LOCUS       CP083826                4935 bp    DNA     circular BCT 18-APR-2022
DEFINITION  Enterobacter ludwigii strain 11894-yvys plasmid p11894_2, complete
            sequence.
ACCESSION   CP083826
VERSION     CP083826.1
DBLINK      BioProject: PRJNA762162
            BioSample: SAMN21379555
KEYWORDS    .
SOURCE      Enterobacter ludwigii
  ORGANISM  Enterobacter ludwigii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Liao,W., Cui,Y., Quan,J., Zhao,D., Han,X., Shi,Q., Wang,Q.,
            Jiang,Y., Du,X., Li,X. and Yu,Y.
  TITLE     High prevalence of colistin resistance and mcr-9/10 genes in
            Enterobacter spp. in a tertiary hospital over a decade
  JOURNAL   Int J Antimicrob Agents, 106573 (2022) In press
   PUBMED   35307563
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Liao,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2021) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/21/2021 15:26:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,687
            CDSs (total)                      :: 4,568
            Genes (coding)                    :: 4,439
            CDSs (with protein)               :: 4,439
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 33 of 129
            Pseudo Genes (incomplete)         :: 93 of 129
            Pseudo Genes (internal stop)      :: 27 of 129
            Pseudo Genes (multiple problems)  :: 21 of 129
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterobacter ludwigii"
                     /mol_type="genomic DNA"
                     /strain="11894-yvys"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:299767"
                     /plasmid="p11894_2"
                     /geo_loc_name="China"
                     /lat_lon="30.15 N 120.10 E"
                     /collection_date="2011-10"
                     /collected_by="Yushan Cui"
     gene            join(4676..4935,1..232)
                     /locus_tag="LCD46_23400"
     CDS             join(4676..4935,1..232)
                     /locus_tag="LCD46_23400"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UOY73158.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            239..454
                     /locus_tag="LCD46_23405"
     CDS             239..454
                     /locus_tag="LCD46_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UOY73159.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1382..2668
                     /locus_tag="LCD46_23410"
     CDS             1382..2668
                     /locus_tag="LCD46_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="UOY73160.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWERLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            3675..3998
                     /locus_tag="LCD46_23415"
     CDS             3675..3998
                     /locus_tag="LCD46_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOY73157.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKSARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3988..4701
                     /locus_tag="LCD46_23420"
                     /pseudo=""
     CDS             3988..4701
                     /locus_tag="LCD46_23420"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3390..3562
