LOCUS       CP082722               11038 bp    DNA     circular BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain CVM
            N17S288 isolate 17NY04GT13-S3 plasmid pN17S288, complete sequence.
ACCESSION   CP082722
VERSION     CP082722.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN20351457
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAR-2019
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 667x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 15:15:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,513
            CDSs (total)                      :: 4,393
            Genes (coding)                    :: 4,286
            CDSs (with protein)               :: 4,286
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 51 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 23 of 107
            Pseudo Genes (multiple problems)  :: 20 of 107
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="CVM N17S288"
                     /serovar="Reading"
                     /isolate="17NY04GT13-S3"
                     /isolation_source="Turkey Patties"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pN17S288"
                     /geo_loc_name="USA:NY"
                     /collection_date="2017-04"
                     /collected_by="NY"
     gene            1..2130
                     /locus_tag="KZZ27_022500"
     CDS             1..2130
                     /locus_tag="KZZ27_022500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAD50999.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            2191..2403
                     /locus_tag="KZZ27_022505"
     CDS             2191..2403
                     /locus_tag="KZZ27_022505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAD51000.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            2405..2611
                     /locus_tag="KZZ27_022510"
     CDS             2405..2611
                     /locus_tag="KZZ27_022510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAD51001.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            2641..3480
                     /locus_tag="KZZ27_022515"
     CDS             2641..3480
                     /locus_tag="KZZ27_022515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAD51002.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            3467..4318
                     /locus_tag="KZZ27_022520"
     CDS             3467..4318
                     /locus_tag="KZZ27_022520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAD51003.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            4626..5441
                     /gene="sul2"
                     /locus_tag="KZZ27_022525"
     ARG             4627..5438
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5502..5645
                     /locus_tag="KZZ27_022530"
                     /pseudo=""
     CDS             5502..5645
                     /locus_tag="KZZ27_022530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /name="aminoglycoside O-phosphotransferase APH(3'')-Ib"
     ARG             6014..6432
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             5648..6429
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="97.75"
     gene            6432..7268
                     /locus_tag="KZZ27_022540"
     CDS             6432..7268
                     /locus_tag="KZZ27_022540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAD50995.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            7574..7816
                     /locus_tag="KZZ27_022545"
     CDS             7574..7816
                     /locus_tag="KZZ27_022545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAD50996.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(7848..8498)
                     /gene="tetR(A)"
                     /locus_tag="KZZ27_022550"
     CDS             complement(7848..8498)
                     /gene="tetR(A)"
                     /locus_tag="KZZ27_022550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAD51005.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            8604..9803
                     /gene="tet(A)"
                     /locus_tag="KZZ27_022555"
     ARG             8605..9800
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9770..9958)
                     /locus_tag="KZZ27_022560"
                     /pseudo=""
     CDS             complement(9770..9958)
                     /locus_tag="KZZ27_022560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(10556..10840)
                     /locus_tag="KZZ27_022565"
     CDS             complement(10556..10840)
                     /locus_tag="KZZ27_022565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAD50998.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     ARG             6433..7265
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          10795..10950
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          2659..3480
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2..2127
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg
       61 gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg
      121 cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct
      181 gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg
      241 gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc
      301 ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac
      361 tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg
      421 ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc
      481 aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca
      541 ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc
      601 gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc
      661 cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac
      721 ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag
      781 aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc
      841 cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga
      901 gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg
      961 ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg
     1021 gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct
     1081 gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg
     1141 gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc
     1201 aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg
     1261 atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg
     1321 aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg
     1381 ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct
     1441 gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc
     1501 gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac
     1561 ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac
     1621 aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag
     1681 accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag
     1741 cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc
     1801 cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc
     1861 ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc
     1921 cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc
     1981 aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc
     2041 agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg
     2101 gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac
     2161 tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg
     2221 gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca
     2281 aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc
     2341 tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac
     2401 tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt
     2461 acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg
     2521 gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca
     2581 gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca
     2641 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
     2701 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
     2761 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
     2821 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
     2881 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
     2941 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
     3001 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
     3061 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
     3121 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
     3181 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
     3241 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
     3301 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
     3361 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
     3421 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
     3481 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
