LOCUS       CP082661               11385 bp    DNA     linear   BCT 09-SEP-2021
DEFINITION  Salmonella enterica subsp. enterica serovar Heidelberg strain CVM
            N16S098 plasmid pN16S098-3.
ACCESSION   CP082661
VERSION     CP082661.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN08113977
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Heidelberg
  ORGANISM  Salmonella enterica subsp. enterica serovar Heidelberg
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Li,C., Tyson,G., Hsu,C.-H., Harrison,L., Strain,E., Tran,T.-T.,
            Tillman,G., Dessai,U., McDermott,P. and Zhao,S.
  TITLE     Long-read sequencing reveals evolution and acquisition of
            antimicrobial resistance and virulence genes in Salmonella enterica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11385)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 11385)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2020
            Assembly Method :: SMRTLink Microbial Assembly v. SMRTLink9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 816x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2021 17:26:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,839
            Genes (coding)                    :: 4,719
            CDSs (with protein)               :: 4,719
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 46 of 120
            Pseudo Genes (incomplete)         :: 77 of 120
            Pseudo Genes (internal stop)      :: 21 of 120
            Pseudo Genes (multiple problems)  :: 20 of 120
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Salmonella enterica subsp. enterica serovar
                     Heidelberg"
                     /mol_type="genomic DNA"
                     /strain="CVM N16S098"
                     /serovar="Heidelberg"
                     /isolation_source="Chicken legs"
                     /sub_species="enterica"
                     /db_xref="taxon:611"
                     /plasmid="pN16S098-3"
                     /geo_loc_name="USA:PA"
                     /collection_date="2016"
                     /collected_by="FDA"
     gene            1..289
                     /locus_tag="CWX60_024735"
                     /pseudo=""
     CDS             1..289
                     /locus_tag="CWX60_024735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlB family
                     antitoxin"
                     /name="type II toxin-antitoxin system RnlB family
                     antitoxin"
     gene            complement(526..849)
                     /locus_tag="CWX60_024740"
     CDS             complement(526..849)
                     /locus_tag="CWX60_024740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="UAG24773.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            1134..1328
                     /locus_tag="CWX60_024745"
     CDS             1134..1328
                     /locus_tag="CWX60_024745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UAG24774.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2193..3233
                     /gene="lsoA"
                     /locus_tag="CWX60_024750"
     CDS             2193..3233
                     /gene="lsoA"
                     /locus_tag="CWX60_024750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="UAG24775.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            3230..3610
                     /gene="lsoB"
                     /locus_tag="CWX60_024755"
     CDS             3230..3610
                     /gene="lsoB"
