LOCUS       CP086676                9294 bp    DNA     circular BCT 22-NOV-2021
DEFINITION  Klebsiella pneumoniae strain SCPM-O-B-8923 (3TKP/19g) plasmid
            pB-8923_ColRNAI, complete sequence.
ACCESSION   CP086676
VERSION     CP086676.1
DBLINK      BioProject: PRJNA269675
            BioSample: SAMN18928741
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow region 142279, Russia
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Fursova,N., Kuzina,E., Novikova,T., Kislichkina,A., Sizova,A.,
            Mukhina,T., Solomentsev,V. and Bogun,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow region 142279, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 688.23x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/12/2021 15:05:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,091
            CDSs (total)                      :: 5,965
            Genes (coding)                    :: 5,801
            CDSs (with protein)               :: 5,801
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 66 of 164
            Pseudo Genes (incomplete)         :: 102 of 164
            Pseudo Genes (internal stop)      :: 26 of 164
            Pseudo Genes (multiple problems)  :: 25 of 164
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-8923 (3TKP/19g)"
                     /isolation_source="trachea"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB-8923_ColRNAI"
                     /geo_loc_name="Russia: Moscow"
                     /collection_date="2019-11-21"
     gene            142..1827
                     /locus_tag="KIF80_030375"
     CDS             142..1827
                     /locus_tag="KIF80_030375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UFH95354.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            1837..2094
                     /locus_tag="KIF80_030380"
     CDS             1837..2094
                     /locus_tag="KIF80_030380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="UFH95355.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            2179..2328
                     /locus_tag="KIF80_030385"
     CDS             2179..2328
                     /locus_tag="KIF80_030385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UFH95356.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3809..5773
                     /locus_tag="KIF80_030390"
     CDS             3809..5773
                     /locus_tag="KIF80_030390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UFH95357.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            5773..6504
                     /locus_tag="KIF80_030395"
     CDS             5773..6504
                     /locus_tag="KIF80_030395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="UFH95347.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            6511..7041
                     /locus_tag="KIF80_030400"
     CDS             6511..7041
                     /locus_tag="KIF80_030400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UFH95348.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7069..7248)
                     /locus_tag="KIF80_030405"
     CDS             complement(7069..7248)
