LOCUS       CP086677                4352 bp    DNA     circular BCT 22-NOV-2021
DEFINITION  Klebsiella pneumoniae strain SCPM-O-B-8923 (3TKP/19g) plasmid
            pB-8923_6, complete sequence.
ACCESSION   CP086677
VERSION     CP086677.1
DBLINK      BioProject: PRJNA269675
            BioSample: SAMN18928741
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow region 142279, Russia
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow region 142279, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 688.23x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/12/2021 15:05:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,091
            CDSs (total)                      :: 5,965
            Genes (coding)                    :: 5,801
            CDSs (with protein)               :: 5,801
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 66 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 26 of 164
            Pseudo Genes (multiple problems)  :: 25 of 164
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-8923 (3TKP/19g)"
                     /isolation_source="trachea"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB-8923_6"
                     /geo_loc_name="Russia: Moscow"
                     /collection_date="2019-11-21"
     gene            complement(87..302)
                     /locus_tag="KIF80_030430"
     CDS             complement(87..302)
                     /locus_tag="KIF80_030430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UFH95360.1"
                     /translation="MQKPKRKLGFMVGKSNATLPPDDETEAELDQEVLKLFYPNQCTKD
                     TIERSEAGTDVFTASGSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            603..1469
                     /locus_tag="KIF80_030435"
     CDS             603..1469
                     /locus_tag="KIF80_030435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UFH95361.1"
                     /translation="MFKIENNKDILKSQIPLKPYASNDLDFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMVFDIDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPYSPHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTGLAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYIN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQKARRSKRTEKQKLHTKTLLEK
                     AIMINEE"
                     /name="replication initiation protein"
     gene            1473..1745
                     /locus_tag="KIF80_030440"
     CDS             1473..1745
                     /locus_tag="KIF80_030440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="UFH95362.1"
                     /translation="MTALHTVKRNKTAKEMAQRLNISDRTVRKLIALPREDYEKEAKQR
                     RLKAFVLRESGMKWADVGIALGTTKHGAIALYKRYQQIDAPIKEA"
                     /name="HTH domain-containing protein"
     gene            complement(1754..3223)
                     /locus_tag="KIF80_030445"
     CDS             complement(1754..3223)
                     /locus_tag="KIF80_030445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UFH95363.1"
                     /translation="MKGNVAKTGKSFKNRVDYILKDDHSFICSNMASDYNNVSDLTDEF
                     KNVSSFRPDIKKPVFHAFLSLPKDEKLTDEQWQEIAKDYLKEMNIDIEKHQYICVRHKD
                     TDKDHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITDGLKGQKSEVSAPT
                     KNEIEQALRKGEKPARIVLQNALQAAMLGKPDLQTFIDRVQAVGIEPRFNVASTGNVAG
                     CSFSVGDVAFKGSQLGKKYSWNTIKNKVIYDKNRDDELVRRFSARKADDENSIGRSFVE