                     /locus_tag="CWX60_024755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="UAG24776.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(3845..4168)
                     /locus_tag="CWX60_024760"
     CDS             complement(3845..4168)
                     /locus_tag="CWX60_024760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="UAG24777.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            4453..4647
                     /locus_tag="CWX60_024765"
     CDS             4453..4647
                     /locus_tag="CWX60_024765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UAG24778.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5512..6552
                     /gene="lsoA"
                     /locus_tag="CWX60_024770"
     CDS             5512..6552
                     /gene="lsoA"
                     /locus_tag="CWX60_024770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="UAG24779.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            6549..6929
                     /gene="lsoB"
                     /locus_tag="CWX60_024775"
     CDS             6549..6929
                     /gene="lsoB"
                     /locus_tag="CWX60_024775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="UAG24780.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(7164..7487)
                     /locus_tag="CWX60_024780"
     CDS             complement(7164..7487)
                     /locus_tag="CWX60_024780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="UAG24781.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            7772..7966
                     /locus_tag="CWX60_024785"
     CDS             7772..7966
                     /locus_tag="CWX60_024785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UAG24782.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8831..9871
                     /gene="lsoA"
                     /locus_tag="CWX60_024790"
     CDS             8831..9871
                     /gene="lsoA"
                     /locus_tag="CWX60_024790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="UAG24783.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            9868..10248
                     /gene="lsoB"
                     /locus_tag="CWX60_024795"
     CDS             9868..10248
                     /gene="lsoB"
                     /locus_tag="CWX60_024795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="UAG24784.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(10483..10806)
                     /locus_tag="CWX60_024800"
     CDS             complement(10483..10806)
                     /locus_tag="CWX60_024800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="UAG24785.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            11092..11262
                     /locus_tag="CWX60_024805"
                     /pseudo=""
     CDS             11092..11262
                     /locus_tag="CWX60_024805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(859..1099)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          complement(4178..4418)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          complement(7497..7737)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          complement(10816..11056)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          1358..1887
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          4677..5206
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          7996..8525
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(530..849)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.2e-62"