                     /locus_tag="KIF80_030405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UFH95349.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7317..7694
                     /locus_tag="KIF80_030410"
     CDS             7317..7694
                     /locus_tag="KIF80_030410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UFH95350.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(7756..8055)
                     /locus_tag="KIF80_030415"
     CDS             complement(7756..8055)
                     /locus_tag="KIF80_030415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UFH95351.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8045..8308)
                     /locus_tag="KIF80_030420"
     CDS             complement(8045..8308)
                     /locus_tag="KIF80_030420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="UFH95352.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(8614..9033)
                     /locus_tag="KIF80_030425"
     CDS             complement(8614..9033)
                     /locus_tag="KIF80_030425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UFH95353.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     BGC             1..6827
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2432..3010)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5774..6501
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tcattttcac aaaaacgcaa atttacagac ttgacatgaa aaacaaggca gcgtaatttt
       61 tatactgtat ataaaaccag tggttatgtg tacagtattt tattttagtt tattgtttta
      121 aaagtcaaag aggaaaagta tatgagtggc ggagacggac gaggtccggg taattcaggt
      181 ctgggacaca atggtggtca ggccagtggg aatgtgaacg gtacatcagg taaaggtggc
      241 ccttcatcag gtggtggtac ggatccaaac agcgggccgg gctggggtac gacgcataca
      301 cctaacgggg atattcataa ctataacccg ggggagtttg gtaacggcgg gagtaaaccc
      361 ggtggtaatg gcggtaacag cggcaatcat agcgggagct ctggtggcgg acagtcttcg
      421 gccaccgcga tggccttcgg tctgccggcc ttggccactc cgggggctga aggactggct
      481 ttatccgttt ccggcgatgc gttgtcggcc gctgttgctg atgtgctggc tgccctgaaa
      541 ggtccgttta agtttggtct gtgggggatt gcgatttacg gagtgctgcc ttctgagata
      601 gcaaaagatg atccgaaaat gatgtcaaaa atcgtgacgt cattgccggc agacacggta
      661 acggagacgc cggtcagctc cctgccgctg gaccaggcga cggtcagcgt cactaaacgt
      721 gtggcggata ttgtgaagga cgagcggcag catattgcgg ttgtcaccgg ccggccaatg
      781 agtgttcctg tggtggatgc gaaaccgaca aaacgtccgg gggtattcag tgtgtcgatt
      841 ccgggtctcc cgtctctgca ggtgagcgta cctaaaggtg ttccggccgc gaaagccccg
      901 ccaaaaggca ttattgctga aaaaggtgat tcacgtccgg ctggttttac ggccggtggt
      961 aactcccgtg aagccgttat tcgtttcccg aaagagagcg gacagaaacc ggtctatgtg
     1021 tcggtgacgg atgttcttac tccggcacag gtaaaacagc gtctggagga agaaaagcgt
     1081 cgccagcagg catgggacgc tgctcacccg gaagaggggc tgaaaagaga gtatgataaa
     1141 gcgaaagctg agctggatgc tgaagataaa aatattgcga ccttaaacag tcgcattgca
     1201 tcgacagaga aggcgatccc cggtgcaagg gctgctgttc aggaagccga taaaaaggta
     1261 aaagaggcag aggcaaataa agatgatttt gtgacttata acccgcctca tgaatatggc
     1321 tccgggtggc aggatcaggt tcgctatctt gataaggata ttcagaatca gaatgagaaa
     1381 ttaaaagctg cccagacatc tttaaacgaa atgaatgaat ccttatccag ggataaggct
     1441 gcgctttccg gggcgatgga gagccggaaa caaaaggaga aaaaagcgaa ggatgcagaa
     1501 aataaattaa atgaggaaaa gaaaaaacct cgtaagggaa ctaaagatta cggccatgat
