LOCUS       CP082703               10510 bp    DNA     circular BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain CVM
            N17S546 plasmid pN17S546-2, complete sequence.
ACCESSION   CP082703
VERSION     CP082703.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN10132878
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10510)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAR-2019
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 697x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/01/2021 17:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,523
            CDSs (total)                      :: 4,403
            Genes (coding)                    :: 4,297
            CDSs (with protein)               :: 4,297
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 46 of 106
            Pseudo Genes (incomplete)         :: 59 of 106
            Pseudo Genes (internal stop)      :: 24 of 106
            Pseudo Genes (multiple problems)  :: 20 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="CVM N17S546"
                     /serovar="Reading"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pN17S546-2"
                     /geo_loc_name="USA:IA"
                     /collection_date="2017"
                     /collected_by="FDA"
     gene            complement(join(8589..10510,1..1083))
                     /locus_tag="D7F29_022565"
                     /pseudo=""
     CDS             complement(join(8589..10510,1..1083))
                     /locus_tag="D7F29_022565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            1247..1804
                     /locus_tag="D7F29_022570"
     CDS             1247..1804
                     /locus_tag="D7F29_022570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAH08471.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            1987..2847
                     /locus_tag="D7F29_022575"
     CDS             1987..2847
                     /locus_tag="D7F29_022575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UAH08472.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(3518..3730)
                     /locus_tag="D7F29_022580"
     CDS             complement(3518..3730)
                     /locus_tag="D7F29_022580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAH08473.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3734..4219)
                     /locus_tag="D7F29_022585"
     CDS             complement(3734..4219)
                     /locus_tag="D7F29_022585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAH08474.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(4194..4934)
                     /locus_tag="D7F29_022590"
                     /pseudo=""
     CDS             complement(4194..4934)
                     /locus_tag="D7F29_022590"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4897..5217)
                     /locus_tag="D7F29_022595"
     CDS             complement(4897..5217)
                     /locus_tag="D7F29_022595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAH08475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5252..5344)
                     /locus_tag="D7F29_022600"
                     /pseudo=""
     CDS             complement(5252..5344)
                     /locus_tag="D7F29_022600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            6532..6993