     Typing          complement(3849..4168)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.2e-62"
     Typing          complement(7168..7487)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.2e-62"
     Typing          complement(10487..10806)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.2e-62"
ORIGIN
        1 gcccgagcga atttttctca aaatcgaatc cgacttaaag aaaaagaaaa gcaagggtga
       61 tgttttttga tttaataatt cctaacggtg gaaaaaagat cgttacgtct atacgtcatt
      121 taatggcgag aagttttcaa gttacacatt aaacaaagtt acgaaaactg atgaatataa
      181 tgatttatct gagctctcgg cttcgttctt taaaaaaaac tttgataaga tcaacgtaaa
      241 ccttctatcc aaagccacat catttgcttt gaaaaaaggc attccaatat atatcaagcc
      301 agaagacatt taccataacg tctcattacg cgcaccgcca aaacgcgctg aacgcgattc
      361 tgacgcagac accgcaaaaa gagatgtttt ccccggggta aaccggaaaa atgcgtcagg
      421 atcgctttca gctcactgca tagctatgca tgaaagtgaa tggcgatcgg tttgggggct
      481 tacggcgttc ataccgtctg ttttcgacag tttctctccg ggaagctaat ctgccataag
      541 cctggataac agggcacggt gatactccgt aatagcgatc agcacctccg catactctct
      601 gtccccgaca cgcttgccgt cgataaagag ttttttctgc aacgccccca gtcgcataac
      661 ttctttcagc acccggtcat cagtcagtga gttaactttc ttaccgagag ctgccgcccg
      721 taaaaaacca gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc
      781 ttctgccgtc agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg
      841 ttttgtcata aaacatggct ccgtatctga catggggtgt cggggcgaag ccctgaccag
      901 ggtaattgta atagcgtgca tgtatgcgcg gtataacaat tgcacatcct gtcctgtcag
      961 caagctcgaa ttaccgacaa aggcacggca gttttttggc agtgacagta ttgtcaggca
     1021 gaaatacccg tcaaagcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt
     1081 acgcgatata atcacacaac gcagatgaca gacagaacgg agaaaacgaa aagatgaaca
     1141 agcagcagca agccgtactc aacatggcag gattcataaa aagccagagc ctgacgctgc
     1201 tcgaaaagct ggacgcactc gatgctgacg aacaggctgc catgtgtgag aagctgcacg
     1261 aactcgcaga agaactccag aacagcatcc agacacgctt tgaagtagaa aatagcacag
     1321 agagataact ctcaggacag gatgccgaac ggcacaacag agcgtaagga gaaaatgtca
     1381 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1441 gcgagcggtg tcagctcact caaaagcggt aatacggtta tccacagaat caggggataa
     1501 agccggaaag aacatgtgag caaaaagcaa agcaccggaa gaagccaacg ccgcaggcgt
     1561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agccagaggt
     1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1741 gcgtggcgct ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct
     1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccattg
     1921 gtaactgatt tagaggagtt tgtcttgaag ttatgcacct gttaaggcta aactgaaaga
     1981 acagattttg gtgactgcgc tcctccaagc cagttacctt ggttcaaaga gttggtagct
     2041 cagcgaacct tgagaaaacc accgttggta gcggtggttt ttctttattt atgagatgat
     2101 gaatcaatcg gtctatcaag tcaacgaaca gctattccgt tgacaaaaaa acaagatctg
     2161 tacatattca aaaaaaacaa cggattgacc caatggcaca gaacccgttt aaagcactaa
     2221 atatcaatat tgacaagatt gagtctgctc tgacgcagaa tggcgtcaca aactattcct
     2281 ctaatgtaaa aaacgaaaga gaaactcaca tatctggcac atataaagga atagacttct
     2341 taataaaact aatgccatca ggcggaaata ccactatcgg aagagcgtct gggcaaaata
     2401 acacttactt tgatgaaatc gccttgatta taaaagaaaa ctgtttgtat tcagacacaa
     2461 agaactttga atacaccatt ccaaaattca gtgatgatga cagggcaaat ctatttgaat
     2521 ttctttctga agaggggata acaataacag aagataataa taacgatcct aattgtaaac
     2581 accaatatat tatgaccacc agcaatggtg acagggtcag ggcaaaaatt tacaagcgcg
     2641 gctctattca attccaagga aaataccttc aaatcgcgag tttgattaac gatttcatgt
