LOCUS       CP082702               11036 bp    DNA     circular BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain CVM
            N17S546 plasmid pN17S546-1, complete sequence.
ACCESSION   CP082702
VERSION     CP082702.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN10132878
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 11036)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAR-2019
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 697x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/01/2021 17:00:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,523
            CDSs (total)                      :: 4,403
            Genes (coding)                    :: 4,297
            CDSs (with protein)               :: 4,297
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 46 of 106
            Pseudo Genes (incomplete)         :: 59 of 106
            Pseudo Genes (internal stop)      :: 24 of 106
            Pseudo Genes (multiple problems)  :: 20 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="CVM N17S546"
                     /serovar="Reading"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pN17S546-1"
                     /geo_loc_name="USA:IA"
                     /collection_date="2017"
                     /collected_by="FDA"
     gene            161..445
                     /locus_tag="D7F29_022495"
     CDS             161..445
                     /locus_tag="D7F29_022495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UAH08463.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            1042..1230
                     /locus_tag="D7F29_022500"
                     /pseudo=""
     CDS             1042..1230
                     /locus_tag="D7F29_022500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1197..2396)
                     /gene="tet(A)"
                     /locus_tag="D7F29_022505"
     ARG             complement(1201..2396)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2502..3152
                     /gene="tetR(A)"
                     /locus_tag="D7F29_022510"
     CDS             2502..3152
                     /gene="tetR(A)"
                     /locus_tag="D7F29_022510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UAH08468.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(3184..3426)
                     /locus_tag="D7F29_022515"
     CDS             complement(3184..3426)
                     /locus_tag="D7F29_022515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UAH08465.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(3732..4568)
                     /locus_tag="D7F29_022520"
     CDS             complement(3732..4568)
                     /locus_tag="D7F29_022520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UAH08466.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(4569..4987)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(4572..5371)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5432..5632)
                     /locus_tag="D7F29_022530"
                     /pseudo=""
     CDS             complement(5432..5632)
                     /locus_tag="D7F29_022530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            complement(5634..6374)
                     /gene="folP"
                     /locus_tag="D7F29_022535"
                     /pseudo=""
     CDS             complement(5634..6374)
                     /gene="folP"
                     /locus_tag="D7F29_022535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="folP"
     gene            complement(6681..7532)
                     /locus_tag="D7F29_022540"
     CDS             complement(6681..7532)
                     /locus_tag="D7F29_022540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UAH08458.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(7519..8358)
                     /locus_tag="D7F29_022545"
     CDS             complement(7519..8358)
                     /locus_tag="D7F29_022545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UAH08459.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(8388..8594)
                     /locus_tag="D7F29_022550"
     CDS             complement(8388..8594)
                     /locus_tag="D7F29_022550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH08460.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(8596..8808)
                     /locus_tag="D7F29_022555"
     CDS             complement(8596..8808)
                     /locus_tag="D7F29_022555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH08461.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(8869..10998)
                     /locus_tag="D7F29_022560"
     CDS             complement(8869..10998)