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          complement(2919..3462)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.698"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2..552
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.701"
                     /coverage="100.0"
                     /evalue="2.78e-93"
     Typing          3989..4935
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.418"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgatcgcc acgagcggga gcattctgtg gtggcagggg cagcagataa ccgacaatta
       61 cacgcatctt cgtcagcagg aagataccct ggcgaaaatg accgcccgga cgtggggagt
      121 gcgctatcag gagagcagcg acggccggag gtttcttatc ctgccgccgg ggatgcaagc
      181 cgaagccatc ccctacgacg ggacgacatg gatccgcctg aaacaggagt agcggaaaat
      241 gacagagctg gaaaagcagt tgctgagcgc attagagcag ctacagcagg actactcgca
      301 aaggctggac gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg gtcttatgca
      361 acgggagaac gcggtgctga acgagcgcgt gtcggacttg agcacgcagg tgctgagttt
      421 aagcgagcag ctgcgccgct tgtccgggaa ctgaatgcca tcgaggagaa ggctgcactg
      481 gagtatcagc gtcagaacga gctggagctg acgctccgac agaagcagaa gagtttcgat
      541 ggtccgaaga tgaggtgaca gcccgcacga agaagggctc atcgcgttcc tgctaaatag
      601 gctgactacc ggaatggtag tgacttatcc cggaggaagt gatgaaaatt ctcaacgtta
      661 ttgaagccct gctgaactgt ttttacctgg tgatggagct gtgtgagttg ccggagaggc
      721 atttgccggt ttttaaggcg ttcgcgctga ttttagtgtg actttacgct gcatgcatct
      781 ggctgcatga ctatgcatca gtgtaatatc cagcggctgc gtttttggtg ttttttcgtc
      841 atgtctgtcg ccactttctc gcgtctggca ccggaaacgc agccaacccc gtttctgcgg
      901 tgcgggcaag gggtgttatg ttaaaagccg aggtctggcg gatacggaaa aaattatgcc
      961 ggccgggctt atgcccgcgg aaatccggcc gatacttaaa ttatccttca caaaggatat
     1021 cctgcgatcc agtcagttga gtgttttccg tctcccggat gggagccatt tttttgcctt
     1081 ctgatgcgtg ttaaggcttg cggagctgcg ctggttcttc caagattgag agacgccagc
     1141 ttcctgctgg agccggcgga tttgccgtaa atatgaacat tttcttattg ttcagcccgc
     1201 taaagatggc gggtttttta cgcgtgaaat ccggagccgg acatcatgcc ggcatggctg
     1261 aagagcgggc tttttcccgc cctgtcgcag taatatataa agctacataa ggagtagccg
     1321 ataaccatgg cagtacttac attatccggg caatctgatt catatgtttt cacttaaata
     1381 tatgttctcc ctgaacctcc agcgatcagc catttcgtat gacgcaattt gtcagaataa
     1441 gcctgtcatt gagttcagtc cggagggggt gataaataaa gccagcccgc tttttctctc
     1501 cactatgggg tacagggctg atgaaataat cggtcaccac catcgtatat tctgcccgcc
     1561 ctcgctggta agttcaccgg aatacagcca gttctggcag cgccttgcca ggggagaaag
     1621 ctttagtggc aagtacctgc ggctggcaaa gggaaatcgt tccgtatggc ttgaagccag
     1681 ttatatcccc gtttctgaca ggcgcggccg tgttatcaga gtcattaaaa ttgctgccga
     1741 tatttctgag cgcgtgcatt ctgctcttga gcaggaagca gtcgtaaatg ccataaaccg
     1801 ttctatggcc atcatcacct tcaatcctga agggatcgtg cttgaagcaa atgaaaattt
     1861 cgtcaatgcc actggctata agcgggatga aatcataggt aagcatcatc gtctgttctg
     1921 tgccgagacg ctttacaaaa gtgatgaata ccgacatttc tgggagagac tgaatcaagg
     1981 tgagtttttt tctggtctgt ttccacgcct taaccgtcag ggagaccctc tgtggttccg
     2041 tgcaacttat aaccctgtct ttaacagtga tgggcagctt tataaaattg tgaaattcgc
     2101 tacagatgtt acggctgatg tcctgcgaaa ccagagagag caagaggctg ctgtgcatgc
     2161 atgggatatg gccgtgcaga cccgagaaag tgcgcagaac ggtgccaatg ttattgaaaa
     2221 tagcatcctt atgattgaca gaattgcgca ggggatgggg gctgtctcca ccgatatctc