     2701 gctcaatact aaacatgaaa gagattgtcg aacaaaaaaa taaagaattt aatgttgaca
     2761 taaaaaaaga aactattgag tccgagttgc atagcaaact accaaaaagc atcgataaaa
     2821 tccatgaaga tatcaaaaaa cagctatcat gctcgctaat aatgaaaaaa atagatgtcg
     2881 aaatggaaga ttactcaaca tactgcttct ctgcattaag agccatagaa ggctttatat
     2941 atcaaatact taatgatgtt tgcaatccat catcatcaaa gaaccttggc gaatacttca
     3001 ctgaaaacaa acccaaatat ataatcagag aaatacacca agaaactata aatggtgaaa
     3061 tagcggaagt tttgtgtgaa tgctacactt actggcatga gaacaggcat ggtttgtttc
     3121 atatgaaacc aggaatagct gacacgaaga caattaacaa attagaatca atcgcaatca
     3181 tcgataccgt ttgccaatta atagatggtg gcgtagctag gttgaaatta tgaaaaaaga
     3241 taaaaaatat caaatagaag caataaaaaa taaagataaa actttattta ttgtctatgc
     3301 tactgatatt tatagcccga gcgaattttt ctcaaaaatc gaatccgact taaagaaaaa
     3361 gaaaagcaag ggtgatgttt tttttgattt aataattcct aacggtggaa aaaaagatcg
     3421 ttacgtctat acgtcattta atggcgagaa gttttcaagt tacacattaa acaaagttac
     3481 gaaaactgat gaatataatg atttatctga gctctcggct tcgttcttta aaaaaaactt
     3541 tgataagatc aacgtaaacc ttctatccaa agccacatca tttgctttga aaaaaggcat
     3601 tccaatataa tatcaagcca gaagacattt accataacgt ctcattacgc gcaccgccaa
     3661 aacgcgctga acgcgattct gacgcagaca ccgcaaaaag agatgttttc cccggggtaa
     3721 accggaaaaa tgcgtcagga tcgctttcag ctcactgcat agctatgcat gaaagtgaat
     3781 ggcgatcggt ttgggggctt acggcgttca taccgtctgt tttcgacagt ttctctccgg
     3841 gaagctaatc tgccataagc ctggataaca gggcacggtg atactccgta atagcgatca
     3901 gcacctccgc atactctctg tccccgacac gcttgccgtc gataaagagt tttttctgca
     3961 acgcccccag tcgcataact tctttcagca cccggtcatc agtcagtgag ttaactttct
     4021 taccgagagc tgccgcccgt aaaaaaccag aaacggtctt gccgcattca gcagcccttt
     4081 ttctgatttc ctggtcttct tctgccgtca gacgaacagg gcgactgata gcccgcctgc
     4141 gcgtattact tccacttcgt tttgtcataa aacatggctc cgtatctgac atggggtgtc
     4201 ggggcgaagc cctgaccagg gtaattgtaa tagcgtgcat gtatgcgcgg tataacaatt
     4261 gcacatcctg tcctgtcagc aagctcgaat taccgacaaa ggcacggcag ttttttggca
     4321 gtgacagtat tgtcaggcag aaatacccgt caaagcgtca gacggcagaa taccggcggt
     4381 aaaacagtcg aaaagtggta cgcgatataa tcacacaacg cagatgacag acagaacgga
     4441 gaaaacgaaa agatgaacaa gcagcagcaa gccgtactca acatggcagg attcataaaa
     4501 agccagagcc tgacgctgct cgaaaagctg gacgcactcg atgctgacga acaggctgcc
     4561 atgtgtgaga agctgcacga actcgcagaa gaactccaga acagcatcca gacacgcttt
     4621 gaagtagaaa atagcacaga gagataactc tcaggacagg atgccgaacg gcacaacaga
     4681 gcgtaaggag aaaatgtcac ataaggcgct cttccgcttc ctcgctcact gactcgctac
     4741 gctcggtcgt tcgactgcgg cgagcggtgt cagctcactc aaaagcggta atacggttat
     4801 ccacagaatc aggggataaa gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag
     4861 aagccaacgc cgcaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4921 cgacgctcaa gccagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4981 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5041 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcagt
     5101 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5161 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5221 ccactggcag cagccattgg taactgattt agaggagttt gtcttgaagt tatgcacctg
     5281 ttaaggctaa actgaaagaa cagattttgg tgactgcgct cctccaagcc agttaccttg
     5341 gttcaaagag ttggtagctc agcgaacctt gagaaaacca ccgttggtag cggtggtttt
     5401 tctttattta tgagatgatg aatcaatcgg tctatcaagt caacgaacag ctattccgtt
     5461 gacaaaaaaa caagatctgt acatattcaa aaaaaacaac ggattgaccc aatggcacag
     5521 aacccgttta aagcactaaa tatcaatatt gacaagattg agtctgctct gacgcagaat
