LOCUS       CP082690               11509 bp    DNA     linear   BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Bredeney strain CVM
            N18S0406 plasmid pN18S0406-4.
ACCESSION   CP082690
VERSION     CP082690.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN11967990
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Bredeney
  ORGANISM  Salmonella enterica subsp. enterica serovar Bredeney
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11509)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2019) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 11509)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 313x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 14:41:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,842
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,604
            CDSs (with protein)               :: 4,604
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 45 of 118
            Pseudo Genes (incomplete)         :: 77 of 118
            Pseudo Genes (internal stop)      :: 19 of 118
            Pseudo Genes (multiple problems)  :: 21 of 118
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11509
                     /organism="Salmonella enterica subsp. enterica serovar
                     Bredeney"
                     /mol_type="genomic DNA"
                     /strain="CVM N18S0406"
                     /serovar="Bredeney"
                     /isolation_source="ground turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:134047"
                     /plasmid="pN18S0406-4"
                     /geo_loc_name="USA:MD"
                     /collection_date="2018"
                     /collected_by="FDA"
     gene            complement(<1..182)
                     /locus_tag="FH339_002270"
     CDS             complement(<1..182)
                     /locus_tag="FH339_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="UAH60889.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYA"
                     /name="QacE family quaternary ammonium compound efflux SMR
                     transporter"
     gene            complement(348..563)
                     /locus_tag="FH339_002275"
     CDS             complement(348..563)
                     /locus_tag="FH339_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAH60890.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(567..1052)
                     /locus_tag="FH339_002280"
     CDS             complement(567..1052)
                     /locus_tag="FH339_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAH60891.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKAVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(1027..1791)
                     /locus_tag="FH339_002285"
                     /pseudo=""
     CDS             complement(1027..1791)
                     /locus_tag="FH339_002285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1730..2053)
                     /locus_tag="FH339_002290"
     CDS             complement(1730..2053)
                     /locus_tag="FH339_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAH60900.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2692..2862
                     /locus_tag="FH339_002295"
     CDS             2692..2862
                     /locus_tag="FH339_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UAH60892.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     gene            complement(3071..3403)
                     /locus_tag="FH339_002300"
     CDS             complement(3071..3403)
                     /locus_tag="FH339_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="UAH60893.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(3571..3786)
