LOCUS       CP083827                3030 bp    DNA     circular BCT 18-APR-2022
DEFINITION  Enterobacter ludwigii strain 11894-yvys plasmid p11894_3, complete
            sequence.
ACCESSION   CP083827
VERSION     CP083827.1
DBLINK      BioProject: PRJNA762162
            BioSample: SAMN21379555
KEYWORDS    .
SOURCE      Enterobacter ludwigii
  ORGANISM  Enterobacter ludwigii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents, 106573 (2022) In press
   PUBMED   35307563
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/21/2021 15:26:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,687
            CDSs (total)                      :: 4,568
            Genes (coding)                    :: 4,439
            CDSs (with protein)               :: 4,439
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 33 of 129
            Pseudo Genes (incomplete)         :: 93 of 129
            Pseudo Genes (internal stop)      :: 27 of 129
            Pseudo Genes (multiple problems)  :: 21 of 129
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Enterobacter ludwigii"
                     /mol_type="genomic DNA"
                     /strain="11894-yvys"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:299767"
                     /plasmid="p11894_3"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2011-10"
                     /collected_by="Yushan Cui"
     gene            858..1085
                     /locus_tag="LCD46_23425"
     CDS             858..1085
                     /locus_tag="LCD46_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOY73162.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAFMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(1133..1321)
                     /locus_tag="LCD46_23430"
     CDS             complement(1133..1321)
                     /locus_tag="LCD46_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UOY73163.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDMLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2337..2849
                     /locus_tag="LCD46_23435"
     CDS             2337..2849
                     /locus_tag="LCD46_23435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOY73161.1"
                     /translation="MTKVTVDYPSSISRRKLSNLFNHSPFMLSLVHDMCDSQAIVLAAM
                     CEGKCVTSAGNRIEADYEVTKLAAVIDVLENKFYLPVSRVKIPTASDTGGGTIQAKYLI
                     TENDMQLLLEDPESVVLTRERLALSKLKSRDERCLKRLVSVHGYDEVFRSLQALDVAND
                     SFGRDCG"
                     /name="hypothetical protein"
     Typing          507..680
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.571"
                     /coverage="100.0"
                     /evalue="1.23e-64"
     Typing          complement(38..583)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.364"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tatggctatc acatcaagac tcatcccttt gcccagttac caatggccgc tgccattggc
       61 gctttgccgt gtctatccgg attggattca agtcgaaagt taccggaaca ggcgcagcgg
      121 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcccggaa
      181 ccgagtgtca ggcgtggaat gagaaaccgc ggccataaca gcggagtgac accggtaaac
      241 cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt
      301 taagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
      361 gggggcggag cctatggaaa aacggcaacg ccgtggcctt gcttcttctt tgctaagtat
