     4381 gaggcgctca cagcggcagg ttttgaggtg gtgaggacaa ccagaagcag tatcagtatt
     4441 gccgacccgg agggggggcg gaatctcagg ctaaaggggg cgctgtatga gcaatctttt
     4501 acaaatggga gcggacttcg agaagaaact gaaagagcga gccgcttcta ccgagaacat
     4561 gctgaacagc gagttcagga agctagaaga gtc
//