                     /locus_tag="D7F29_022560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UAH08462.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     ARG             complement(5635..6374)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="91.14"
     ARG             complement(3736..4568)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5518..6374)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="105.17"
     Typing          complement(52..207)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(7520..8341)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(8873..10998)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggccctcg gagagcgcac cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa
       61 accggtaagt gcgccctccc ctacaaagta gggtcgggat tgccgccgct gtgcctccat
      121 gatagcctac gagacagcac attaacaatg gggtgtcaag atggttaagg ggagcaacaa
      181 ggcggcggat cggctggcca agctcgaaga acaacgagcg cgaatcaatg ccgaaattca
      241 gcgggtgcgg gcaagggaac agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct
      301 ggtgggggcc atgattttgg ccaaggtgaa cagcagcgag tggccggagg atcggctcat
      361 ggcggcaatg gatgcgtacc ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc
      421 acgccagaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca
      481 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctccagcg
      541 tatttctgcg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc
      601 agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg
      661 ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat
      721 tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg
      781 gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg
      841 gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga cagttattgc
      901 aggggttcgt gacagttagt acgggagtga cgggcactgg ctggcaatgt ctagcaacgg
      961 caggcatttc ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac
     1021 agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg
     1081 gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac
     1141 gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat
     1201 gtgcaacggg aatttgaaga atttctccaa agcccgcgac gcagcgccgg caggcagagc
     1261 aagtagaggg cagcgcctgc aatccatgcc cacccgttcc acgttgttat agaagccgca
     1321 tagatcgccg tgaagaggag gggtccgacg atcgaggtca ggctggtgag cgccgccagt
     1381 gagccttgca gctgcccctg acgttcctca tccacctgcc tggacaacat tgcttgcagc
     1441 gccggcattc cgatgccacc cgaagcaagc aggaccatga tcgggaacgc catccatccc
     1501 cgtgtcgcga aggcaagcag gatgtagcct gtgccgtcgg caatcattcc gagcatgagt
     1561 gcccgccttt cgccgagccg ggcggctaca gggccggtga tcattgcctg ggcgagtgaa
     1621 tgcagaatgc caaatgcggc aagcgaaatg ccgatcgtgg tcgcgtccca gtgaaagcga
     1681 tcctcgccga aaatgaccca aagcgcggcc ggcacctgtc cgacaagttg catgatgaag
     1741 aagaccgcca tcagggcggc gacgacggtc atgccccggg cccaccggaa cgaagcgagc
     1801 gggttgagag cctcccggcg taacggccgg cgttcgcctt tgtgcgactc cggcaaaagg
     1861 aaacagcccg tcaggaaatt gaggccgttc aaggctgccg cggcgaagaa cggagcgtgg
     1921 ggggagaaac cgcccatcag cccaccgagc acaggtcccg cgaccatccc gaacccgaaa
     1981 caggcgctca tgaagccgaa gtgccgcgcg cgctcatcgc catcagtgat atcggcaata
     2041 taagcgccgg ctaccgcccc agtcgccccg gtgatgccgg ccacgatccg cccgatatag
     2101 agaacccaaa ggaaaggcgc cgtcgccatg atggcgtagt cgacagcagc gccggccagc
     2161 gagacgagca agaccggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg
     2221 caggcaaatt gcatcaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg
     2281 ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg
     2341 acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtctgttggg tttcacgtct
     2401 ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag
     2461 ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga
     2521 atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga
     2581 cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga
     2641 acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt
     2701 cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca
     2761 ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg
     2821 caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg
     2881 ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg
     2941 aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc
     3001 tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt
     3061 tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga
     3121 acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac
     3181 tcactacagc agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc
     3241 gtagcagccc gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg
     3301 ctcggcctct ctggcggcct tctggcgctc ctgctgcggc gtccgctcgt gggccgtggc
     3361 gcgggtccgc gcgccggcct cgtgcgcctg gcgctcgcgg gcgaggtcca gggcggccgt
     3421 cttcacgttc tgccttgcgc agatgagata gatctagcgt ggactcaagg ctctcgcgaa
     3481 tggctcgcgt tggaaacttt cattgacact tgaggggcac cgcagggaaa ttctcgtcct