     2281 acggctcaac aatcagtctg aaagtattga cgatatggtg gaaaccatcc ggaagtttgc
     2341 catgcagaca agacttattg cactgaatgc cgcgattgaa gcagcaaggg ctggcgcatc
     2401 cggaagaagt tttgcggttg ttgccgcaga agtgcgtaat cttgcggcga gcgtcagtag
     2461 cgcgactgaa gaaattgagc aagtcgtggc cagcaatagt caactggcca aggatgttct
     2521 ctgtggcatt gaaaacagcc tgatgaacac tcggaaaggg gttacactca tgcgcgaagc
     2581 gggtgaagtt atgaccagca ttcagaggaa tgctgcaggg gttgagactg cggttaagga
     2641 tgtcgccatt tcagttaaag ccggataggc catggtgcat atccccatat ctgtcgaaca
     2701 tcacagatga tcttcttgag atcctttccg ttgcgtcgta atctcttgct ctgaaaacga
     2761 aaaaaccacc cggcaaggtg gctttttcga aggttcgcaa gagttgacgc tcttttgaac
     2821 cgcggtaact ggcttggagg agcacagtca ccaaaactgt cctttcagtg tagccttatc
     2881 gcacccgaaa cttcaagact ctcctcctct aaacctgtta ccagtggctg ctgccagtgg
     2941 cgctttgtcg tgtttttccg gattggactc aagttgatag ttaccggata aggcgcagcg
     3001 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg ccttcccgga
     3061 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     3121 accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc
     3181 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     3241 caggggggcg gagcctatgg aaaaacggct tcgccgtggc cttatcgctt ccctgttaag
     3301 cttctttctg gcatctccca ggaaatttcc gccccgcctg taagccggta ccgctcgccg
     3361 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaatata tcctgtatca
     3421 catattctgc tgacgctccg ctgctgcatt tttctcctgc cagatgaagc acttcactga
     3481 tattcgcatc cgtgccaata tagtaagcca gtatacactc cgctagcgct acgtgactgg
     3541 ttcagggctg cgccccgaaa cccgccaaaa gcctgacgcg cctgcggctt gtccaacccc
     3601 gcgccgaccg ggaaaggctt tcccgcccgt cccggcgtta tcaggaggcc ggtttgtcag
     3661 agaaacggaa caagatgctc accatgtggg tgacggagga cgagcaccgg cggctgcttg
     3721 agcgctgtga cggcaggcag ctggcggcgt ggatgcggca gacctgcctg gacgaaaagt
     3781 ctgcgcgcac cggaaagctc ccctcaatct cgccggcgct gctgcgccag cttgccggca
     3841 tggggaataa cctgaaccag atagcccgcc gtgttaacgc cggcggcggc accggccatg
     3901 acagggtgca gattgtggcg gcgctgatgg ccatcgatgc cgggcttgag cggctgcggc
     3961 acgccgttct tgagaaagga acggacgatg atcgttaaat ttcatccacg tggccgcggc
     4021 ggtggcgccg ggccggtgga ctatctgctg ggtaaagacc ggcagcgtga aggcgccagc
     4081 gtcctgcagg ggaagccgga agaagtccgg gagctgattg acgcgtcgcc ttacgtgaag
     4141 aaatacacct ccggcgtcct gtcgtttgcg gaggccgatc tgcctcccgg ccagcgggaa
     4201 aaactgatgg ccagcttcga gcgggttctg atgcccggac ttgataaaga tcagtacagc
     4261 attctgtggg tggagcacga ggacaagggg cgtctggagc tgaactttct gatccccaat
     4321 accgagctgc tgaccggcag gcggctccag ccgtactacg accgcgccga ccggccgcgt
     4381 atcgatgcct ggcagaccat cgtgaacggc agactggggc tgcacgaccc gaacgcgccg
     4441 gaaaaccgcc gggcgctggt cacgccgtcc ggactgccga aaacgaagca ggaggccgca
     4501 gaagccatca cgcgcggact gctgatcctt gcttcgtccg gggagctgaa aacccgtcag
     4561 gacgtcactg aggcgctgga acggtcaggt tttgaggtgg tacgcaccac gaaaagcagt
     4621 atcagcattg ccgacccgga cggggggcga aacatccgac tgaagggagc catctatgag
     4681 cagtctttta acgctggcga aggacttaga gcagcaatcg aaagcgcagc agaagagtac
     4741 cggcgagatg ctgaaagccg cattcagcga gcacgagcag tctgtcagag cggaactgaa
     4801 cgcaagcgcg aggagaatca gcgacgccat cagcgcccac gagcagagta tgagcgaggc
     4861 gatggagaaa aaccggcgga gcgtgttgct gacggccggg aggacatggc tcaccatcct
     4921 gatggtgtca gcgct
//
