LOCUS       CP082728               11035 bp    DNA     circular BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Muenchen strain CVM
            N17S129 plasmid pN17S129, complete sequence.
ACCESSION   CP082728
VERSION     CP082728.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN10132778
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Muenchen
  ORGANISM  Salmonella enterica subsp. enterica serovar Muenchen
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11035)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 11035)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAR-2019
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 450x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 15:41:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,734
            CDSs (total)                      :: 4,611
            Genes (coding)                    :: 4,518
            CDSs (with protein)               :: 4,518
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 42 of 93
            Pseudo Genes (incomplete)         :: 60 of 93
            Pseudo Genes (internal stop)      :: 15 of 93
            Pseudo Genes (multiple problems)  :: 23 of 93
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Salmonella enterica subsp. enterica serovar
                     Muenchen"
                     /mol_type="genomic DNA"
                     /strain="CVM N17S129"
                     /serovar="Muenchen"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:596"
                     /plasmid="pN17S129"
                     /geo_loc_name="USA:MD"
                     /collection_date="2017"
                     /collected_by="FDA"
     gene            1..840
                     /locus_tag="D7E29_023605"
     CDS             1..840
                     /locus_tag="D7E29_023605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAG67624.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /locus_tag="D7E29_023610"
     CDS             827..1678
                     /locus_tag="D7E29_023610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAG67625.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            1985..2800
                     /gene="sul2"
                     /locus_tag="D7E29_023615"
     ARG             1986..2797
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3246..3664
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             2862..3661
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3664..4500
                     /locus_tag="D7E29_023625"
     CDS             3664..4500
                     /locus_tag="D7E29_023625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAG67628.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            4806..5048
                     /locus_tag="D7E29_023630"
     CDS             4806..5048
                     /locus_tag="D7E29_023630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAG67629.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(5080..5628)
                     /gene="tetR(A)"
                     /locus_tag="D7E29_023635"
                     /pseudo=""
     CDS             complement(5080..5628)
                     /gene="tetR(A)"
                     /locus_tag="D7E29_023635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /name="tetR(A)"
     gene            complement(5693..5856)
                     /gene="tetR(A)"
                     /locus_tag="D7E29_023640"
                     /pseudo=""
     CDS             complement(5693..5856)
                     /gene="tetR(A)"
                     /locus_tag="D7E29_023640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /name="tetR(A)"
     ARG             6250..7160
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="71.46"
     ARG             5963..6306
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="99.12"
                     /coverage="26.89"
     gene            complement(7127..7315)
                     /locus_tag="D7E29_023650"
                     /pseudo=""
     CDS             complement(7127..7315)
                     /locus_tag="D7E29_023650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7913..8197)
                     /locus_tag="D7E29_023655"
     CDS             complement(7913..8197)
                     /locus_tag="D7E29_023655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAG67620.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            8396..10525
                     /locus_tag="D7E29_023660"
     CDS             8396..10525
                     /locus_tag="D7E29_023660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAG67621.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            10586..10798
                     /locus_tag="D7E29_023665"
     CDS             10586..10798
                     /locus_tag="D7E29_023665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAG67622.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            10800..11006
                     /locus_tag="D7E29_023670"
     CDS             10800..11006
                     /locus_tag="D7E29_023670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAG67623.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     ARG             3665..4497
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8152..8307
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          8397..10522
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 cccccactct attgtaaaca agacattttt atcttttata ttcaatggct tattttcctg