     3541 tgcgagaacc ggctatgtcg tgctgcgcat cgagcctgcg cccttggctt gtctcgcccc
     3601 tctccgcgtc gctacggggc ttccaggggt cgtttgcggg aaggggcgga atcctacgct
     3661 aaggctttgg ccagcgatat tctccggtga gattgatgtg ttcccagggg ataggagaag
     3721 tcgcttgata tctagtatga cgtctgtcgc acctgcttga tcgcggccgc gatagctaga
     3781 tcgcgttgct cctcttctcc atccgcgttc caagctgcgg aaaggcaccc ataagcgtac
     3841 gcctggtcga gcaggcgacg cggatcgacg tccagcgcac gagagaatgc gtccgccatc
     3901 tgtgcaatgc gtctaggatc gagacaaagg tcgtctctgt cagccggatc gtagaacata
     3961 ttggcggcgc caaagcccac ttcaccgacc agaccgacgg gatctatcac cagccagccg
     4021 cgactggaga acatgatgtt ttcatgatgc agatcgccat gtagcccacg cagttccgag
     4081 gcattgctca tcatttgatc ggctataatc gccgcgtgga cgtagtcagt ttgacaacct
     4141 gcgttttgat catcgcgcgc ccgctgaaac aaagctgcaa agcgatcccg gatcgggaga
     4201 agggcagaag gcaggggttc ctcagatgcg gcatacagct tcgccattag ttccgctgca
     4261 atttcggtcg cctggtagtc gccgtgctcg gcaacgatgt gagagagcat tcgctccccg
     4321 gcatattcga gcaacatcag attgttctca cgaccgagca accggactgc tcccctccca
     4381 ttgcgccata ccagatagtc ggccccgcgc agttcatcag caatgtcttc tataggtttc
     4441 aatcccttga cgattgcagg agtcccgtct ggcaatgaaa ctttccaaac gaggctggaa
     4501 aaggtgtccg caatgagaac aggttgcgaa acgtgccaat gagcaggaaa aacaggcggc
     4561 atgaacatca accccaagtc agagggtcca atcgcagata gaaggcaagg cgttcgcggt
     4621 cgggggcttc gatccccaat acattgaata ggacagcgaa ggcgcgctct gcttcatctg
     4681 gcgctgccca gttctcttcg gcgttagcaa tcatgagtgc caaatcggca tagcgatctg
     4741 ctgttccgag ccgcccaagg tcgatcagac ccgtgcattg aagagtttta gggtccacca
     4801 tgaagttcgg catgcaggga tcaccatggc aaacaaccat atcggtgcgc tcttggtcga
     4861 gccgcaccgg tagctctcgt tcgacacgag ccaaaagatc gagctgcggc gtactcttgt
     4921 cctcgtccgg taagaagtcg ggattgacgg cattgcggga caccacatca acggcgcgtc
     4981 cgaacattcg cgacagcctg cgctcaaacg gacattgatc aaccgatagg ctgtgaacag
     5041 cgccaagttg ctgccccatt gacggccacg ctttgagcaa atccgctcca gacagatcag
     5101 ccgccggtac tcccggaatt gccgttatca ccaagcatgc accctcctgt tcctcctgcc
     5161 agttgatgac ctcggggcaa gccacacctc gacctttgag ccaaatgagg cggtcacgct
     5221 ctccagcgag ctcaccgcgg cgggaagcag gtgcgatttt cgcgaaggca tgcccgtcac
     5281 cacgtcgaaa aacaaaatca ccagattctc cgcctctgac aggcaaccag tcagaatgcg
     5341 attcaccaaa aaaaatatta gttcgattca atggaggttc cttcagtttt ctgatgaagc
     5401 gcgaatatag agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt tctttcagcg
     5461 ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg atgaagtcag
     5521 ctccacctgc ggcggcggca agctctgcag cgagtgtcgc ggccccgaca tcccccggac
     5581 cacggcctgt gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc accgcggatg
     5641 aagtcagctc cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc cccgacatcc
     5701 cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga cagaagcacc
     5761 ggcaaatcga agcgcagccg caattcatcg aaccgcgcca gcaccgagag cgaggtttcg
     5821 ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt gcgtttgata
     5881 ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg atccatgatg
     5941 tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg cataacgacg
     6001 agtttggcag atgatttcgc caattgcgga tagaacgcag cgtctggaaa accgcgaata
     6061 tcattgagat aggccacacc acgcgacaag gcataggctt gcgtcgcggg ttgataactg
     6121 tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc gatacgcgcg
     6181 atttctgtgt cggacgaaac aggcgcggcg tcgggattgc tggatgccgg accgaggtcg
     6241 atcacatctg ccccctcggc catcagctta cgcgcctgcg caatggctgc gtctggcgcc
     6301 agataccggc ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc gaaaatgatg
     6361 agcgatttat tcatgggggc ttctataata ataataatcg agcatgagtc tcatacggat
     6421 gctcgggtcg aaagggaatc cccaggcgag taacctgttt gcggtgatcc attagctgca
     6481 ggagcagaag agcatacatc tggaagcaaa gccaggaaag cggcctatgg agctgtgcgg
     6541 cagcgctcag taggcaattt ttcaaaatat tgttaagcct tttctgagca tggtattttt
     6601 catggtatta ccaattagca ggaaaataag ccattgaata taaaagataa aaatgtcttg
     6661 tttacaatag agtggggggg tcagcctgcc gccttgggcc gggtgatgtc gtacttgccc
     6721 gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc ctcgcgcacc
     6781 cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac atagccgcac
     6841 aaggtatcta tggaagcctt gccggttttg ccggggtcga tccagccaca cagccgctgg
     6901 tgcagcaggc gggcggtttc gctgtccagc gcccgcacct cgtccatgct gatgcgcaca
     6961 tgctggccgc cacccatgac ggcctgcgcg atcaaggggt tcagggccac gtacaggcgc
     7021 ccgtccgcct cgtcgctggc gtactccgac agcagccgaa acccctgccg cttgcggcca
     7081 ttctgggcga tgatggatac cttccaaagg cgctcgatgc agtcctgtat gtgcttgagc
     7141 gccccaccac tatcgacctc tgccccgatt tcctttgcca gcgcccgata gctacctttg
     7201 accaccatgg catcagcggt gacggcctcc cacttgggtt ccaggaacag ccggagctgc
     7261 cgtccgcctt cggtcttggg ttccgggcca agcactaggc cattaggccc agccatggcc