     3541 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
     3601 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     3661 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     3721 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     3781 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     3841 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     3901 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     3961 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     4021 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     4081 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     4141 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     4201 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     4261 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     4321 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     4381 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     4441 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     4501 atgtatgctc ttctgctcct gcagctaatg gatcaccgca aacaggttac tcgcctgggg
     4561 attccctttc gacccgagca tccgtatgag actcatgctc gattattatt attatagaag
     4621 cccccatgaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct
     4681 ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg
     4741 ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac gccgcgcctg
     4801 tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg
     4861 gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg
     4921 gtgtggccta tctcaatgat attcgcggtt ttccagacgc tgcgttctat ccgcaattgg
     4981 cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag gcagatcggc
     5041 gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac gcgcgcatcg
     5101 cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc ggcatggggt
     5161 tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc
     5221 ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc
     5281 tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag cttgccgccg
     5341 ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg
     5401 cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt
     5461 ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga actaatattt
     5521 tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct ggtgattttg
     5581 tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg
     5641 agctcttttt ttggtgaatc gcattctgac tggttgcctg tcagaggcgg agaatctggt
     5701 gattttgttt ttcgacgtgg tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc
     5761 cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct
     5821 tgccccgagg tcatcaactg gcaggaggaa caggagggtg catgcttggt gataacggca
     5881 attccgggag taccggcggc tgatctgtct ggagcggatt tgctcaaagc gtggccgtca
     5941 atggggcagc aacttggcgc tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc
     6001 aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc
     6061 gacttcttac cggacgagga caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa
     6121 cgagagctac cggtgcggct cgaccaagag cgcaccgata tggttgtttg ccatggtgat
     6181 ccctgcatgc cgaacttcat ggtggaccct aaaactcttc aatgcacggg tctgatcgac
     6241 cttgggcggc tcggaacagc agatcgctat gccgatttgg cactcatgat tgctaacgcc
     6301 gaagagaact gggcagcgcc agatgaagca gagcgcgcct tcgctgtcct attcaatgta
     6361 ttggggatcg aagcccccga ccgcgaacgc cttgccttct atctgcgatt ggaccctctg
     6421 acttggggtt gatgttcatg ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg
     6481 ttctcattgc ggacaccttt tccagcctcg tttggaaagt ttcattgcca gacgggactc
     6541 ctgcaatcgt caagggattg aaacctatag aagacattgc tgatgaactg cgcggggccg
     6601 actatctggt atggcgcaat gggaggggag cagtccggtt gctcggtcgt gagaacaatc
     6661 tgatgttgct cgaatatgcc ggggagcgaa tgctctctca catcgttgcc gagcacggcg
     6721 actaccaggc gaccgaaatt gcagcggaac taatggcgaa gctgtatgcc gcatctgagg
     6781 aacccctgcc ttctgccctt ctcccgatcc gggatcgctt tgcagctttg tttcagcggg
     6841 cgcgcgatga tcaaaacgca ggttgtcaaa ctgactacgt ccacgcggcg attatagccg
     6901 atcaaatgat gagcaatgcc tcggaactgc gtgggctaca tggcgatctg catcatgaaa
     6961 acatcatgtt ctccagtcgc ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag
     7021 tgggctttgg cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg
     7081 atcctagacg cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc
     7141 gtcgcctgct cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg
     7201 gagaagagga gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt
     7261 catactagat atcaagcgac ttctcctatc ccctgggaac acatcaatct caccggagaa
     7321 tatcgctggc caaagcctta gcgtaggatt ccgccccttc ccgcaaacga cccctggaag
     7381 ccccgtagcg acgcggagag gggcgagaca agccaagggc gcaggctcga tgcgcagcac
     7441 gacatagccg gttctcgcaa ggacgagaat ttccctgcgg tgcccctcaa gtgtcaatga
     7501 aagtttccaa cgcgagccat tcgcgagagc cttgagtcca cgctagatct atctcatctg
     7561 cgcaaggcag aacgtgaaga cggccgccct ggacctcgcc cgcgagcgcc aggcgcacga
     7621 ggccggcgcg cggacccgcg ccacggccca cgagcggacg ccgcagcagg agcgccagaa
     7681 ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa
     7741 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
     7801 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
     7861 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
     7921 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
     7981 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
     8041 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
     8101 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
     8161 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
     8221 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
     8281 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
     8341 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
     8401 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
     8461 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
     8521 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
     8581 ggaggctggt ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca
     8641 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
     8701 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
     8761 cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
     8821 ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg
     8881 cgcctttcct ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg
     8941 gggcggtagc cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact
     9001 tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc
     9061 tgatgggcgg tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca
     9121 atttcctgac gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac
     9181 gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg
     9241 ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt
     9301 gggtcatttt cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg
     9361 ccgcatttgg cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc
     9421 ggctcggcga aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc
     9481 tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg
     9541 gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc
     9601 aggggcagct gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc
     9661 tcctcttcac ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg
     9721 caggcgctgc cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagaaatt
     9781 cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag
     9841 cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc
     9901 tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt
     9961 tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
    10021 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta
    10081 ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc
    10141 cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca ataactgtca
    10201 cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg aaaaatccat
    10261 ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt ggggcgctgc
    10321 tgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc tgcgctaggc
    10381 tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca
    10441 caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc ctttccccct
    10501 acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc
    10561 ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa
    10621 ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg
    10681 ccaaaatcat ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct
    10741 gttcccttgc ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct
    10801 tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat
    10861 gtgctgtctc gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag
    10921 gggagggcgc acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg
    10981 gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagc
//