                     /locus_tag="FH339_002305"
     CDS             complement(3571..3786)
                     /locus_tag="FH339_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAH60894.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3790..4275)
                     /locus_tag="FH339_002310"
     CDS             complement(3790..4275)
                     /locus_tag="FH339_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAH60895.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKAVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(4250..5014)
                     /locus_tag="FH339_002315"
                     /pseudo=""
     CDS             complement(4250..5014)
                     /locus_tag="FH339_002315"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4953..5276)
                     /locus_tag="FH339_002320"
     CDS             complement(4953..5276)
                     /locus_tag="FH339_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAH60901.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(6523..7002)
                     /locus_tag="FH339_002325"
     CDS             complement(6523..7002)
                     /locus_tag="FH339_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH60896.1"
                     /translation="MNLLLPRDIVEAVLNDKKTKNARVAKCDGSEFFLELPSMNADFPA
                     GKIILKLGDSGFYNKRTKSLEGAYGLRHIWDKHRVEIGATSAEDIVIFLESILLAGAEV
                     LIDPKKGQNKAIVVESGTGMMILELKKPNGEDPYYSIITAYDRKSHPGTKLHTLI"
                     /name="hypothetical protein"
     gene            7478..8029
                     /locus_tag="FH339_002330"
     CDS             7478..8029
                     /locus_tag="FH339_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAH60897.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(8228..8443)
                     /locus_tag="FH339_002335"
     CDS             complement(8228..8443)
                     /locus_tag="FH339_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UAH60898.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8447..8932)
                     /locus_tag="FH339_002340"
     CDS             complement(8447..8932)
                     /locus_tag="FH339_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UAH60899.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSAAMSQRTKGMLRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(8907..9671)
                     /locus_tag="FH339_002345"
                     /pseudo=""
     CDS             complement(8907..9671)
                     /locus_tag="FH339_002345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9610..9933)
                     /locus_tag="FH339_002350"
     CDS             complement(9610..9933)
                     /locus_tag="FH339_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UAH60902.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(3072..3403)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(2150..2341)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(5378..5564)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.263"
                     /coverage="89.0"
                     /evalue="2.7100000000000003e-47"
     Typing          complement(10035..10221)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.263"
                     /coverage="89.0"
                     /evalue="2.7100000000000003e-47"
     Typing          2321..2804
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          5544..6023
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.938"
                     /coverage="93.0"
                     /evalue="3.51e-174"
     Typing          10201..10680
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.733"
                     /coverage="93.0"
