LOCUS       CP088360                5171 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F17EC0098 plasmid pF17EC0098-4, complete
            sequence.
ACCESSION   CP088360
VERSION     CP088360.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391703
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,511
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,095
            CDSs (with protein)               :: 5,095
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 298
            CDSs (without protein)            :: 298
            Pseudo Genes (ambiguous residues) :: 0 of 298
            Pseudo Genes (frameshifted)       :: 130 of 298
            Pseudo Genes (incomplete)         :: 213 of 298
            Pseudo Genes (internal stop)      :: 55 of 298
            Pseudo Genes (multiple problems)  :: 85 of 298
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F17EC0098"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF17EC0098-4"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            complement(join(5159..5171,1..914))
                     /locus_tag="LQQ82_27495"
     CDS             complement(join(5159..5171,1..914))
                     /locus_tag="LQQ82_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGD37943.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNSPAPNVTVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLDADANYSGLICKNPFHLEWQVKEWREEPYSLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            2652..2861
                     /locus_tag="LQQ82_27500"
     CDS             2652..2861
                     /locus_tag="LQQ82_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD37939.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(2927..3460)
                     /locus_tag="LQQ82_27505"
     CDS             complement(2927..3460)
                     /locus_tag="LQQ82_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD37940.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3463..4389)
                     /locus_tag="LQQ82_27510"
     CDS             complement(3463..4389)
                     /locus_tag="LQQ82_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGD37941.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4566..4964
                     /locus_tag="LQQ82_27515"
     CDS             4566..4964
                     /locus_tag="LQQ82_27515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UGD37942.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEDGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELYYKKDKILQVLKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(2..914)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.678"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3467..4389)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.078"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctcgttttt tcagttggta ataccaggcg cggctaattc caagagtctc ccacggtttc
       61 aacgttcgcg cagatgtggc aacagcacca cgcttagact tagcacctcc aattttcccg
      121 cccttgcgac cacgtgcagc ctgaatttct ggcgtgtgcg tatcagccac atactgtgcg
      181 aaagtttccg gcgtgaagtt cctgtgcgtg tatttcgcaa tactcctgcc aatagcccga
      241 cattcaggcg gtgaaagcgg aacgggaagc gatgcgttgt acatttcaac acgctgaatc
      301 acggcatcaa gccactgtga gaatgctggc cagccctgcc gaatcgccct gtaagcccat
      361 ttgcgcgtca tttcgaacag atggcagttt ctccccattc cgtaatgttt atcgatgcta
      421 cggcgcgctg aggcgctcaa atcgagataa tcagccagtt catcgaggga gtagggttcc
      481 tcgcgccact ccttcacctg ccattccagg tggaacggat ttttgcaaat cagcccgctg
      541 tagttcgcgt ctgcgtcaag tttctcacac aacgagcgtt caattgcggc agcgtatttc
      601 agtgccttaa ccgaactgtc cggtgcggtt ctcacggcga tattgagtgc gtaaagcagg
      661 tgggcgtgcc cgttcacagg atttttaacg gtgacgttcg gtgcgggtga gttccggtcg
      721 ctccagtcga tcgctgcccc cacgcggtca acgtcaaaaa ccagccagaa ctgcgcatga
      781 ggctggttct gctggatgta ccgagcaaga agcgcacgcc ctttgccgga gatgcgaaga
      841 ccaaaagcga gatcatccgt gtgataggga cggtggggta aattttcgtc gaagtattga
      901 agcgcggcgc tcatgtgtta cgattctctc cgggttatgc gacctacacg ccctctgtat
      961 ttcatgttga atcttgccgg atctggacat gatttcaggg gcgtttgttt ttcagtcgct
     1021 tacactacac ccaacggcaa atcctgtcga accttcccga aaacccgatc catttaccat
     1081 aactcccgtt gtacgccccc cttaaaacgc cctcagtcgc tttctggcgc aaaaaacgca
     1141 aaaagacaag aaaacaccag gccaaaccag aaaaacgcgt cagaatcgtt ttcagggcgt
     1201 tgcataacta tgcacgaaag cgatgtgcga tcggtttcgt gcgttctgaa caccgaacgg
     1261 ctcaaaacga caagttttct cccctggtgc gtggcgctct gacggggttt cctgtttcca
     1321 aacttcacat ctcgccagga caaaaaaaaa ccgacctggg gtcggtggca agaatcagag
     1381 tctatgggaa atattttagt tatcggatgc tcaatattca caaacgcaaa aaatgcctca
