LOCUS       CP083491                6200 bp    DNA     circular BCT 10-NOV-2021
DEFINITION  Escherichia coli strain elppa8 plasmid unnamed1, complete sequence.
ACCESSION   CP083491
VERSION     CP083491.1
DBLINK      BioProject: PRJNA610462
            BioSample: SAMN21169131
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Huang,Y.T.
  TITLE     Genome sequencing and analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Huang,Y.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) Department of Computer Science and
            Information Engineering, National Chung Cheng University, No.168,
            Sec. 1, University Rd., Min-Hsiung Town, Chiayi City 621, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/16/2021 10:07:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,080
            Genes (coding)                    :: 4,671
            CDSs (with protein)               :: 4,671
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 409
            CDSs (without protein)            :: 409
            Pseudo Genes (ambiguous residues) :: 0 of 409
            Pseudo Genes (frameshifted)       :: 85 of 409
            Pseudo Genes (incomplete)         :: 164 of 409
            Pseudo Genes (internal stop)      :: 216 of 409
            Pseudo Genes (multiple problems)  :: 51 of 409
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="elppa8"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24 N 121 E"
                     /collection_date="2019-04"
                     /collected_by="National Chung Cheng University"
     gene            1093..1242
                     /locus_tag="K9U11_00005"
     CDS             1093..1242
                     /locus_tag="K9U11_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UEO18504.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(1987..2427)
                     /locus_tag="K9U11_00010"
     CDS             complement(1987..2427)
                     /locus_tag="K9U11_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UEO18505.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            2645..3034
                     /locus_tag="K9U11_00015"
     CDS             2645..3034
                     /locus_tag="K9U11_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UEO18506.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGDYD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3078..3482
                     /locus_tag="K9U11_00020"
                     /pseudo=""
     CDS             3078..3482
                     /locus_tag="K9U11_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(3589..4425)
                     /locus_tag="K9U11_00025"
     CDS             complement(3589..4425)
                     /locus_tag="K9U11_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UEO18501.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(4425..5228)
                     /gene="aph(3'')-Ib"
                     /locus_tag="K9U11_00030"
     ARG             complement(4429..5228)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            complement(5289..6104)
                     /gene="sul2"
                     /locus_tag="K9U11_00035"
     ARG             complement(5293..6104)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3593..4425)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          885..1058
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(356..961)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtttcatgat gatgtatctg tagcattttg gaaataaata tatcatctct caataagtag
       61 atatatttat cttttttgct tctttggtgc tgcaagtcac caatacgagg gaaacctgtt
      121 acggagcggt ttaaccgtaa tcaacagtga ctggtgtcat gttcggaaga ttaattgata
      181 gtaatctaca aaggatcttc ttgagatcct tttcaatgaa tcgtaatctc ttgctctgta
      241 aacgaaaaaa ccgcctgggg aggcggtttg ttcgaaggtt aagtcagttg gggaactgct
