LOCUS       CP088350                4072 bp    DNA     circular BCT 09-DEC-2021
DEFINITION  Escherichia coli strain F17EC0190 plasmid pF17EC0190-2, complete
            sequence.
ACCESSION   CP088350
VERSION     CP088350.1
DBLINK      BioProject: PRJNA782071
            BioSample: SAMN23391706
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Chromosomal integration and amplification of group 9 blaCTX-M genes
            in Escherichia coli expand the spectrum of resistance under
            antimicrobial pressure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Yoon,E.-J., Choi,Y.J., Kim,D., Won,D., Choi,J.R. and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, Gangnam Severance Hospital,
            Yonsei, Seoul 06273, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: other: SMRTLink v. v9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/02/2021 11:37:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,172
            CDSs (total)                      :: 5,056
            Genes (coding)                    :: 4,855
            CDSs (with protein)               :: 4,855
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 91 of 201
            Pseudo Genes (incomplete)         :: 134 of 201
            Pseudo Genes (internal stop)      :: 40 of 201
            Pseudo Genes (multiple problems)  :: 52 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F17EC0190"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pF17EC0190-2"
                     /geo_loc_name="South Korea"
                     /lat_lon="35.18 N 129.08 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            join(4066..4072,1..2153)
                     /locus_tag="LQQ64_25850"
     CDS             join(4066..4072,1..2153)
                     /locus_tag="LQQ64_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UGD23102.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(2409..3101)
                     /locus_tag="LQQ64_25855"
     CDS             complement(2409..3101)
                     /locus_tag="LQQ64_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UGD23100.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3633..3887)
                     /locus_tag="LQQ64_25860"
     CDS             complement(3633..3887)
                     /locus_tag="LQQ64_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UGD23101.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          2..352
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.011"
                     /coverage="98.0"
                     /evalue="1.1e-172"
     Typing          complement(3889..4065)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(2410..3101)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..2150
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.581"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttatcatct cagcatgaaa attatctcca gaagcagcgg ctacagcgcc gtagcttccg
       61 ctgcctatcg ttccggttca ctgatgcttg atgagcgtac cggactgact cacgactaca
      121 cccgcaaaag cggtgtggct gaagctgtga ttctgacccc cgcgacagcc cctgcctggt
      181 gtacgaaccg tgccgaactc tggaatgccg ttgagaaggc cgaatgccgg aagaactcac
      241 aacttgcgcg ggaaattgaa ctcgctatcc cccgtgaact gccgcaggat gccgcacggg
      301 aaaccgttct cgcctttgtc agagaaaact ttgtcagtca gggcatgatt gctgatgtgg
      361 cgttccatca catggataaa acaaatcccc atgcgcacat catgctgacc acgagggctg
      421 tcggacctgc tgggttcggc ggaaaggtca gggactggaa cgaccggaca catgccgaga
      481 cgtggcgtgc atcatgggct gaccatgcaa acagagcact ggcgaacgcc ggttatcagg
      541 aagagataga ccatcgttca tacgagcgac aggggcttga gaaaacgcct ggtattcacc
      601 tcggaaagtc tgcctgtgcg atggaaacac gcggtatcga aacagaacgc ggcgaacaga
      661 atcgtctgat taacaggctt aacctggaaa tacagatttc ccgcactgaa ctcaggaaca
      721 gcgggctgac agagcctgcc cgccgtgtgg ctgaccttct gaatatcgtg cttcctgaca
      781 acgcaacttc gtacacacta cgtgacatca tcgaagcgtt gccgaaggac agtaatgcgg
      841 catggaaact caccagtaat gccatgtcga tgatggcaga tatgcaggcg acgcgcagac
      901 gctgggaaga gctgaacacc gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa
      961 aatcatcgcc gttttcgtcc ggtttttccc gtattcccct gatgcaatgg gcggcaccgg
     1021 aataccgtag cgaacaggga aaaatccagt cgctgagcca gcaaatggaa aaattgcgac
     1081 agcattaccg gaaggtggag aaacaggaca ttcccgcgag tcagacggca tttgaaaaac
     1141 agtggcagca gtggggggca tccggccttg cagctctcag agtccagttg cttaaacgtg
     1201 aagaggaatt gcgccggaag gagcaggagg atacagaacg tagtcgcgcg gagcgacgag
     1261 cggaacagct caggaaaaat gacaatgccg ttatggattc aggggtactg agcgcagtcg
     1321 ttacggggta tggcgaggcc cccatgcctg gtaacgggga aatgacctgt taccttctgc
     1381 ttcacaaccg gaacggagaa tacacgctct gggggaacga gctggagaaa taccgcacca
     1441 gaatctttga gcgtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg
     1501 aaatcggtca acatccacca ctgcaacgtg tttattcctc tgcgaccttt gagcaacttc
     1561 tgacgcaggt ttgccagaca tggccgcaat atacccgcaa tctccgccag ccgaagacat
     1621 ggccggagtc tttctgtctt ggagaagacc gccagccagc catgccatca ctggcagcaa
     1681 ggaaggtgga ttttacgcag ggacgtctgc taccgacgct gatgccggta atgagttctg
     1741 tcgaccggga aacccgccag cttcagcttt tgctggtcat gggcgtggat gactctctgg
     1801 gcggtgttgt ccggcttaat ggtacgctgt atcccgcgtt tgccgttccc tcagccgata
     1861 acagccagct ggtcatcagt gcactgaccg ataaggggtt acggtatgca gggtatggtg
     1921 ttgccgtaaa tcacgatgca gacagccaca tatcacctgc gccggaactt atggagtttc
     1981 atctgaaaac ccgtgaggca ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg
     2041 atcatctgtt ccgctctctg ggtttcaatc gtacctggga tgaatggcgg cgcgaggaag
     2101 atgcaagaac acacaccacc gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa
     2161 caatattttg ttttgtttaa catcgtgtta tgttcgtcga tgttcttcta tgttcatcga
     2221 cagggatgaa tgcaagaagc acacacataa tgcaagcata gttttgtttt tccgccccca
     2281 aaaattgggg gttctttttt cgctgcatta gagcgctttt gacgcaattt ttcacttcaa
     2341 tagtaccttc gcactactaa cacaaaaaaa acgcagcagg gagcgtttta ggtgccttaa
     2401 aatcgatttt agatcgtcat cttgatctgt ttgtcttcct tgaagcgaaa atgcagggct
     2461 acaaccgtgc gccccttttt gatcgtctca accgttacag ccaggtcgct ttttttgttc
     2521 agttcgtcaa cacagggttt tattagtatt ttatttaagt ctctgaattg tttgtattta
     2581 ttttcaatcc ctaacattga acgaaagtca ttaagagcaa taattctctc tcccgtactg
     2641 cggaattgct ggaggaactc gtatattctt atactgtaac tacttgataa acctgatatg
     2701 ttttttataa caacgcgcgt gaattgcccc ttcagttgcg tcagataagg cattaccgcg
     2761 tcagaaaagg ttattcgagc cttggcctca cctttagcgt actgtgcccg atactggatc
     2821 cagcggaact cctcacgctt ctcgtcattc ttgagaatga tcgacctgtc ccataaacga
     2881 tcgatcgctt cctgtaactg aacctcggca tttttccgcc ccatgtcagg gaatgaatca
     2941 aaatattccg cagcggtaat ggtcattgtc ttttgcaatt cagcatcagc accagatttc
     3001 aatcgactga tacacagcaa cagaaaacgt tgttcctgta gggtcaactt gtagctggcc
     3061 tcaaccaaag cattcgactt gtaggcaatc aagtccgaca tacaaagatc ccgttcaaat
     3121 gtggtgatgc tttaatgtgc cccacaaata agaaatgtgg aatatttttt tttatatcct
     3181 cacatttctg tgaataacac cttaaattcc tccaccttta ccttaaattc ctccacctaa
     3241 acaccttaaa ttcctccacc tttaccttaa attcctccac atatacctct ataacatatt
     3301 gattttatta atgaattttg accctaaaac aagtattaaa aacaagatct taaaaacaag
     3361 atctaaaaac ttaacggcga gcctgtggat aacttggggc gctgaaaaca aaaaaccaat
     3421 aaaaacaatg tgttgcaaga aagcagaatt gcaagttccg aatactcccc ttccaaccct
     3481 gtggaaatcc agtattcatg cgggttcagc gcatatcacc taccgaaaaa ccgtgcgtat
     3541 gtgctcaacc tggcgacgga ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg
     3601 gtcgctgggg ctgcggcgag cggacgatac aatcactgtc ctggcagcgc ctccagctcg
     3661 ttaatcagcg gcagtatgcg cgtcacatca gcctgtcgtg tcacctctgc cttcaggaac
     3721 tggagtaacc aggcacgcaa ctgcacctcc tccggctttc tgacctttgc cagcaatgct
     3781 gcacccacaa caaccttctg cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt
     3841 aatctcgcct cagcatcagc aatcttctgt tcgagcgttc tgctcatgat gtaactccgt
     3901 gcgcgatgaa aaatcgcatt ttagcgcgtc actggtagtt taaaaactga gctggcataa
     3961 tgcgcgacaa atcacaaagt gcgcacttat acaatctcca ctccgtttcg attgtgtgcg
     4021 ccctgcgggg ctgaaagaaa actacaaaaa ggcattacgg cagaaatggc ga
//