     1561 tattttcctg atcccaagac tgaagatatt aaggggttgg gagagttgaa agagggtaaa
     1621 cctaaaaccc ctaaacaagg tggtggtggt aagcgggccc gatggtatgg tgataaaaag
     1681 cgtaaaattt atgaatggga ttcccagcac ggtgagcttg aagggtaccg cgccagtgat
     1741 ggcgaacacc tcggggcatt cgatccaaaa acgggtaagc aggttaaagg gccggatcca
     1801 aaacgaaaca ttaaaaaata tctttaagag gtaaatatgg ggcttaaatt acatattcat
     1861 tggtttgata agaaaaccga agagtttaaa ggtggtgaat actcaaaaga cttcggtgat
     1921 gatggttctg tcattgaaag tctggggatg cctttaaagg ataatattaa taatggttgg
     1981 tttgatgttg aaaaatcatg ggtttcgata ttacagccac actttaaaaa tgtaatcgat
     2041 attagtaaat ttgattactt tgtatccttt gtttaccggg atggtaactg gtaagcaaaa
     2101 gttatcaagg atgatttgaa atacagactt ccggtgttca gggatgaata ccggggtttt
     2161 taaatacagg gaaggtatat gaaaaaggta aaggttgttt ttctttttat attgattttt
     2221 tcaggctttc ttctggtcgc atgtcaggca aactatatcc gggatgttca gggtggaacg
     2281 gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg ttcagtagaa aagatcaaag
     2341 gatcttcttg agatcctttt tttctgcgcg taatcttttg ccctgtaaac gaaaaaacca
     2401 cctggggagg tggtttgatc gaaggttaag tcagttgggg aactgcttaa ccgtggtaac
     2461 tggctttcgc agagcacagc aaccaaatct gtccttccag tgtagccgga ctttggcgca
     2521 cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc cagttaccaa
     2581 tggctgctgc cagtggcgtt ttgcgtgctt ttccgggttg gactcaagtg aacagttacc
     2641 ggataaggcg cagcagtcgg gctgaacggg gggttcttgc ttacagccca gcttggagcg
     2701 aacgacctac accgagccga gataccagag tgtgagctat gagaaagcgc cacacttccc
     2761 gcaagggaga aaggcggaac aggtatccgg gaaacggcag ggttggaaca ggagagcgca
     2821 agagggagcg attcatcgga aacggtgatg atctttaagt cctgtcgggt ttcgccactc
     2881 ctgactgatt catggttgag ccaccggctc ccacagatgc accgaaaaag cgtctgttta
     2941 tgtgaactca gtcaggaggg cggagcctat ggaaaaacgc cacctgcgtg gccttgccgt
     3001 tttgcctcac atgttagtcc cctgcttatt cacggaatct gtgggtaact ttgcatgtgt
     3061 ccgcagcgcc cgccgcagtc tcacggccgg agcgtagcga ccgagtgagc gaggaggcgc
     3121 aatttccccg gcacctacaa gacggacacc ggacgcgctg atgtggcata ttgtcccctg
     3181 attaagtctt tatattccct gataataaat taccaggata ggtgaatcat atcggttttt
     3241 cgctgaacat tttacgttaa cctatctctg ttgctttttt ctccttacgg agaaatacag
     3301 gtcatccccg cgagggcgtg gctgacctca gggggatatc ccctgcttac gctggctgga
     3361 cgccaccgac gcacggtctc tccccctctt tctggctccg tccggctgga cgccggccac
     3421 gccagaaatt cacccggccc ccgtcaggct cacgcctgac cacccacaaa aatccacggt
     3481 gctggacgca cctggcgaag cagcaaagct gctcctgatt ttgcgggtgg tcagccgctc
     3541 gctatggcga actctgacgg gggccggaac accggaaaag accgcactgg ggtgccgcct
     3601 tttcctctca cacgctgggg cgtgctcgtt gttcctccgg ggctgcgcct tttccgcatg
     3661 gggcggaaag aggctggcct ccgtggtgaa agctgaacgc aatcaccacg gacgtgaccg
     3721 ccgccgccat gccttggggc atgtccgcgg cttgtgcagc gggggctgca catcacgccg
     3781 gcaaaccatc gtctgaagga gagagaacat gtttaatacg gattcgcttg cctggaagtg
     3841 gcacattccg gagctggccc ggcagattta tccgtgggtc agctggctgt accaggagta
     3901 ttacggtctg gccggggctt tcctagttat cggcctgttt ttcggtctgg ctgaactttg
     3961 ggccggacgg atgcgttttg tggggctgag tcataaggag cgacgggagt atgccgatcg
     4021 ctataagcgc gataataaac ggccagcgcc attttcggtg ctggcctcgc tggattcgcc