      301 taacccggta acagggttca cgagaccgcc attaccaaaa ctgttctttc agtgtagccg
      361 cagtcaggcc tctacttcaa gacctcttaa tctatcttct cgtgcactcc tgttactaat
      421 ggctgctgcc agtggcgttt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc
      481 ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
      541 aactgccttc acggaactga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg
      601 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg
      661 aaacgcctgg gatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
      721 tttgcgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg tggccttatc
      781 tcttctttgc taagtgtctt cctggtatct tccaggaaat ttccgtcccc gtccgtaagc
      841 cggtaccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
      901 atatctcctg tatcgcatat tctgctgacg cgccggtgct gccttttttc tcctgccaca
      961 tgaagcactt cactgatttt cgcaatcgtg gcaacatagt aagccagtat acactccgct
     1021 agcgctacat gactggttca gggctgcgcc ccgaaacccg ctaacaacct ctcagctact
     1081 ggatatcacc ccatgccatc tgaatatgat gaacaactgg attcgactga tacgcagctt
     1141 cccggcgaat atgacctttt tgatttaatg gcgcaggtta ctgaagagaa cctgcatgcc
     1201 cctgtggatt ttgggtcgcc tgtcggccgg gaagcgttat agctttttcg cttgcccgcg
     1261 tacgtggtaa tactccggta ttacctttta tttctttatt ttcatgtaat tacagtggat
     1321 ttttaatacc cgggtattac tcgtgaaaac gaaatacttc ttttttttct tttaattcat
     1381 gcagttgcga tgaaaatcgc ggctgcgtta ggtgtatgac cattttgagg ttgaaaaata
     1441 atcaaccatg gcgggtgatt tacgcaggtt aaggggagat cgtgacaaaa aaagcgttcc
     1501 tcactgtatc gttcatgcct gcatgaacgg gttattcttc ctgactacag aattacatcg
     1561 ctccttcagt tcatacatca cttttacatc acttatacat cacttttaca tcattccttc
     1621 agctcatacg tcactttcat cctctgtgcc atttgtacat cacccccttc ctgaacgtgc
     1681 tgacagcggg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc
     1741 ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg acttaccccg gctgaatggt
     1801 gtgaatttcc gcgctatgca cttgtgcgca tactcatgca tgccgtaaaa acagagcctg
     1861 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa
     1921 cgccctgagg gcctctcagg cggtgctgat aacagatgtt atggtaaatc agagtcacct
     1981 gcactgttac tctgaaccgg agcgaaacag ccgcgaccag aatccccgct ttttttcctg
     2041 tgccagcgcg tcagccagac ggctaatttc ggcctgtagt tgttccctct cctcatttcg
     2101 caggcggttt tctgcttccc gcgccatctt gtcttcaagc attagggtaa cagtctgctg
     2161 cagagcgtcc agtttctgct gaatgacgtc tagctgagca ttttccgcct ggctaacgac
     2221 agatttggag tgtccctcct tgtgcctttt tggtgtctca gaagtgcgca gctcgccata
     2281 ggcccgaatc agttcactgg tctcgaactc ccggcgatca ttttttcctg tgcgatagct
     2341 gacaaggcct ttagccatgt cacgatataa ttgcgagcgc gactttcctg ttaatttcag
     2401 tgtctcttga actgtgtgcc atgccataaa agtgacagtg tctcctgtgt gtaccgtatt
     2461 gtaacagcag aacatattac tgttttaaca caaaaggtct gaggctgccc gtgaggcgtg
     2521 aaaaatacac aaaaaacctg ttgacggtgt ggttttgacg ggcgtagtat ttcgaatact
     2581 gtatataaac acattgttat aaatacagtt tttgctgttt ttaaaagtca aagaggataa
     2641 ttttatgagt acagagttaa atagcggtac tgtcggtggt tttggtcaca tgggaccaaa
     2701 tgacccctac aatgatggtc atagcggctc tggaagttcg ggaggtagtt ttaacggtgg
     2761 tggtaatggt gactatgata atagtagtgg ttcatcttat actgcatcct ttggtctcat
     2821 cactggccga cacccgataa ctgtcccagg tacgaagaat attaattctg tcgatctttc
     2881 tgctgtcatt aataacatta tacatcatga aggtgcacgc aataacatgt acctagatac
     2941 tgcgaacaat gtaagagtgg gagtgacggg cactggctgg caatgtctag caacggcagg
     3001 catttcggct gagggtaaaa gaactttccg ctaagcgata gactgtatgt aaacacagta
     3061 ttgcaaggac gcggaacatg cctcatgtgg cggccaggac ggccagccgg gatcgggata
     3121 ctggtcgtta ccagagccac cgacccgagc aaacccttct ctatcagatc gttgacgagt
     3181 attacccggc attcgctgcg cttatggcag agcagggaaa ggaattgccg ggctatgtgc
     3241 aacgggaatt tgaagaattt ctccaatgcg ggcggctgga gcatggcttt ctacgggttc