     1441 gtgatgccct aaaacaacct ttccagaagg agtccgcgct ttacgatgca cggaagagat
     1501 aaaaaacccc acaaaagtgg ggaaccaaga gaaacaacaa ttacaggttg ttggttgtgt
     1561 tcgaacaacg atgaagtagc tacgctaaat aataattacc gtacaggccc tacatattca
     1621 acactaaatt taagatcctg acgaaggatt tataaatgca cgaaaacaca tagacagaca
     1681 aattcactaa attcatacac aaaaacaagt gtagttttct aagtaaatta gattcgacaa
     1741 ccaattagtt tatccacatc gcattccact cttgctacac caatcacatt tcttgatatg
     1801 taatagcaga aaaaaataaa accagacacc aatagaccaa aaaacaaaca ttcaacaaga
     1861 aacattttga acaatatcac aaaactgtca caaccatcat cagactattg aaaaaaactg
     1921 gttgctgttt tataaaagcg actgtaacaa cctccactct atagttccat tagtttaaag
     1981 tgtcacacaa actgagcatc catttgtttt ccttactttt gcccctgtat tccaaggggt
     2041 ttttttataa cttaaagatt agcacaccac acaaaaacaa taaaaaaaac gacaagttat
     2101 acaataaaac cagtactatc catacagcta attacatata aatactatta aaccatctgg
     2161 acattaaaaa taaactctta taacataaaa ttaatgcggt tccggtacca tatgtcaggg
     2221 ataatgtgca aatggaggta tcggaactgc atttcagttc attattaatt aatgacaaaa
     2281 aaaaacctct gcgagcgcag agggggtaag gaatgttccc ggtcataata tgtcgatagt
     2341 gtttatatac atacagaaca agaacaacaa ccagccaact aataaatatt ttatttttta
     2401 gagctgaaac cttaaagctc acttccattc catacattgt tataaacctt atgttgaaaa
     2461 ctgaaagtaa gtacggcaga cttattaagt tgcgtgcagt cttttcacgt tatacaaaca
     2521 agaacacctg cgaagttatt tctccttaca tatcccccta tgccaacggg ggattttttc
     2581 tggttcacta acgcataaat aatagtgaca ccctatcatt cacaaaccgt gataatataa
     2641 ggattatcat catggcaaaa ttaggtgaaa acaccccaca tattatcgat gctactgtag
     2701 attttatggc atcaagccag gcatttcgtg agtatctgaa aaaacttccc cctgataaca
     2761 caataccgcc agctcttact gatgagcacg taccgttata tatacgaagg ctggaatact
     2821 atcgtctgct ttatcgacca aagcagacag aatcatattg aaaaaaacgg tgaacggttc
     2881 gcgaaatcct ttcgcgggtc gtgaggcgtt ggacaagcga agcgcgtcag tttttccggc
     2941 tcttccccgt gacaggtttc tccaccagtt tatcttccag tgtcacaact ttctgataca
     3001 ccgccgctgt ggcgttgtag ttcctgtcga gtattgtttt cattccccat ccgagcacac
     3061 agaacataac cagaaaagcc actgacaccc cacccagcca tttccagcta taccctttaa
     3121 gggtgttgta ttcctgcacg atattgctgt gaattgtcgc aatatccctg ttcagggcat
     3181 ccgcgtttgc cttcatgttt gcgactgact cttccagccg ctgaaaatcc tggctgatgc
     3241 gctcacctat cagtcctgcc acgtccagct tctgcacctc gtttctgact tcatccctga
     3301 cctgttttgc cacaccgtca gaaatatccc gatatccgcg aagagtttcc cttgccacag
     3361 ccagagaatc agcaacagct ttctgttgct ctattgcggt atgttgcaga tgctccaccg
     3421 ataacaggag cgtgagtaat ttttcttctg tgctggccat gttcagaatc ccatagaaaa
     3481 tccacgtgac ggtgatttaa cccgttcagg ttcagttcgg ttctctctgc tcactccgtc
     3541 tctcatgtca aagccatgct cccgcgcaac atcatgttgc agccttctga ccagttcatg
     3601 ttgcttaacc ggtgtatcca gccattccct gatgtcatgc ccgaccattc gcgcctgttc
     3661 aagatggcgg taatagtccg gttcagaccg tttttgcatg atgtcatgac gctgggcatc
     3721 atcaagtcgt ccggcagctt caaaacccag atgcacttcg ggacgtcgtt caatgccctg
     3781 ttctttcagc gtccggcagt caatgaagcc cctttcaaag ccatgctttc tcatatgttc
     3841 gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt gctgagtggg
     3901 agtcaggctt ccggaatcct ttttcgcacc tccccgctcg ggatacctgg cgttatagcg
     3961 ccggaaatac tgttccggtg tacgttcaat tccatcattc acacgctgac tcatcatgat
     4021 atgcgcatga ggctgttcgc cgccgtcaat gctcgctttc ggattgtgaa tggcaaaact
     4081 ccaggcgtgc cgttcgccgg cttcctgacg tacaaaatcc tgcaccagtt caaggcgctg
     4141 ttccgctgtc agttcacggg gtaatgcaat ttcaagttcc ctataggtcg aaccgttcgc
     4201 acgttcaaac tggtcagccg cctgccagaa atgggaagga ttatccctgg cccattccgg
     4261 catgtttccg tgtgcggaat attccaaatc ctgacggtcg cggtatttct ctttgcgctc
     4321 gatgtaatcg gcgtggtttg cagcctgccc cttgccacca aatttaacgc tgaggtgata
     4381 ggttgccacg gtttcagccc tctgcaggag cgcactgttt ctcgacgaag gagagaaaca
     4441 tataagtgcg cttccttcta attattcgaa ttagtacgga agataccccc tgcacccgcc
     4501 gaccgcaagc gatatactgc acgaccacaa accgccgtaa aaaaacacca gggagtacca
     4561 caaacatgcg catcagcaac atcgaatggc tgaagaaacg gatcggattc atcaggaagc
     4621 tcggagaaca gaccgcgcga cagagacaga ttattgacct gctcgacaac gaagacggac
     4681 tcacagaaca ggaacgcaaa cttctccacg tgctggcaac agcggaaaaa aacgacttac
     4741 aggcacagga gaacgagcga aaacagacga tccagaaacg aatcgaggga aaaaaacaac
     4801 gacgggaacg caatcaccga ctctttctcg cggctgggct actgatcgaa gcaggactgg
     4861 tagacacaaa aacaggcgag ctatactata aaaaagacaa gatattacaa gtacttaagg
     4921 aacttaagta cgatctggag acatccccaa acccagacgc gtaagtattc ccgttcacat
     4981 tctggttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     5041 gagcactacc agcgcaatca tgcggaaaga aacgccattg ctgggataaa cgacgaaaaa
     5101 cctacgactc aaaaggacgc aaaaaaggcg ctgttatctg atataggttt tatttggtct
     5161 actctacaag a
//