      421 cgccctggca tcctctagga aatttccgcc cagtttgtaa gcccacaccg ctcgccgcag
      481 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaatatctcc tgtctcgtat
      541 attctgctga cgcgccggtg ctgccttttt cctccttcca catgaagcac ttcactgacc
      601 tccgcatccg tgtcgacata ttaagccagt atacactccg ctagcgctac gtgactggtt
      661 cagggctgcg ccccgacacc cgctaaaagc actgacgcgc ctgcggcttg cccaaccccg
      721 cgccgtccgg gaaagaattt cccgatcgac ccgacgtttc tgtttacctg tgatgttaac
      781 gtttggatct ttttcccggg tttacctatt ttcttctaat gaagcgttca cagctgacgt
      841 taatagatgg gtgcttcatg tttggttttt caccaggaga acttatgaac ctttgccccg
      901 atgaacgtct gctctttgtg cggatgattt ccgctatgct tcgccgttcg ggcggtgatg
      961 cgggtgcttt tatgtttgag gcgtatcgcc atattgtgtc cgataccaat caagctcgtc
     1021 gttcctacat gctcgacctg ctcgaaagcg tcaggcatga ctatgtacac ggtggatata
     1081 catagttgcc ctggatacgc ttttcgtcta agtgagcgtt cttttcgtct ctttattcgc
     1141 cgtcctgtat accgaagcgt gcgctcagct tctgataaag cgcttcagcc tgttcgtgca
     1201 ttcgctcaca gtcggctgcc tcgtcatcaa gatcgagcat gtcgagtttt tcgagcagca
     1261 gcagcgactg cgcccggata aattttgcca tgttcagtgc ggctgattcc tgttttgtca
     1321 tacggctttt tcccggtttt atggtgatcg cagactgaaa tcttacttca ttcacttgag
     1381 tgcatagcca tgcaggtacg cagaaatcat caggggcgtt ttttctcgat atgggcacag
     1441 aaaggtgtat gtttcaggcc ggaggcctca gaaacgccct gagagcctct gaggcggtgc
     1501 gggcaacgga tgttatgtta aaagttactt tcccgagctt gtgtcgtagt ttcttcctac
     1561 gtgattcatc aagtaggaac ctgatagatc gatacaccag agatcctcat cgatccttct
     1621 ttatttattt aatttattgt taaacaaaga ctatttgtaa acctataaac tacaccgatc
     1681 gattgggatc ctttcaatag gaaaaaacac taaacaattt atatatgctc taaacggctt
     1741 caaactgatt taaacggctt taaattattg gtgaacctat gactaggttt ttgtgtagat
     1801 aatgtcttag gcagcgtttg gtgccgtaca gtgggggttt tacaggctgc tctcgatgct
     1861 gctgttggtc tgatggctgg ttttttaaac actgtttacg ggatccctcg ttagaattac
     1921 tatccatggc gctggctgtg gtggctggca attaaaaaac ataggcaggt agctgtggtg
     1981 gctatttgtc gaagcttgag atggcttctt aagaaggtga aaccttcaca agagaacccg
     2041 ctaatcgtga agaggcgggt ttttttgtgt caaaaatcct cgattcatca gcgcattaat
     2101 tgctcttcaa tatggtgatc gtgttagaat aggcgcaacg gcagacctta ccagtttgcg
     2161 agccttacca gctcatgccc cgttagcaaa taaccttaat cttcttcacg gaccgcaagg
     2221 tcaaggtgcc aaaatgtgta atacaatctc gatccctcat ccgggggatg ttagctatat
     2281 ctccttcacg tgccgcaagg ttccgtccgc tatctttagt ggcgtggagg tgaatcatga
     2341 ctaaagtaac tgtcgattat ccttcttcta tctcccgccg taagctttca aatctgttca
     2401 atcactcccc gtttatgttg tctcttgttc atgacatgtg tgactctcaa gctattgttc
     2461 ttgctgccat gtgtgaaggg aagtgcgtaa caagtgcggg gaaccggatt gaagcagact
     2521 acgaagtaac taagctggct gcggtcattg atgttctgga aaataagttt tacctgcctg
     2581 tatcacgcgt gaagattccc accgcgtcgg atactggagg tggaactatc caagctaaat
     2641 atctcattac ggaaaatgat atgcagctgt tgctggaaga tccggaatca gtggttttga
     2701 cgcgagagag attggctctt tctaagttaa aaagtcgaga tgaacgttgt ttaaaacgtt
     2761 tggtgtccgt tcacggttat gatgaagttt ttcgatcact acaggcgtta gatgtagcta
     2821 atgattcttt tggtcgagat tgcgggtaat gctttaaacg gcaatgaatt gacgtttaaa
     2881 gcatggccgt ttaacgtttg acgttagaaa gaaaaaagcc acccggcaag gtggcttttt
     2941 caaggttcaa caaacgggca agtttttgaa ccgggtaact gtcgtaaggg cgacgagtca
     3001 ccaaaacaag tatctccagt ttagccgcaa
//