     3301 gctgcgagtc ttgccacgcc gagcacctgg tcgctttcag ctgtaagcgt cgcggtttct
     3361 gcccgagctg tggggcgcgg cggatggccg aaagtgccgc cttgctggtt gatgaagtac
     3421 tgcctgaaca acccatgcgt cagtgggtgt tgagcttccc gtttcagctg cgtttcctgt
     3481 ttggggtcgt ttgcgggaag gggcggaatc ctacgctaag gctttggcca gcgatattct
     3541 ccggtgagat tgatgtgttc ccaggggata ggagaagtcg cttgatatct agtatgacgt
     3601 ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg cgttgctcct cttctccatc
     3661 cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc tggtcgagca ggcgacgcgg
     3721 atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt gcaatgcgtc taggatcgag
     3781 acaaaggtcg tctctgtcag ccggatcgta gaacatattg gcggcgccaa agcccacttc
     3841 accgaccaga ccgacgggat ctatcaccag ccagccgcga ctggagaaca tgatgttttc
     3901 atgatgcaga tcgccatgta gcccacgcag ttccgaggca ttgctcatca tttgatcggc
     3961 tataatcgcc gcgtggacgt agtcagtttg acaacctgcg ttttgatcat cgcgcgcccg
     4021 ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg gcagaaggca ggggttcctc
     4081 agatgcggca tacagcttcg ccattagttc cgctgcaatt tcggtcgcct ggtagtcgcc
     4141 gtgctcggca acgatgtgag agagcattcg ctccccggca tattcgagca acatcagatt
     4201 gttctcacga ccgagcaacc ggactgctcc cctcccattg cgccatacca gatagtcggc
     4261 cccgcgcagt tcatcagcaa tgtcttctat aggtttcaat cccttgacga ttgcaggagt
     4321 cccgtctggc aatgaaactt tccaaacgag gctggaaaag gtgtccgcaa tgagaacagg
     4381 ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg aacatcaacc ccaagtcaga
     4441 gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg gggcttcgat ccccaataca
     4501 ttgaatagga cagcgaaggc gcgctctgct tcatctggcg ctgcccagtt ctcttcggcg
     4561 ttagcaatca tgagtgccaa atcggcatag cgatctgctg ttccgagccg cccaaggtcg
     4621 atcagacccg tgcattgaag agttttaggg tccaccatga agttcggcat gcagggatca
     4681 ccatggcaaa caaccatatc ggtgcgctct tggtcgagcc gcaccggtag ctctcgttcg
     4741 acacgagcca aaagatcgag ctgcggcgta ctcttgtcct cgtccggtaa gaagtcggga
     4801 ttgacggcat tgcgggacac cacatcaacg gcgcgtccga acattcgcga cagcctgcgc
     4861 tcaaacggac attgatcaac cgataggctg tgaacagcgc caagttgctg ccccattgac
     4921 ggccacgctt tgagcaaatc cgctccagac agatcagccg ccggtactcc cggaattgcc
     4981 gttatcacca agcatgcacc ctcctgttcc tcctgccagt tgatgacctc ggggcaagcc
     5041 acacctcgac ctttgagcca aatgaggcgg tcacgctctc cagcgagctc accgcggcgg
     5101 gaagcaggtg cgattttcgc gaaggcatgc ccgtcaccac gtcgaaaaac aaaatcacca
     5161 gattctccgc ctctgacagg caaccagtca gaatgcgatt cacccaaaaa aatattagtt
     5221 cgattcaatg gaggttcctt cagttttctg atgaagcgcg aatatagaga aatatcccga
     5281 atgtgcagtt aacgaattct tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg
     5341 cgcaaggggc gcggctcgtg tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc
     5401 tctgcagcga gtgtcgcggc cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga
     5461 aaggatttgc gcgaaacaga cagaagcacc ggcaaatcga agcgcagccg caattcatcg
     5521 aaccgcgcca gcaccgagag cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg
     5581 ggatcaagga caaggcggtt gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg
     5641 tcaaagaacg ccgcaatgtg atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc
     5701 tgcccgtctt gcaccgaatg cataacgacg agtttggcag atgatttcgc caattgcgga
     5761 tagaacgcag cgtctggaaa accgcgaata tcattgagat aggccacacc acgcgacaag
     5821 gcataggctt gcgtcgcggg ttgataactg tcgagcgaga cgggaatgcc atctgccttg
     5881 agcgcgtcca gcaccggcgc gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg
     5941 tcgggattgc tggatgccgg accgaggtcg atcacatctg ccccctcggc catcagctta
     6001 cgcgcctgcg caatggctgc gtctggcgcc agataccggc ctccatcgga gaaactgtcc
     6061 gaggttatgt tgacgatgcc gaaaatgatg agcgatttat tcatgggggc ttctataata
     6121 ataataatcg agcatgagtc tcatacggat gctcgggtcg aaagggaatc cccaggcgag
     6181 taacctgttt gcggtgatcc
//