     1741 ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat attttgaaaa
     1801 attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt tgcttccaga
     1861 tgtatgctct tctgctcctg cagctaatgg atcaccgcaa acaggttact cgcctgggga
     1921 ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc
     1981 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
     2041 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
     2101 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
     2161 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
     2221 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
     2281 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
     2341 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
     2401 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
     2461 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     2521 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     2581 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     2641 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     2701 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     2761 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     2821 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     2881 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     2941 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     3001 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     3061 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     3121 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     3181 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc gcaggctgtc
     3241 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     3301 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     3361 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     3421 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     3481 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     3541 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     3601 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     3661 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     3721 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     3781 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     3841 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     3901 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     3961 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     4021 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     4081 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     4141 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     4201 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     4261 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     4321 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     4381 ctcgaccagg cgtacgctta tggatgcctt tccgcagctt ggaacgcgga tggagaagag
     4441 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     4501 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     4561 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gacccctgga agccccgtag
     4621 cgacgcggag aggggcgaga caagccaagg gcgcaggctc gatgcgcagc acgacatagc
     4681 cggttctcgc aaggacgaga atttccctgc ggtgcccctc aagtgtcaat gaaagtttcc
     4741 aacgcgagcc attcgcgaga gccttgagtc cacgctagat ctatctcatc tgcgcaaggc
     4801 agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac gaggccggcg
     4861 cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag aaggccgcca
     4921 gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa aagcccgtag
     4981 cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc tacatggctc
     5041 tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac cctttctcgg
     5101 tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag
     5161 tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa
     5221 cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc
     5281 ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac
     5341 ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc
     5401 catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag
     5461 cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc
     5521 tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag
     5581 cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccag cgccagtcgt
     5641 cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg gccagtgcgt
     5701 cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga accccaaccg
     5761 ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc
     5821 ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa
     5881 cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg
     5941 aggctggtcc ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact
     6001 gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc
     6061 gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg
     6121 ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg
     6181 ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg
     6241 cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg
     6301 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     6361 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     6421 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     6481 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     6541 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     6601 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     6661 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     6721 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     6781 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     6841 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     6901 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     6961 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     7021 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     7081 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agaaattctt
     7141 caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga
     7201 atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct
     7261 ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc
     7321 gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc
     7381 tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta
     7441 actgtcacgc ccccctgcaa taactgtcac gcccccctgc aataactgtc acgcccccct
     7501 gcaataactg tcacgccccc ctgcaataac tgtcacgaac ccctgcaata actgtcacgc
     7561 ccccaaacct gcaaacccag caggggcggg ggctggcggg gtgttggaaa aatccatcca
     7621 tgattatcta agaataatcc actaggcgcg gttatcagcg cccttgtggg gcgctgctgc
     7681 ccttgcccaa tatgcccggc cagaggccgg atagctggtc tattcgctgc gctaggctac
     7741 acaccgcccc accgctgcgc ggcaggggga aaggcgggca aagcccgcta aaccccacac
     7801 caaaccccgc agaaatacgc tggagcgctt ttagccgctt tagcggcctt tccccctacc
     7861 cgaagggtgg gggcgcgtgt gcagccccgc agggcctgtc tcggtcgatc attcagcccg
     7921 gctcatcctt ctggcgtggc ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt
     7981 acgcatccat tgccgccatg agccgatcct ccggccactc gctgctgttc accttggcca
     8041 aaatcatggc ccccaccagc accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt
     8101 cccttgcccg cacccgctga atttcggcat tgattcgcgc tcgttgttct tcgagcttgg
     8161 ccagccgatc cgccgccttg ttgctcccct taaccatctt gacaccccat tgttaatgtg
     8221 ctgtctcgta ggctatcatg gaggcacagc ggcggcaatc ccgaccctac tttgtagggg
     8281 agggcgcact taccggtttc tcttcgagaa actggcctaa cggccaccct tcgggcggtg
     8341 cgctctccga gggccattgc atggagccga aaagcaaaag caacagcgag gcagcatggc
     8401 gatttatcac cttacggcga aaaccggcag caggtcgggc ggccaatcgg ccagggccaa
     8461 ggccgactac atccagcgcg aaggcaagta tgcccgcgac atggatgaag tcttgcacgc
     8521 cgaatccggg cacatgccgg agttcgtcga gcggcccgcc gactactggg atgctgccga
     8581 cctgtatgaa cgcgccaatg ggcggctgtt caaggaggtc gaatttgccc tgccggtcga
     8641 gctgaccctc gaccagcaga aggcgctggc gtccgagttc gcccagcacc tgaccggtgc
     8701 cgagcgcctg ccgtatacgc tggccatcca tgccggtggc ggcgagaacc cgcactgcca
     8761 cctgatgatc tccgagcgga tcaatgacgg catcgagcgg cccgccgctc agtggttcaa
     8821 gcggtacaac ggcaagaccc cggagaaggg cggggcacag aagaccgaag cgctcaagcc
     8881 caaggcatgg cttgagcaga cccgcgaggc atgggccgac catgccaacc gggcattaga
     8941 gcgggctggc cacgacgccc gcattgacca cagaacactt gaggcgcagg gcatcgagcg
     9001 cctgcccggt gttcacctgg ggccgaacgt ggtggagatg gaaggccggg gcatccgcac
     9061 cgaccgggca gacgtggccc tgaacatcga caccgccaac gcccagatca tcgacttaca
     9121 ggaataccgg gaggcaatag accatgaacg caatcgacag agtgaagaaa tccagaggca
     9181 tcaacgagtt agcggagcag atcgaaccgc tggcccagag catggcgaca ctggccgacg
     9241 aagcccggca ggtcatgagc cagacccagc aggccagcga ggcgcaggcg gcggagtggc
     9301 tgaaagccca gcgccagaca ggggcggcat gggtggagct ggccaaagag ttgcgggagg
     9361 tagccgccga ggtgagcagc gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact
     9421 ggaagctatg gctaaccgtg atgctggctt ccatgatgcc tacggtggtg ctgctgatcg
     9481 catcgttgct cttgctcgac ctgacgccac tgacaaccga ggacggctcg atctggctgc
     9541 gcttggtggc ccgatgaaga acgacaggac tttgcaggcc ataggccgac agctcaaggc
     9601 catgggctgt gagcgcttcg atatcggcgt cagggacgcc accaccggcc agatgatgaa
     9661 ccgggaatgg tcagccgccg aagtgctcca gaacacgcca tggctcaagc ggatgaatgc
     9721 ccagggcaat gacgtgtata tcaggcccgc cgagcaggag cggcatggtc tggtgctggt
     9781 ggacgacctc agcgagtttg acctggatga catgaaagcc gagggccggg agcctgccct
     9841 ggtagtggaa accagcccga agaactatca ggcatgggtc aaggtggccg acgccgcagg
     9901 cggtgaactt cgggggcaga ttgcccggac gctggccagc gagtacgacg ccgacccggc
     9961 cagcgccgac agccgccact atggccgctt ggcgggcttc accaaccgca aggacaagca
    10021 caccacccgc gccggttatc agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc
    10081 caccgctggc ccggcgctgg tgcagcaggc tggccagcag atcgagcagg cccagcggca
    10141 gcaggagaag gcccgcaggc tggccagcct cgaactgccc gagcggcagc ttagccgcca
    10201 ccggcgcacg gcgctggacg agtaccgcag cgagatggcc gggctggtca agcgcttcgg
    10261 tgatgacctc agcaagtgcg actttatcgc cgcgcagaag ctggccagcc ggggccgcag
    10321 tgccgaggaa atcggcaagg ccatggccga ggccagccca gcgctggcag agcgcaagcc
    10381 cggccacgaa gcggattaca tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt
    10441 ccagcttgcg cgggccgagc tggcacgggc accggcaccc cgccagcgag gcatggacag
    10501 gggcgggcca gatttcagca tgtagtgctt gcgttggtac tcacgcctgt tatactatga
    10561 gtactcacgc acagaagggg gttttatgga atacgaaaaa agcgcttcag ggtcggtcta
    10621 cctgatcaaa agtgacaagg gctattggtt gcccggtggc tttggttata cgtcaaacaa
    10681 ggccgaggct ggccgctttt cagtcgctga tatggccagc cttaaccttg acggctgcac
    10741 cttgtccttg ttccgcgaag acaagccttt cggccccggc aagtttctcg gtgactgata
    10801 tgaaagacca aaaggacaag cagaccggcg acctgctggc cagccctgac gctgtacgcc
    10861 aagcgcgata tgccgagcgc atgaaggcca aagggatgcg tcagcgcaag ttctggctga
    10921 ccgacgacga atacgaggcg ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg
    10981 ggggtagtga ccccgccagc gcctaaccac caactgcctg caaaggaggc aatca
//