                     /locus_tag="D7F29_022605"
     CDS             6532..6993
                     /locus_tag="D7F29_022605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH08476.1"
                     /translation="MSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDL
                     NGLVFGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHI
                     NDNKEKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            complement(7022..7585)
                     /locus_tag="D7F29_022610"
     CDS             complement(7022..7585)
                     /locus_tag="D7F29_022610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH08469.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(7843..8253)
                     /locus_tag="D7F29_022615"
     CDS             complement(7843..8253)
                     /locus_tag="D7F29_022615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH08470.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     ARG             1988..2844
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5453..5632)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          5578..6091
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.217"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3403..4907)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.426"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgccttggg atgtagctga acacctcagc acgaatcgat tcgtccggtg tttcttcttt
       61 cagcaggtac gaacatgcgc tggcgagcgc caatgcagat ttatccagat ccttcagcga
      121 gcggagccgt tttttctgcc caatctttct ggcgtcacgg atgataacgg ccagcatggc
      181 gtccagaacg tccaatgcat catccagcgc cagcgtttcc catgcaagga caaaggcaac
      241 cagaaccgcc atccttttct gcggtgacat cctggcaata ttgaacaccg aagtcatacc
      301 agcataacgt gcgagatttt tcaggcgcac agccgggagt gtactcaggt tttcagcatg
      361 caggccaaaa tcgttcagag ttttccagcg ttcaattgct tcattaaacg ccggaccact
      421 gatggtcaca gggccctttt tcagtgattc cagtaaagac aggcggctgc aatcagttgg
      481 ccccagcagc atctccagct gtgaacgctg ttcggctgac ggtatcagtg ccagtttgtt
      541 ccacaggcgc aacgtcgcct tttcccttac ctctgaaatc aaccgggtca gcgtagtggc
      601 tccggggaga ataatacgat gttgcataag ccaccctgtc gccagatcga aaagcaggcc
      661 aggacgttcg ttgcttatcc agctccgggt atataaaaga cgggtaaggc gaaatgtcca
      721 gggccaggca aattcacgat actgatagtg ctgacgtatc agcgctgcat gctcacggcg
      781 ggtattttcc ctctgaccgt attctgcaag aacggtgata tcacgaatcc cgagctgtct
      841 ggcggtaaaa tgccggacgc cggaaggaat atgattcata tcggtgagga aggtgcccag
      901 aaaacggaca catccaattt gcagggcaat gcccagacgg ttgtgatcac ctctgctttt
      961 tccgataaat tccttgtctg cttcatcaag gtgaaaatat cgtgccagct gaagctcatc
     1021 cggttcaccg gtgaatctgc catagctttc agtctgctca gtggtcagaa agtcaacggg
     1081 catatcggcc tccctgcctg acgggcattt agtaacattt ttgcaaccgt acgaaatgtt
     1141 ataaattatc ggacatcgta aaactgttac attaatatgt ctattaaatc gtaaatttgt
     1201 aataatagac atgagttgtc cgatattcga tttaaggtac atttttatgc gactttttgg
     1261 ttacgctcgg gtctcaacca gtcagcagtc tcttgatctt caggtcagag cactcaaaga
     1321 cgcaggtgtg aaagcaaacc gtatatttac cgataaggca tccggcagtt caacagaccg
     1381 ggaagggctg gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa
     1441 gctcgaccgt cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc
     1501 tcagggcgtg gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca
     1561 aatggtggtc accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg
     1621 cacgaatgag ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac
     1681 cgtggacagg aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc
     1741 tcatcagctc agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc
     1801 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta
     2221 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2521 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2881 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2941 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtatag
     3001 gttgccgatg agatgaattt aacataagag ttcttgcccg caccgcttcg ggctggttcg
     3061 gcgcaaatac ggtgcctgtc gggtgaaacc cctagcaaag cacccggtaa accgccccgt
     3121 ttacgccaga taaatttcac ttggctgcat ggttatgcag ccagatgaat gacagcagtt
     3181 ttcgcgtata aatacggtgt aaagcactaa aaaggccact ttcccccgaa tcgctcccac
     3241 agatccattg ccagtttgac gcattgcagt atcagactga gcgcttttag tatcttcata
     3301 aatccccctc tcttgagccg tgatcttttc atcacgaccg tatttagcgg gaaatttatg
     3361 agcccttctt cttggggttt gtcactggat gcgcgcagtc agcccagcgt cggaccgtta
     3421 tagctccgct gacggaaacg gggttctgcc agctcttttt catgctgtcg ctgctcgtat
     3481 gcccgttcgg cggccaccgc catttcatgc cgctgcacta ctggctcaaa cgctgtaact
     3541 gcctgctcaa gttcgccacc tgctgactca agcgcgtgac gcgctcgttc agcgccgcgt
     3601 tctcccgttg cataagacca gacatcttct gccattccgc gaaggcgctc tcccactcgt
     3661 ccagcctttg cgagtagtcc tgatgtagct gctctaatgc ggtcagcaac tgtttttcca
     3721 gttccgtcat gggctatttc ctctccagta ccatccagtt cccttcctcc ccgtaagctc
     3781 ccgctttcgg gttcactttg acgcaggaca gtttctgttc cccacagagc gacagctgca
     3841 cgccgtggtt cttctccgac agcatggcct gcgtccgctc ctgcgcccgg atagtctggt
     3901 aattcccggt gatctgctgc ccctgccacc acagaatgct cccgctcgtg gcgatcagca
     3961 gtactgagac cagggtgatg gtcagccacg tccggccaac catacgcagc acgctcagcc
     4021 ggttcgactg catcgccgcg gtcataccct gctcgtgggc gctgatggcg tcgctgattc
     4081 tcttcgcgct tgcgctcagt tccgctttta cagacttctc gtgctcgctg aatgcggctt
     4141 tcagcatctc gcaggtattc tgctgctgcg ctttcgattg cttttccaag tccttcgcca
     4201 gcgctaaaag actgctcata aatggctccc ttcagtcgga tgtttcgccc cccgtccggg
     4261 tcggcaatgc tgatactgtt ctttgtggtg cggaccacct caaaacctgc cgcagtgagc
     4321 gaccctgtga cgtcctcgcg cgttttgagc gccccggatg aggctaaggc cagcaaaccc
     4381 cgtgtaatcg cctgagcggc ttccagcttc gtttccggca gtgcggacgg cgtgaccagt
     4441 aaccgccggt tcttcggggc gtttggatca tgcagcccca gtctgccgtt caccacggtc
     4501 tgccaggcat cgatgcgcgg ccggtcggcg cgatcgtaat acggctgcag gcgtttcccg
     4561 gtcagcagct ctgtgttggg gatcaggaaa ttcagctcca gccgcccctt gtcggtgtgc
     4621 tccacccaca gaatgctgta ctggtcttta tcgagcccgg gcatcagaac ccgctcaaag
     4681 ctggccatga tctgctcccg ctgcccgggc ggcagctcag cctccgcgaa cgacagtacg
     4741 ccggaggtgt actttttcgc atacggcgag gcatcgataa gctcccggac ttcctccggc
     4801 tttccctgca gcacggtcgc cccctcgcgc tgccggtctt ttcccagcag gtaatccacc
     4861 ggaccggcac caccaccgcg cccccgggga tggaatttaa cgatcatcat tccccccttt
     4921 ttccagcacc gcatgccgca gccgctccag tcccgcgtcg attgccatca gggcggccac
     4981 gatctgcacc cggtcatgac cggcaccacc ggtattcact ttccgggcga tctggttcag
     5041 gttattaccc ataccggcaa gctggcgcag cagcgccggc gatatcgacg gcagtctgcc
     5101 ggcacgcgcc ggcttctcat cgaggcaggt ctgacgcatc cacgccgcca gctgcttgcc