                     /evalue="1.6299999999999998e-172"
     Typing          complement(251..1740)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3474..4963)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.383"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8125..9620)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.583"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtaagcaa taccggtagg tatattttcc aatgtttggg aaagcagata aaaggcagcg
       61 caataactta ttgcagttcc tacagacggc cacaggcgtg taaatccttc tgaatatttc
      121 ataaaggtgg tgcctatgac ttcagccaca atagcccctc ccaggaaaat atatgtattc
      181 ataaaaatac ctcatgttga aaatttaaat atctttacat aaaactaatt aatagaaccc
      241 ggcttatcat agcgaaggac catgatgctg ccactgcgat cgttccagct ccagcgcttt
      301 ctggtgctgc accgcccgct cctgctgccg gtgagtttct atcgcgttca gcctttcgac
      361 aaccggcgca gctgctcgct caaactctgc acctgcctgc tcaagaccgt gacgcgctcg
      421 ttcagcgccg cgttctcccg ttgcataaga ccagacatcg tccgccattc cgcgaaggcg
      481 ttctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa tgcgctcagc
      541 aactgttttt ccagctccgt catgtgctat ttccccgcca gtatcatcca gctcgagtcc
      601 tctccgaact gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg ctcgccgcag
      661 gtcgagagct gtacgccgct gttcctctct gacagcatgg cctgcgtgct cttctgctcc
      721 cggatggtcg tatagttctc gagtatctgc tgcccctgcc accacagaat gcccgcgctc
      781 gacgctatca gcagcgcgga gaccagaacg atggtcagcc acgtctggct gaccatgcgc
      841 agcatccctt tcgtgcgctg gctcatggct gaggacagct tccggtcgtg gtcgaggatg
      901 gcggcgctga ttctcttctc gctttcgctc agttccgctc tgacagcctt ctcgtgctcg
      961 ctgaatgcgg ctttcagcat ctcgccggta gtctgctgct gcgctttcga tttctgctct
     1021 aagtccttcg ccagcgttaa aagactgttc atagatggct cccttcagtc ggatgtttcg
     1081 ccccccgtcc gggtcggcaa tgctgatgct gctttttgtg gtgcgcacca cctcaaaacc
     1141 tgcgctttcc agcgcctcag tgacgtcctg acgcgttttc agctccccgg atgaggcaag
     1201 ggcaagcagg ccgctcgtaa tcgcctgggc ggcttcctgc ttcgcttccg gcagcgcgga
     1261 aggcgagacc agcacccgcc ggttctccgg cgcgttcggg tcatgcagcc ccagcctgcc
     1321 gttcactatg gtctgccagg catcgatgcg cggacggtcg gcgcggtcgt aatacggctg
     1381 tagccgcttg ccggtcagaa gctccgtgtt cgggatcagg aagttcagct ccagccgccc
     1441 cttgtcccgg tgctcaaccc acagcacgct gtactggtct ttatcgagtc cgggcatcag
     1501 aacccgctcg aaactcgcca tcagcttttc gcgctggccg ggcggtaaat cctgttcggc
     1561 aaaagacaga acgccggaag tgtacttttt ggcgtagggc gaggcgtcga taagctcccg
     1621 gacttcatcc ggcttcccct gcagaacgct ggcgccgtcg cgctggcgat ctttacccag
     1681 cagataatcg accggcccgc cgccgccgcc gcgcccccga ggatgaaact taacgatcat
     1741 catcagcacc cttttccagt acggcatgcc gcaaccgctc gagtccggca tcgatggcca
     1801 tcagcgcggc gacaatctgc acgcggtcgt gtccgcttcc accaccggcg ttaacctggc
     1861 gggcaatctg gttaaggttg ttccccatgc cggcaagctg acgaagcagc gccggcgaga
     1921 tcgacggaag tttgccggcg cgtgcgggct tctcgtccag gcacgtctgc cgcatccagg
     1981 ccgcgagttg cttaccctcg cagcgctcca gcagacgccg gtgctcgtcc tcagtcaccc
     2041 acatggtgag cattttgctg cgtttgtctg ccaatccggc ctcctgataa cgccgggacg
     2101 gacgggaaat cttttcccgg tcggcacggt gttggacaag ccgcaggcgc ggcagtggtt
     2161 ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg
     2221 gctaaacatg ttatgacgga gtggcaattt atgaaagtgc ttcatgtgac aggggaaaaa
     2281 tgcggctgcc gggcgtaagt gcaaaatgca ggtggtgatg aaatgacgcc tcctcgctca
     2341 ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt taccagctca cagattacgg
     2401 gataagcaaa tgccgctcga agcaggaaag gccaagaaaa ggatgcggag ttgccgtttt
     2461 tccataggct ccgcccccct gacgagtatc acgaaaatga cgctcaaatc agcggtggcg
     2521 aaacccgatg cggcttaaag ataccaggcg tttccccctg gtggctccct cgtgctcccc
     2581 cgcattcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt
     2641 ccacgcctga aactcggttc cgggtaggca gttcgctcca ggatggactg tatgcacgaa
     2701 ccccccgttc agtccgacta ccacgcccgt tccggtaact atcaacttga gtccaacccg
     2761 gaaagacacg acaaatcgcc agtggcggta gccattggta actgggttca cggagcagag
     2821 attgtagaga atgcctacag agttcttgaa gtgaaagcct gagtgcggct acactgaaag
     2881 gacagtttag gtatctgcgc tccgtgaaag ccagttacca tcggaacaaa gagttggtag
     2941 ctctgaattc cggcaaacga accaccgttg gtagcggtgg tttttttgtt tacagggaaa
     3001 aagattacgc gcagaaagaa aggatctcaa ggaaatcctt ttgctttggc caatatgctt
     3061 cggtaaaaag ttaatgcgca gaagattttg aaaaaatatt aataaccaaa actccaacgc
     3121 aaataaggaa cattcctgca attgcgggcc aatcaagttt ttgaccgcta accaaccaac
     3181 cagctaggct tattagtaca attcccgcac cagaccaaat tgcgtaagca ataccggtag
     3241 gtatattttc caatgtttgg gaaagcagat aaaaggcagc gcaataactt attgcagttc
     3301 ctacagacgg ccacaggcgt gtaaatcctt ctgaatattt cataaaggtg gtgcctatga
     3361 cttcagccac aatagcccct cccaggaaaa tatatgtatt cataaaaata cctcatgttg
     3421 aaaaatttaa atatctttac ataacaacta attaatagaa cccggcttat catagcgaag
     3481 gaccatgatg ctgccactgc gatcgttcca gctccagcgc tttctggtgc tgcaccgccc
     3541 gctcctgctg ccggtgagtt tctatcgcgt tcagcctttc gacaaccggc gcagctgctc
     3601 gctcaaactc tgcacctgcc tgctcaagac cgtgacgcgc tcgttcagcg ccgcgttctc
     3661 ccgttgaata agaccagaca tcgtccgcca ttccgcgaag gcgttctccc actcgtccag
     3721 ccttttcgag tagtcctgct gtagctgctc taatgcgctc agcaactgtt tttccagctc
     3781 cgtcatgtgc tatttccccg ccagtatcat ccagctcgag tcctctccga actgtcccgc
     3841 ttccgggttc accctcacgc agcggcgtct ctgctcgccg caggtcgaga gctgtacgcc
     3901 gctgttcctc tctgacagca tggcctgcgt gctcttctgc tcccggatgg tcgtatagtt
     3961 ctcgagtatc tgctgcccct gccaccacag aatgcccgcg ctcgacgcta tcagcagcgc
     4021 ggagaccaga acgatggtca gccacgtctg gctgaccatg cgcagcatcc ctttcgtgcg
     4081 ctggctcatg gctgaggaca gcttccggtc gtggtcgagg atggcggcgc tgattctctt
     4141 ctcgctttcg ctcagttccg ctctgacagc cttctcgtgc tcgctgaatg cggctttcag
     4201 catctcgccg gtagtctgct gctgcgcttt cgatttctgc tctaagtcct tcgccagcgt
     4261 taaaagactg ttcatagatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg
     4321 caatgctgat gctgcttttt gtggtgcgca ccacctcaaa acctgcgctt tccagcgcct
     4381 cagtgacgtc ctgacgcgtt ttcagctccc cggatgaggc aagggcaagc aggccgctcg
     4441 taatcgcctg ggcggcttcc tgcttcgctt ccggcagcgc ggaaggcgag accagcaccc
     4501 gccggttctc cggcgcgttc gggtcatgca gccccagcct gccgttcacg atggtctgcc
     4561 aggcatcaat gcgcggacgg tcggcgcggt cgtaatacgg ctggagacgc ttgccggtca
     4621 gcagctccgt gttcgggatc aggaagttca gctccagccg ccccttatcc tggtgctcaa
     4681 cccacagcac gctgtactgg tctttatcga gtccgggcat cagaacccgt tcaaagctcg
     4741 ccatcagctt ttcgcgctgc ccgggcggta aatccttttc ggcaaaggac aagacgccgg
     4801 atgtgtactt ttttgcgtag ggcgaggcgt cgataagctc cctgacttct tccggcttcc
     4861 cctgcagaac ggtggcgccg tcgcgctgtc ggtctttccc cagcagataa tccaccggac
     4921 cggcgccacc gccgcgccct cgcgggtgaa acttaacgat catcgtctgc acccttttcc
     4981 agcacggcat gccgcagccg ctcgagtccg gcatctatgg ccatcagcgc ggcgacgacc
     5041 tgcacgcggt cgtgcccgct gccaccaccg gcgttaacct gccgggcaat ctggttgagg
     5101 ttgttcccca tgccggcaag ctgacgaagc agcgccggcg agagcgaggg aagcttgccg
     5161 gcgcgggcgg gtttctcgtc caggcacgtc tggcgcatcc aggctgcaag ctgtttaccg
     5221 tcgcagcgct ccagcagacg gcggtgctca tcttcggtca cccacatcgt gagcattttg
     5281 ctgcgtttgt ctgccaatcc ggcctcctga taacgccgag acgggcggga aatcttttcc
     5341 cggtcggcgc ggtgttggac aagccgcagg cgcgtcagtg gttttagcgg gtttcggggc
     5401 gcagccctga accagtcacg tagcgctagc ggagtgtata ctggcttagt atggtaaatc
     5461 gaagtggaaa ttcagggaag tgcttcatgt ggcaggtaaa aaattcagct gtggggcgta
     5521 agtgcgaaat gctggagacg atgaaatgac gcctcctcgc tcactcggtc gctacgctcc