     7321 accagccctt gcaggatgcg cagatcatca gcgcccagcg gctccgggcc gctgaactcg
     7381 atccgcttgc cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt gcgctcgccc
     7441 cgcttgaggg cacggaacag gccgggggcc agacagtgcg ccgggtcgtg ccggacgtgg
     7501 ctgaggctgt gcttgttctt aggcttcacc acggggcacc cccttgctct tgcgctgcct
     7561 ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc gatcagcgaa
     7621 cggtgcgcca tagttggcct tgctcacacc gaagcggacg aagaaccggc gctggtcgtc
     7681 gtccacaccc cattcctcgg cctcggcgct ggtcatgctc gacaggtagg actgccagcg
     7741 gatgttatcg accagtaccg agctgccccg gctggcctgc tgctggtcgc ctgcgcccat
     7801 catggccgcg cccttgctgg catggtgcag gaacacgata gagcacccgg tatcggcggc
     7861 gatggcctcc atgcgaccga tgacctgggc catggggccg ctggcgtttt cttcctcgat
     7921 gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg cgcgcttgag
     7981 gccgtcgaac cactccgggg ccatgatgtt gggcaggctg ccgatcagcg gctggatcag
     8041 caggccgtca gccacggctt gccgttcctc ggcgctgagg tgcgccccaa gggcgtgcag
     8101 gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg ggccggtggg
     8161 cagttcgccc acctccagca gatccggccc gcctgcaatc tgtgcggcca gttgcagggc
     8221 cagcatggat ttaccggcac caccgggcga caccagcgcc ccgaccgtac cggccaccat
     8281 gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca gaatattgat
     8341 aggcttatgg gtagccattg attgcctcct ttgcaggcag ttggtggtta ggcgctggcg
     8401 gggtcactac ccccgccctg cgccgctctg agttcttcca ggcactcgcg cagcgcctcg
     8461 tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt catgcgctcg
     8521 gcatatcgcg cttggcgtac agcgtcaggg ctggccagca ggtcgccggt ctgcttgtcc
     8581 ttttggtctt tcatatcagt caccgagaaa cttgccgggg ccgaaaggct tgtcttcgcg
     8641 gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga ctgaaaagcg
     8701 gccagcctcg gccttgtttg acgtataacc aaagccaccg ggcaaccaat agcccttgtc
     8761 acttttgatc aggtagaccg accctgaagc gcttttttcg tattccataa aacccccttc
     8821 tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa cgcaagcact acatgctgaa
     8881 atctggcccg cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg ccagctcggc
     8941 ccgcgcaagc tggacgctgg gcagacccat gaccttgctg acggtgcgct cgatgtaatc
     9001 cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg gcctcggcca tggccttgcc
     9061 gatttcctcg gcactgcggc cccggctggc cagcttctgc gcggcgataa agtcgcactt
     9121 gctgaggtca tcaccgaagc gcttgaccag cccggccatc tcgctgcggt actcgtccag
     9181 cgccgtgcgc cggtggcggc taagctgccg ctcgggcagt tcgaggctgg ccagcctgcg
     9241 ggccttctcc tgctgccgct gggcctgctc gatctgctgg ccagcctgct gcaccagcgc
     9301 cgggccagcg gtggcggtct tgcccttgga ttcacgcagc agcacccacg gctgataacc
     9361 ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc catagtggcg
     9421 gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg caatctgccc
     9481 ccgaagttca ccgcctgcgg cgtcggccac cttgacccat gcctgatagt tcttcgggct
     9541 ggtttccact accagggcag gctcccggcc ctcggctttc atgtcatcca ggtcaaactc
     9601 gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc tgatatacac
     9661 gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca cttcggcggc
     9721 tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga tatcgaagcg
     9781 ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt cgttcttcat
     9841 cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt caggtcgagc
     9901 aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag catcacggtt
     9961 agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc ggcgctgctc
    10021 acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc ccctgtctgg
    10081 cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt ctggctcatg
    10141 acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc gatctgctcc
    10201 gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat ggtctattgc
    10261 ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt tcagggccac
    10321 gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg gccccaggtg
    10381 aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa tgcgggcgtc
    10441 gtggccagcc cgctctaatg cccggttggc atggtcggcc catgcctcgc gggtctgctc
    10501 aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct ccggggtctt
    10561 gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat tgatccgctc
    10621 ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg ccagcgtata
    10681 cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg ccttctgctg
    10741 gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc gcccattggc
    10801 gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga actccggcat
    10861 gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc cttcgcgctg
    10921 gatgtagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg ttttcgccgt
    10981 aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc catgca
//