     5161 gtcgcaccgc tcgagcagcc gccggtgttc gtcttccgtc acccacatcg tgagcatctt
     5221 gttgcgtttg tctgccagag tagcctcctg agcaggtctg acgcatccac gccgccagct
     5281 gcttgccgtc gcaccgctcg agcagccgcc ggtgttcgtc ttccgtcacc cacatcgtga
     5341 gcatcttgtt gcgtttgtct gccagagtag cctcctgata acaccgggac gggcgggaaa
     5401 tgtttcccgg tcggcacggt gttggacaag ccgcaggcgc gtcagtggct tttagcgggt
     5461 ttcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggcttaatat
     5521 gttacggcgg agtaacactt tataaaagtg cttcatgtgg caggggaaaa tgcagctgca
     5581 gggcgtacgg gagaacatgg aggttgcggt gatattacgc ctcctcgctc actcggtcgc
     5641 ttccgctcct gccgttcggc tgcggcgggc gttacctgca cacggataag agcaaaggca
     5701 tacggcatga caaacagggc aagaaaagga tgcggcgctg ccgtttttcc ataggctccg
     5761 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     5821 actataaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc ctgttcctgc
     5881 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttatctcatt ccacgcctga
     5941 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
     6001 agtccgaccg ctgcgccttt tccggtaact atcgacttga gtccaacccg gaaagacacg
     6061 actaatcgcc attggcaacg gccactggta aacatgatgt gcgagagatg tattgagagt
     6121 tcttgaagtg gtggccttac tgaggctaca ctggaaggac ggatttggtg acacgtctcg
     6181 cacaagactg ttaccacggt tagataatct gccaggatta aataaccttc gaaaaaccac
     6241 ctgccagggt ggttttttcg tttcaggaga agattacgct ggcaaagtaa gtccagtcaa
     6301 gaacagctac gaaattgact tcacatataa tttttgatat aagtaaatct agaagtggcg
     6361 gtaggaatga gccccacgtt aaacaaacaa gcttccaaag aaagatgtgc ggtagagttc
     6421 gccacacgta aaaaatccaa gactcaacga aagaaaagca aagccctctc ttacagaggg
     6481 ctttgctttt tttgtacatt ggttacagga aaatgccaat ggacatcaaa aatgagcaaa
     6541 caggattaca tacagttaag aacccttaca gaccagtttt atgctgataa caaaggccta
     6601 caagaggctc tcgacggcag taatgatgga aaagtaagag gctacggaat cgtagttatt
     6661 gatctcaacg gattggtgtt cgggataccc ttacgaagcc atttaaacca taaatttgga
     6721 ttcgtttcgg agcgatcaga aggtgttaag aaaggactgg attacaccaa agcgttgcta
     6781 attaagaaag aagagtatgt atcgcgagcc tataagatac caactcctga atttacccat
     6841 atcaatgata acaaagaaaa aatacaagaa gatttcaaca aattcgtaaa cagatacata
     6901 gaagcgaacg tcaagaaaga tgaaaacata ctcagaaact atcgttactc tacgctgaaa
     6961 aactatcaca aagaattagg tttagaagac taaaaaggcg gtctaacgac cgcccttgcc
     7021 tttacgccgc gctcaattca tccagcatat gggtgaactt atcttctatc tccttccaca
     7081 tctcgtcaga aagaggttta tctttgtagg cgtgatgtag aaggcttaag acggcttgag
     7141 aaacactacc ttttttctcg ataagcttgc cgagattttt cccttctttt tcgagagaac
     7201 gaaagaagag gctcctttcc tgattagcaa acacaagctc aggatcagcg agaagttgct
     7261 cgatgtgttc aggctctatg aaatagacag tttgtccagt catccttcct gtagttacag
     7321 atttaacaga tacatttaga tgacgaatag ggaaaaaata ttttttctct aacatctcga
     7381 taatcgccgg taagcgagtg acctcaaagt cggctttgac actgttacca gaactggtga
     7441 ggtgatggcc ttcgatcagt gatgacaaga caacaacttg ggaatcctgc atcgtgttga
     7501 agagctcgat caccttcaaa tgagacgaga atttagttgc caggttagcg cgggcaactg
     7561 aaggtagtat tgataaagcg tgcatatgta tagcctcata tgtttttaaa aacataaact
     7621 taagagacca aagccaatag catcgcacct atcagaatag aaaatgtcgt gttcacatcc
     7681 ctcaaaagaa acgatttttt ctttattaga atgcaagttt atgaacataa tgccgcccat
     7741 aacactccac ccttccaaag gtagtgatgt gttgacaaga aggcggagtt agtcgattgt
     7801 ttggcgacgc tcactaactc tgcctttcct ttttggtcct tcctattcat catccagcct