     4081 cgggcggatg gtcagggcac gtcgacatgt ctggaactac gcgtactggc tgaccggcgc
     4141 ggccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     4201 aaccgtcctg cgtccggacc tctacgcaaa cgcagattat tacctgctgc agccggctaa
     4261 cctgttcggg attgcctggt gcgcgggcgg gctggctgtc gggattgtgt tggcgcggct
     4321 gctggcgttc ctgctgcttg acggtcattt tgcggctgct gacgaggcgg tgaacgcgaa
     4381 actgaatcag gagagccggc gcagcagcca gcggacagga gagatgaccg acgtgcgcca
     4441 cctgcatttc ggggagccgg taccggttaa tgcgctggcc gatttttcct cggagcaggc
     4501 ccggcaggag caggcggtgt ttctgggtaa ggatgagcag ggcaagccgg tgctggttcc
     4561 gcgtgatacc tggcgcaaga ccaacattca gatcctcggc ctgcccggga gcggcaagag
     4621 cgtgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg cggtggtgta
     4681 cttcgacccc aaaggggacg catgggcgcc gcacgtcttc cgggcgcact gtccggactt
     4741 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     4801 ccagtacgcg ctgaaaaacc tgctgattgc cgggcttaac ctgggcgaga ccggcgacgt
     4861 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagtttcc
     4921 gcagggggca aacattcagc aggtgctggc ggcggcgtac gcgctgccgg aggccctgaa
     4981 aaaggacgtg aaggggttga tcactaggct ggaaaacgtc gctgacctga gcgtcctgca
     5041 gacggacagt ggcatcgatg tggcggggat tatcaacggc ggcggctgcc tgtacgttat
     5101 cggctcgatg gacgacgagg cggtgatccg tgtgcaaaaa atgctgtttg cccgctgcgc
     5161 gcaaatcatc attgcgcggg acgagttccg ggagtggcca cacgtcagcg tgatgctcga
     5221 tgaaattaaa tatctgctgt cgaagtatgt actgaacgca ctggggacgc tgcgcagccg
     5281 ggactgcaat ctgttgctcg cgcaccagtc gctgggcgat ttcgggcagt gcgggcagga
     5341 tctgcccgct gaatttgtga aaaccacggt actggataac acgccgatcc gctggtttta
     5401 ccgggcggcc agcctggagt ccgcgcagtg ggcggcgggg cagaccgggg aaatcagggt
     5461 ggacgtggag cgccgccgtg ccagccggga ggcggggaac gtggagcata tcagcgggga
     5521 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc
     5581 ggacgggttc gcggtcatga ccgggctggg tgtggcccgg atggcgttca gcagtccgct
     5641 gcgggttgag cgccgggaaa tcccgttgca gcgtttcccg gtgctggcga aaaccgatcc
     5701 cctggcggag ttccggcccc cggccgacgg cggcgggccg aaagcggatg atgattttgc
     5761 ggggatttac tgatggcgct ggagctgtac aacgtcagcc acgcgaaacg acaggcgcgg
     5821 aacgcggaga aaacccggct gaccctgcgc tggttgcggg aagagctgtg ttccacggcg
     5881 gagctggttg cgcggcggct ggggattgcg gcggtgcagc cggtctaccg cttcctcgac
     5941 agcctggtgg cgaaagggct gctggtccgg accaaatatc cggtggacgg ccgtcaggtc
     6001 agcgtgtggg ggctgacgcc gcatggcgtg gcgttctcgt tcgacgagga cgaaccgctg
     6061 gcggatgtta ttccgtttca gccgtcaagg gtgtcggcag cacatctgcc gcaccggctg
     6121 gccgtgcaat ccctgcgcct tgatatggaa gccagagggg ccaccggctg gcgcattctg
     6181 caccgtctga gtcttaaagg catgaaagtg ccggacgccc tggcggagct ggacggtaaa
     6241 acggtggcgt ttgaggtgga gcgcaccgtc aagagccgtc gccggtatca ggaagtggtg
     6301 tccgggtatc tgttcaaccg caaagctaac ggcatcgatg aaatctggta catctgcccg
     6361 gaccgggcga cgcaggtcag ggtgcagcgg gcaatcctgt cggtggatga aatcgtgaac
     6421 ccacagaccg gcgaggcccg caaaacggca gagctggatc gtgaacggtt gtttgcgtgc
     6481 tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaagac
     6541 gcggaggacc ggaacggcga gcgctgcatc atgccgacca gcgagcatct ggaagatatg
     6601 gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat
     6661 ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc
     6721 acgtactttt tcactggtaa aaatccgcaa tgggaacgga acaaacccac aggaatgaag
     6781 ctgtgtgatt acctctatcg caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg
     6841 gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgacgt ggtgagtgag
     6901 cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac
     6961 agccgctatt ttaacgtgcc ggtacctgaa ctgccggagg acaaacctgc ccgccggtgg
     7021 tggccgttcg gcaaaaaata aatgccagca ccggccatgc cggtgctgtt attcctcccc
     7081 gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgtagcc gctcgcacat
     7141 gtcggccggg gtatccatat ccagctcatt cagtttatcg agcaatagca gcgactgctc
     7201 cttaataaat ttagccatat tgagggctgt tttttcctgt tttgtcatga ttctttcctt
     7261 ccgggcatgg tgtgcgcctg tcaggtatac tgtctcaaaa atagcggggt gagacaatgg
     7321 cgtgggtgac ggtgagacag gcgacggaac tgacgggtaa agcccgaagt tccctgtatc
     7381 gggatatggc gaaaggccgc gtatcatacc gtacggaagc tgatggcggc cgggtggtgg
     7441 ataccagcga gcttatccgg gtgtacggtg agttaagaca ggatgagact cacgagcggg
     7501 acgagttgag actgtcgggt gagactgaaa aaatggcctc cgacgcgttg atcgcggaga
     7561 taaaagcact cagggaggag gtggccgggc tacggcagga aatgcagtcc atgagactgc
     7621 tggaacacaa accggagaca gcagagaccc cggccggaac accgcgccgg tggtggccgt
     7681 ggggaaaacg ctgacgcgct gtgcttgtcc aaccggcgac ggccacgcaa tgaattgcgt
     7741 ccccgtcacc ggttattaga gaacaatatg gggattctgg cgctggccga ccacggccag
     7801 aacccggact tttctggtct ggtcatcaac atcatagagg acatgctgtc ccatcagact
     7861 gatccggcga acgtcctcgc catagcgtga gtccacttca tagagtcgcg ggaagtcggc
     7921 cagtttgtcc gccgcagcga gaacccggtc ggtaacgttc tgcgcatccc acagggatga
     7981 attttccgcg atatagtcaa acagcgtcgt tacatcctcg atggctccct gcttccattc
     8041 cacgtcatac ggcatcgcgc agttcctgat aacggccatg caggcgctgg cggagttgtt
     8101 ccgtggtcat acccggacgc gtgtcagcac gggcggcggt cactttggcg cgaacctgat
     8161 cttcatactc tgcctgggcc agttcttcgt tagccagtcg ctgcaacagc gcagctttgt
     8221 ccgggccacc ttccggcagt atgcggggca ggctttcagc cagttcccga agaaagttgg
     8281 cgttttcgta gtgtgtagtg ttgctcattg cggagcctcc cgggttaatg cggcaatatg
     8341 aaggaagttt agcagggggc ggtctgaaag aatatggtcg tgtcagtcag gctgatgaag
     8401 cagaaagtcg gtaaaaaagg gcttacggca tccattttac gtcaaaacat atgccatgca
     8461 gttgcgtgca taggcatgca ttaggataaa atttatcggg cgcgtttccg gcagttttcc
     8521 gggggggttg ttgcctgttt tatcccgtag ccgccggaaa cgccctgagc ccgtctgagc
     8581 ggtgcgcgta atgacgcgtt atggtaaatc cacttactgt ctgccttcat agccatcgag
     8641 ataaacagct gaaatggtgt cagccagtag ccttgatttt ttcggtgtga ggtctttgct
     8701 gcggtaatcg gtacccgcaa attgtgttgt gccttggagt gagtttaaaa attcctgact
     8761 gtgaaactgc tgaatgcgtt gctgcacatc actccgtgat tttccggtcg ctttatcctt
     8821 tgcgcccata tcccagaact gccggaaaaa aggcaccatc attcgtgttg tgtcagcttt
     8881 ctgggtggaa aaattgggat caaaaccgtc atctgaagca tatacgtaat gccgggtatg
     8941 ctgttccacg ctgccctttg gagcacaggc agtgagagtt gctgtcgctg ttatcatggc
     9001 gataagacac agtttgctgc cggaacgtct catgagcgtc tccttacctt taattctttt
     9061 ttctcattga gttatatatc cgcaggctat cttatcatca gtttctgacc gtgttcagtg
     9121 accgaacaac ccgaaccaga ccccccgttt cttttcctgt cccggagggg gctttctggt
     9181 atctcatata atgatttcag taaaacatac tgaggccgga gaacggcgcc gtttgtgctg
     9241 gtttttgttg catctggagg ggattgcgca tggtgtgagt ggctgttttt ttga
//
