LOCUS       CP082735               11046 bp    DNA     circular BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain CVM
            N16S084 plasmid pN16S084-2, complete sequence.
ACCESSION   CP082735
VERSION     CP082735.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN08113963
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 11046)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 774x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 17:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,642
            CDSs (total)                      :: 4,522
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 53 of 119
            Pseudo Genes (incomplete)         :: 67 of 119
            Pseudo Genes (internal stop)      :: 24 of 119
            Pseudo Genes (multiple problems)  :: 22 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="CVM N16S084"
                     /serovar="Reading"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pN16S084-2"
                     /geo_loc_name="USA:NY"
                     /collection_date="2016"
                     /collected_by="FDA"
     gene            1..2130
                     /locus_tag="CWX46_023150"
     CDS             1..2130
                     /locus_tag="CWX46_023150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAF97836.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            2191..2403
                     /locus_tag="CWX46_023155"
     CDS             2191..2403
                     /locus_tag="CWX46_023155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF97837.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            2405..2611
                     /locus_tag="CWX46_023160"
     CDS             2405..2611
                     /locus_tag="CWX46_023160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAF97838.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            2641..3480
                     /locus_tag="CWX46_023165"
     CDS             2641..3480
                     /locus_tag="CWX46_023165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAF97839.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            3467..4318
                     /locus_tag="CWX46_023170"
     CDS             3467..4318
                     /locus_tag="CWX46_023170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAF97840.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            4627..5442
                     /gene="sul2"
                     /locus_tag="CWX46_023175"
     ARG             4628..5439
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5888..6306
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             5504..6303
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6306..7142
                     /locus_tag="CWX46_023185"
     CDS             6306..7142
                     /locus_tag="CWX46_023185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAF97831.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            7529..7771
                     /locus_tag="CWX46_023190"
     CDS             7529..7771
                     /locus_tag="CWX46_023190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAF97832.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(7803..8429)
                     /gene="tetR(A)"
                     /locus_tag="CWX46_023195"
     CDS             complement(7803..8429)
                     /gene="tetR(A)"
                     /locus_tag="CWX46_023195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAF97833.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLSPLL
                     RAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            8535..9734
                     /gene="tet(A)"
                     /locus_tag="CWX46_023200"
     ARG             8536..9731
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(9665..10102)
                     /locus_tag="CWX46_023205"
                     /pseudo=""
     CDS             complement(9665..10102)
                     /locus_tag="CWX46_023205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(10564..10848)
                     /locus_tag="CWX46_023210"
     CDS             complement(10564..10848)
                     /locus_tag="CWX46_023210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAF97835.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     ARG             6307..7139
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          10803..10958
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          2659..3480
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2..2127
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg
       61 gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg
      121 cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct
      181 gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg
      241 gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgact
      301 ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac
      361 tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg
      421 ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc
      481 aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca
      541 ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc
      601 gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc
      661 cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac
      721 ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag
      781 aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc
      841 cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga
      901 gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg
      961 ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg
     1021 gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct
     1081 gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg
     1141 gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc
     1201 aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg
     1261 atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg
     1321 aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg
     1381 ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct
     1441 gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc
     1501 gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac
     1561 ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac
     1621 aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag
     1681 accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag
     1741 cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc
     1801 cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc
     1861 ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc
     1921 cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc
     1981 aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc
     2041 agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg
     2101 gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac
     2161 tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg
     2221 gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca
     2281 aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc
     2341 tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac
     2401 tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt
     2461 acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg
     2521 gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca
     2581 gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca
     2641 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
     2701 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
     2761 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
     2821 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
     2881 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
     2941 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
     3001 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
     3061 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
     3121 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
     3181 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
     3241 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
     3301 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
     3361 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
     3421 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
     3481 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
     3541 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
     3601 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     3661 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     3721 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     3781 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     3841 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     3901 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     3961 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     4021 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     4081 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     4141 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     4201 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     4261 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     4321 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
     4381 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
     4441 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
     4501 gatgtatgct cttctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
     4561 gattcccttt cgacccgagc atccgtatga tactcatgct cgattattat tattatagaa
     4621 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
     4681 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
     4741 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
     4801 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
     4861 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
     4921 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
     4981 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     5041 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     5101 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     5161 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     5221 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     5281 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     5341 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     5401 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     5461 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     5521 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     5581 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     5641 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     5701 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     5761 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     5821 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     5881 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     5941 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     6001 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     6061 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     6121 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     6181 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     6241 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     6301 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     6361 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     6421 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     6481 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     6541 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     6601 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     6661 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     6721 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     6781 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     6841 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     6901 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     6961 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     7021 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     7081 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     7141 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     7201 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgacccctt tctggcgcgt
     7261 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg
     7321 gaaaggcgct ggaagccccg tagcgacgcg gagaggggcg agacaagcca agggcgcagg
     7381 ctcgatgcgc agcacgacat agccggttct cgcaaggacg agaatttccc tgcggtgccc
     7441 ctcaagtgtc aatgaaagtt tccaacgcga gccattcgcg agagccttga gtccacgcta
     7501 gatctatctc atctgcgcaa ggcagaacgt gaagacggcc gccctggacc tcgcccgcga
     7561 gcgccaggcg cacgaggccg gcgcgcggac ccgcgccacg gcccacgagc ggacgccgca
     7621 gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt ggacgctagg
     7681 gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt ggaaaggggg
     7741 aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct
     7801 gatcaatcgt caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc
     7861 aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg
     7921 aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg
     7981 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     8041 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     8101 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     8161 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     8221 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     8281 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     8341 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     8401 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     8461 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     8521 tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc actgtcgcgc
     8581 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     8641 ttcactcgaa cgacgtcgcc gcccactatg gcattctgct ggcgctgtat gcgttgatgc
     8701 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct
     8761 tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg gcgcctttcc
     8821 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     8881 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     8941 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     9001 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     9061 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     9121 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     9181 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     9241 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     9301 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     9361 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     9421 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     9481 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     9541 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     9601 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     9661 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     9721 gagccgatcg ctgatagagg tttatagcgg cttcgttccc ttccgcaacc gtcagcatga
     9781 tctgctccac atgctcacgg ccatagtcta tcaccgcccg cagcagcata ccggcgaccc
     9841 ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct
     9901 tggggccgtc ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc
     9961 cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt
    10021 gctcgtaaac ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc
    10081 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg tactaactgt
    10141 cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat
    10201 aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa
    10261 aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg
    10321 ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt ctattcgctg
    10381 cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct
    10441 aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct
    10501 ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat
    10561 cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc
    10621 gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt
    10681 caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc
    10741 ttgctgctgt tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc
    10801 ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca
    10861 ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta
    10921 ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc
    10981 ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga
    11041 ggcagc
//