     5581 tgccgtgaga ctgcggcggg cgttacctgc tcaagaatga cgagataaat agcgctcaaa
     5641 tcaagaagga aggggaaaag gatccggcgt tgccgttttt ccataggctc cgcccccctg
     5701 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     5761 gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt
     5821 ttaccggtgt cattccgctg ttatggccgc gtttatctca ttccacgcct gacactcagt
     5881 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     5941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cgaaaagaca cgacttaacg
     6001 ccactggcag cggccactgg taagcaggat gtgcgagaga tgtatcgaga gttcttgaag
     6061 tggaggcctt agtgaggcta cactggaagg acagtatttg gtgacatgtc tcgcacaaga
     6121 ctgttaccac ggttcagaaa cttgccggtt tactgaacct tcgaaaaacc accttgccgg
     6181 gtggtttttt cgtttacagg gagagagatt acgacgagag taaaacgatc acaaggagat
     6241 ctgaccgttt accagtgatt tggcttcgtt ttggtttcca ctgaacacca aaatagcact
     6301 ttttgcaaaa atatgagcag tgatagagtt gtattctgta tgtgtagaag ggcttggtat
     6361 gtaacctgaa ctctggattg gttacatatt tgtaggctac ttgtcggaaa ccagatttcg
     6421 acaatgcgtt gttaaggtca aagacctgtc ccgaaccgaa atgctaaggg aatgccacga
     6481 acaacactat tacgaagaag tcggctattt agccggcttt tttcatatta gggtatgaag
     6541 ttttgtacct ggatggctct tcctatcata agcagtaatg atgctgtagt acgggtcttc
     6601 gccgtttggt tttttcaatt ctagtatcat catccccgtt cctgactcta caacaatcgc
     6661 tttgttttgg ccttttttag gatctatgag cacttctgcc ccagctaata atatgctctc
     6721 caaaaagatg acaatatcct cagcggaagt agctcctatc tcgacacgat gcttgtccca
     6781 tatatgccta aggccatagg ctccctcgag actttttgtc cttttgttgt aaaaaccgga
     6841 atctcctagc ttgaggatga ttttccctgc gggaaagtct gcattcatgc ttggcagttc
     6901 tagaaaaaat tcacttccat cacacttagc cactcgtgca ttttttgttt tcttatcatt
     6961 aagaacagct tcaactatat cgcgtggaag taacaagttc acaaactaca cctctatagc
     7021 cttcactatt atgtttaggc gttaaatccc tttttgaagg gctgattcta acactgacgt
     7081 aatgagcaat ttccagtaaa tcttgataga aaaaaccagt tgtatttacc ataacattcc
     7141 ttgcccgcac cgctccggac gggttcagcg tgaaagcggc gttagcccgg taaaatcggc
     7201 agtaaactcc ccgctaaacc gcgcattctg cgcctgataa atttcacgtg gctgcatagc
     7261 catgcagcca cgtgaattgc tcatgctttc aatgtatatc ctcgtccaag cccctgtttt
     7321 aaccggttta tcaaaaaacc aaacagaaag ccttaccact aacaataaaa gaggtaggag
     7381 gactgcattt tatcgcataa aaaaacattt acaacacgat ttttatcgta tagattccat
     7441 aaaaaatgaa attcactcac ctattaaggg tttctatatg aaagatatga aagctgttgt
     7501 ggtttttacc gggaaggatt tgaacataat gagaactgag ggggggtcag gctactggca
     7561 tgccagaaca gacaggctca acgatgccga ttacctcata gcagtaagaa acagaaggga
     7621 aacgtgggca gtaaaagacc tggaacacgg cacagcattc ctgatcgcca aaattaccgg
     7681 gtgttttaaa agccctgatt acgacgacag gaacgtcata acctttgatg aatacgcaga
     7741 aattcacacg ccaaaagcat ggaaaatgct aaccgatggt cagcgctatc cggttgctta
     7801 cctgagcgca caggaagctt ttttacgtat tggggtaaca ccggaacaac tggaatggaa
     7861 gaaattccat ccttcatccc catcggttcc taatacagtg attcctggtc tggctgaaga
     7921 gaaaacagaa aaactctccc ttaacgaggc aattgaaaga gccaaaaagg atattagcaa
     7981 cgcaacaggg attgatagct cagccataac aataagcatc aagatctgat gaagcgtgaa
     8041 caacaagata gcagcctcat gttctagaac tgaacctgta cgccagaaac agcaagccca
     8101 tgcttttccg gcgcacagat tcaaagtgac ggaccgttgt acgttcgctg tcgctgagat
     8161 cgttccagct ccagcgcttt ctgatgctgc gccgcccgtt cctgctcacg atgtgcctct
     8221 atcacgttca gccttgtgac aaccggcgca gctgctcgct caaactctgc acctgcctgc
     8281 tcaagcccgt gacgcgctcg ctcagcgccg cgttctcccg ttgcataaga ccagacatcg
     8341 tccgccattc cgcgaaggcg ttctcccact cgtccagcct tttcgagtag tcctgctgta
     8401 gctgctctaa tgcgctcagc aactgttttt ccagctccgt catgtgctat ttccccgcca
     8461 gtatcatcca gctcgaatcc tctccgaacc gtcccgcttc cgggttcacc ctcacgcagc
     8521 ggcgtctctg ctctccgcag gtcgagagct gcacaccgct gttcctctcg gacagcatgg