                     SNINDSRAVDGIDDDIIRISQHSIKSDTTDTYEGKQLVRSNNHSFKLSEKYNRPTNKKI
                     GRATHKAAQILNRVKKQYNKEPIKTYNKSSFNRGSNTFSGSSISRILDHAETATTATNN
                     FRLKGRRSFNSRATKIEEIRGRLGIEKPVIQPRKIVKEEIKKKPLGLSMNENRFKSKLK
                     EALKKEKPKNETTPLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3220..3588)
                     /gene="mobC"
                     /locus_tag="KIF80_030450"
     CDS             complement(3220..3588)
                     /gene="mobC"
                     /locus_tag="KIF80_030450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="UFH95358.1"
                     /translation="MDTDRKRGRIGLNHEVRRDISIQFRVNKAEFDYLKELVEASGLER
                     GHYIRKEILNSEPVIKRKMPEFEQSLYREIQHQGNNINQIAKKLNTNEDFKQSVAAEAN
                     FAIKQLMKRILKACEVYV"
                     /name="mobC"
     gene            complement(3601..3858)
                     /locus_tag="KIF80_030455"
     CDS             complement(3601..3858)
                     /locus_tag="KIF80_030455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UFH95359.1"
                     /translation="MKIVLSVSKCLYINDLDRLLPCDRFFVHLDYKDISKKESFEMNRH
                     DQESYKLIFDKIVNNDVMLAIDEDIYSEVIENLLYYSNEK"
                     /name="hypothetical protein"
ORIGIN
        1 gatatactgc atcgagggtt ggccttttca tcgtgtccgg ggtttggttt gttccaactg
       61 gtgaagatgg ccatctctcc cctgccctac agcttcagtt ttttgaacag gtcatcagaa
      121 ccggaggccg tgaaaacgtc tgtaccagct tctgagcgtt ctatggtgtc tttggtgcat
      181 tggttaggat aaaacagctt caggacttcc tgatcaagct ctgcttctgt ttcgtcatct
      241 ggtggcaatg ttgcattgct cttaccaacc atgaagccca gcttacgctt tggtttctgc
      301 atctcatgct ctcctatcca gcacagccag atcttacata gagtttagta tacgttacag
      361 tgtgacttac tctgtagatg gccttcggcc tgtgcttcgc tgttgtgtag ttttccaaaa
      421 actccgtcta atacctgata ttggtagact tcctacttaa caactttttt tgccggatct
      481 attgacaagt ttttttggta tagtaaaact gtaagagatt ataaataaaa gaaaagcccg
      541 cgttctctga ttagtttggc gaccagtgag agaacggggc attacagaaa ggatactgct
      601 atatgttcaa gatagaaaat aacaaagata tattaaaaag tcaaatacct ttaaagccgt
      661 atgcatcaaa tgatcttgat tttggtttga ctattcaaaa taagaataaa gcattagata
      721 tgctttattt acaagtaaat cacccgcttt atcttcatac aatggtattt gatatagaca
      781 aagaaaactg cttttacgag tttgaaaatg cccatttgcc tataccgtct tttataacaa
      841 aaagccctga tagtggacgt tgccattatg gttatatgct taatgctcct atatcttcta
      901 cagagaaaag cagacagaaa cctgtaaagt ttgccagagc attatattat aatatggcta
      961 ctcgtttagg tgctgatctt ggttatgctg gactgattac taaaaatcca tacagtccgc
     1021 attggtcgcc gttctggtca ggtgctgatt tatacgaact taatgattta gctgattgtt
     1081 ttgatgattt agaagaacca aaaaaacgag aaaatacggg gcttgctttt gggcgtaatg
     1141 ttgaaatgtt cgacaccata aggcaatggg cttataaaaa tgtgctaaaa tatcagagtg
     1201 aaagctcatt taatgatttt cacaatgaac tattgctaaa gtgtcaaatg cataatgcat
     1261 atataaatgc tgatgattta ctgccatata atgagataaa agccacagca aaaagtatag
     1321 caaagttctg ttggaaagaa ttcagcgaag aaaaactcaa caagatacaa agtaaaaaac
     1381 agaaagcaag aagatctaaa agaacagaaa aacaaaagct acatacaaaa acacttttag
     1441 aaaaagcaat aatgattaat gaggagtaaa atatgacggc attacataca gtaaaaagaa
     1501 ataaaacagc caaagaaatg gctcaaagat taaatatcag tgatagaacc gtcagaaagt
     1561 tgattgcttt acctcgtgaa gactatgaga aagaagcaaa acaaagacga ttaaaagcct
     1621 ttgtattacg tgaaagcggg atgaaatggg cagatgttgg tattgctctt ggaactacaa