     7861 gaagacgatt acaccttcac ttagctgagt gaatgaatca tcggaaagta gcttcttgtt
     7921 gtcctcttca tcctcagaaa ggcgaggaaa acctggtcgc aaggtcaatc taacagcaca
     7981 cgatgagtaa ccgccgtgct tgtttttttg cccagacaca ccaaagccac cttcactaat
     8041 tttcttaatc atccactggt tttcttcttt gtcgtaaaga atgtcgacac gatccccaat
     8101 ggtaatacca gtctcgtcca tagcgctctt gctcaagcgg aatcccagct cgacagcgcc
     8161 cgtgcttttt ttcgtttgcg tggccgtaac acactgcgac tggattatcc ttccagtctt
     8221 cgctgccaag ttcgatagag agataaaagc cataatacaa tccgttttag acatcgatca
     8281 gttcttatac ggaagtgtac ccagggagcg aggtggcttt caagatcttt tgtattgatt
     8341 tttctagagc cgcaaaaaac ggcgataaga aaccttaaat ataaaaacaa ttgctcgtat
     8401 tgaacctcgt caagatacac atccagatcg atctttatgt aagtaaaggg tatttttcta
     8461 tggtgattta aagtcgagca acgtcagcgc atccaaaaaa tcaccaagta ggtgcaagaa
     8521 agccgcttat agaaactgat ttttatgcct gtataggggt ctgacgctca gtggaacgaa
     8581 aactcacgtt aagcaacgtt ttctgcctct gacgcctctt ttaatggtct cagatgtcct
     8641 ttggtcacca gttctgccag cgtgaaggaa taatggccga gcatattgat atgtccgtgg
     8701 caaagcgggg agaggcgtgc gatatcttca tcattcagtg tttcaccctg cgcccggaga
     8761 tgatccaggg ctgcctgcat ataaatagtg ttccataaca cgacggcgtt agtgaccagc
     8821 cccagtgcgc ccagttgatc ttcctgaccg tcggtatatc gttttcttat ctcacctttt
     8881 tgaccgtgac agatggctct ggcaacggca tggcgacttt ctccccgatt aagctgggtc
     8941 agaatgcgcc ggcggtaatc ttcatcatca atataattaa gcagatacag cgttttgttg
     9001 atgcgcccca cttcaatgat tgcctgagtc agtccggaag gacgttcact tttcagcaat
     9061 gaacggacca gcactgaagc ctgtactttg cccagcttca gggagccagc ggtccggatc
     9121 atttcgtccc actgaaggac tatttttcgg ggatctgatt gccctctggc aatatcattc
     9181 agcacgccat agtcggcatc atggtccatt cgccagaaaa ccgaagcacc ggcatcagcc
     9241 aggcgtggag aaaactggta tcccagcagc cagaaaaggc caaagacaag ttcgctggca
     9301 cctgctgtat cggtcataat ttcggttgga ttcagcccgg tctcctgttc cagaagacct
     9361 tccagcacaa agatagagtc cctcagcgtc cccggtataa cgatgccatg aaagccggaa
     9421 tactgatcgg acacaaagtt gtaccaggtg atccctctgt tattaccaaa gtatttgcgg
     9481 ttcggtccgg cattgattgt tctgactggc gtaacaaagc gcattccatc tgcagatgcc
     9541 acttctcctc caccccatat ctgtgccagt ggcagcgttg cctgaaaatc aaccagtctg
     9601 gcattagcgc tggtgatagt ttcagcccgc agatagttcg cttttgtcca gttcagccgg
     9661 tgtcgggtca gtgcaggaac atttgatctg atcagtggtt ccagaccgat attgcaggct
     9721 tcagccatca gcacggcgct gatgctgacg ggcagatcat caactctggc actggcttca
     9781 ctagcatgga aaaactcatc agcaaatccg gtatgggcgt taatttcgag cagcaactcc
     9841 gttaaatcca ccggagggag tagatcactg atcattttgc tcagtcgttt cagactgtcc
     9901 ggctcatcaa gactggcgag gggagaaatt gtcaaccggg gcttcgggcc agaaacatcg
     9961 agttcgacag cctcattttc gcaaagacgt gcagcaacct gtctgtaacg actatcaagc
    10021 tgatgaccca gagattttat tgcttcctgc gggtctgtcg ggtgccccaa agaacgataa
    10081 accttaatcc ggtttgcctg ccagtcagca ccctgtagta atcttgcacg aggatctccc
    10141 accggttact gccggtaacg tagacatccc tccgcctcag actatcctgc agtttactga
    10201 gaaagcagag cgtgtatccc ctgcgggtga tatgtttttc cttgttaatc accagccgtt
    10261 tccatgaccg actgataatt tccgttggtg cgtcgtcaaa aaactgccgc cgtgagctga
    10321 actcccggct gaggtagtca caggcattca gagtggtaac cccggcaggt gcggatgaaa
    10381 atttaacggt attcagcaga tggggcagga aacgacgaac gcgcccgtac tgctccacca
    10441 tttcttcatg aaaattatcg tctgagggcc gggcaatttc acggacaagc gtgatgattt
    10501 cagccagctt
//