     5581 ggcgtcacaa actattcctc taatgtaaaa aacgaaagag aaactcacat atctggcaca
     5641 tataaaggaa tagacttctt aataaaacta atgccatcag gcggaaatac cactatcgga
     5701 agagcgtctg ggcaaaataa cacttacttt gatgaaatcg ccttgattat aaaagaaaac
     5761 tgtttgtatt cagacacaaa gaactttgaa tacaccattc caaaattcag tgatgatgac
     5821 agggcaaatc tatttgaatt tctttctgaa gaggggataa caataacaga agataataat
     5881 aacgatccta attgtaaaca ccaatatatt atgaccacca gcaatggtga cagggtcagg
     5941 gcaaaaattt acaagcgcgg ctctattcaa ttccaaggaa aataccttca aatcgcgagt
     6001 ttgattaacg atttcatgtg ctcaatacta aacatgaaag agattgtcga acaaaaaaat
     6061 aaagaattta atgttgacat aaaaaaagaa actattgagt ccgagttgca tagcaaacta
     6121 ccaaaaagca tcgataaaat ccatgaagat atcaaaaaac agctatcatg ctcgctaata
     6181 atgaaaaaaa tagatgtcga aatggaagat tactcaacat actgcttctc tgcattaaga
     6241 gccatagaag gctttatata tcaaatactt aatgatgttt gcaatccatc atcatcaaag
     6301 aaccttggcg aatacttcac tgaaaacaaa cccaaatata taatcagaga aatacaccaa
     6361 gaaactataa atggtgaaat agcggaagtt ttgtgtgaat gctacactta ctggcatgag
     6421 aacaggcatg gtttgtttca tatgaaacca ggaatagctg acacgaagac aattaacaaa
     6481 ttagaatcaa tcgcaatcat cgataccgtt tgccaattaa tagatggtgg cgtagctagg
     6541 ttgaaattat gaaaaaagat aaaaaatatc aaatagaagc aataaaaaat aaagataaaa
     6601 ctttatttat tgtctatgct actgatattt atagcccgag cgaatttttc tcaaaaatcg
     6661 aatccgactt aaagaaaaag aaaagcaagg gtgatgtttt ttttgattta ataattccta
     6721 acggtggaaa aaaagatcgt tacgtctata cgtcatttaa tggcgagaag ttttcaagtt
     6781 acacattaaa caaagttacg aaaactgatg aatataatga tttatctgag ctctcggctt
     6841 cgttctttaa aaaaaacttt gataagatca acgtaaacct tctatccaaa gccacatcat
     6901 ttgctttgaa aaaaggcatt ccaatataat atcaagccag aagacattta ccataacgtc
     6961 tcattacgcg caccgccaaa acgcgctgaa cgcgattctg acgcagacac cgcaaaaaga
     7021 gatgttttcc ccggggtaaa ccggaaaaat gcgtcaggat cgctttcagc tcactgcata
     7081 gctatgcatg aaagtgaatg gcgatcggtt tgggggctta cggcgttcat accgtctgtt
     7141 ttcgacagtt tctctccggg aagctaatct gccataagcc tggataacag ggcacggtga
     7201 tactccgtaa tagcgatcag cacctccgca tactctctgt ccccgacacg cttgccgtcg
     7261 ataaagagtt ttttctgcaa cgcccccagt cgcataactt ctttcagcac ccggtcatca
     7321 gtcagtgagt taactttctt accgagagct gccgcccgta aaaaaccaga aacggtcttg
     7381 ccgcattcag cagccctttt tctgatttcc tggtcttctt ctgccgtcag acgaacaggg
     7441 cgactgatag cccgcctgcg cgtattactt ccacttcgtt ttgtcataaa acatggctcc
     7501 gtatctgaca tggggtgtcg gggcgaagcc ctgaccaggg taattgtaat agcgtgcatg
     7561 tatgcgcggt ataacaattg cacatcctgt cctgtcagca agctcgaatt accgacaaag
     7621 gcacggcagt tttttggcag tgacagtatt gtcaggcaga aatacccgtc aaagcgtcag
     7681 acggcagaat accggcggta aaacagtcga aaagtggtac gcgatataat cacacaacgc
     7741 agatgacaga cagaacggag aaaacgaaaa gatgaacaag cagcagcaag ccgtactcaa
     7801 catggcagga ttcataaaaa gccagagcct gacgctgctc gaaaagctgg acgcactcga
     7861 tgctgacgaa caggctgcca tgtgtgagaa gctgcacgaa ctcgcagaag aactccagaa
     7921 cagcatccag acacgctttg aagtagaaaa tagcacagag agataactct caggacagga
     7981 tgccgaacgg cacaacagag cgtaaggaga aaatgtcaca taaggcgctc ttccgcttcc
     8041 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtgtc agctcactca
     8101 aaagcggtaa tacggttatc cacagaatca ggggataaag ccggaaagaa catgtgagca
     8161 aaaagcaaag caccggaaga agccaacgcc gcaggcgttt ttccataggc tccgcccccc
     8221 tgacgagcat cacaaaaatc gacgctcaag ccagaggtgg cgaaacccga caggactata
     8281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8401 acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8521 ggtaagacac gacttatcgc cactggcagc agccattggt aactgattta gaggagtttg