     1681 aacacggtgc tatagctctt tataaacgtt atcaacaaat agacgcacca ataaaagagg
     1741 cttaaagcct cttttatctt ggtttaaaac ctcttggttt taaatgctga tcgtcttcct
     1801 gctgtagtgg tgttgtttca ttttttggtt tttctttttt aagagcttct tttaacttcg
     1861 atttaaatct attttcattc atactcaaac ctaaaggttt tttctttatt tcttctttaa
     1921 ctattttcct tggttgaata actggttttt ctatcccaag tctacctctt atttcttcga
     1981 tcttagttgc tctactattg aaactgcgac ggcctttgag tctaaagtta ttcgtggctg
     2041 ttgtggctgt ttctgcatga tcaagtatcc tactgataga actgccgcta aatgtattgc
     2101 tcccacgatt aaagctactt ttattataag ttttgattgg ttctttattg tattgttttt
     2161 taactctgtt gagtatttgt gcagctttat gtgttgctct tccaatcttt ttgtttgttg
     2221 gtctattata tttttcgctg agtttaaagc tgtggttgtt gcttctaacg agttgtttac
     2281 cctcgtaagt gtctgttgtg tctgatttaa tgctgtgctg actgattcgg ataatatcat
     2341 catcgattcc gtcaacggct ctactgtcat tgatatttga ttcaacaaat gatcgtccaa
     2401 tactattttc gtcatctgct tttcttgctg agaatcttct aacgagttca tcatctctat
     2461 ttttgtcata tatcaccttg ttttttatag tgttccaact gtatttttta cctaactgcg
     2521 aacctttgaa ggccacatct cccacactga aagaacaacc cgccacgttg ccagtgctgg
     2581 caacattgaa ccttggttca atgcctacgg cctgcactct gtcaataaac gtctgcagat
     2641 ctggtttacc caacatagcc gcctgtaatg cgttctgtag aacgatacga gctggtttct
     2701 cacctttgcg tagggcttgc tcgatctcgt tcttggttgg tgctgatacc tctgattttt
     2761 gaccttttaa gccatccgtg atggttaagc cgtgttttac ttctaaacgt tcacaagctg
     2821 ctattgtgtt aaatgctgag tgctgaccaa gccataccga accatccagc ccaaccctat
     2881 ttgctacaat atgaatatgg tctttatctg tatctttatg acgcacacaa atatattgat
     2941 gtttttctat atcgatattc atttccttca aatagtcttt tgctatttct tgccattgtt
     3001 catctgtgag tttctcatct tttggaagag ataaaaaagc atgaaaaaca ggttttttta
     3061 tatcaggtct aaagctgctt acgtttttaa actcatcagt taaatcacta acattattat
     3121 aatcacttgc catattgcta caaataaaac tgtgatcatc ctttaatata taatcaactc
     3181 tatttttaaa agacttgccc gtcttggcta catttccttt catacataaa cctcacaagc
     3241 ttttaatatt cttttcataa gttgctttat agcaaagtta gcttctgcgg ctacagattg
     3301 tttaaagtct tcattcgtat ttagcttttt agcaatctga tttatattat taccttgatg
     3361 ttgtatttct ctgtataatg attgttcaaa ctcaggcatt tttcttttta taacgggttc
     3421 agagtttaaa atctcttttc ggatgtaatg acctctttca agccctgatg cttccacaag
     3481 ctcttttaaa taatcaaact ctgctttgtt tactctaaac tgtatagata tatctctacg
     3541 tacttcgtga tttaatccta tacgccctct ttttctatct gtatccatat atataactcc
     3601 ttatttctca tttgaataat aaagtaagtt ttcgataact tcgctgtaga tgtcttcatc
     3661 aatagcaagc attacgtcat tatttacaat cttatcaaag ataagtttgt aactttcttg
     3721 gtcgtgtcta ttcatctcaa atgattcctt tttgctaata tccttataat ctaaatgaac
     3781 aaaaaaacga tcacacggaa gtaagcgatc taaatcgttt atgtataaac attttgatac
     3841 tgataaaact attttcatat ttattccttt ttgttttgaa gtgaactgta aaactattta
     3901 ccgcttcccc ttgatagggg aataagcgga agggtagatc ttttaacaac cctccgaacg
     3961 aagtgagagg gggcaacctg aaaggttggg tataataccg gctacgggat tacaccttgc
     4021 cttagtttcc gaaaaacaaa aataaagctg aactcaacct atatttaaat aaataaccga
     4081 tctaaaaagt ttgtcaatat agcggctaca taaataaata ccctgcacaa caaataaaat
     4141 gcaaataaaa aatagggtaa ataatatatt gtcttgatgg gtagctgtgc gtaataggta
     4201 gctttgcgta aaaaatgccg ctgctacgtg aaatctggaa aagttcgtga ccatcagatc
     4261 accagatttt aaggaatggg gtttgatcgg cggcgacaga aacgggcaaa acttgctttt
     4321 gtgcgtttgc tggcgtctat gggtttaaaa tg
//