     8581 cctgcgtgcg cttctgctcc cggatggtcg tgtaattgtc gagtatctgc tgcccctgcc
     8641 accacagaat acttgcgccc gatgctgtca gcagtgtaga caccaagacg atggtcagcc
     8701 acgtctggct gaccatccgc agcatacctt tcgtgcgctg gctcatggca gcggacagct
     8761 tcctgtcgtg gtcgaggatg gcggcgctga ttctcttctc gctttcgctc agttccgctc
     8821 tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct
     8881 gcgctttcga tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct
     8941 cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa tgctgatgct gctttttgtg
     9001 gtgcgtacca cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg gcgggttttc
     9061 agctccccgg acgaggctaa ggccagtaaa ccccgtgtaa ttgcctgagc ggcttcctgc
     9121 ttcgtttccg gcagcgcgga cggcgtgacc agcacccgcc ggttttcagg cgcgttcggg
     9181 tcatgcagcc ccagcctgcc gttcacgatg gtctgccagg catcaatgcg cggacggtcg
     9241 gcgcggtcgt aatacggctg gagacgcttg ccggtcagca gctccgtgtt cgggatcagg
     9301 aagttcagct ccagccgccc cttatcctgg tgctcaaccc acagcacgct gtactggtct
     9361 ttatcgagtc cgggcatcag aacccgttca aagctcgcca tcagcttttc gcgctgcccg
     9421 ggcggtaaat ccttttcggc aaaggacaag acgccggatg tgtacttttt tgcgtagggc
     9481 gaggcgtcga taagctccct gacttcttcc ggcttcccct gcagaacggt ggcgccgtcg
     9541 cgctgtcggt ctttccccag cagataatcc accggaccgg cgccaccgcc gcgccctcgc
     9601 gggtgaaact taacgatcat cgtctgcacc cttttccagc acggcatgcc gcagccgctc
     9661 gagtccggca tctatggcca tcagcgcggc gacgacctgc acgcggtcgt gcccgctgcc
     9721 accaccggcg ttaacctgcc gggcaatctg gttgaggttg ttccccatgc cggcaagctg
     9781 acgaagcagc gccggcgaga gcgagggaag cttgccggcg cgggcgggtt tctcgtccag
     9841 gcacgtctgg cgcatccagg ctgcaagctg tttaccgtcg cagcgctcca gcagacggcg
     9901 gtgctcatct tcggtcaccc acatcgtgag cattttgctg cgtttgtctg ccaatccggc
     9961 ctcctgataa cgccgagacg ggcgggaaat cttttcccgg tcggcgcggt gttggacaag
    10021 ccgcaggcgc gtcagtggtt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag
    10081 cgctagcgga gtgtatactg gcttagtatg gtaaatcgaa gtggaaattc agggaagtgc
    10141 ttcatgtggc aggtaaaaaa ttcagctgtg gggcgtaagt gcgaaatgct ggagacgatg
    10201 aaatgacgcc tcctcgctca ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt
    10261 tacctgctca agaatgacga gataaatagc gctcaaatca agaaggaagg ggaaaaggat
    10321 ccggcgttgc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
    10381 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggt
    10441 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
    10501 tggccgcgtt tatctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
    10561 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
    10621 tcgtcttgag tccaacccga aaagacacga cttaacgcca ctggcagcgg ccactgttaa
    10681 gcaggatgtg cgagagatgt atcgagagtt cttgaagtgg aggccttagt gaggctacac
    10741 tggaaggaca gtatttggtg acatgtctcg cacaacgttc agaaacttgc cggtttactg
    10801 aaccttcgaa aaaccacctt gccggtgtgg tttttcgttt acagggagag agattacgac
    10861 gagagtaaaa cgatcacaag gagatctgac gtttaccagt gatttggctt cgttttggtt
    10921 tccactgaac accaaaatag cactttttgc aaaaatatga gcagtgatag agttgtattc
    10981 tgtatgtgta gaagggcttg gtatgtaacc tgaactctgg attggttaca tatttgtagg
    11041 ctacttgtcg gaaaccagat ttcgacaatg cgttgttaag gtcaaagacc tgtcccgaac
    11101 cgaaatgcta agggaatgca cgaacaacac tattacgaag aagtcggcta tttagccggc
    11161 tttttcatat tagggtatga agttttgtac ctggaggctc ttcctatcat aagcagtaat
    11221 gatgctgtag tacgggtctt cgccgtttgg ttttttcaat tctagtatca tcatccccgt
    11281 tcctgactct acaacaatcg ctttgttttg gcctttttag gatctatggc acttctgccc
    11341 cagctaataa tatgctctcc aaaaagatga caatatcctc agcggaagta gctcctatct
    11401 cgacacgatg cttgtcccat aatgcctaag gccataggct ccctcgagac tttttgtcct
    11461 tttgttgtaa aaaccggaat ctcctagctt gaggatgatt ttccctgcg
//