     8581 tcttgaagtt atgcacctgt taaggctaaa ctgaaagaac agattttggt gactgcgctc
     8641 ctccaagcca gttaccttgg ttcaaagagt tggtagctca gcgaaccttg agaaaaccac
     8701 cgttggtagc ggtggttttt ctttatttat gagatgatga atcaatcggt ctatcaagtc
     8761 aacgaacagc tattccgttg acaaaaaaac aagatctgta catattcaaa aaaaacaacg
     8821 gattgaccca atggcacaga acccgtttaa agcactaaat atcaatattg acaagattga
     8881 gtctgctctg acgcagaatg gcgtcacaaa ctattcctct aatgtaaaaa acgaaagaga
     8941 aactcacata tctggcacat ataaaggaat agacttctta ataaaactaa tgccatcagg
     9001 cggaaatacc actatcggaa gagcgtctgg gcaaaataac acttactttg atgaaatcgc
     9061 cttgattata aaagaaaact gtttgtattc agacacaaag aactttgaat acaccattcc
     9121 aaaattcagt gatgatgaca gggcaaatct atttgaattt ctttctgaag aggggataac
     9181 aataacagaa gataataata acgatcctaa ttgtaaacac caatatatta tgaccaccag
     9241 caatggtgac agggtcaggg caaaaattta caagcgcggc tctattcaat tccaaggaaa
     9301 ataccttcaa atcgcgagtt tgattaacga tttcatgtgc tcaatactaa acatgaaaga
     9361 gattgtcgaa caaaaaaata aagaatttaa tgttgacata aaaaaagaaa ctattgagtc
     9421 cgagttgcat agcaaactac caaaaagcat cgataaaatc catgaagata tcaaaaaaca
     9481 gctatcatgc tcgctaataa tgaaaaaaat agatgtcgaa atggaagatt actcaacata
     9541 ctgcttctct gcattaagag ccatagaagg ctttatatat caaatactta atgatgtttg
     9601 caatccatca tcatcaaaga accttggcga atacttcact gaaaacaaac ccaaatatat
     9661 aatcagagaa atacaccaag aaactataaa tggtgaaata gcggaagttt tgtgtgaatg
     9721 ctacacttac tggcatgaga acaggcatgg tttgtttcat atgaaaccag gaatagctga
     9781 cacgaagaca attaacaaat tagaatcaat cgcaatcatc gataccgttt gccaattaat
     9841 agatggtggc gtagctaggt tgaaattatg aaaaaagata aaaaatatca aatagaagca
     9901 ataaaaaata aagataaaac tttatttatt gtctatgcta ctgatattta tagcccgagc
     9961 gaatttttct caaaaatcga atccgactta aagaaaaaga aaagcaaggg tgatgttttt
    10021 tttgatttaa taattcctaa cggtggaaaa aaagatcgtt acgtctatac gtcatttaat
    10081 ggcgagaagt tttcaagtta cacattaaac aaagttacga aaactgatga atataatgat
    10141 ttatctgagc tctcggcttc gttctttaaa aaaaactttg ataagatcaa cgtaaacctt
    10201 ctatccaaag ccacatcatt tgctttgaaa aaaggcattc caatataata tcaagccaga
    10261 agacatttac cataacgtct cattacgcgc accgccaaaa cgcgctgaac gcgattctga
    10321 cgcagacacc gcaaaaagag atgttttccc cggggtaaac cggaaaaatg cgtcaggatc
    10381 gctttcagct cactgcatag ctatgcatga aagtgaatgg cgatcggttt gggggcttac
    10441 ggcgttcata ccgtctgttt tcgacagttt ctctccggga agctaatctg ccataagcct
    10501 ggataacagg gcacggtgat actccgtaat agcgatcagc acctccgcat actctctgtc
    10561 cccgacacgc ttgccgtcga taaagagttt tttctgcaac gcccccagtc gcataacttc
    10621 tttcagcacc cggtcatcag tcagtgagtt aactttctta ccgagagctg ccgcccgtaa
    10681 aaaaccagaa acggtcttgc cgcattcagc agcccttttt ctgatttcct ggtcttcttc
    10741 tgccgtcaga cgaacagggc gactgatagc ccgcctgcgc gtattacttc cacttcgttt
    10801 tgtcataaaa catggctccg tatctgacat ggggtgtcgg ggcgaagccc tgaccagggt
    10861 aattgtaata gcgtgcatgt atgcgcggta taacaattgc acatcctgtc ctgtcagcaa
    10921 gctcgaatta ccgacaaagg cacggcagtt ttttggcagt gacagtattg tcaggcagaa
    10981 atacccgtca aagcgtcaga cggcagaata ccggcggtaa aacagtcgaa aagtggtacg
    11041 cgatataatc acacaacgca gatgacagac agaacgagag aaaacgaaaa gatgaacaag
    11101 cagcagcaag ccgtactcaa catggcagga ttcataaaaa gccagagcct gacgctgctc
    11161 gaaaagctgg acgcactcga tgctgaaaca ggctgccatg tgtgagaagc tgcacgaact
    11221 cgcaagaaac tccagaacag catccagaca cgcttgaagt agaaaatagc acagagagat
    11281 aactctcagg acaggatgcc gaacggcaca acagagcgta aggagaaaat gtcactaagg
    11341 cgctcttccg cttcctcgct catgactcgc tacgctcggt cgttc
//
